Intshayelelo
● Ukubaluleka kwe-DNA ye-DNA ye-genomic
Kwindawo ye-molecular biology, ukukhutshwa kwe-DNA ye-gemic yinkqubo esisiseko ebeka isiseko sezicelo, ukusuka kuphando lokuxilongwa kweklinikhi kunye namayeza abucala. Inkqubo yokukhupha i-DNA ye-DNA ibandakanya ukwahlula iDNA yokuhlalutya kunye nokusebenzisa izixhobo zemvelo kwizifo zemfuza, i-biology yendalo, kunye nokuhambela kwebhayoloji. Ngemfuno ye-burgeoning yeZifundo zeWemic, indima yokuthenjwaKikhi yeDNA ye-DNAayikaze ibaluleke ngakumbi. Ezi kits zisingisa inkqubo yokukhutshwa, ukuqinisekisa isivuno esiphezulu kunye nokucoceka kweDNA, kubalulekile kwizicelo ezisezantsi.
Iindlela zokuqokelela isampula
● Iindidi zeesampulu ze-DNA yokukhutshwa
Ingqibelelo ye-DNA ye-Wemic iqala ngokuqokelelwa kweesampulu eziphakamileyo. Imithombo eqhelekileyo iquka igazi, izicubu, amathe, kunye ne-tycal swabs. Uhlobo lwesampulu lubonelela ngeengenelo ezizodwa; Umzekelo, iisampulu zegazi zityebile kwi-DNA kodwa zifuna ukuphatha ngononophelo, ngelixa i-baliva kunye ne-wiccal swabs zibonelela ngeenketho ezingezizo Ukukhetha isampulu efanelekileyo kubalulekile, ngakumbi xa kusetyenziswa iKithi yoTyhilo lweDemic yeDNA ye-DNA yeDNA, enokupheliswa kweentlobo zeseli ezithile, ukuqinisekisa isivuno esikhulu nobunyulu.
● Iindlela ezilungileyo zokuphatha isampula
Ukuqinisekisa ukuthembeka kwesampulu kubalulekile ekuqinisekiseni i-DNA ephumeleleyo. Iisampulu kufuneka ziqokelelwe kungcoliseko - Indawo yasimahla, igcinwe kwiqondo lobushushu elifanelekileyo, kwaye liphathwe ngononophelo ukuthintela ukonakaliswa. Eli nyathelo libalulekile ukuba ujongene neesampulu ezitsha okanye ezigcinwe ixesha elide, njengoko nayiphi na i-Debreadesing inokulalanisa isivuno sokugqibela se-DNA kunye nomgangatho, echaphazela uvavanyo.
Iindlela zeLysis
● Iikhemi ze-ly
Inyathelo leseli Lysis liyinto ebaluleke kakhulu ekukhulula i-DNA ye-gemickec evela kwimibutho yeselula. I-Lylisis yekhemikhali, ebandakanya ukucocima kunye nee-enzymes, uqhekeza kancinci ii-membrane zeseli kunye neeprotein. Iindlela zomzimba-ezinjengokuphazamiseka koomatshini kunye nokuzihlaziya - zinamandla ngakumbi kwaye zinokusetyenziselwa iisampulu ezinamandla. Ukhetho lwendlela luhlala luxhomekeke kuhlobo lwesampulu kunye neemfuno ezithile zeKit ye-DNA ye-DNA ye-DNA yokuSebenzisa. Abavelisi abayithethweni kits zokuphucula ukusebenza kakuhle kwe-lysis, ukulinganisa phakathi kokulula kunye nokusebenza kakuhle.
● Ukubaluleka kwe-Lysis kwi-DNA Khupha
I-Lysis efanelekileyo yokuqinisekisa ukuba izinto zeselula ziqhekezwe ngokufanelekileyo, ziququzelele ukukhutshwa kweDNA ye-Iscoung kwisisombululo. Iphucukileyo ngokufanelekileyo inciphisa ukucheba kweDNA kunye nongcoliseko kwiiproteni kunye nezilids, ezibaluleke kakhulu kwizicelo ezifana nonyango lweseli, apho ingqibelelo yeWemidic ibaluleke kakhulu.
Ukususwa kweeproteni kunye nokungcola
● Indima yokuzithoba ezihlanjululwe
Thumela - Lysis, ukususwa kweeproteni kunye nezinye izinto ezingcolileyo kubalulekile. Izinyibilikiswa njenge-phenol - i-chloroform ngokwesiko eqeshwe kwi-subture kunye neeprotein ezahlukeneyo ezivela kwii-acic zenyukliya. I-genomic ye-genomic ye-DNA yanamhlanje ihlala isebenzisa i-silica - ii-membranes ezisekwe okanye zezazi, ezibopha iDNA ngokukhethekileyo ngelixa ivumela ukungcola ukuba ihlanjwe. Eli nyathelo libalulekile ekufumaneni i-DNA ecocekileyo efanelekileyo ukuze ufumane izicelo ezichanekileyo nezinobuthathaka.
● I-ntenterifugagugagetion ichaziwe
Ikhulu linyathelo eliqhelekileyo lokwahlula i-DNA kwiPost yasemva kwezinto - Lysis. Ngokusebenzisa amandla amakhulu e-centrifugal, ubushushu obunzima baseselula kwaye iiproteni zipeyisiwe, ngelixa iDNA ihlala ikwi-supernatant. Eli nyathelo lihlala liphindaphindwa ngokuhamba ngenyathelo lokucoca ukuphucula ubunyulu kunye nokugxininiswa kweDNA ekhutshiweyo.
Inkqubo ye-DNA ye-DNA
● Ukusetyenziswa kwe-Iyopropanol kunye ne-ethanol
I-PARPICT yindlela yeklasi yokufumana kwakhona i-DNA kwisisombululo, ixhaphazwe phantse kuzo zonke iindawo zokukhupha i-DNA ye-CNNICK. I-alcolols i-isprels njenge-Iyopropanol kunye ne-ethanol yenza ukuba i-DNA isebenze ngokunciphisa isolula. Nje ukuba idlule, ii-DNA ii-phuls ezibonakalayo okanye iifiliments, zibonelela nge-cue ebonakalayo yokukhupha ngempumelelo.
● Ukuboniswa kwe-DNA Filaments
Ubukho be-DNA Filaments uqinisekisa ukutshangozelwa kwaye kusebenza njengempawu zokuqala zempumelelo. Eli nyathelo, nangona sibonakala sisisiseko, sibalulekile ukuze sizithembe kwinkqubo yokukhutshwa, ukuqinisekisa ukuba i-DNA eyaneleyo inexesha lohlalutyo olulandelayo.
Ukuhlamba i-DNA ePhakamileyo
● Ukubaluleka kokuhlamba i-ethanol
Ukuhlamba i-DNA Pellets nge-ethanol isebenzela ukususa iityiwa kunye nokungcola. Eli nyathelo, nangona kubonakala ngathi lisebenza, kubalulekile ukuthintela ungcoliseko olunokuchaphazela iinkqubo ezinamanzi. Ukuhlamba i-ethanol kuchazwe ngokufanelekileyo kwincwadana yabasebenzisi yi-DNA ye-DNA yokukhuthaza i-DNA yokuqinisekisa ukuba abasebenzisi bafezekise iziphumo ezilungileyo.
● Ukuqinisekisa ukucoceka kweDNA
Ukucoceka kubaluleke njengesivuno kwi-DNA yokukhutshwa. Ukungcola okungasasebenziyo kunokuthintela ukwenziwa kwe-enzymatic kwizicelo ze-dowwwings ezinjenge-PCR kunye nokulandelelana. Ababoneleli be-DNA ye-DNA yokuKhokelela kwe-NENIN igxininisa ubunyulu, bonisa iiprothokholi kunye nezixhobo eziqinisekisa ukuba ungcoliseko luhlanjwe ngokupheleleyo.
Ukunyibilika kunye nokulungiselela i-DNA
● Ukukhetha i-buffer efanelekileyo
Nje ukuba i-DNA ihlanjululwe, ichithwe kwi-buffer efanelekileyo, phantse i-buffer okanye amanzi anqabileyo. Ukhetho lwe-buffer lunokuchaphazela uzinzo kunye nentembeko ye-DNA, ichaphazele ixesha elide - ithuba lokusebenza. Iifektri ze-DENIC ze-DENA yokuKhokelela kwi-CNNAS
● Ukulungiselela i-DNA yovavanyo
Ulungiselelo olufanelekileyo lweDNA lubalulekile kwimpumelelo yovavanyo, ukuqinisekisa ukuhambelana neendlela zohlalutyo ezinjenge-QPCR, elandelayo - Ukulandelelana, nokubetheka. Iindlela zokulungiselela ngokugqibeleleyo zandisa ukuthembeka kwe-DNA kunye nokusebenza kwezi zicelo.
Ulawulo loMgangatho weDNA ekhutshiweyo
● Iindlela zobuchule bokuvavanya umgangatho we-DNA
Emva kokukhutshwa, ukuvavanya umgangatho we-DNA kubalulekile. I-Spectraphotometry yindlela eyaziwayo, yokulinganisa nge-260 NM kwaye ibonelela ngolwazi malunga nokugxila kweDNA kunye nobunyulu. Ukongeza, i-gel electrophoreris ivumela ukubonakaliswa kwe-DNA ingqibelelo, ifumana nayiphi na idayisi.
● Ukuqonda ukufundwa kwe-spectrophotometer
Ukufundwa kwe-Spectraphotometer Funds kunika ukuqonda kubunyulu kunye nokucoceka kweDNA. Umlinganiselo we-260/2000 usondele kwi-1.8 ibonisa i-DNA ecocekileyo, ngelixa ukuphambuka ibonisa ungcoliseko. Oku kufundwa kubaluleke kakhulu ekuqinisekiseni ukuba i-DNA ilungele izicelo ezinzulu ezisezantsi.
Imiceli mngeni kwi-DNA
● Imiba eqhelekileyo kunye nokulungiswa kwengxaki
Ngaphandle kwenkqubela phambili, imiceli mngeni kwi-DNA yokukhupha iyaqhubeka, kubandakanya isivuno esisezantsi, ungcoliseko, kunye nokonakaliswa. Ukuqonda le micimbi kunye nokulandela izikhokelo zokucombulula iingxaki - zihlala zinikezelwa ngabavelisi-zibalulekile ekukhuthazeni ngempumelelo.
● Ukwahluka kweentlobo ezahlukeneyo zesampulu
Iindidi ezahlukeneyo zesampulu ibonakalisa imiceli mingeni eyodwa kwi-DNA yokukhutshwa, njengoMxholo we-DNA oguqukayo kunye nobukho be-inhibitors. I-CKINE CKIC ye-DNA yokuKhokelela kwabaThengi beeKit
Inkqubela phambili kwitekhnoloji yokukhupha i-DNA
● Uvelwano kwiikhithi zeKits
Ukuhambela phambili okuqhubekayo kwi-DNA ye-DNA ye-DNA yeKits yenze lula inkqubo, ukuphucula ukusebenza kakuhle, isantya, kunye nokuthembeka. Ezi zicelo zibandakanya itekhnoloji ye-automation, ye-bead bead, kunye nokudityaniswa neerobhothi zoocelo zokufaka izinto eziphezulu, zibonisa iimfuno zokuvela zophando lweZomic.
● Iimeko zexesha elizayo kwiNzululwazi yezeMibidic
NjengeeSayensi ze-Enemic kuhambela phambili, imfuno ye-DNA ephezulu ye-DNA iya kukhula. Iindlela zexesha elizayo zibandakanya ezinye ze-ECO - Iindlela zokukhupha ezinobuhlobo, ukungenisa ngakumbi, kunye nokudityaniswa kokukhutshwa kweetekhnoloji zohlalutyo ezilandelayo, zisiphalaze ngendlela yokuhlalutya okusebenzayo kunye nezibanzi.
I-Bluekit: Nguvulindlela kwi-Wemic kunye neZisombululo zeSelfowuni
Jiangsu Hillgene, phantsi kophawuI-Bluekit, zimi ngaphambili kwi-gemic kunye neselula zonyango. I-Suzhou esenziwe eSuzhou enamaziko ongezelelweyo kwiShenzhen naseShanghai, kunye nendawo ezayo eNorth Carolina, induli inda ifikelela kwiHlabathi liphela. I-Bluekit®Iimveliso zibonelela ngezisombululo ezibanzi zolawulo oluphawuleleyo kunyango lweseli, ukuxhasa ukuphuculwa konyango oluphambili njengemoto - T kunye ne-TCR - T. Ngamaqonga azinikeleyo kwimveliso ye-Incleic Acid kunye ne-QC, iBluekit iguqukele kubume bendlela yeselula, ukuzisa iimveliso zenguqu ukuthengiseka ngokukhawuleza nangokufanelekileyo.
IXESHA LOKUQALA: 2024 - I-12 - 05 15:07:02
