Zeziphi izinto ezibandakanyiweyo kwikhithi ye-BSA?

Intshayelelo kwiI-BSA KITs

I-Bovine Serum Albumin (BSA) ziikhithi ze-BITS zizixhobo ezingenantsingiselo kwiilebhu zebhayoloji ne-biochemical laboratries. Ezi kits zenzelwe ukunceda abaphandi kunye neengcali zelebhu ekwenzeni izicelo ezahlukeneyo zelebhu, kubandakanya ukudityaniswa kweProtein, i-enzyme - i-ESAROOKESEALTEATE Ukuqonda izinto ezibandakanyiweyo kwikhithi ye-BSA kubalulekile ekuphunyezisweni okufanelekileyo kunye nokufezekisa iziphumo ezichanekileyo.

Indima ye-BSA kwiimvavanyo

Ukubaluleka kwe-BSA

I-BSA isetyenziswa ngokubanzi njengomgangatho kwiproteyini. Isebenza njengearhente yokuzinzisa i-enzymes kwaye njengophawu lokumakisha. Ukuhamba kweyona nto iphambili kwenza ukuba ibe yinto eqhelekileyo kwi-assos kunye ne-diviynostics.

I-BSA ye-ASTAYS

KwiProtein Assays, i-BSA ihlala isetyenziswa njengesalathiso sokuthelekisa ukugxininisa kweprotein. I-AIDS kwi-ACTISHICRARD, uqinisekisa ukuba iimvavanyo zivelisa iziphumo ezinokuthenjwa nezibuyisiweyo.

Izinto zekhithi ye-BSA eqhelekileyo

Izinto eziphambili

• Umgangatho we-BSA:Icandelo eliphambili lekhithi, elisetyenziselwa ukwenza i-curves ezisemgangathweni.
• Isisombululo se-Buffer:Kubalulekile ukugcina i-PH kunye nokuzinza kwiisampuli.

Icandelo elongezelelweyo

• Imibhobho:Isetyenziselwe umlinganiso ochanekileyo kunye nokudluliselwa kotywala.
• Uvavanyo lweeTubs:Ineesampulu kunye nokuhlaziywa ngokuphendula.

Isisombululo se-Buffer kwi-BSA's Kits

Indima ye-Buffers

I-Buffers idlala indima ebalulekileyo ekugcineni amanqanaba e-PH eyimfuneko yokuthatha inxaxheba ukuba yenzeka ngokugqibeleleyo. Kwiikhithi ze-BSA, ii-Buffers ziqinisekisa ukuba i-BSA kunye nezinye izinto zokuhlala zihlala zizinzile kuyo yonke inkqubo yovavanyo.

Ii-buffers eziqhelekileyo

Ii-Buffers eziqhelekileyo zibandakanya i-phosphate - i-pisred baline (ii-PBs) kunye ne-tris buffer, ilungiselelwe i-Tris Buffer ukuba ilungele iimfuno ezithile kunye neemeko.

Iityhubhu zovavanyo kunye nemibhobho

UKUSETYENZISELWA KWEEMPAWU ZOKUFUNDA

Iityhubhu zovavanyo zisetyenziselwa umxube wokuphendula kwaye zihlala ziphawulwa ngemilinganiselo yevolumu yemilinganiselo engamanzi echanekileyo. Uyilo lwabo lukwaqinisekisa ukudityaniswa ngokuchanekileyo kwee-regenta.

Ukuchaneka kombhobho

Imibhobho ibalulekile kwimozulu echanekileyo kunye nokususwa kolwelo. Ukuchaneka kwabo kubalulekile ekugcineni ukuthembeka kweziphumo zovavanyo.

Iirejista kunye nokusetyenziswa kwazo

Iirejista eziqhelekileyo

Iirejista ezinje ngeedayisi okanye i-chromogenic upecrates zihlala zibandakanyiwe ekuboneni iziphumo zeziphumo. Ezi regentangs zinxibelelana ne-BSA, ukuvelisa utshintsho lombala oluhambelana noxinzelelo lweprotein.

Ukuphathwa ngokutsha

Ukuphatha ngokufanelekileyo nokugcinwa kweerejimitha kubalulekile ukuqinisekisa ukusebenza kwazo. Izinto ezifana nemvakalelo yokukhanya okanye uzinzo lobushushu kufuneka luqwalaselwe.

Isikhokelo soMyalelo kunye noMyalelo

Ukubaluleka kwencwadana yemigaqo

Incwadana yemiyalelo idla ngokufakwa kwiikhithi ze-BSA ukukhokela abasebenzisi ngokuseta olingeneyo, ukuqinisekisa ukuba onke amanyathelo ayalandelwa ngokuchanekileyo ukuba avelise iziphumo ezifanelekileyo.

Inyathelo - Ngeenkqubo zamanyathelo

Le ncwadana idla ngokubonelela ngenyathelo - Ngeenkqubo zamanyathelo, iingcebiso zokulungisa ingxaki, kunye neenkcazo zemigaqo yesayensi phantsi kovavanyo ngalunye.

Izinto zokugcina kunye nokulondolozwa

Izenzo ezifanelekileyo zokugcina

Iikhithi ze-BSA zihlala zibandakanya imiyalelo ethile yokugcina, enokubandakanya ukufunyanwa okanye ukukhuselwa kukukhanya, ukugcina ukuthembeka kwamacandelo eekhiti.

Iingcebiso zolondolozo

Ukulungiswa rhoqo kwezixhobo, ezinjengombhobho wokulinganisa, kubalulekile ukuphepha iimpazamo zokulinganisa kwaye uqiniseke ukuthembeka kweziphumo zovavanyo.

Ukwahluka kwezinto ze-BSA

Iindidi ze-BSA ze-BSA

Iikhithi ze-BSA ziza ngeendlela ezahlukeneyo, zenzelwe ukuba izicelo ezithile, ezinjengeeprotein ezisemgangathweni, ukunyanzeliswa kwenkqubo, okanye izongezo zeNkcubeko. Ezi kokit zingahluka ngokusekwe kuxinzelelo lwe-BSA kunye ne-regents ebandakanyiweyo.

Ukukhetha ikhiti elungileyo

Ukukhetha i-BSA efanelekileyo ye-BSA ixhomekeke kwisicelo esicetywayo kunye neemfuno ezithile zovavanyo, ezinjengokungalunganga kunye nokuchaneka.

ISIQINISEKISO: Ukukhetha i-BSA ye-BSA ye-BSA

Ukukhetha ikhithi ye-BSA echanekileyo kubalulekile kwimpumelelo yovavanyo lwakho. Qwalasela izicelo ezithile kunye neemfuno ezinje ngeentlobo ze-buffer, ukuphinda ubuhle, kunye nokugxininiswa komgangatho. Ukusebenzisana nomvelisi onokuthenjwa, umboneleli, okanye umzi-mveliso ukuqinisekisa umgangatho kunye nokungaguquguquki kweekhilomitha zakho ze-BSA.

I-Bluekit ibonelela ngezisombululo

I-Bluekit, umthengisi okhokelayo kunye nomenzi kwicandelo le-biochemical, inika izisombululo ze-BSA ezibanzi ze-BSA zilungelelaniswe iimfuno ezahlukeneyo zalebhu. I-KITS zethu zibandakanya imigangatho ephezulu ye-BSA, iZisombululo eziPhethekileyo, eziPhelekileyo eziPhelekileyo, kunye nazo zonke ii-regenti eziyimfuneko, ukuqinisekisa ukuchaneka kunye nokuzala kwizicelo ezahlukeneyo. Iqabane elineBluekit yoQinisekiso loMgangatho, inkxaso yezobugcisa, kunye nengcali engafakwanga, nokuba ihlawulwe ngokuthe ngqo kumzi-mveliso wethu okanye ngabathengisi abagunyazisiweyo. Khetha iBluekit ukonyusa ukuchaneka kunye nokusebenza kakuhle kovavanyo lwakho lwelebhu.