Kenyelletso
Li-gection ea Genomic ke mokhoa oa motheo oa moleology, o bapalang karolo ea bohlokoa lipatlisisong tse fapaneng tsa lipatlisiso le likopo tsa bongaka. Nts'etsopele eaGenotic DNA Extection KitS e fetotse ts'ebetso ena, e e etsa e ka etsahala, e sebetsang hantle ebile e ea tšepahala. Sengoliloeng sena se fetela ho tlhoekiso ea Genomic DNA, ho hlahloba mefuta e fapaneng ea sampole, lits'ebetso tsa tlhoekiso, mathata Khatiso e Khethehileng e behiloe bohlokoa ba bohlokoa ba Cell Thepa ea Lekhetho la Cell Therapy DNA Extlong. Hore na u mofuputsi, ngaka ea laboratori kapa laboratori, tataiso ena ea laboratori, tataiso ena e akaretsang e tla fana ka leseli la bohlokoa ka thata le tsoelo-peleng tsa Jonase ea Dna.
Kenyelletso ea lisebelisoa tsa ho hloekisoa ha DNA
● Kakaretso ea Kits ea Phano ea DNA
Li-kits tsa li-DNA tsa DNA tsa DNA tsa DNA ke lisebelisoa tsa bohlokoa ho lisebelisoa tsa bohlokoa tsa biology tsa bioloratory. Li-kits tsa kits li nolofatsa ts'ebetso ea ho tlosa, ho bolela Phahameng DNA - DNA ea boleng bo fumaneha ka har'a mofuta o fapaneng oa mohlala. Li fana ka protocol e lekanang, e fokotsang ho feto-fetoha le ho ntlafatsa tlhahiso. Fumaneha lifofotsing tse ngata, li-cater tse ngata ho mehlala e fapaneng ea mohlala le ho kenya litlhoko.
● Bohlokoa ka likopo tse fapaneng
Li-kits tsa DNA tsa ho itlhoekisa ha li sebetse, tlhahlobo ea bongaka, baoki le lithuto tsa tikoloho. Ha ba etsa lipatlisiso, ba nolofalletsa ho ithuta ka liphatsa tsa lefutso, polelo ea Gene le liphetoho tsa Eigenetic. Maemong a Clinical, li sebelisoa bakeng sa ho khetholla mafu a liphatsa tsa lefutso, ho atleha ho bonahatsa katleho ea tšoaro ea liphatsa tsa kalafo, le ho ntlafatsa moriana o iketsang. Ho tšepahala le katleho ea lisekele tsena li bohlokoa haholo bakeng sa ho hlahisa litholoana tse nepahetseng le tse khothalletsoang.
Mefuta ea disampole bakeng sa ho itšehla thajana
● Lisele tse tšoeu tsa mali
DNA ea Genomic e ka nkoa e le lisele tse tšoeu tsa mali tse sebelisang li-kits tse ikhethang. Sena se na le thuso haholonong ea bongaka le tlhahlobo ea lefutso, moo mehlala ea mali e bokelloang hangata.
● Lisele tsa moetlo lisele
Litso tsa setso sa lisele li fana ka tikoloho e laoloang bakeng sa ho ithuta boitsebiso ba lefutso. DNA EXCTION TENG LINAKENG TSE LINTHO TSE BONANG LI BUISETSA HO ETSA LIHLOOHO TSE LING TSE LING. Linotsi tse etselitsoeng bakeng sa lisele tsa setso sa lisele li tiisa chai e phahameng le bohloeki.
● Bophelo le lisele tsa lema
Ho tlosa DNA ho tsoa ho phoofolo le limela ho etsa hore mathata a ikhethang ka lebaka la boteng ba bafetoheli le meaho e thata ea selefouno. Li-kits tse ikhethang tsa DNA li ntšoaretsoe ho sebetsana le thata li thata, li bonts'a ngangisano le tlhoekiso.
● tomoso le libaktheria
Tomoso le lisele tsa baktheria li na le likamore tse hloekileng tsa lisele tse hlokang mekhoa e tobileng ea LNA e sebetsang. Li-kits tse tsamaisang likokoana-hloko tsena li sebelisa mekhoa e metle ea ho lokolla ea ho lokolla e phahameng - DNA ea liketsahalo tsa boleng bo holimo.
Mohato - ka -
● Litokisetso tsa pele tsa mohlala
Mohato oa pele oa tlhophiso ea DNA o kenyelletsa ho bokella le ho lokisa sampole. Sena se ka kenyelletsa ho hlatsuoa, ho hlatsoa le ho hlatsoa le ho fehla ho phahamisoa ho latela mofuta oa sampole. Boitokisetso bo nepahetseng ba sampole ho bohlokoa bakeng sa ho fokotsa tlhahiso ea DNA le boleng.
● Lysis le mekhabiso e tlamang
Sethala sa ho rata se kenyelletsa ho roba lisele tsa sele le ho lokolla lefu la ge ka tharollo. Sena se fihlella ho sebelisa li-sekhobo, li-enzyme, kapa mekhoa ea ho fana ka maikutlo. Mehato e tlamang e latelang, moo DNA o tlangoa ka mokhoa o ikhethileng, joalo ka silica, tlasa maemo a letsoai le le boima.
● Mehato ea ho hlatsoa le ho hlapa
Mehato ea ho hlatsoa ho tlosa singlents le lipelaelo, li bolela ho hloeka ha DNA. Li-buffers tse ngata li sebelisoa ho felisa liprotheine, lipID, le maloanlahla a mang a cellular. Qetellong, mohato oa ho bolaea o lokolla DNA e hloekisitsoeng ho tloha mothating o tiileng o sebelisang sethunya kapa metsi a tlase.
Tharollo - Mekhoa e Thehileng bakeng sa ho itšehla thajana
● Ho bapisoa le mekhoa e meng
Tharollo - Mokhoa o Thehiloe ka Pherekano, e kang Phenol - Ts'ebetso ea Phlenol - Ts'ebetso ea Phlenol - Ts'ebetso ea Phlorol Le ha a ntse a sebetsa, ba baroetsana - ba matla ebile ba hloka lik'hemik'hale tse kotsi. Ho fapana le moo, li-dana tsa mehleng ena tsa DNA li sa sireletseha, kapele, le ho feta ho sebelisa hape ho na le mekhoa e metle.
● melemo ea tharollo - mokhoa o thehiloeng
TLHOKOMELISO Li etselitsoe ho fokotsa litšila mme li ka boleloa habonolo bakeng sa Phahameng - Likopo tse phahameng. Ho feta moo, ha ba hloke lik'hemik'hale tse kotsi, li ba etsa ba bolokehile bakeng sa basebetsi ba laboratori.
Liphephetso ho Phano ea DNA
● Litaba tse tloaelehileng tse neng li kenella
Ho itlhomafatsa DNA ho ka itlhoekisiti ka mathata, joalo ka lihlahisoa tse tlase, litšila, le nyollo. Litlhophiso tse teng ho sampole li ka kena-kenana le lits'ebeliso tse nang le lits'ebetso tse tlase, 'me li sebetsana hampe ka nepo li ka fella ka lefu.
● Malebela a ho rarolla mathata
Ho sebetsana le mathata ana, ho bohlokoa haholo ho latela protocol ea kit hantle. U sebelisa likhopo tse ncha, ho boloka tikoloho e hloekileng ea ho sebetsa, 'me ho netefatsa litokelo tsa mehlala e mengata ho ka fokotsa mathata a mangata. Ntle le moo, ho kenyelletsa mehato ea bohlokoa, e kang ho lekanya DNA le bohloeki, ho ka thusa ho tseba le ho rarolla mathata kapele.
Likopo tsa lipatlisiso le tsa bongaka
● Sebelisa lipatlisiso tsa liphatsa tsa lefutso
Linotlolo tsa geniolic li nne litlatsetso tsa liphallelo, li thusa lithuto tsa liphallelo, e nolofalletsang lithuto tse atlehileng le tšebelisano. E ts'ehetsa mekhoa e kang pcr, e lekanyetsang, le ea bohlokoa, e leng lintho tsa bohlokoa ho utloisisa mekhoa ea liphallelo.
● Likopo ka li-diasical tsa bongaka
Ka li-diabolostics tsa bongaka, - DNA ea boleng bo phahameng e bohlokoa bakeng sa bokuli bo nepahetseng ba ho fumana boitšisino, botsitso le ho hlokomela kalafo. Likopo li kenyelletsa tlhahlobo ea lefutso bakeng sa mathata a futsitsoeng, makhooa a mofets'e le a tšoaetsanoang mafu a tšoaetsanoang. Ho tšepahala ha li-kits tsa DNA li le bohlokoa bakeng sa ho fana ka tlhaiso-leseling e nepahetseng le e nepahetseng.
Boleng le chai ea DNA e hloekisitsoeng
● Ke lintlha tse amang boleng ba DNA
Ho na le lintlha tse 'maloa tse susumetsang boleng le chai ea DNA e hloekisitsoeng, ho kopanyelletsa le mofuta oa sampole, mokhoa oa ho tlosa sampole, le lits'ebetso tsa ho tlosa le ho sebetsana le lits'ebetso tsa ho tlosa. Ho ba teng ha bafetoheli, joalo ka liprotheine le polysaccharides, li ka ama lits'ebetso tse theotsoeng.
● Mekhoa ea ho lekanya bohloeki ba DNA le ho tsepamisa mohopolo
Ho lekola bohloeki le ho tsepamisa maikutlo ha DNA ho bohlokoa bakeng sa ho netefatsa tlhokahalo ea lits'ebetso tsa tlase. Mekhoa e tloaelehileng e kenyelletsa tlhahlobo ea sephiri, e fanang ka liteishene (A260 / A280) ho lekola bohloeki, bo fanang ka litekanyo tse tebileng le tse nepahetseng.
PUSELETSO LE LITLHAKISO TSE KHOLO TSE KHOLO TSE KHOLO TSE KHOLO
● Masene a kenyelletsa mekhoa e ikemetseng e ikemetseng
Ho hloekisoa ha DNA ho ntse ho le bohlokoa haholo hore re tšoase lipalo tse ngata tsa mohlala le ho ntlafatsa mosebetsi oa matsoho. Litsamaiso tse ikemetseng tse kang li-roobit tsa liroboto le lijalo tsa liroboto tsa roboto tsa kobo, li tsamaisana le bokhabane, li fokotsa phoso ea motho le ho eketsa tlhahiso.
● Melemo ea tharollo e phahameng ea ho kenella
Litharollo tse phahameng tsa DNA tsa PH li bohlokoa bakeng sa tse kholo - Ba nolofalletsa ts'ebetso e ts'oanang ea li-sampole tse ngata, ho boloka nako le lisebelisoa. Litsamaiso tse ikemetseng le tsona li fana ka litholoana tse sa fetoheng, ho ntlafatsa tlhahiso le ho ts'epahala.
Tlhahlobo e bapisang ea li-kits tsa khoebo
● Papiso ea tšebetso ea kite tse tummeng
Ho na le lisebelisoa tse ngata tsa DNA tsa DNA tsa DNA tse fumanehang ho baetsi ba sa tšoaneng, e 'ngoe le e' ngoe e na le likarolo tse fapaneng le litšobotsi tsa ts'ebetso. Lintlha tseo re lokelang ho nahana ha li khetha sehlahisoa, bohloeki, ho itlhoekisa ha ho sa khone ho sebelisoa, 'me u litšenyehelo. Lithuto tsa lipapatso le litlhahlobo tsa basebelisi li ka fana ka leseli la bohlokoa karolong ea lisebelisoa tse fapaneng.
● Maikutlo a bareki le litsebi tsa litsebi
Tlhahlobo ea karabo ea mosebelisi le lipatlisiso tsa setsebi li bapala karolo ea bohlokoa ho hlahloba katleho ea li-dna tse kenyelletsang tsa Geomitic DNA. Litlhahlobo tse ntle le liphello tse tsoang mehloling e tummeng li ka tataisa barekisi ba ho khetha litlhoko tse ntle ka ho fetisisa bakeng sa litlhoko tsa bona. Ntle le moo, maikutlo ho basebelisi ho thusa baetsi bahlahisi ho ntlafatsa lihlahisoa tsa bona le ho rarolla mathata afe kapa afe.
Mekhoa ea nako e tlang ho Theknoloji ea Pusna ea Phano
● Maangolong a hlasimolohang a DNA Extloction
Tšimo ea tlhoekiso ea DNA e ntse e fetoha hantle, ka mahlale a macha a hlaha ho sebetsana le mefokolo ea hajoale. Litsebi tse kang tsa Microfluidics, Nancotochnology
● Likhariso mabapi le tsoelo-pele ea nakong e tlang ea tšimo
Mekhoa ea bokamoso e fantisi ea DNA e kanna ea tsepamisa maikutlo ho methapo, menriaturization le ho hokahana le mekhoa e meng ea biogy ea biology. Nts'etsopele ea li-kits tsa bokahohle tse khonang ho sebetsana le mefuta ea mohlala le maemo a lebelletsoeng ho ntlafatsa maemo le maemo a ho feto-fetoha ha maemo
Bluekit: DNA EXTREPILLINEYALO
Jangsu Hillgene, tlasa lebitso la mofuta oa lebitsoBluekit, ipehile e le sebapali se etellang pele lebaleng la Genomic Alctions. Lebopong la Suzhou, le libakeng tsa ho etsa lihlahisoa tsa Shenzhen le Shanghai le Shanghai, le Hillgene Lihlahisoa tsa Bluekiti li etselitsoe ho kopana le litlhoko tse thata tsa kalafo ea cellular, ho tšehetsa nts'etsopele ea koloi - Ka boitlamo ho ntlafatso le boleng, Hillgene e ikemiselitse ho ntšetsa tšimo ea bongaka le ho ntlafatsa sephetho sa mokuli.
Ka kakaretso, tlhoekiso ea Genomitic DNA ke ketsahalo ea bohlokoa ho biology ea molek'hupelo, e nang le likopi tse ngata - Tsoelo-pele e maemong a DNAITIONS, ho kenyeletsa le tse blukit, li entse hore mokhoa ona o sebetse hantle hape o ka ba a sebetsa. E le theknoloji e ntse e tsoela pele, bokamoso ba tlhoekiso ea DNA bo na le menyetla e metle ea ho ntlafatsa phaello ea saense le boqapi ba kliniki.
Nako ea poso: 2024 - 09 - 27 14:40:06
