Intshayelelo yokufumana inombolo yenombolo ye-gene
Kunaphakade - Ubume bendawo eguqukayo bophando lwemfuzo, iinombolo zokuphucula iinombolo ziyilitye lesiseko kuzo zombini izicelo zokuxilongwa kunye nonyango. Ukubaluleka kweNdawo yeNombolo yeTene yeTene Phakathi kweemfuza ezininzi zomdla, i-RCL Gene kunye ne-vsvg gene imele iindima zabo kwiinkqubo zeselula kunye nobunjineli bentsholongwane, ngokwahlukeneyo. Eli nqaku lidla ubomi behlabathi lokufunyanwa kwenombolo ye-gene, kugxilwe kwizinto ezintsha njengeI-Gene Copikhi yeXeshakunye neseli yefowuni ye-Tene yeTene yeTene
Itekhnoloji yokuqala yophando ngohlobo lwemfuza
● Indima ye-QPCR kwiGene
Ubungakanani be-Polymerase Chain Reance (QPRCR) ivele njengesixhobo esingasebenziyo kwinani leenombolo zekopi yemfuza. Obu buchule buvumela ukwahlukana ngokuthe ngqo kunye nokudityaniswa kolandelelwano lwe-DNA, kuyenza ilungele ukufumana i-CNVs. Kwimeko yonyango lwemfuza, apho ukuhanjiswa ngokuchanekileyo kunye nembonakaliso ye-gerapeutic genes, i-QPCR - Iindlela ezisekwe kwi-Precinoud Precinous ngokuchanekileyo nangokunyaniseka.
● Unnovauls kwi-lentiviv vector vector
Ukufika kwezinto ze-lentiviviaral kuneendlela ezifanelekileyo zokuhambisa izinto zemfuza kwiiseli ekujoliswe kuzo. Ezi veries, zihlala zixhotywe nge-vsvg gene, zigcwele injineli yokutshintsha ukusebenza kunye nokuzinza. Umdibaniso we-New Seve yeKhenketho ye-Veme deckie kwimveliso ye-Lentiviviral iqinisekisa ukuba amachaphaza ene gene alawulwa ngokulandelelana, eqhuba iziphumo zonyango.
Amagqabantshintshi nge-RCL (VSVG) I-Tene yeTee
● Iimpawu kunye nezibonelelo zeI-BluekitImveliso
I-RCL (VSVG) ikopi yenombolo ye-Gee esuka kwi-Bluekit imele ukuqhubela phambili okukhulu kwiziqulatho zemvelo. Yenzelwe ukuchaneka, ibonelela ngokufunyanwa ngokukhawuleza kwaye kuchanekile kulungiswe amanani ikopi yemfuza, ukhusele kuzo zombini iimfuno kunye neemfuno zekliniki. Iprotocol yayo elungelelanisiweyo kunye nomsebenzisi - Uyilo olunobuhlobo lwenza isixhobo esibalulekileyo kwiilebhu zehlabathi.
● Izinto eziphambili kunye nokusebenza
Kwintliziyo yemveliso yeBluekit yiseti yalo ebanzi yokuphinda isebenze kunye nolawulo, ukuqinisekisa ukusebenza ngokomoya. Ikhithi ibandakanya ijika eliqhelekileyo eliqhelekileyo, livumela abaphandi ukuba bafumane ubungakanani obuchanekileyo kunye nomzamo omncinci. Njengomthengisi ophambili we-phene ye-tem ye-ten ye-tick, iBluekit igxininisa umgangatho kunye nokuthembeka kwiminikelo yayo yemveliso.
I-Fluorescent Probe kunye neendlela ezininzi ze-PLUE
● Ukucaciswa kweendlela zeprojeki ze-fluorescent
Iindlela zobuchule ze-Fluorescent ziba ngumgangatho wegolide kwi-gene. Ngokusebenzisa incwadana enemifanekiso echaza i-Fluorescence kwi-HNA ekujolise kuyo iDNA, ezi ndlela zibonelela ngovakalelo oluphezulu kunye nobuchule, ezibalulekileyo, ezibalulekileyo ekufumaneni ii-CNvs zemfuza.
● Ngabaninzi izibonelelo ze-PRCRX PR kwi-genection
I-Accrx PCR iphucula ukusebenza kakuhle kovavanyo lwemfuza ngokuvumela ukwahlula-hlula kweethagethi ezininzi ngaphakathi kwendlela enye. Oku akugciniwe kuphela kolondolozo lwesampulu exabisekileyo kuphela kodwa kunciphisa ixesha kunye neendleko ezinxulumene nohlalutyo lwemfuza, zisenza ukhetho olukhethiweyo phakathi kwabavelisi beekhibhodi ze-gene.
Izicelo kuPhando lwe-bioMedical
● Sebenzisa umhlaza kunye nomhlaza wemfuza
I-Gene Copiation Audetions Darives Dlala indima ebaluleke kakhulu kwi-pathogenesis ye-phargenesis yomhlaza ohlukeneyo kunye nemfuza. I-KITS njengeikhilopiki zeseli ye-Tene Gene Neeki yeXesha leMfundo zezokuchonga ezi zahluko, ezivunyiweyo zisondela kwiindlela zokuphucula izigulana.
● Iimpembelelo zemveliso ye-vecral vector
Kwimveliso ye-vicral vector, ukuqinisekisa ukuba inombolo yekopi echanekileyo ibalulekile ekusebenzeni kakuhle nasekukhuseleni. Ukusetyenziswa kwe-gene cop cop Ands Iikhithi zeNkqubo iqinisekisa ukuba iivenkile ziveliswa kunye nomvuzo onqwenelekayo wemfuzo, ekhuthaza amandla abo okunyanga.
Ukuchaneka kunye nokusebenza kakuhle kwiprojeknoloji yeNgingqi
● Ukubaluleka kokubaluleka kokuchaneka kwesiko
Ukuchaneka kwi-gene yokukhuphela kubalulekile kuzo zombini phando kunye nezicelo zeklinikhi. Iimpazamo kwi-dosage yemfuza inokukhokelela ekuzisweni okungachanekanga okanye unyango olungafanelekanga. Ukuchaneka kwanikezelwa kwezixhobo ze-depperive ye-depperive ye-pheep, ezinjengeblikit, iqinisekisa ukuba uhlalutyo lwemfuzo luzaliseke kwaye liphinde libekwe.
● Le khit iqinisekisa njani iziphumo eziphezulu zokuchaneka
Ngoyilo olucekeceke kunye novavanyo olungqongqo, i-Gene ye-Gene ye-Gene ye-Vele yeKithi zeKits zizisa iziphumo eziphezulu zokuchaneka. Ikhithi nganye ihlawulwe ukunciphisa ukwahluka kunye nokwandisa ukuchaneka, ukuseta umgangatho wezinto ze-gene yeKitoko ikopi ye-Gene Shicilelo kwihlabathi liphela.
Umsebenzisi - uyilo olunobuhlobo kunye neeprotocol ezilungisiweyo
● Ukwenza lula iinkqubo zovavanyo lwemfuza
Ubunzima bovavanyo lwemfuza buhlala bubeka imiceli mngeni kubaphandi. I-Bluekit ijongene nokuneemveliso ezingasiki nje kuphela - Umphetho kodwa uyafumaneka kwakhona. Iiprotokholi zabo ezilungelelanisiweyo zenza lula inkqubo yovavanyo, ivumela abaphandi ukuba bagxile ekuthinteleni iziphumo kunokuba kuvavanye iingxaki.
● Ixesha - Ukugcina imiba yemveliso yeBluekit
IXESHA LOKUSETYENZISWA KOMSEBENZI OKANYE I-BLEKIT YOKUGQIBELA I-Veee Ceed Cop. Ngokunciphisa inani lamanyathelo kwaye kubandakanya okulungile - Sebenzisa ii-Regegents, ezi kits zigcine ixesha elixabisekileyo kunye nezixhobo, zibenza babe yinto entle phakathi kwabathengisi beekhilotha zeTene.
Isisombululo esibanzi kunye nejika eliqhelekileyo
● Izibonelelo zokuba ne-- kwi-curve esemgangathweni
Yakhiwe - kwi-curve esemgangathweni yinto ephambili ebeka iimveliso zeBlueKit ngokwahlukeneyo. Iqinisekisa ubungakanani bokuguqulwa kovavanyo, ukunciphisa amandla emposiso yomntu kunye nokuphucula ukuthembeka kweziphumo.
● Ukuncitshiswa kwempazamo yomntu malunga ne-Pre - Seta
Phambi - Seta iiparameters Olu kuncitshiswa kwempazamo yomntu kubalulekile ekugcineni ukuthembeka kohlalutyo lwemfuzo.
Izifundo Zezifundo kunye neYona - Izicelo zeHlabathi
● Imizekelo yophando oluyimpumelelo usebenzisa ikhiti
Izifundo zophando ezininzi zisebenzise iimveliso ze-Bluekit ukuqhubela phambili ukuqonda kwabo iinombolo zezokhuseleko ze-gene. Umzekelo, izifundo zomhlaza zakutshanje zisebenze ezi kiti zokuchonga ii-CNVs eziBalulekileyo ezinxulumene nokuqhubela phambili kwe-tumor kunye nokunyangwa konyango.
● Ubungqina obuvela kubaphandi bemfuza
Abaphandi abakhokelayo ebaleni baye bakhuphela i-gene cop is kwi-bluekit ngokuchaneka kwabo, ukulungiselela ukusetyenziswa kwabo, kunye nokuthembeka. Ezo zigqibo zigxininisa isikhundla sekhithi njengokhetho oluphambili kwi-ENEECO EKHONI
Ulwakhiwo lwexesha elizayo kuhlalutyo lwenombolo ye-gene
● Ukunyuka okunokubakho kuvavanyo lwemfuza
Ikamva lovavanyo lwemfuza Ubuxoki ekuhlanganiseni kwetekhnoloji ye-teinties enobuchule kunye neendlela zokusebenza. Njengoko intsimi ivela, i-Bluekit ilungele ukukhokela intlawulo, ukuphuhlisa izisombululo ze-Necuphument ezityhutyha imida yento enokwenzeka kwi-gene captions.
● Izisombululo ze-Bluekit zinokubumba njani uphando lwexesha elizayo
Ukuzibophelela kweBluekit ekuqinisekiseni izinto ezintsha kuqinisekisa ukuba ihlala iphambili kuphando lwemfuza. Ngokuqhubeka ngokucofa iikhilogre zekopi yeKits, babonelela abaphandi ngezixhobo ezifunekayo ukuvula izinto ezintsha zokufunyanwa kwesayensi kunye nezonyango.
Malunga ne-bluekit
I-jiangsu yaseJiangsu, esebenza phantsi kweBluekit yeBluekit, ihlawulwe eSuzshou enezityalo ze-GMP ezinzulu kunye ne-R & D. Baqhayisa ngeendawo zokuvelisa i-Shenzhen naseShanghai, kunye nobukho obuzayo kwi-North Carolina, eUnited States. I-BlueKit isebenza kwiiqonga eziphuhlayo kwiimveliso zonyango lweselula, i-T, TCR - Ukuzinikela kwabo kulawulo lomgangatho kunye nokuphuculwa kwemveliso yeselula kwizikhundla ezikwiinkokeli ebaleni, zinikezelwe ekuqhubeleni iindlela zeselula kwizibonelelo zoguli zehlabathi.
IXESHA LOKUQALA: 2025 - I-02 - 28: 11:58:08
