Intshayelelo
Intsimi yonyango lweseli ibone inkqubela ebalaseleyo kule minyaka ilishumi idlulileyo, ngakumbi ekunikezelweni nasekusebenziseni iiseli zendalo (NK). La masele akhuselekileyo, adlala indima ebalulekileyo kwiindlela zokuzikhusela umzimba, athabathe i-pivotal ekuphuhliseni i-nooveli yolawulo lomhlaza kunye nezinye izifo. Eli nqaku lidla ngokufika kwintsundu ye-NK ye-trophobiast kunye ne-trophoblast ye-trophoblast kunye neeseli ze-K562 ze-k562, ukubaluleka kwe-cytokines, kunye nezikhombisi zexesha elizayo ze-NK. Ukongeza, sichukumisa ukubaluleka kweI-DNA ye-DNA ye-DNA Preproceceng Kits ekuqinisekiseni umgangatho kunye nokhuseleko lweemveliso zonyango lweseli.
Amagqabantshintshi nge-NK ye-NK
● Ukubaluleka Kokunyanga kweseli
I-NK yeeseli zokwandiswa kwezixhobo ezibaluleke kakhulu kwicandelo lonyango lweseli, ivumela ukwenziwa kweeseli ze-NK kumanqanaba onyango. Ezi kits ziququzelela ukuveliswa kwenani elaneleyo leeseli ze-NK ezifunwayo kwizicelo zeklinikhi, ngaloo ndlela kuphucula amandla abo.
● Izinto zekhithi
Ngokwesiqhelo, i-NK ye-NK yeekiti zokwandiswa kwezinto ezibandakanya iiseli ze-feeder, i-cytokenes, kunye ne-basium ye-NK ye-NK eseleyo. Ezi macandelo zisebenza ngokudibeneyo ukuba zenze imeko efanelekileyo yokuphezulu kwe-NK kunye nokuSebenza, ukuqinisekisa isivuno esiphezulu nobunyulu.
Indima yeeseli zeTrophoblast kwi-NK isebenze
● Indlela yokukhuthaza
Iiseli ze-trophoblast zisebenzisane ngokwendalo kunye neeseli ze-NK ngexesha lokukhulelwa, ukudlala indima ebalulekileyo ekusombululeni umsebenzi wabo. Kwimeko yokwandiswa kwe-NK
● Izibonelelo zokusebenzisa iiseli zeTrophoblast
Sebenzisa iiseli ze-rophoblast kwi-NK yokwandiswa kubonelela ngendlela eyahlukileyo ye-NK yokuvuselela iiseli ze-NK, ezinokubakho ezinokubakho kwiiseli ezisebenza kakhulu xa kuthelekiswa nendlela eyandisiweyo.
Iiseli ze-K562 ze-K562 kwi-NK yolwandiso
● I-Cytokiine intetho kwi-K562 yeeseli
Iiseli ze-K562 ze-K562 zisetyenziswa rhoqo njengeeseli zoondla kwi-NK yokwandiswa kwe-NK. Batshintshiwe ukuze baveze i-cytokines enje nge-IL - 15 kunye ne-Il - 4, ezibaluleke kakhulu kukukhula kwe-NK kunye nokuSebenza.
● Impembelelo ye-Iradiation kunye ne-Ikhutshiwe
I-Iradiation okanye i-Ikhutshwe kwiiseli ze-K562 ngaphambi kokusetyenziswa kwabo njengoko iiseli zondliweyo ziqinisekisa ukhuseleko olungafunekiyo, ngelixa ugcina amandla abo okuxhasa ukwanda kwe-NK.
Ukubaluleka kweCytokines kwi-NK
● Il - 21 Neziphumo zayo
I-cytokenes ifana nel - I-21 zibalulekile kwi-NK yesel. I-Il - 21 Ayikhuthazi kuphela i-NK ye-NK kuphela kodwa ikhuthaza imisebenzi yawo ye-cytotoxi, esenza icandelo elibalulekileyo le-NK yeekhilomitha zokwanda kwe-NK.
● Indlela yokulinganisa ukulinganisa
I-Cytokines isebenza ngezami ezahlukeneyo zokubonisa ukuvula iiseli ze-NK. Ukuqonda ezi ndlela kubaluleke kakhulu ekupheliseni iiprothokholi zokwanda kwe-NK zeseli kunye nokuphucula ukusebenza kwazo ekusebenziseni.
Imithombo ye-NK ye-NK: I-umbilical cord factor yegazi
● Ukuthelekisa isivuno seseli
Igazi le-Ibilical Igazi ngumthombo otyebileyo weeseli ze-NK, uhlala evelisa amanani aphezulu xa ethelekiswa negazi labaphene. Nangona kunjalo, ukhetho phakathi kwemithombo luxhomekeke kwiimfuno ezithile zesicelo sonyango.
● Ukucoceka kunye nomgangatho weeseli ezandisiweyo zeNK
Zombini imithombo zinokubonelela ngeeseli eziphezulu ze-NK, kodwa iiprothokholi zokwanda zinokwahluka. Ukuqinisekisa ubunyulu kunye nokusebenza kweeseli ezikhoyo ze-NK zibaluleke kakhulu kwiziphumo zeklinikhi eziphumeleleyo.
Isicelo semoto - I-NK yeseli
● Iinkqubo zokufumana imoto - Iiseli zeNK
Imoto - Iiseli ze-NK zinikezelwe ekuvezeni izinto ze-chimeric antign, zokwenza ukuba zijolise kwiiseli ezithile zomhlaza. Ukulungiswa kwemoto - Iiseli ze-NK zibandakanya ukuguqulwa kwemfuza kunye nokwandiswa, iinkqubo eziqhutywa ziikhithi zemfundo ezizodwa.
● Izibonelelo ngeendlela ngeendlela zemveli
Imoto - Iiseli ze-NK zibonelela ngeenzuzo ezininzi kwiindlela zemveli, kubandakanya umngcipheko weGraft, ukuthelekiswa - Isifo se-Insances kunye nesakhono sokujolisa kuluhlu olubanzi lwee-atronis zomhlaza.
Inkqubo yophuhliso lonyango lweseli
● Ixesha kunye nokusebenza kwexabiso kuphuhliso lwakwangoko
Ukuphuhliswa kweenkqubo ezifanelekileyo zonyango lwe-NK kubalulekile ukunciphisa ixesha kunye neendleko kwizigaba zophuhliso kwangoko. Iiprothokholi ezisebenzayo kunye nokufumana i-curive - kwi - Sebenzisa izixhobo zokwandiswa kunokwakhethela le nkqubo.
● Izicwangciso zokuphelisa iimveliso zonyango
Izicwangciso ezifana nokuzenzekelayo, ukuqhubekeka kwenkqubo evaliweyo, kunye nokusetyenziswa kwe-microfial - Iirejista zasimahla zibalulekile ekufezekiseni umgangatho kunye nokungaguquguquki kweemveliso zonyango lweseli.
Ukuphucula ubunyulu kunye nesivuno seeseli ze-NK
● Iindlela zokufezekisa ubunyulu obuphezulu
Iindlela zobuchule ezinje nge-cytometry ye-cytometry - Ukuhlelwa kobungakanani kunye nokwahlulahlula imagneti kuqeshiwe ukufezekisa amanqanaba aphezulu okucoceka kwiiseli ezandisiweyo ze-NK, ezibaluleke kakhulu kwizicelo zeklinikhi.
● Imiceli mngeni kunye nezisombululo ezikhulu - Ukwanda kwesikali
Ukuqhekeka kolwandiso lwe-NK kubibangela imiceli mngeni enjengokugcina ukusebenza kweseli kunye nokusebenza. Uyilo lwe-bioreactor lol entsha kunye neenkqubo eziqhubekayo zokubeka iliso zizisombululo kule miceli mngeni.
Izibonelelo zokusebenzisa i-NK Sell Assium
● Indima yokwandiswa kweseli kunye nokuSebenza
I-NK Sell I-Wised Medium iqulunqwe ngokungqalileyo ukuxhasa ukukhula kunye nokusebenza kweeseli ze-NK, ekuqinisekiseni ukusebenza okungaguqukiyo kwiipropuokhosi ezahlukeneyo.
● Ukuhambelana neeCytokines ezahlukeneyo
Ukuhambelana kwephakathi kunye noluhlu lwe-cytokines kuvumela ukuguquguquka xa kunyanzeliswa ukwenziwa kokwandiswa kwe-NK ukufezekisa iimfuno ezithile zonyango.
Izikhombisi zexesha elizayo ze-NK yonyango lwe-NK
● Iindlela ezivelayo kunyango lweseli
Intsimi ye-NK yonyango lwe-NK ivela ngokukhawuleza, kunye neendlela ezivelayo zigxile ekuphuculeni ubuchwephesha, ukunciphisa iziphumo, kunye neziphumo zendawo ekujoliswe kuyo, kunye nokuphucula ukukhululeka konyango.
● Ukunyuka okunokubakho kwizicelo ze-NK
Inkqubela phambili yexesha elizayo inokubandakanya ukuphuculwa kweeseli zoncedo lwe-NK, ukudibanisa unyango kunye nolunye uhlobo lwamakhaya, kunye neeseli ze-NK ze-NK zilungiselelwe iiprofayili zomntu ngamnye.
Ukuqinisekisa ukuba kumgangatho we-DEAMP ye-DNA i-DNAPPOPOCOPOPORK
● Ukubaluleka Kokunyanga kweseli
I-DNA ye-DNA i-DNA ibalulekile kunyango lweseli ukuze ifumane kwaye ithathe i-DNA ye-DA yendawo yokuhlala. Ezi kiti ziqinisekisa ukhuseleko kunye nokusebenza kakuhle kweemveliso zonyango lweseli ngokunciphisa umngcipheko wongcoliseko.
● Indima yabavelisi kunye nabathengisi
Abavelisi kunye nabathengisi be-DNA ye-DNA i-DNAPPOP kits, njengeI-Bluekit, dlala indima ebalulekileyo ekunikezeleni ukuthembeka kunye ne-bits esemgangathweni. Ezi kokii zibalulekile ekugcineni ukuthembeka kweenkqubo zemveliso yeseli.
Ukuqukumbela
Imbonakalo yonyango lweseli ihlala ivela rhoqo, kunye neeseli ze-NK kwangaphambili kwale profazwe. Ukudityaniswa kwe-neight yokwanda kokwandisa, i-Cytookine Fights, kunye nezixhobo zoQinisekiso lweMigangatho elifana ne-DNA kwi-DNA yokuqhuba i-DNA iqhuba uphuhliso lonyango olukhuselekileyo nolukhuselekileyo. Njengoko ishishini liqhubela phambili, intsebenziswano phakathi kwabaphandi, abagqirha, kunye nabavelisi baya kuba sisitshixo sokuvula amandla apheleleyo e-NK.
Jiangsu Hillgene, phantsi kweBlueKit ® imele ngaphambili kwe-serapy yonyango lweselula. Ikomkhulu layo eSuzshou kunye nemveliso eyenza imveliso kwi-China naseSorth Carolina, induli ingamaqonga ovulabuvulayo yemveliso ye-nucleic kunye nemveliso yemveliso yonyango lwemveliso. Ukuzibophelela Kulawulo loMgangatho, iimveliso zeBluekit ® kuqinisekisa ukusebenza ngonyango, ukuxhasa amaqabane ehlabathi ekufezekiseni imoto ephumeleleyo - I-T, i-T, i-STATE Iseli - Ngombono wokukhuthaza ubuchule beseli ekhuthazayo, induli inikezelwe ekuqhubeni izisombululo zonyango zeselula kwihlabathi liphela.
IXESHA LOKUQALA: 2024 - 12 - 13 15: 31:09
