Intshayelelo Kwi-IL yomntu - Iikhilomitha ezi-2
● Inkcazo kunye nokubaluleka
I-Enterleukin - 2 (IL - 2) yi-cytofini ye-pivotali emkhosini yamandla, ngokuyintloko ibandakanyeke kumgaqo weeseli ezimhlophe zegazi, ezibaluleke kakhulu kwimpendulo yomzimba omzimba. I-IL yomntu - Iikhithi ezi-2 zizixhobo ezibonakalayo zisetyenziswa kuzo zombini iindlela zophando kunye noseto lwekliniki ukulinganisa uxinzelelo lwe-IL - 2 kwiisampulu ezahlukeneyo zebhayoloji. Ezi kokiat zibalulekile ekuqhubeni ukuqonda kwethu ukuqonda kwethu iimpendulo zokhuseleko lomzimba kunye nokuphuhliswa kweendlela ezintsha zonyango, ukuphucula ukusebenza kwezicelo zonyango.
Ukubaluleka kwe-IL - Ukufumanisa oku-2 kunyulwe kwiinkalo ezininzi, kubandakanywa amasimology kunye ne-oncology, apho kuqonda imo yokusebenza komzimba kunyango. Njengoko ishishini le-bietech livele, imfuno ye-Il ethembekileyo, ethembekileyo, nenezinto ezisebenzayo zeKits zeKitss, ukuqhuba ezintsha kubavelisi kunye nabathengisi kwihlabathi liphela.
● Iindidi zeesampulu zebhayoloji ezisetyenzisiweyo
Umntu ul - I-2 ikhithiS unokusetyenziswa kwiisampulu ezahlukeneyo zebhayoloji, kubandakanya i-serum, iplasma, inkcubeko yeselula, negazi liphela. Uhlobo ngalunye lwesampulu lubonelela ngolwazi olukhethekileyo kunye nemiceli mngeni, ukwenza ukubaluleka kokufumana i-kit pit ibaluleke kakhulu kwinto yayo. Umzekelo, ukuhlalutya inkcubeko yeselula kunokubonelela ngolwazi oluneenkcukacha kwimveliso ye-cytokine kwi-vitro, ebaluleke ngokukodwa kwizifundo ezikhoyo ze-imbumbululo.
Indlela ye-IL - Ukufunyanwa kwe-2
● Indima yee-antibodies kunye ne-enzymes
I-CORE ye-IL yomntu - Iikhithi ezi-2 zixhomekeke ekusebenziseni ii-antibodies kunye nee-enzymes zokufumanisa kwaye ziqwalasele il - iimolekyuli ezi-2. Ngokwesiqhelo, ezi kits zisebenzisa isandwich Elisa (i-enzyme - i-Intertorbent Assay. Kule ndlela, il - 2 Ukhona kwisampulu kufakwe phakathi kwee-antibodies ezimbini: i-antids anti youbamba i-antids kumgangatho oqinileyo kunye nokufunyanwa kwe-antidmedme. I-enzyme iqala ngokufakelwa kwendawo yokuvelisa utshintsho olunokubakho olunokuthi lulinganiswe i-spectramomestly.
Le ntlungu yendlela kunye nobungqina buxhomekeke kakhulu kumgangatho wee-antibodies ezisetyenzisiweyo, yiyo loo nto ukukhetha i-Il yomntu ofanelekileyo - I-2 Umngenisi we-TIT 2 Abavelisi abadumileyo baqinisekise ukuba i-antibodies ihlolwe ngokungqongqo kwaye iqinisekisiwe ukuba ibonelele ngeziphumo ezinokuthenjwa.
● Ubunzulu bemibala kunye ne-il - 2
Ilitye leliko lokulinganisa il - Amanqanaba ama-2 kwiisampuli ze-kits zokufumanisa iiKits eziboniswayo yi-enzyme - Uphendule kakhulu. Olu tshintsho lwemibalabala luhambelana ngokuthe ngqo kwi-Oction ye-IL - 2 Kwisampulu. Ngokulinganisa i-avelocty kwi-wavelength ekhethekileyo, abaphandi banokuchonga ngokuchanekileyo i-IL - 2 Ukugxininiswa kwe-curve esemgangathweni ekwindawo yokugxininiswa.
Izicelo ze-IL - 2 Ukufunyanwa kwe-2
● Ummiselo woPhuculo lweSakhiwo
Il - 2 inendima esisiseko kuphuhliso kunye nokwanda kweeseli ze-T, ezibaluleke kakhulu ekuzigonyazeni. I-IL yomntu - Iikhithi ezi-2 zibalulekile ekuphandeni iimpendulo zeselfowuni, zibonelela ngokuqonda kummiselo wendlela yomzimba. Oku kunefuthe kungekuphelanga kuphando olusisiseko kuphela kodwa kunye nokuphuhlisa unyango olujolise kumagorha akhuselweyo - Ukuphazamiseka okunxulumene noko.
● Unyango lwe-canotherapy kunye nempendulo yomzimba
Indima ye-IL - 2 Ukukhuthaza iimpendulo zokhuselo zidityaniswe kunyango lomhlaza, ngakumbi kunyango olujolise ekuphumeni kwamathumba. I-IL yomntu - Iikhithi ezi-2 zibalulekile kwezi seto, zivumela ukubekwa kweliso kwi-intection yezinto ezivunyiweyo kunye neempendulo zonyango, ngaloo ndlela ziququzelela iprogramu yonyango.
Amagqabantshintshi e-IL - 2 Iindidi zokufumana
● Iikhilomitha ze-Elpot: imvakalelo kunye nendlela yokusebenza
I-Elzpot (i-enzyme - I-Ismosospot) i-Ismosospt) i-Asdumes idume ngemvakalelo yabo, ekwazi ukufumana i-cytokines kwi-Sytokines engatshatanga - Inqanaba leseli. I-IL yomntu - Iikhithi ezimbini ezi-2 ziluncedo kakhulu ekuchongeni il - Iiseli ezi-2 ezifihlayo ngaphakathi kwabemi, zibonelela ngohlalutyo lwabantu, ukubonelela ngohlalutyo lobungakanani bempendulo yomzimba. Oku kuvakalelwa kukhenyuzo oluphezulu kubaluleke kakhulu kuvavanyo lwe-Inmological, ngakumbi xa kuchongwa iimpendulo zeCytoskine -
● I-EISAKIS: Iindlela ze-Assay Assay
I-ELISAIKIS ingabasebenzi bokuhlolwa kwe-cytokiine, ukuhambisa indlela eyomeleleyo kunye nethe ngqo yokuqinisekisa il - 2 kwiindidi ezininzi zesampulu. Abantu i-Il - 2 I-ELIIKS ithanda ukuthembeka kwabo kunye nokusetyenziswa kwayo, kokwenza ukuba kubekho isitayile kwiilabhoratri eziphandayo, ukusebenza kwesifo, kunye nesifo se-pathogenesis.
Iimpawu zeKhispot ye-Elpot kunye neziBonelelo
● I-munplate - I-Assay esekwe
Ifomathi ye-MicroPlate ye-Elrot Assays ivumela ukuphakama - Uhlalutyo lwegalelo, oluyingenelo kwizifundo ezikhulu. Umthamo wokuvavanya iisampulu ezininzi uphucula ukomelela kweenkcukacha-manani kwaye unciphisa umahluko, ukubonelela ngedatha ethembekileyo yokuthembeka kwiimeko ezininzi zokuvavanywa.
● Imvakalelo ephezulu yeempendulo ezisezantsi
Enye yeempawu zokuzimela kwe-ElIspot yeKits kukukwazi ukubona iimpendulo zeselfowuni ezinqabileyo, ezibaluleke kakhulu kuPumology apho iphantsi apho iphantsi - imicimbi ephindaphindiweyo inokuba nefuthe elibalulekileyo lebhayoloji. Olu luvo lwenza isixhobo esihle kakhulu kuphando lonyango kunye nokuphuhliswa kwemihlambi ye-novelopa.
Ulwazi lwe-Elsa Kit luchaziwe
● Indlela yokutsalwa kweSandwich Elisa
I-Sandwich Elisa yeyona ndlela iphambili yeyona ndlela isetyenzisiweyo kwi-IL yomntu - Iikhilomitha ezi-2 ngenxa yobunye kunye nemvakalelo. Obu buchule buquka ukubopha kwe-IL: 2 ukuya kwi-antidd thes, kulandele ukufumanisa nge-Enzyme yesibini - I-antid antids, i-theipiminal, i-2 yoxinzelelo.
● Ukulinganisa i-IL - 2 kwiPlasma kunye ne-supernatants
I-IL yomntu - Ii-ELII ze-ELISA zisetyenziselwa ukulinganisa amanqanaba e-cytokine kwiPlasma kunye neNkcubeko yeSondonaManzi. Ukukwazi ukuhlalutya il - 2 Ukugxininiswa kwezi zinxibo kubalulekile ekuqondeni inkqubo-nkqubo yenkqubo ye-ukhuseleko, ngakumbi kuphando olujolise kwizifo ze-inflamic kunye nongenelelo ngoncedo.
Iinkcukacha ze-FOLOMETICTIC ELISA
● Inyathelo - Ngokwenkqubo yenyathelo
Inkqubo yesiqhelo ye-lelisa i-Elisa ibandakanya uthotho lwamanyathelo aqala ngelungiselelo lesampulu, kulandelwe ngokufakwa ngokufaka ii-antibodies. Emva kokuba izifungo ezininzi zokususa izinto ezingafunekiyo, ukufunyanwa kongezwa. Inyathelo lokugqibela liquka ukongezwa ngokutsha kwe-enzyme ukuvelisa utshintsho lombala olinganiselweyo, ukubonelela ngedatha yobungakanani kwi-IL - Amanqanaba ama-2.
● Ukuqinisekiswa kwiNkcubeko yeSeli
Ukuthambisa i-IL - 2 kwiiseli zenkcubeko yeselula inika ukuqonda kwimveliso ye-cytokine phantsi kweemeko ezahlukeneyo zokuvavanya. Oku kubaluleke kakhulu kwizifundo ukukhangela amajoni omzimba oselula, apho kusenziwa amanqanaba e-cytokine anokunceda ekuqondeni iindlela zokutsala iindlela kunye neziphumo zeselfowuni.
Ukubaluleka kwe-IL Echanekileyo - Imilinganiselo ye-2
● Uphando kunye neziphumo zeklinikhi
I-IL echanekileyo - Imilinganiselo ye-2 iSiseko sobubini kuphando kunye nezicelo zeklinikhi. Kuphando, iyenza ukuba kubekho uphawu lweempendulo zokuhlala ukhuselo, ekwazisa ukuphuculwa kwamajoni kunye nezitofu zokugonya. Ikliniki, il - Amanqanaba ama-2 anokusebenza njengabamamakishi kwizifo okanye impendulo yonyango, asebenzayo kulawulo lwesigulana kunye nonyango.
● Impembelelo kwiSifundo seCytokine kunye nonyango
Ukukwazi ukulinganisa il - 2 Inamachaphaza ama-2 ngokuchanekileyo kwi-cytokini isifundo kunye nonyango. Iququzelela ukuqondwa okunzulu kwenethiwekhi ye-cytokine kunye neendima zabo zempilo nezifo, ekugqibeleni, ukukhokela ukuphuculwa kolawulo lwe-cytosikaine ekujoliswe kulo kunokwenza umsebenzi ofanelekileyo.
Imiceli mngeni kwi-IL - Ukufunyanwa kwe-2
● Imvakalelo kunye nemiba ethile
Omnye wemiceli mngeni ephambili kwi-IL - Ukufunyanwa kwe-2 kuqinisekisa ubuntununtunu kunye nobunye. Umahluko kwi-Anticley Intsebenzo inokukhokelela ekungahambelaniyo, igxininisa ukubaluleka kokukhetha i-Il yomntu othembekileyo - 2 Abathengisi be-TIT eqinisekisa umgangatho weemveliso zabo.
● Ukwahluka kwesandla sesampula
Omnye umngeni obalulekileyo kukungaqondakali okhethiweyo ngexesha lokuphatha isampulu kunye nokulungiselela, okunokuchaphazela ukuchaneka kunye nokuzala kweziphumo. Iiprothokholi ezisemgangathweni kunye noqeqesho zibalulekile ekunciphiseni le micimbi, iqinisekisa ukuba idatha efunyenwe kwi-IL yomntu - Iikhithi ezi-2 zezokuchonga kwaye zithembekile.
Amathemba exesha elizayo e-Il - Iikhilomitha ezi-2
● Ukuvelela kwezobuchwephesha
Ikamva lomntu le-IL - Iikhithi eziKufumanisileyo zithembisa, ngenkqubela phambili kwezobuchwephesha ekhuthaza ubuntununtunu, bhetyelwe ngokutsha, kunye nokusetyenziswa ngokulula. Izinto ezintsha ezinje nge-ASEX ye-ASTAYs kunye nenqaku - Kuvavanyo lokukhathalela kunokwenzeka ukuba landise ukusetyenziswa kwezi kits, ezenza ukuba bafikeleleke kwaye basebenze ngeendlela ezahlukeneyo.
● Usetyenziso olubanzi kunye nezinto ezintsha
Njengoko ukuqonda kwethu i-IL - 2 Biology Basanda, ke ziya kusetyenziswa kwe-IL - I-2 yeeKits ezifunyenweyo. I-NOELS Traperies I-IL - Iindlela ezi-2 zikwindawo ethe tyaba, enokubakho ngokuguqula iindlela zonyango kwizifo zemoto, umhlaza, nangaphaya. Ukutshixwa kwezinto ezintsha ngabantu
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I-Bluekit nguJiangsut Rillgene, elungiselelwe eSuzhou, ligama elikhokelayo ekuphumeleleni i-selfowuni. Ngombuso wayo - I - Indawo - ART - ART - ART I-Hillgene ikhethekileyo kumaqonga okuphuhlisa ukwenziwa kwemveliso ye-nucleic yemveliso ye-nucleic kunye ne-QC yovavanyo lwe-QC, ukuxhasa uphuhliso lwemoto - T, i-TCR - Bazibophelele ukukhawulezisa i-sellound yemveliso yemveliso yeselula,I-BluekitIhambelana nolawulo lomgangatho kwimveliso yeziyobisi. Injongo yabo iyaqhubeka ukukhuthaza inkqubela phambili kwiimveliso zeselula, ezixhamla izigulana kwihlabathi liphela.
IXESHA LOKUQALA: 2024 - I-12 - 09 15: 26:03
