1. Intshayelelo kwi-BlueKit's Il - 15 i-15 le-LISAT
● IBALULEKILEYO KWI-CYTOKINE Uhlalutyo
I-cytokines, iiproteni zemolekyuli eziphantsi, zidlala indima ebaluleke kakhulu ekwisiphatho. Babalulekile ekucingesisweni kwenkqubo yomzimba, ukuphembelela ukukhula kweseli, kunye nokucamngca ukuvuvukala. Ukufunyanwa ngokuchanekileyo kunye nokugqitywa kweCytokines kunyanzelekile ukuba kufezekiswe ukuqonda kwethu iinkqubo eziqhelekileyo zempilo kunye neemeko ze-pathofor. Phakathi kwale Cytokines, i-Enterleukin 15 (il - 15) imele ngenxa yeempembelelo zayo ezinzulu kumgaqo-nkqubo wempendulo yomzimba. I-Bluekit'sIl - 15 I-15 le-LISSAinikezela ngesixhobo esinokuthenjwa kubaphandi esijolise ekuphelisweni kwenzululwazi ye-cytokine, ukubonelela ngokuchanekileyo kunye nokuchaneka kwe-IL - Amanqanaba ali-15 kwiisampulu ezahlukeneyo zebhayoloji.
● Indima yoPhando lweMveliso
I-Inform yeNkqubo yokuzikhusela yinethiwekhi entsonkothileyo yeeseli kunye neemolekyuli ezisebenza ngokuhambelana nokukhusela umzimba kubahlaseli bangaphandle kwaye ugcine ulinganiso lwangaphakathi. Il - 15 yi-cytokine egxekayo kule nothunjelwano, eyaziwa ngokubanakho ukonyusa ukwenziwa kunye nomsebenzi we-Biller (NK) kunye ne-T LYMPHISCSTSE. Ukuqonda il - Umsebenzi we-15's kunye nefuthe layo lokuphendula amajoni kubalulekile ekuphuhliseni unyango lwezifo ze-autoimmune, kunye nezifo ezosulelayo. I-BlueKit il - 15 I-15 i-15 i-15 ye-ELISA yenzelwe ukunceda abaphandi ekuthintelini iindlela ezinzima, zibonelela ngeqonga le-Il - 15 kwibhayoloji ye-IL.
2. Ukuqonda il - 15 kwi-15 kwi-15 kwi-15 kwi-15 kwi-15 kwi-15
● Impembelelo kwiiseli ze-T kunye neeseli ze-NK
Il - 15 Udlala indima ebalulekileyo kuphuhliso, ukusinda, kunye nokwanda kweeseli ze-T kunye neeseli ze-NK. Obu batoli bezikhuni zezinto eziphambili zendlela yokuzikhusela komzimba ngokuchasene namathumba kunye neeseli ezosulelekileyo. I-Il - Le propati iyenza il - 15 Umgqatswa othembisayo wongenelelo ngonyango ngomhlaza umgubo kunye nogonyo, apho i-cytotovis ye-cytotovity iyingenelo.
● Impembelelo kwi-Dynamics yokuphendula
Ngaphandle kwendima yayo e-Lymphocyte, IL - 15 Inegalelo kumgaqo wokuvuvukala kunye nogonyamelo. I-IT morlates ibhalansi phakathi kwe-pro - ukuvuvukala kunye ne-Anti - Iimpendulo ezivunyiweyo, ukwenzeka, ukonakalisa ikhosi kunye nesisombululo samago amsa. Uphando usebenzisa il - 15 i-ELISAKIT, njengaleyo ivela kwi-Bluekiit, ibonelela ngokuqonda ukubandakanyeka kweCytokine ekusebenzeni kweendlela ezinobunzima bokwenyama, ukuthobela ukuphazamiseka kwe-autoimmune.
3. Ukubaluleka kwe-IL echanekileyo - Ukufunyanwa kwe-15
● Ukubaleka kwi-Immunology kunye nokuvuvukala
Ngokuchanekileyo ukunciphisa i-IL - Amanqanaba ali-15 kubalulekile ekuqhubeni uphando kwi-itronology kunye nosulelo. I-IL - I-15 intetho inxulunyaniswa neemeko ezahlukeneyo ze-parethological, kubandakanya izifo ezingapheliyo zokukhuphela, i-autoimmunity, kunye nomhlaza. Ke ngoko, i-il echanekileyo - 15 I-15 Kit iKhithi ye-ELISA iyimfuneko ekuchongeni ezi meko, ukubeka esweni ukuqhubela phambili izifo, kunye nokuvavanya ukusebenza kakuhle konyango.
● Izicelo zophando lwebhayoloji yomhlaza
I-Il - 15 iye yavela njengearhente enokubakho kunyango lomhlaza ngenxa yokwandisa umsebenzi we-cytotoxic ye-Nk kunye neeseli ze-T. Ngokulinganisa ngokuchanekileyo il - Amanqanaba ali-15 asebenzisa il ye-Bluekit Il - Ngapha koko, il -
4. Izibonelelo ze-ELISA ye-ELISA
● Ukuchaneka kunye neemvakalelo
I-Il ye-Bluekit - 15 I-15 i-15 ye-ELISA iyile ichanekile kunye nemvakalelo, ukuqinisekisa inani elithembekileyo le-IL - 15 nakwiisampulu zoxinaniso eziphantsi. Itekhnoloji yayo yokuNgcocwa kweXhengiso leThengiso iLibala
● Umsebenzisi - Uloyilo olunobuhlobo kwaye oluthembekileyo
I-ELISA Kit evela kwi-Bluekit ilungelelaniswe ngokulula, iyenza ilungele abaphandi kumanqanaba ohlukeneyo obuchule. Iprotocol ethe tye kunye nesikhokelo esibanzi sedatha esisiqhamo siqhuba umdibaniso ongenangqondo kwi-labhoratring yokubhukuda, ukuqinisekisa ukusebenza ngokuthembekileyo kunye nokuxhomekeka kwedatha yovavanyo olwahlukileyo.
I-5. Ulwazi: I-Pauter
● Ukucaciswa kweqonga le-ELISA
I-Paunt - I-Antictur Sundwich Elica Geneiquique Il Il - Kubandakanya ukusetyenziswa kwezinto ezimbini ze-antibodies: i-antid ankntsu efakwe kwi-microplate kunye nokufunyanwa kwe-antiddmed kwi-enzyme. Le ndlela iqinisekisa ukubopha kwe-IL: 15 kwisampulu, kulandelwa yindlela yokusabela kwimodirimitha ngokulinganayo kwi-cytokine gxini, yenza ukuba kufunyenwe ibuthathaka.
● Ngokuqhelekileyo unxibelelwano oluchasene nolwanti
Ubuchule bokuphindaphindiweyo - Ubuchule be-anticled bunder bume kwi-antibodies ekhethekileyo ebophelelekileyo kwi-epitoes eyahlukileyo ye-IL - 15. Olu bukhulu bobu buthathaka lunciphisa ingxolo yemvelaphi kwaye luphucula imvakalelo ye-Assay, eyenza i-il ye-Bluekit's - 15 i-ELISA KIT isixhobo esinamandla se-cytokine echanekileyo kwizicelo ezahlukeneyo zophando.
6. Isampulu yesampulu
● Ukufunyanwa kwiSerum, iPlasma, kunye ne-Superntuents
I-BlueKit's Il - Oku kuqabela phambili kwenza ukuba kubekho isixhobo esifanelekileyo kubaphengululi be-Il - I-15 i-15 ekwakheni kwii-Matric ezahlukeneyo, ngokuququzelela uhlalutyo olubanzi kwiiyilo ezahlukeneyo zokufunda.
● Ukulinganisa iziphumo ezingaguqukiyo
Ukuqinisekisa ukungqinelana kunye nokuchaneka, i-IL - Ukufakwa kwemigangatho elungelelanisiweyo kunye nolawulo luqinisekisa ukuba iziphumo ezivunyiweyo zibuyise iziphumo, zivumela abaphandi ukuthelekisa idatha kuzo zonke izilingo kunye nokufikelela kwizigqibo ezifanelekileyo.
7. Isikhokelo somsebenzisi kunye nokutolikwa kwedatha
● Ushwankathelo lwedatha yedatha
Idatha ebanzi enikezwe kunye ne-Bluekit il - I-15 le-15 i-15 ibonelela ngemiyalelo eneenkcukacha malunga nokuseta kwe-ASS, isampulu, kwaye icacise. Esi sibonelelo sisebenza njengesikhokelo esixabisekileyo, ngakumbi kubaphandi abatsha kwiTekhnoloji ye-Elisa, ukuqinisekisa inkqubo yovavanyo oluyinyani kunye nohlalutyo lwedatha oluchanekileyo.
● Inkqubo elula ye-ELISA ATSHA
Ukuqonda ubunzulu be-ELISA Assays kunokubangela ukuba abaqalise. I-Bluekit ijonge le nkqubo ngokwenza lula inkqubo, inika inyathelo elicacileyo - Imiyalelo yeMiyalelo kunye neMiyalelo yokuBeka iNkqubo yokuzithemba kwaye ifumane iziphumo eziqinisekileyo.
8. Ukuphucula uphando nge-EIFS ye-ELIITS
● Ukuqhutywa kwezinto ezifumanekayo kwi-Immunology
Ukusetyenziswa kwe-Il yeBluekit's - 15 i-ELISA ikhithi ixhobise abaphandi ukukhangela intshukumo yendlela yokuthuka amajoni omzimba kunye neenethiwekhi zeCytokine. Ngokubonelela ngedatha yexabiso elichanekileyo, iququzelela ii-Inoves ezifumanekayo kwi-Noves kwi-Immunology, ityhila indlela yokuqonda okutsha ekwenzeni amajoni omzimba kunye neethagethi ezinokubakho.
● Ukuxhobisa abaphandi ngezixhobo ezinokuthenjwa
I-Bluekit izibophelele ekuboneleleni ngezixhobo eziphakamileyo zophando ezihlangabezana neemfuno ezingqongqo zophando lwezenzululwazi. Nge-il yayo - I-15 le Kit, iBebkit ixhasa abaphandi ngokunikezela ngeqonga eliqinisekileyo lokuzivavanya, ngaloo ndlela ukwahlula-hlulwa kweyona bhayoloji yeselula nemolekyuli.
9. I-Innoveration Inveration kwizixhobo ze-biology yezixhobo zeselula
● Igalelo kwiNovel Cunter
Kwindawo yokugcina izinto ze-biotechnology, ukuphuculwa kokusika - Izixhobo izixhobo zibalulekile ekuqhubeni i-Innovation yonyango. I-BlueKit's Il -
● Ukuqhubela phambili ukuqonda kweenkqubo zeselula
Uhlalutyo oluneenkcukacha lwenziwe i-Il ye-Bluekit - I-15 i-15 i-15 i-15 i-15 i-15 i-15 i-15 i-15 i-15 i-15 i-Elsa ivumela abaphandi ukuba banciphise iinkqubo zeselula kunye nokusebenzisana kwamajoni. Oku kuqonda kubalulekile ekuphuhliseni iindlela zokungenelela ekujoliswe kuzo ezichaza iimpendulo zomzimba, ekugqibeleni zikhokelela kwiziphumo eziphuculiweyo kunye nokuguqula iparadigms.
I-10. Ukuzibophelela kumgangatho osemgangathweni kunye nokuhambela phambili kwesayensi
● Ukuzinikezela kweBluekit kwiPrinta Teight
I-Bluekit iqinile ekuzibopheleleni kwayo ekugqweseni kuphando lwenzululwazi, ukuhambisa izisombululo ezinobuchule ezihlangabezana nemiceli mngeni yophando lwebhayili. Nge-il yayo - 15 I-Elati Kit, iBluekit ihambisa umgangatho osemgangathweni kunye nokusebenza, ukuqinisekisa ukuba abaphandi banezixhobo abadingayo ukufikelela kuzo.
● Amandla exesha elizayo kwi-biomeds
Ukujonga phambili, i-Bluekit inombono wexesha elizayo apho iimveliso zayo ziyaqhubeka nokuqhuba inkqubela phambili kwi-biopositical entsha. Ngokuxhasa ukuphuculwa konyango lwethamo kunye nokuphucula ukuqonda kwethu kweenkqubo zebhayoloji ezintsonkothileyo, iBluekit ijolise ekufakweni kwegalelo elihleliyo kwimpilo yehlabathi.
Malunga ne-bluekit
I-jiangsu yaseJiangsu, phantsi kwegama lophawu lwe-Bluekit, ihlawulwe eSuzshou, eneemveliso zokwenza imveliso eyahluliweyo kwilizwe lonke kunye nobukho behlabathi Carolina, eUSA. Ukuziphakamisa kuphuhliso lweemveliso zonyango zeselula, i-Bluekit ibonelela ngamaqonga e-Innovative yemveliso ye-nucleic ye-nucleic scocid kunye ne-serum - Isicamango simahla. Ukuzibophelela kwinkqubela phambili yonyango lweselula, i-Bluekit ixhasa uphuhliso lwemoto - T, i-T, TCR -
IXESHA LOKUQALA: 2024 - 12 - 17 15:41:05
