Intshayelelo
Ukukhutshwa kwe-DNA ye-NENN yinkqubo ye-molecular ye-molecular, idlala indima ebalulekileyo kusetyenziswa iintlobo ezahlukeneyo zophando kunye nezicelo zonyango. Ukuphuculwa kweKikhi yeDNA ye-DNAI-s ihamba iguqukele le nkqubo, iyenza ifikeleleke, isebenze kwaye ithembekile. Eli nqaku lidla ngokufika kwi-numing hlacotho ye-DNA ye-DNA, iphonononge iinkqubo ezahlukeneyo zesampulu, iinkqubo zokucoca, imiceli mngeni, uvavanyo lomgangatho, izixhobo zokuthengisa, kunye nezolo. Ugxininiso olukhethekileyo lubekwe kubaluleke kubabalulekile kwiseli yeseli yeseli ye-DNA ye-DNA ye-DNA yeKits. Enoba ungumphandi, ugqirha wezikolo, okanye uchwepheshe welebhu, esi sikhokelo sibanzi siza kusinika ulwazi oluxabisekileyo kubandakanyiweyo kunye nokuhambela phambili kwi-DNA ye-DNA ye-DNA.
Intshayelelo kwiiKits zokucoca
● Amagqabantshintshi ngeekhilomitha zokucoca i-DNA
Izixhobo zokukhutshelwa kwe-DNAIC ze-DNA zibalulekile kwizixhobo ze-moleculatoratries. Ezi kiks zenza lula inkqubo yokukhuphela, iqinisekisa phezulu - I-DNA esemgangathweni ifumaneka kwiindidi ezahlukeneyo zesampulu. Babonelela ngomgaqo omiselwe umgaqo omiselweyo, ukunciphisa ukwahluka kunye nokuphucula ukuzala kwakhona. Ifumaneka kwiifomathi ezininzi, ezi kits ze-kits kwiisayizi ezahlukeneyo zesayizi kunye neemfuno zokufaka.
● Ukubaluleka kwezicelo ezahlukeneyo
I-DNA Ukucoca izixhobo zeKits zibalulekile kuphando lwemfuzo, indlela yokufumana ulwazi, i-forensics, kunye nezifundo zokusingqongileyo. Ukuphanda, banceda ukufundwa kwezinto zemfuza, ukubonakaliswa kwemfuza, kunye nokulungiswa kwe-epigenetic. Kwiseto yeklinikhi, basetyenziselwa ukufumanisa ukuphazamiseka kwemfuza, ukubeka esweni ukusebenza kakuhle unyango, kunye nokuphuhlisa amayeza abucala. Ukuthembeka kunye nokusebenza kakuhle kwezi kits zibaluleke kakhulu ekuveliseni iziphumo ezichanekileyo nezibuyisiweyo.
Iindidi zeesampulu zeDNA
● Iiseli zegazi ezimhlophe
I-DNA ye-gemic inokuthi ikhutshwe ngokufanelekileyo kwiiseli ezimhlophe zegazi kusetyenziswa izixhobo ezizodwa. Oku kuluncedo ngakumbi kuvavanyo lweklinikhi kunye novavanyo lwemfuza, apho iisampulu zegazi ziqokelelwa rhoqo.
● Iiseli zenkcubeko yezixhobo
Iiseli zenkcubeko yezicubu zibonelela ngendawo elawulwayo yokufunda ngezinto zemvelo. Ukukhutshwa kwe-DNA kwezi seli kubalulekile ukuba uphando kwiselula nemolekyuli. Ii-kits zenzelwe iiseli zenkcubeko yezicubu ziqinisekisa isivuno esiphezulu nobunyulu.
● I-ties kunye nezixhobo zezityalo
Ukukhupha i-DNA evela kwizilwanyana kunye nezicubu zezityalo kubeka imiceli mngeni eyodwa ngenxa yobukho babathengisi kunye nezakhiwo ezintsonkothileyo. Iikhilotiko ezikhethekileyo ze-DNA zenzelwe ukuphatha ezi zinto zinzima, ukuqinisekisa ukuba i-Lysis kunye nokuhlanjululwa okusebenzayo.
● Igwele kunye nebacteria
Iiseli zentsholongwane nentsholongwane zineendonga zeseli ezinamandla ezifuna iindlela ezithile ze-Lysis zokukhutshwa kwe-DNA ngokufanelekileyo. I-KITS ilungelelaniswe le microorganisms isebenzisa ubuchwephesha bokuphazamiseka okanye oomatshini wokuphazamiseka ukukhulula - I-DNA esemgangathweni ye-gemic.
Inyathelo - Ngokuphiliso lwenkqubo yokucoca i-DNA
● Ukulungiswa kwesampulu yokuqala
Inyathelo lokuqala kwi-DNA lokucoca kubandakanya ukuqokelela nokulungiselela isampulu. Oku kunokubandakanya ukuqhekezwa kwekhulu, ukuhlamba, kunye noSumonicance, kuxhomekeka kuhlobo lwesampulu. Ukulungiselela kwesampulu efanelekileyo kubalulekile ukukhulisa i-DNA ye-DNA kwaye umgangatho.
● I-Lysis kunye namanqanaba okubopha
Inqanaba le-lysis libandakanya ukwaphula imithambo yeseli kunye nokukhulula i-DNA ye-gemic kwisisombululo. Oku kufezekiswa kusetyenziswa izitoli, i-enzymes, okanye iindlela zoomatshini. Inqanaba lokubopha lilandela, apho i-DNA ibotshelelwe kwisigaba esiqinileyo, njenge-silica, phantsi kweemeko eziphezulu zetyuwa.
● Ukuhlamba kunye nelinge
Amanyathelo okuhlamba asuse ungcoliseko kunye nokungcola, ukuqinisekisa ubunyulu beDNA. Ii-buffers ezininzi zokuhlamba zisetyenziselwa ukuphelisa iiproteni, i-lipids, kunye nezinye iiseti zeselula. Okokugqibela, inyathelo le-esiluthol likhupha iDNA ehlanjululwe kwiSigaba esiqinileyo usebenzisa i-buffer ephantsi okanye amanzi.
Isisombululo - Iindlela ezisekwe kwi-DNA
● Uthelekiswa nezinye iindlela
Isisombululo - Iindlela ezisekwe, ezinje nge-phenol - Ukususwa kwe-chlorofor kunye nokuhambisa ngaphandle, ziindlela zobuchwephesha be-DNA ukucocwa kweDNA. Ngelixa besebenza, basebenza - zibanzi kwaye zifuna iichemicals eziyingozi. Ngokwahlukileyo, i-DNA yokukhupha i-DNA yanamhlanje ibonelela ngokukhuselekileyo, ngokukhawuleza, kunye nomsebenzisi ngakumbi - ezinye iindlela ezizezinye.
● Ngabaninzi izibonelelo zesisombululo - Inkqubo esekwe
Isisombululo - Ukususwa kwe-DNA esekwe kwi-DNA ukubonelela ngeenzuzo ezininzi, kubandakanya isivuno seDNA esiphezulu, ubunyulu, kunye nokuzala kwakhona. Zenzelwe ukunciphisa ungcoliseko kwaye zinokufikelelwa ngokulula kwizicelo eziphakamileyo - Izicelo zokungena. Ngapha koko, azifuni zingozi zekhemikhali, zenza ukuba zikhuseleke kubasebenzi belebhu.
Imiceli mngeni kwi-DNA Ukucocwa
● Imicimbi eqhelekileyo edibene nayo
Ukucocwa kwe-DNA kunokwenziwa yimiceli mngeni, njengesivuno esisezantsi, ungcoliseko, kunye nokuthambisa. I-inhibitors ekhoyo kwisampulu inokuphazamisa izicelo ezisezantsi, kwaye ukuphathwa gadalala kungakhokelela ekuchebeni kwe-DNA.
● Iingcebiso zokulungisa ingxaki
Ukujongana nale miceli mngeni, kubalulekile ukuba ulandele iprotocol yeTti Meticaly. Ukusebenzisa ii-regentnts ezintsha, ukugcina imeko yokusebenza ecocekileyo, kunye nokuqinisekisa ukulungiselela isampulu efanelekileyo kunokuthomalalisa imiba emininzi. Ukongeza, kubandakanya amanyathelo olawulo esemgangathweni, njengokulinganisa ugxininiso kunye nokucoceka, kunokunceda ekusombululeni iingxaki kwangoko.
Izicelo zophando kunye namayeza
● Sebenzisa kuphando lwemfuza
Ukukhutshwa kwe-DNAIC ye-DNA yinto ebaluleke kakhulu kuphando lwemfuza, ukuvumela ukunika izifundo kumsebenzi wemfuza, ummiselo kunye nonxibelelwano. Ixhasa ubuchwephesha njenge-PCR, ukulandelelana, kunye ne-genobing, ebaluleke kakhulu ekuqondeni iindlela zemfuza kunye nokuphuhlisa amacebo amatsha onyango.
● Izicelo kwiKlinikhi yeKlinikhi
Kwisihlomelo seklinikhi, i-rive - I-DNA esemgangathweni i-DNA ibalulekile kwisifo esichanekileyo sosifo, i-progunosis, kunye nokubek'esweni. Izicelo ziquka uvavanyo lwemfuza kwiziphazamiso ezililifa, ukucetywa kwesifo esosulelayo. Ukuthembeka kweekhilotho ze-DNA ekuba kubaluleke kakhulu ekuboneleleni ngolwazi oluchanekileyo nolwaxesha.
Umgangatho kunye nesivuno seDNA ephantsi
● Izinto ezichaphazela umgangatho we-DNA
Izinto ezininzi zinefuthe kumgangatho kunye nesivuno seDNA ecocekileyo, kubandakanya nohlobo lweesampulu, indlela yokukhupha, kunye neenkqubo zokuphatha. Ubukho be-inhibitors, ezinjengeeproteni kunye ne-polysaccharides, inokuchaphazela izicelo ezisezantsi.
● Iindlela zokulinganisa ukucoceka kwe-da kunye nokugxininisa
Ukuvavanya ubunyulu kunye nokugxininiswa kwe-DNA kubalulekile ekuqinisekiseni ukufaneleka kwayo kwizicelo ezisezantsi. Iindlela eziqhelekileyo zibandakanya uhlalutyo lwe-spectrophotometric, enikezela ngenqanaba le-A260 / A20 / A20) lokuvavanya ubunyulu, kunye ne-fluorometric and and averateral ezibuthathaka kwaye ezichanekileyo.
I-Automation kunye nokuhlanjwa okuphezulu kwe-DNA
● Itekhnoloji eyenza iinkqubo ezenzekelayo
I-Automation kwi-DNA Ukucocwa kuye kwathi kubaluleke ngakumbi ekuphatheni imiqulu emikhulu yesampulu kunye nokuphucula ukusebenza kwendlela yokuhamba. Iinkqubo ezenzekelayo, ezinjengamaqonga olwenzi beerobhoti kunye namaqonga okuqinisa okuzenzekelayo, ukulungelelanisa inkqubo, ukunciphisa isiphoso somntu, kwaye sonyuse iziphumo.
● Izibonelelo zeZisombululo eziPhakamileyo
Izisombululo zokucoca i-DNNA yokucoca i-DNA ibalulekile kwizifundo ezinkulu zemfuza, indlela yokufumana isifo seenqwelomoya, kunye ne-bibbanking. Benza ukuba kwabelwe iisampulu ezininzi, ukonga ixesha kunye nezixhobo. Iinkqubo ezenzekelayo zibonelela ngeziphumo ezingaguqukiyo, ukuphucula ukuzala kunye nokuthembeka.
Uhlalutyo lokuthelekisa lweikhithi
● Ukuguqulwa kwentsebenzo yeekhithi ezithandwayo
Kukho iindlela ezininzi zokukhupha i-DNA ye-DNA yokukhuphela i-Kits ezifumanekayo kubavelisi abahlukeneyo, nganye inezinto ezahlukeneyo kunye neempawu zentsebenzo. Izinto ekufuneka ziqwalaselwe xa ukhetha ikhithi ibandakanya isivuno, ukucoceka, ukulungeleka kokusetyenziswa, kunye neendleko. Izifundo zokuthelekisa kunye nophononongo lomsebenzisi lunokubonelela ngengqondo ebalulekileyo ekusebenzeni kweekhithi ezahlukeneyo.
● Ukuphononongwa kwabathengi kunye nezimvo zeengcali
Impendulo yomsebenzisi kunye noPhononongo lweNgeniso zidlala indima ebalulekileyo ekuvavanyeni ukusebenza ngempumelelo kwe-DNA ye-DNA ye-DNA ye-DNE. Uhlolo oluqinisekileyo kunye nokuGqibela kwimithombo edumileyo kunokukhokela abaphandi ekukhetheni ikhiti ilungileyo kwiimfuno zabo. Ukongeza, ingxelo evela kubasebenzisi inceda abavelisi ukuphucula iimveliso zabo kwaye bajongane nayiphi na imicimbi.
Iindlela zexesha elizayo kwiitetho zohlanyazo lweDNA
● Itekhnoloji ekhulayo kwi-DNA yokukhutshwa
Intsimi yokucocwa kwe-DNA iqhubeka ivela, ngetekhnoloji entsha ivela ukujongana nokusikelwa umda okwangoku. Izinto ezintsha ezinjenge-microfluidics, i-nanotechnology, kunye ne-bead ye-bead - Ukukhupha okusekwe kwi-DNA yokucoca i-DNA.
● Uqikelelo lophuhliso lwexesha elizayo ebaleni
Imeko yexesha elizayo kwi-DNA Ukucocwa kugxile ekwenzeni okungaphezulu, ubumbili, kunye nokudityaniswa kobugcisa beemolekyuli zemolekyuli. Ukuphuhliswa kweekhithisi zendalo zonke ezinokuthi zikwazi ukuphatha uluhlu lweentlobo zesampulu kunye neemeko kulindeleke ukuba zandise ukuguquguquka kunye nokungahambi kakuhle kwi-DNA yokukhupha umsebenzi.
I-Bluekit: I-DNA ye-DNA yoovulindlela
Jiangsu Hillgene, phantsi kwegama lophawuI-Bluekit, uzimisele ukuba nomdlali ophambili kwicandelo le-DNA ye-DNA. Ibekwe eSuzhou, kunye neziza ezivelisa i-Shenzhen naseShanghai, kunye nendawo ezayo eNorth Carolina, induli inikezela ngombuso - Imveliso yeBluekit yenzelwe ukuhlangabezana neemfuno ezinqabileyo zeselula zonyango, ukuxhasa uphuhliso lwemoto - T, i-TCR - Ngokuzibophelela kubuchule bobuchule kunye nomgangatho, induli inikezelwe ekuqhubeni intsimi ye-selfowuni kunye nokuphucula iziphumo zesigulana.
Isishwankathelo, ukuhlanjululwa kweDnamic yinkqubo ebaluleke kakhulu kwimolekyuli yemolekyuli, ngokubanzi - Ukuzifaka izicelo ezikhoyo kuphando kunye namayeza. Ukuhambela phambili kwi-DNA yokukhutshelwa kwe-DNA yokukhutshelwa kwe-DNA, kubandakanya le nto ngeBluekit, yenze le nkqubo isebenza ngakumbi, inokufikeleleka, kwaye ifikeleleke. Njengoko itekhnoloji iyaqhubeka ivela, ikamva lokucocwa kwe-DNA libambe amathuba anomdla wokuphucula ukufunyanwa kwesayensi kunye nokuyifumana.
IXESHA LOKUQALA: 2024 - 09 - I-27 14 40:06
