Kenyelletso
Tšimo ea Thekolo ea Sebele e bone esale pele ka mokhoa o hlollang lilemong tse leshome tse fetileng, haholo-holo katolosong le lisele tsa tlhaho. Lisele tsena tsa 'limm tse ke keng tsa bapala karolo ea bohlokoahali molemong oa ts'ireletso ea' mele, e se e le ntho ea bohlokoahali ho hlaolela lefu la lits'oants'o le maloetse a mang. Sengoliloeng sena se fetela ka ho itlotsa ka thata ka lisele tsa katse tsa NK Cell, karolo ea Tšobolast le sengoliloeng sa K562, bohlokoa ba li-cytokines le mekhoa e tlang ea phekolo ea NK. Ntle le moo, re ama bohlokoa baDNA ea DNA ea Cele Preptoodding Kits ka ho netefatsa boleng le polokeho ea lihlahisoa tsa kalafo ea libapali tsa Ceop.
Kakaretso ea Kits ea Kholo ea NK Cell
● Bohlokoa ho kalafo ea seli
NK Cell Cell Cell Hole Ke Litopo tse Thabisang Boemong Sebakeng sa The Wilding, E thusa ho phekola lisele tsa NK ho Therapeutic maemong a Therapeutic. Kamanong e mengata ea likarolo tsa lisele tse ngata tse lekaneng tsa NK e hloka likopo tsa bongaka, ka tsela eo, khothaletsa bokhoni ba bona ba kalafo.
● Likarolo tsa Kit
Ka tloaelo, lisekele tsa kereko tse kholo tse nang le lisele tse kenyeletsang lisele tsa bapeer, li-cytokines le cel bal rasal e khethehileng. Likarolo tsena li sebetsa li-syneristally ho theha tikoloho e matla bakeng sa sebaka sa cell celllication le ts'ebetso ea li-celliferation le ts'ebetsong, e hloekisang.
Karolo ea lisele tsa Trophoblast ho ts'ebetso ea NK
● Mochini oa ho khothatsa
Lisele tsa Trophoblast ka tlhaho li sebelisana le lisele tsa NK nakong ea kema, ho bapala karolo ea bohlokoa ea ho susumetsa ts'ebetso ea tsona. Maemong a keketseho ea cell cell, lisele tsa Trophoblast li ka tsosa lisele tsa NK ho theosa le lisele tsa NK ho theosa le tšebetso ea bona ea kou.
● Melemo ea ho sebelisa lisele tsa Trophobllast
Ho sebelisa lisele tsa Trophoblast ho NK Cell ka mokhoa oa 'mele o fana ka mokhoa o matla oa ho tsosa lisele tsa NK, li ka e hlahisa ho lisele tse nang le ts'ebetso e phahameng.
Moenjiniere K562 lisele tse katolosoang ea sele ea NK
● Ho boleloa ha lisele tsa Cytokine ho K562
Lisele tsa K562 li sebelisoa hangata e le lisele tsa li-bapere tsa li-NK Cell. Li fetotsoe ho hlahisa li-cytokines tse kang IL - 21 le IL - 21, e leng bohlokoa bakeng sa kholo ea NKT le ts'ebetso.
● Sesebelisoa sa ho se tsotelle le ho se sebetse
Ho se nke sebaka sa lisele tsa K562 pele ho sebelisoa ha li-speage tse sa batleheng, ha a ntse a boloka bokhoni ba bona ba ho tšehetsa kholo ea sele ea NK.
Bohlokoa ba li-cytokines ka ts'ebetso ea NK
● IL - ho ba le litlamorao tsa 21 le litlamorao
Li-cytokines li ka - 21 e ba bohlokoa ho kenya tšebetsong ts'ebetso ea celts. Il -
● mekhoa ea matšoao a matšoao
Li-cytokines li sebetsa litseleng tse fapaneng tsa pontšo ho kenya lisele tsa NK. Ho utloisisa litsela tsena ho bohlokoa bakeng sa ho ntlafatsa li-protocol tsa kalleng ke NK Cell ka katleho le ho ntlafatsa katleho ea tsona ea kalafo.
Mehloli ea Cell ea NK: NUMBILICE
● Papiso ea lihlahisoa tsa sele
Mali a thapo ea thapo ea mongobo ke mohloli o motle oa lisele tsa NK, hangata o hlahisoang ke linomoro tse phahameng ha ho bapisoa le mali a phahameng. Leha ho le joalo, khetho e pakeng tsa mehloli ena e latela litlhoko tse ikhethang tsa kopo ea kalafo.
● Ho hloeka le boleng ba lisele tse kholo tsa NK
Mehloli e ka 'ngoe e ka fa PK ea NG - Lisele tsa NK tsa NK, empa li-protocol tsa keketseho li ka fapana. Ho netefatsa bohloeki le ts'ebetso ea lisele tse atolositsoeng oa NK e eketsehang tsa NK.
Kopo ka koloi - Boitokisetso sa sele ea NK
● Mekhoa ea ho etsa koloi ea ho tiisa koloi - lisele tsa NK
Car - Lisele tsa NK li bolokiloe ho bua ka lisoto tsa li-antigen Antigen, li ba nolofalletsa ho lebisetsa lisele tse khethehileng tsa mofets'e. Ho hlophisoa ha koloi - Lisele tsa NK li ama phetoho ea liphatsa tsa meno le katoloso, lits'ebetso tse hlophisitsoeng ke li-kits tse ikhethang.
● Melemo e fetang mekhoa ea setso
Car - Lisele tsa NK li fana ka melemo e meholo ea graft - Harsus - Ho aha Maloetse a Khoebo le Maloetse a Khoebo le Bokhoni ba ho Shebella Maloetse a Antiger ea mofets'e.
Ts'ebetso nts'etsopele ea The Cell Therapy
● Nako le katleho e atlehileng nts'etsopele ea pele
Ho hlaolela mekhoa e nepahetseng bakeng sa kalafo ea NK Celepy ke ea bohlokoa bakeng sa nako le litšenyehelo methating ea khale ea khale. Ho ntlafatsa protocol le ho nyekeloa hantle - ho - Sebelisa li-kits tsa katoloso li ka tsamaisana le ts'ebetso ena haholo.
● Maeto a ho ntlafatsa lihlahisoa tsa ho phekola tsa Cell
Maano a kang atotions, ho sebetsa ka mokhoa o koetsoeng, le tšebeliso ea likokoana-hloko - Libaka tsa mahala li bohlokoa bakeng sa ho ntlafatsa boleng le lihlahisoa tsa bongaka tsa Cell Therapy.
Ho ntlafatsa bohloeki le tholoana ea lisele tsa NK
● Mekhoa ea ho fihlela bohloeki bo phahameng
Mekhoa e kang lipalesa tsa lipalesa - Karohano ea Bead e sebetsa ho fihlela maemo a holimo a bohloka ea bohloeki ho lisele tsa ho atolosoa bakeng sa lits'ebetso tse kholo tsa NK.
● Liphephetso le tharollo ho kholo - tekanyo e kholo
Ho qhekella ka mokhoa o kholo ho beha mathata a kang ho boloka lisele le tšebetso ea sele. Moralo oa BioreCtor o thehiloe le lits'ebetso tse tsoelang pele tsa tumello ea tlhokomeliso ke tharollo maemong ana.
Melemo ea ho sebelisa Cell Bal Bal Medium
● Karolo ea katoloso ea sele le ts'ebetso
NK Cell Basal Medium Medum e hlophisitsoeng ka kotloloho ho ts'ehetsa kholo le ts'ebetso ea lisele tsa NK, li bolela tšebetso e sa fetoheng ho potoloha li-protocols tse fapaneng.
● Ho tsamaisana le li-cytokines tse fapaneng
Kamohelo ea bohareng e nang le li-cytokines e lumella ho fetoha le maemo ka keketseho ea cell cell ka cell ho latela litlhoko tse ikhethang tsa kalafo.
Likabelo tsa nako e tlang tsa kalafo ea Nk
● Litekanyetso tse hlahelang tsa The Cell Therapy
Tšimo ea kalafo ea NK Celd Refiy e fetoha matla ka potlako, ka mekhoa e hlahang e shebile ho matlafatsa mahlomola, e fokotsa litlamorao tsa kalafo.
● Ke tsoelo-pele ea likopo tsa NK
Tsoelo-pele ea nako e tlang e ka kenyelletsa ntlafatso ea lisele tsa Universal NK, kalafo ea lisele tse ling tsa lisele tse ling tsa NK.
Ho netefatsa boleng ka li-dna tsa li-dana tsa lisele tse sa pheleng
● Bohlokoa ho kalafo ea seli
Li-kits tsa li-cele tsa sele li sa sebetse hantle ka litsing tsa sele bakeng sa ho bona le ho kenyelletsa DNA e setseng ea DNA. Li-kits tsena li tiisa polokeho le katleho ea lihlahisoa tsa kalafo ea sele ea Cell kalafo ka ho fokotsa kotsi ea tšilafalo.
● Karolo ea Baetsi le barekisi
Baetsi le barekisi ba li-dana tsa lisele tse neng li sa sebetse hantle, joalo kaBluekit, re bapale karolo ea bohlokoa ho fana ka tšepiso le e phahameng - Li-kits tsa boleng. Li-kitsi tsena li bohlokoa bakeng sa ho boloka botšepehi ba tlhahiso ea lisele tsa ho iphelisa.
Sephetho
Sebaka sena sa kalafo ea sele ea sele ea sele, ka lisele tsa NK li ka peletoeng ea phetohelo ena. Khokahano ea li-kits tsa keketseho e tsoetseng pele, litekanyetso Ha indasteri e ntse e tsoela pele, tšebelisano lipakeng tsa bafuputsi, li-clinicas le bahlahisi li tla ba senotlolo sa ho senola bokhoni bo felletseng ba phepo e felletseng ea phepo ea NK.
Jiangsu Hillgene, tlasa Bra Bluekit®, e eme pele ho pele ho moo e nchafatsoang. Ka ntlo ea eona libakeng tsa Suzhou le libaka tsa ho etsa lihlahisoa tsa China le North Karolina, Hillgene ke li-plays tsa theko ea nyutlelie. E etselitsoe taolo ea boleng, lihlahisoa tsa Bluekit® li tiisa polokeho le katleho ea kalafo ea 'mele, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, T, t, Ka pono ea tšusumetso ea kalafo ea li-celli tsa 'mele, Hillgene e ikemiselitse ho ntšetsa pele kalafo ea liliba ea selimahale lefatšeng ka bophara.
Nako ea poso: 2024 - 12 - 13:31:09
