Cell cytotoxicity assan kit (lisele tse atlehileng)
Cell cytotoxicity assan kit (lisele tse atlehileng)
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✔ Cfse - Lisele tse peli tsa Target bakeng sa tlhaiso e hlakileng
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✔ 7 - AAD e thetsa bakeng sa senoelo se nang le seli se setle se nepahetseng
✔E etselitsoe Mehala ea Cell ea TumOr Cell
Kakaretso:
Kit e etselitsoe ho lekanya Cesector Cell cytotoxicity khahlano le lisele tse bohloko, e loketseng Lisele tsa NK, lisele tsa NK, Car - Lisele tsa Tr - Lisele tsa Tr - Lisele tsa TM Tumor - Mapolanka Assays.
Likarolo tsa Kit:
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Cfse .
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7 - AAD (7 - Aminoactinomycin d, DNA - dee e tlamang bakeng sa senoelo se shoang)
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Medium B (E ntlafalitsoe bakeng sa cytotoxicity assays)
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Assay Buffer (E netefatsa maemo a thata a anane)
Likopo tsa Bohlokoa
✔Tlhahlobo e sebetsang ea lihlahisoa tsa sele ea 'mele (e.g., koloi - lisele tsa NK) Khahlano le Mehala ea Cell ea TumOr
✔Tsamaisa nts'etsopele le Optimization Patlisiso ea The ea Cell Therapy
✔Ho lokolloa liteko le lithuto tsa botsitso Bakeng sa GMP - Ho fana ka lihlahisoa
Lipontšo tsa Ts'ebetso:
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Ho ngolisoa ka mokhoa o kopaneng (Cfse bakeng sa liphofu, 7 - AD bakeng sa Cytotoxicity)
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Protocol e tloaelehileng Bakeng sa sephetho sa tlhahiso
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E tsamaisana le PUP - Litsamaiso tsa ho kenya (96/384 - Mamati a Word)
Tsamaiso ea Tsamaiso le indasteri
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E netefalitsoe bakeng sa Mehala ea sele ea Tumor (e.g., hela, A549, MCC - 7)
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E loketseng Thailand - Lithuto tsa Ho Eena (Ich Q2 (R1) e lumellanang)
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21 CFR Karolo 11- Ho tlaleha data e lokiselitsoeng
Nk - polao e ikhethang ea Cfse + lisele tsa A549 tse nang le phallo ea lipalesa
NK - polao e ikhethang ea polao ea CFSE + e tiileng ea tumoor e tiileng ka ho phalla Cytometry
Leqephe la lintlha:
| Mycoplasma Tin | E ntle / kakaretso | Li-rantiste tse amanang haufi-ufi | E ntle / kakaretso | Lactogacillaus acidophilus | Streptococcus pneumoniae | Cloesrium Acetobutylicum |
|---|---|---|---|---|---|---|
| Mycoplasma Orale | 24/24 | Mycoplasma Rathridididis | 24/24 | Mpe | Mpe | Mpe |
| Mycoplasma Gallisecticum | 23/24 | Mycoplasma arginini | 24/24 | Mpe | Mpe | Mpe |
| Atholeplasma Laidlawii | 24/24 | Mycoplasma Hushininis | 24/24 | Mpe | Mpe | Mpe |
| Barcoplasma barmagens | 23/24 | Spiroplasma citri | 24/24 | Mpe | Mpe | Mpe |
| Mycoplaya Pneumoniae | 24/24 | Samcoplasma salivarium | 24/24 | Mpe | Mpe | Mpe |
| Catalog No. | Karolo | TLHOKOMELISO | Mocheso oa polokelo | Boemo ba kit |
| HG - Md002 | Medium B | 100 ml | 2 - 8 ° C | Kit i |
| HG - Fw001 | Assay Buffer | 100 ml | 2 - 8 ° C | - |
| Hg - cfse | Cfse | 1 vial | - 20 ° C | Kit II |
| HG - 7 - AAD | 7 - AAD | 1 vial | - 20 ° C | - |
TLHOKOMELISO
| Paramente ea Ts'ebetso | Ho amohela litekanyetso |
| Ho nepahala | 70-130% Pholoso |
| Ho Tlaleha | 70-130% khethoa |
| Ho Phethahala | CV <10% |
| Khopolo-taba ea Bohlale | CV ≤ 10% |
Tlhahisoleseling ea ho tsamaisa
Re fana ka lipalangoang tse sephara ho litaelo tsohle. Ka tloaelo, odara ea hau e tla fihla leqepheng la 5 - matsatsi a khoebo a 7 United States le matsatsing a 10 a khoebo bakeng sa linaha tse ling. Leha ho le joalo, ka kopo hlokomela hore ho isoa ho libakeng tsa mahaeng ho ka nka nako e telele.
Nako ea ho tsamaisa: Litaelo li sebetsoa nakong ea 1 - matsatsi a 3 a khoebo. Hang ha odara ea hau e se e rometsoe, u tla fumana lengolo-tsoibila le netefatsang ka tlhaiso-leseling.
Tlhahisoleseling ea Bohlokoa
Ho sebetsa ha taolo: Kamora hore taelo e se e lefiloe, ntlo ea rona ea bophelo e hloka nako ea ho sebetsa odara ea hau. U tla fumana tsebiso hang ha odara ea hau e rometsoe.
Mefuta ea ho tsamaisa: Maemong a mangata, sephutheloana se tla tsamaisoa ka nako e lekantsoeng ea ho fihla. Leha ho le joalo, letsatsi la pele la tlhahiso le ka angoa ke litokisetso tsa lifofane, maemo a leholimo le lintho tse ling tsa kantle. Nako ea ho tsamaisa thepa e tla be e le telele ho feta e tloaelehileng bakeng sa litaelo tse kenyelletsang mokhoa oa pele kapa lintho tse hlophisitsoeng. Ka kopo sheba tlhaiso-leseling ea ho batla bakeng sa letsatsi le nepahetseng ka ho fetisisa.
Litaba tsa ho tsamaisa: Haeba u fumana hore sephutheloana sa hau ha se so lopolloe ka nako e boletsoeng; Tlhahisoleseling ea ho lelekisa e bontša hore sephutheloana se pholositsoe empa ha u e amohetse; Kapa sephutheloana sa hau se kenyelletsa lintho tse sieo kapa tse fosahetseng kapa mathata a mangata, ka kopo ikopanye le ts'ebeletso ea bareki nakong ea matsatsi a 7 e le hore re ka sebetsana le litaba tsena hang-hang.
Ho latela tumello
Hang ha odara ea hau e romelloa, u tla fumana lengolo-tsoibila le nang le nomoro ea ho batla le sehokelo sa ho latela thomello ea hau.
U ka latela odara ea hau ka kotloloho webosaeteng ea rona ka ho kena akhaonteng ea hau mme u shebe nalane ea hau ea odara.
Lithibelo tsa ho tsamaisa
Ka kopo tlatsa aterese ea seterateng ka botlalo, eseng lebokose la Pox kapa aterese ea sesole (APP). Ho seng joalo, re tla tlameha ho sebelisa ems bakeng sa ho tsamaisa (e ea lieha ho feta tse ling, ho nka 1 - ho nka likhoeli tse peli kapa nako e telele.
Mesebetsi ea Litloaelo le Tafole ea Lekhetho
Ka kopo elelloa hore mesebetsi efe kapa efe ea lekhetho, lekhetho kapa litefo tsa ho tlisa thepa tse hlahisitsoeng nakong ea thomello ke boikarabello ba moreki. Litefiso tsena li fapana ho latela naha e eang 'me li khethoa ke ba boholong sebakeng sa lehae.
Ka ho re reka webosaeteng ea rona, o lumela ho lefa mesebetsi efe kapa efe e sebetsang kapa lekhetho le amanang le odara ea hau. Ha re ikarabelle bakeng sa ho lieha ho bakoang ke tumello ea litloaelo.
Phokotso ea "PUSELETSO EA PUSO
Hang ha odara ea hau e fihlile sebakeng se khethiloeng sa Pionep Point kapa sebaka sa ho tsamaisa, ka kopo netefatsa pokello ea potlako. Haeba sephutheloana se sa nkuoe ka nako e behiloeng, re tla romella khopotso ka lengolo-tsoibila kapa sms. Leha ho le joalo, haeba sephutheloana se sa bokelloa nakong e boletsoeng, 'me tahlehelo e teng kapa tšenyo e etsahala ka lebaka leo, moreki o tla jara boikarabelo. Re u hopotse ka mosa hore u bokelle pakete ea hau hang-hang ho qoba litaba life tse ka bang teng.
Tlhokomeliso: Ha sehlahisoa sa rona se oela tlasa sehlopha se khethehileng, ho khutla le theko ha se amoheloe.
