Phapang ke efe lipakeng tsa li-trypsin le liproselaise tse ling?

Kenyelletso ea lipelaelo litsamaiso tsa likokoana-hloko

Lipelaelo, sehlopha se fapaneng sa li-enzyme, ke phetohelo mesebetsing ea likokoana-hloko, ho tloha ho tšila ho tloha ho degmution. Li sebetsa ka mokhoa o hlakileng oa ho hlaka ha peptide ea pepeneneng, ka lebaka leo u bapalang karolo ea bohlokoa ho boloka lehae la lapeng la Cellular. Lipelaelo li thehiloe ho latela mekhoa ea tsona ea catalytic le likarolo tse subang, ka li-sethese tsa Servin joalo ka li-Toppan ho ba tsa tse ithutoang. Sengoliloeng sena se tšohla litšobotsi tse ikhethang tsa trypsin ho fapana le likhohola tse ling, tse sebetsang le likopo.

Trypsin: Tekanyetso ea khauta e maemong a protheome

Karolo ea Trypsin ka Spectrometry

Trypsin e lebisa lebaleng la kokoana-hloko ka lebaka la ho hlaka le ka mokhoa o matla oa ho hloekisa li-carboxy le masala a marang-rang. Ntho ena e felileng e fella ka lits'oants'o tse nang le tefiso e ntle ho C - FERMINUS Li-bustemivis, bokhoni ba ho fana ka bokhoni ba ho etsa boitlamo bo tiileng ba eketsa protheins, karolo ea bohlokoa bakeng sa tlhahlobo ea profe ea limere ea sele ea sele ea sele ea sele ea sele ea sele ea sele ea sele ea sele ea sele.

Melemo ea tlhaiso ea protheine

Li-Bustemics li itšetleha haholo ka thepa e ikhethang ea litente tsa testerin bakeng sa tlhaiso ea protheine. Boithuto bo bontša hore trypsin e ka tsebahatsa protheine e fetang 80% e neng e bapisoa le litlhakoanang le ho e etsa e sa sebetseng bakeng sa bafuputsi le barekisi lebaleng. Karolo ea eona e atisang ho utloisisa ka mekhoa ea bolumeli ho ntšetsa pele mekhoa ea kalafo, joalo ka kalafo ea sele.

Litšobotsi tsa sebopeho sa trypsin

Sebaka sa Mechine e sebetsang le Mochine oa katseno

Ts'ebetso ea Catalytic ea li-trypsin e tsoang ho eona ea li-trypsin e hlaha - Sebaka se hlalositsoeng se sebetsang, se nang le lesela le nang le moetlo oa Sefangine, Hissidine le Aspartate. Khokahano ena e fallisang khokahano ea pele ea bondide ea peptide ea pele, e leng tlhoko ea bohlokoa bakeng sa barekisi ba fanang ka li-enzyme tse hloekisitsoeng bakeng sa lipatlisiso le lits'ebetso tsa indasteri. Ho ikhethang ha enzyme ho buuoa ka boteng ba motho ea qotsitsoeng hampe, e hohelang Сsonine e qositsoeng ka mokhoa o nepahetseng le li-argine.

Istolelo ea trypsin

Trypsin e teng libakeng tse 'maloa tsa Istoforms, ho kopanyelletsa le khatello ea maikutlo le Mesotrypsnogen. Merero ka 'ngoe e sebetsa likarolo tse fapaneng,' me e be le li-candapsin tse ngata haholo ho mapolanka a batho. Mesotryprin e ea tsebahala hore ho hanyetsa ha eona ho hanyetsa li-inhibitors, ho bapala karolo e ikhethang ho senyeha ha liprotheine tsa Inhibitor - liprotheine tse ngata tsa ho ja. Ho utloisisa linaka tsena ho bohlokoa bakeng sa lifektheri tse kenyelletsang tlhahiso ea enzyme bakeng sa tšebeliso ea kalafo.

Liphoso tse ling tsa liaponno le lintho tse ikhethang

Papiso le aspn le gluc

Aspn le Gluc sepheo Acidic acidic, ho fana ka datha ea tlatsetso ho e fanoang ke litente. Li-aprotaranse tsena e hlahisa litlhokahalo tse rarahaneng tsa pepenene tse loketseng litlhoko tse khethehileng tsa tlhahlobo ea liprosetong tsa liprosetong. Leha ho le joalo, ho na le mohopolo oa bona o pharaletseng hangata, khethollo ea bona e hlahisoang ke protheine e fokotsoeng e bapisoa le litente.

Chymotrypsnin le li-costis tse kholo haholo

Chymtostrysin e fapana le trypsin ka ho hlasela masaletseng a maholo a hydrophobic, joalo ka testptophan le tyrorine. Ha e ntse e le katleho e bohlokoa ea catalytic, ho sebelisoa patsi ea eona ha e lekanyetsoe likopo tse itseng. Broad - Litekanyetso tsa Tsitso Joaloka Propeiratoes tse rarahaneng haholo tsa petide haholo, li hlahisa lits'ebeletso tsa liprotheine, ntle le haeba li sebelisoa hammoho le li-Frpsin.

Mekhoa ea litlolo e ngata e nang le trypsin

Boitsebiso bo ntlafalitsoeng ba protheine e ntlafalitsoeng

Ho sebelisoa ha tatellano ea tatellano ea tšebeliso ea li-its'ireletso tse lateloang ke proprote e 'ngoe, li ntlafatsa tsebo ea protheine. Mohlala, profesheleli e pheheletseng ka litente e ka eketsa tlhaiso-leseling ea liprotheine bakeng sa protheines k ka 731%. Bakeng sa likopo tsa kalafo ea phekolo ea 'mele, Sordnergy ena e lumella phaello e phethahetseng ea boithuti, ea bohlokoa bakeng sa mekhoa e utloisisang ea sephara.

Tšireletso ea li-pōpisi tsa litlolo tse ngata

Libibele tse nyane tse hlahisitsoeng ke li-Fispsin li sirelelitsoe ho depestion e 'ngoe ea tatellano ea tatellano, e lebisang ho rarahaneng ha maemo ho feta ho hlahlojoa ke silio. Mokhoa ona o molemo ho bakang barekisi o fanang ka litšebeletso tsa boipelaetso, kaha o etsa bonnete ba ho nepahala ha protheine le sebopeho sa protheine.

Monate o Khanyang Lihlahla tse Kholohang

Ho Bula ka ho Fetoha ka Thōko e phahameng, Joaloka Trtepsin, li khahliloe ka lebaka la bokhoni ba tsona ba ho hlahisa likhechana tse boletsoeng esale pele le tse laolang. Ho fapana le moo, likeletso tse pharaletseng tse pharalletseng li felletse ka li-motsoako o rarahaneng, li tsamaisana le tlhaiso-leseling. Li-freetoes tse hlahisang li-enzyme bakeng sa lipatlisiso tsa lipatlisiso ho fihlela liphapang tsena ho fihlela litlhoko tsa mesebetsi e khethehileng ea lithupelo tse fapaneng.

Karolo ea Zymogens ho ts'ebetso ea protheste

Proteasen precortivation

Hangata litekanyetso li fana ka khatello ea liketsahalo tsa Zymogans ho thibela timetso e sa batleheng ea liprotheine tse sa rateheng. Filesinogen, e qalileng ka mpeng e nyane, e bontša mohopolo ona hantle. Ho etsa lintho tse utloisisang zymogen ho bohlokoa bakeng sa litheolelo tsa litheolelo bakeng sa ts'ebeliso ea kalafo, e netefatsa hore lingane tse laoloang ke li-enzyme.

Ho busoa ka molao ka enterokikise

Enterokikise e bapala karolo ea bohlokoa ts'ebetsong ea Trypstinogy, tiisa bohlokoa ba molao o hlakileng ho profesetion ts'ebetsong ea Profetication. Ho leka-lekana pakeng tsa Zenmone Syntherthesis le ts'ebetsong e tiisa hore ts'ebetso e itekanetseng e hlaha ka botlalo ka 'mele, taelo ea povat bakeng sa barekisi ba kenella tsamaisong ea enzyme.

Melapo ea lithibelo le mesebetsi ea bona ea likokoana-hloko

Taolo ea Ts'ebetso ea Maprosttic

Ho ipelaetsa ho lithibelo tsa lithibelo li bohlokoa ho laola tšebetso ea prothestic ka litsamaiso tsa likokoana-hloko. Ba thibela ho nyonyeha ha liprotheine tse sa laoloang, ho bohlokoa ho feta tekano ho likopo tse phahameng. Ka kalafo ea selefouno, ho utloisisa teka-tekano ho bohlokoa bakeng sa ho ba le kalafo tse ntseng li eketseha tse mamellang ho itšireletsa mosebetsing ka nepo.

Protease lithibelo ho Therapeutics

Protease bashebelli feela ba nang le likarolo tsa molao feela empa hape le likopahe tsa thekhopoic. Li fana ka melemo e nepahetseng, e kang ho thibela tšenyo ea li-a lithibela-mafu kapa ho fokotsa boima ba lithibela-mafu, ho bonts'a bohlokoa ba bona lipatlisisong tsa bongaka le ntlafatso ea bona.

Lits'ebetso tsa indasteri le li-biotechnological tsa TryPsin

Karolo ho Biotechnology

Trypsin e sebelisoa haholo ts'ebetsong ea biotechological, e joalo e le tšilo ea lijo le ho feto-fetoha ha protein. Taba ea eona le ts'ebetso ea eona e etsa khetho e ratoang bakeng sa lits'ebeliso tse ngata tsa lits'ebeliso tse hlahisang lihlahisoa tsa phekolo ea seli le moo.

Bluekit Fana ka litharollo

Bluekit e fana ka tharollo e phethahetseng e tsamaisang le litlhoko tsa protede tsa lipatlisiso le lits'ebetso tsa indasteri. Ka ho fana ka Phahameng - libaka tsa boleng ba libaka, Bluekit e tiisa hore li-inshoratories le lifetheri li na le lisebelisoa tse hlokahalang tsa lithereke tsa liprotheine tsa limmapa tsa sele, le biotechnology. Boitlamo ba rona ho boleng le boitlamo ba rona re le morekisi ea etellang pele, ho u thusa ho fihlela litholoana tsa hau tse nepahetseng tsa mahlale.