Zithini izibonelelo zokusebenzisa i-BCA kit?

Intshayelelo kwi-BCA yePREDIN

Iprotein ye-BCA (BicincINninic Acid) Iprotein Assay yindlela eyiyo yovavanyo kwindawo yophando lwe-biochemical kunye nonyango lweseli. Iququzelela ubungakanani be-protein yeprotheni kwisampulu. Le ndlela isebenza kumgaqo-nkqubo weprotheyini - Iphephe yobhedu, ilandelwe ngumbono wemidlalo ye-lemidlalo yokunciphisa ubhedu. Ukuthandwa kwayo kufana nokwenza isicelo kwayo kwezinto ezisetyenzisiweyo kuzo zonke iisampulu zeprotein ezininzi, kubandakanya nezo zongezelelo ezifana noongezelelo, zenza ukuba kuthathe ukhetho kubo bobabini abavelisi kunye nabathengisi basendle.

Ukuhambelana nezitolitha

Ukuphatha iisampulu ezintsonkothileyo

Esona sibonelelo esibonakalayo se-BCA yeproteni yeprotein kukuhambelana nezicoci. Ngokungafaniyo nezinye iindlela ezininzi zeproteyini, i-BCE Assay ingayifumana ukuya kuthi ga kwi-5% ye-surfacants kwisampulu. Eli nqaku liyingenelo ikakhulu kubaphandi kunye nabavelisi abajongana neesampulu ezintsonkothileyo, apho isiciko sihlala sisetyenziswa khona ukuze soyise iiproteri. Kwizicelo zonyango, oku kuyaqinisekisa ukuba ukudityaniswa kweProtein kuhlala kuchanekile nangona kukho izinto eziphazamisayo.

Ukusebenza kwiimeko ezinzima

I-BCA DOSY ibonelela ngeziphumo ezinokuthenjwa nangeemeko zokuvavanywa. Ukomelela kwayo ngokuchasene nolwahluka-hlukeneyo kubume beprotheni, njengo-Amino Acid ulandelelwano kunye nemixokelelwane yecala, kunciphisa iprotein - Ukwahluka kwe-protein. Oku kufakwa okubonakalayo kwabaxhasi abafuna iziphumo ezingaguqukiyo nezibuyisiweyo ukuze zihlale ziphezulu - ezisemgangathweni kwiProtein Assay.

Ukuncitshiswa kwexesha lokuseta

Ukusebenza kakuhle kwe-dilution - Imigangatho yasimahla

Ukwaziswa kwe-dilution - Imigangatho yeproteni yasimahla kwi-BCA ASTAY Kit inciphisa ixesha lokuseta nge-Assay ukuya kuthi ga kwi-80%. Le migangatho iqalisiweyo yenzelwe ukudityaniswa kwempungutye enemibhobho ye-Multikhannel, ivumela abaphandi kunye nabavelisi bakhe ukuba bakhuphile inkqubo ye-Assay ngaphandle kokusebenza ngokuchanekileyo. Kwizicelo zonyango, apho ixesha - Uvavanyo oluqhelekileyo luqhelekile, oku ukusebenza kakuhle kubalulekile.

Imowudi yokusebenza yenziwe lula

Oku kulungelelaniswa kunciphisa isidingo samanyathelo okurhawula amaphepha-mvume, angelilo kuphela emva kwe-Pemouming kodwa ethambekele kwiimpazamo. Ngokuphelisa la manyathelo, i-BCA ye-BCA iququzelela ukutshintsha ngokukhawuleza, ukuvumela abathengisi ukuba bahlangabezane neeshedyuli zokuhambisa ngokufanelekileyo kunye nokuphucula imveliso kwizicwangciso zelebhu.

I-protherity ephuculweyo

Umlinganiso ochanekileyo kwiindidi zeprotheyini

Iprotein ye-BCA ibonisa impendulo efanayo ye-protein yeeproteni xa kuthelekiswa nedayi - Ukubopha i-Assays ngendlela ye-bradford. Oku kufanye kokufezekiswa ngokuphendula ngokulinganayo kwinani leebhondi ze-peptide, ukunciphisa ubuntununtunu bokuziva kwi-Assay kwimahluko kwizakhiwo zeproteyini. Abavelisi ababandakanyeka ekuveliseni iindlela zeseli, oku kuqinisekisa ukulinganiselwa ngokuchanekileyo kwifomu yeprotein eyahlukeneyo, ukonwabisa ukuzithemba kwiziphumo ze-asson.

Ukuphuculwa kwe-Assay

Ngokubonelela ngeziphumo ezingaguqukiyo nokuba kukodwa, i-BCA DOSAY ibaluleke kakhulu kubathengisi abafuna ukubanjwa abaxhasi abafuna ukubanjwa kwe-FOCIC yoLawulo lweMigangatho kunye neMveliso. Oku kuhambelana nokuguqulwa kwedatha ethembekileyo ngakumbi, kubaluleke kakhulu ekuqhubeleni phambili kuphando kunyango lweseli kunye nemimandla enxulumene noko.

Imvakalelo kunye nodidi lokufumanisa

Ubuntu obuphezulu bokuxinana okuphezulu

I-BCE Assay ibonelela ngenqanaba eliphezulu lemvakalelo, ekwazi ukufumana uxinzelelo lweprotheni iphantsi njenge-0.5 μg / ml, kwaye igcina umgca kwi-1.5 mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / mg / ml. Olu luhlu olubanzi olunamandla lwenza lube lw ngokufanelekileyo kwizicelo ezahlukeneyo, ukusuka kuphando olusisiseko kuphuhliso lonyango. Imvakalelo enjalo ibalulekile xa ujongene nobungakanani bemizuzu yeeproteni kwiseli yonyango kunye nophando lwekhemesti.

I-paramitha ebonakalayo

Ukuchongwa kwemifanekiso ye-lemidlalo kwi-562 nm kuqinisekisa ukulahleka komqondiso omncinci (ngaphantsi kwe-10%), ukuvumela abavelisi kunye nabathengisi ukufezekisa inani elichanekileyo. Oku kuchaneka kubalulekile ekuphuhliseni iimveliso zonyango olusebenzayo, apho uxinzelelo lweprotheyini luthi luchaphazela ngokuthe ngqo ukusebenza kakuhle.

Ukusetyenziswa ngokulula kwiinkqubo ze-Assay

IINKQUBO ZOKUGQIBELA

Iinkqubo ze-BCA Ilungile - Sebenzisa i-Regegents kunye neProtokholi ethe tye kunciphisa iimpazamo ezinokubakho, kokufezekisa abaphulaphuli ababanzi, kubandakanya abavelisi beendlela zeProtein.

Umsebenzisi - uyilo olunobuhlobo

Yenzelwe ngokukhululekileyo ukuba isetyenziswe engqondweni, i-BCA ye-ossey inciphise imiqobo yobugcisa, ukuqhuba uqeqesho olulula kunye nokwamkelwa ngokukhawuleza ngaphakathi kwelebhu. Oku kufikeleleka kunceda ukulungelelanisa imisebenzi yabathengisi abafuna ukugcina igalelo eliphezulu ngaphandle kokulala ngokulungisa.

Ukuchaneka kunye nokungqinelana

Iziphumo ezinokuthenjwa kuzo zonke iimvavanyo

I-BCA ASASY ihambisa iziphumo ezinokuthenjwa, zibonakaliswa yiproteyini ephantsi - I-I. Oku kungqinelana kubalulekile kuvavanyo oludinga imilinganiselo ephindaphindiweyo ngokuhamba kwexesha, njengaleyo kunyango lweseli, apho iprotein yeprotein ichanekile ngenxa yokuzala kunye nokuqinisekiswa kweziphumo.

Ukubekwa emgangathweni kunye nokulinganisa

Ukubekwa emgangathweni ngokuchasene ne-BSA ecocekileyo evela kwiZiko leSizwe leMigangatho yeMigangatho kunye neTekhnoloji (i-Nist) iqinisekisa ukuba i-BCA ASESS ibonelela nge-curves echanekileyo nebuyisiweyo. Olu luhlu lubalulekile kubavelisi kunye nabathengisi bejolise ekunikezeleni okuphezulu - Umgangatho, iimveliso ezenziwe ngomgangatho.

Imiba yezobugcisa ye-BCA yendlela

Umatshini we-biochemical

Indlela ye-BCA isekwe kukuncitshiswa kwe-cu²⁺ukuya kwi-cu⁺Ngeeproteni kwi-alkaline ephakathi, elandelwa kukusekwa kwe-floples enderple kunye ne-BCA, esebenza njengesalathiso semibala. Esi sibini senyathelo sinika isiseko esinokuthenjwa seProtein, esebenzayo kwiimeko ezahlukeneyo zophando kunye neemeko zemveliso, kubandakanya ukukhula kwemveliso yeseli.

Izibonelelo ngaphezu kwezinye izinto

Xa kuthelekiswa neendlela ezifana neBradford Assay, indlela ye-BCA ibonelela ngeendlela zokusebenza ngenxa yokuhambelana ngokubanzi nemisebenzi ephazamisayo kunye nokusebenza okungaguqukiyo kwiintlobo zeprotein ezahlukeneyo. Oku kwenza ukuba kuthathe ukhetho olukhethiweyo kubavelisi kunye nabathengisi abafuna izisombululo zeproteyishini eziguquguqukayo nezinokuthenjwa.

Ukuthelekisa kunye neBradford Assay

Umahluko ophambili kwindlela

Ngelixa zombini i-BCA kunye neBradford Assay zisetyenziswa ngokubanzi kwi-protein yeprotein, zahluke ngokusisiseko kwiindlela zabo. I-Bradford Arsay ixhomekeke kwidayisi - Ukubopha iprotheyini, nto leyo enokuba ngaphantsi ihambelana kuzo zonke iintlobo zeprotein ezahlukeneyo. Ngokwahlukileyo, i-BCA ye-BCAY ye-Peptide Conning - Ukufunyanwa okusekwe kunika iyunifomu enkulu kunye nokuthembeka, kuluncedo kakhulu kubavelisi kunye nabathengisi bagxile kwiseli enonyango kunye namacandelo anxulumene noko.

IZIBONELELO ZE-BCA SEAY

I-BCA ye-BCA ye-ossay kunye nezicoci, kuluhlu olubanzi olunamandla, kunye nendlela yokuhamba-msebenzi eyenziwe lula zinika umda kwizicelo ezifuna ezi zibonelelo zikhethekileyo. Ukuhamba kwayo ngokweendlela yayo kwenza ukuba kube lufaneleke uluhlu olubanzi lweeseto zovavanyo, into ebaluleke kakhulu kubathengisi ababonelela ngeemveliso zeemfuno ezahlukeneyo zophando.

Ukuqukumbela kunye neengcebiso

Kutheni i-BCA ithandwayo

Iproteni ye-BCA yeproteni imele ukwenziwa ngokungqinelana nezicoci, incitshisiweyo yokuseta ixesha, kunye nokuchaneka kweprofayili, kunye nokuchaneka. Ezi mpawu zikwenza kufanelekile ukuba zikwazi ukwenziwa kwezicelo kunyango lweseli, ukuhambisa iziphumo ezinokuthenjwa nezingaguqukiyo. Abavelisi kunye nabathengisi, i-BCA DOSAy ibonelela ngendlela enokuthenjwa yokuqinisekisa ukuba phezulu - Iprotein esemgangathweni, ibalulekile ekugcineni imigangatho yeshishini kunye nokuxhasa uphuhliso lwemveliso.

I-Bluekit ibonelela ngezisombululo

I-Bluekit isebenza ngokunikezela ngezisombululo eziphuculweyo zeProtein ye-BCA iproteyini, ilungiselelwe ukuhlangabezana neemfuno ezithile zabaphandi, abavelisi, kunye nabathengisi. Iikhikiko zethu zenzelwe ukuba zenziwe umsebenzi ophezulu, zibonelela ngokuhambelana neproteyini yeproteyini eqinisekileyo yezicelo. I-Bluekit, unokuphucula ukusebenza kwenu yokuhamba komsebenzi kunye nokuchaneka, ukuqinisekisa ukuba uhlangabezana neyona migangatho iphezulu kuPhicoqwa loNyango loNyango loNyango loLuhlulo lweSeli noPhuhliso lweMveliso. Vumela iBluekit njengelinye iqabane lakho elithembekileyo kuzo zonke iimfuno zakho zeprotein.