Sebakeng sa lipatlisiso tsa biondical le nts'etsopele ea kalafo ea kalafo, li-enzyme tsa phepelo li bapala karolo ea bohlokoa. Har'a tsena, ho ke ke ho itšepa thahasello ea thahasello e kholo, e keteketsoe bakeng sa ho hlaka ha eona ho tlamahana pekisite e hloekisang. Sengoliloeng sena se fetela pele karolo ea bohlokoa ea li-trypsin bakeng sa lipatlisiso tsa biodisi, mefokolo ea mekhoa ea setso, mefokolo e kholo ea Techsin ea ho fumanoa e le mahlale, le joang BluekitTrypsin kitS li fetolela tšimo.
1. Bohlokoa ba Trypsin Patlisisong ea Biomedical
1.1 Karolo ho Cli ho Fola
Trypsin ke ea bohlokoa molemong oa ho feto-fetoha ha lifola, mokhoa oa bohlokoa ho litso tsa sele. Ka ho theola liprotheine tse khothatsang khokahano ea sell, Trypsin e lumella ho phethahala ha mefuta ea sele kapa ho thusa lits'ebetsong tse kang kalafo ea selefouluoa le moriana oa ho nchafala.
1.2 Likopo tsa TEPESITION
Ntle le ho fetoha ha sele, bokhoni ba Fanpsin ho etsa hore lithepe tse bohlokoa li etsa hore ho be le bohlokoa ho tse tebileng tsa li-speprometry - Ts'ebetso ena ea boikhathollo e etsa hore bafuputsi ba sekaseka mekhatlo ea protheine, 'me ba hlahloba litšebelisano tsa profetice, tseo kaofela ba bohlokoa bakeng sa kutloisiso ea limolek'hule.
Monehelo oa 1.3 ho nts'etsopele ea Therapeutic
Nts'etsopele ea Therapeutic, Fispsin e sebelisoa ka har'a liprotheine - Ho kenyelletsa li-antibodies tsa li-antibodies le liprotheine tse nchafatsang. Melemo ea eona e hlakileng e hlakileng e tiisa ts'ebetso e nepahetseng ea liprotheine tse nepahetseng, li hatisa bohlokoa ba eona ho tlhahiso ea bidopprics.
2. Meeli ea libaka tsa setso tsa Trypsin
2.1 Litaba tse nang le kutloelo-bohloko
Li-tristen tsa setso li na le litaba tsa maikutlo li na le litaba tsa maikutlo, li fokotsa bokhoni ba bona ba ho bona maemo a tlase a ts'ebetso ea li-tryPsin. Sena se ka sitisa ho shebella le ho khethoa likopang tse fapaneng, ho etsa phephetso libakeng tsa bona tsa lipatlisiso le lits'ebetso tsa indasteri le lits'ebetso tsa indasteri.
2.2 Nako - Litsamaiso tsa ho ja
Mekhoa e Ikemetseng ea ho hopola mesebetsi e tloaelehileng ea Horce Mekhoa ena ea majoe e ka liehisa linako tsa liteko tsa liteko ebile li eketsa kotsi ea liphoso, haholo-holo ho libaka tse phahameng - Litaba tsa thuto.
2.3 Kotsi ea Cross - Boikoetliso
Cross - Sena se etsa hore ho ts'epahale ho tšepahala ha litholoana ebile ho ka etsa hore ba qete lipatlisisong tsa mahlale.
3. Liphetoho tsa Trypsin Trypsin Expression
Tlhahlobo ea 3.1
Tsoelo-pele ea morao-rao ea theknoloji ea Trypsin e fumanoeng e le holimo - Li-antibodies tsena li enjellotsoe ho tlanya ka leeme ho trypsin, e fokotsang bocha le ho nolofatsa palo e nepahetseng ea ho lekanngoa le maemo a fapaneng a litente tse fapaneng.
3.2 E NplectB e Nolofalitsoeng le E Sebetsang
Lipolelo li lebisitse ho nts'etsopele ea lipalesa tse khaphatsehang haholo tsa ho nka nako le ho rarahana. Mekhoa ena e mecha e kopanya mosebelisi - Protocol e nang le botsoalle e tsamaisang le lits'ebetso tsa boithatelo, e tsamaisang likamano tsa bona tsa laboratoring le litlhophiso tsa indasteri.
3.3 Ho holisa maemo a matla a matla bakeng sa ho lemoha
Mehla e matla ea chelete ea sejoale-joale ea li-IRAS Bokhopo bona ke sa bohlokoa bakeng sa likopo tse hlokang ho shebella hantle, tse kang taolo ea boleng le lipatlisiso tsa prothemics tsa Kopharmas.
4. Ho ntlafatsa lipatlisiso tsa khale tsa biopharmactical le kits e ncha
4,1 Ho lekola masala a liprotheine tsa protheine
Ha u etsa hore liprotheine tsa protheine, ho bohlokoa ho shebisisa sebaka se setseng ho netefatsa polokeho le katleho. Ka tsela e tšepahalang, ka ho khetheha e etselitsoe morero ona, ka tsela e tšepahalang ha u le lihlahisoa tsa pherekano, ka ho etsa molumo o khothatsang mehato ea tiiso.
4.2 Ho ntlafatsa li-protocol tsa cellular
Thepa ea kalafo, moeli o tšepisang oa bongaka, e hloka taolo ea boleng bo boima ho fihlela litekanyetso tsa taolo ea ts'ehetso. Sebeleleng sa kalafo ea libapali sa Cell
TLHOKOMELISO EA 43 Ho netefatso ea liprothemo
Kopo ea li-la li-Indi tse tsoetseng pele tsa litempele li felletse ka ho ba le tšepo e phahameng litabeng tsa protheine le khethollo. Ka ho fana ka sephetho se nepahetseng le se khothalletsang, li-kits tsena li ntlafatsa bonnete ba data ea protheomic, e leng litaba tsa ho senyeha ha Biomarker le boits'oaro bo botle.
5. Pagetimonial ho tsoa mekhatlong e etellang pele ea lipatlisiso
LITLHAKISO TSA BOPHELO
Litsi tsa Patlisiso li hlahisitse ntlafatso ea mantlha tsamaisong ea kotulo ea Cell ea Cell ea ho sebelisa li-blupsin li-kitpsin kits. Phetoho e ntlafalitsoeng le ho nepahala ha lisene tsena li nolofalitse ho itšepa haholoanyane, ho nolofalletsa bafuputsi ba ha phetha lithuto tsa bona ka kholiseho e kholo.
5.2 PUP - Ho kena ka mokhoa oa ho hlahlobisisa
Khokahano ea li-blupsin tsa li-blupsin tsa li-bluppin tsa creapsin li fumaneha ka holimo ho Pearmation Platshing. Hona joale bafuputsi ba rua molemo bokhoni ba ho etsa se seholo -
6. Karolo ea Bluekit ka biolo ea sejoale-joale
6.1 Scaliting mekhoeng ea bioprocesting
Boqapi ba Bluekit bo khetholloang khetho ea disppen ba bapala karolo ea bohlokoa ho hohlapa mekhoa ea biopledoping. Ka ho khothaletsa taolo ea boitlamo ba enzzymatic, lisebelisoa tse ts'ehetsang ts'ehetso ea tsamaiso ea lihlahisoa, ho eketsa chai ea balolo, ho fihlela boleng le ho netefatsa boleng ba sehlahisoa.
6.2 Ho kenella ka mekhoa e metle
Khokahano ea li-blupsin tsa li-cuppin tsa Blueskit tse nang le mekhoa e ikemetseng e supa leap ho sebetsa hantle. Khokahano ena e lumella ho kenyelletsa batho ba bangata ho kenyelletsa litsebo tse teng, ho fokotsa ts'ebetso ea ho kenella le ho ntlafatsa ts'ebetso ea ho kenella.
7. Ho netefatsa tlhoekiso ea sehlahisoa ho lihlahisoa tsa GPP
7.1 Bohlokoa ba ho shebella litente tse nepahetseng tsa litente
Ka mokhoa o motle oa tlhahiso (GMP) Litlhokahalo tsa Cloppit tsa Blupkit li fana ka nepo le ho ts'epahala tse hlokahalang ho fihlela litlhoko tse thata tsa moea, sireletsa boleng le polokeho ea biopharmacticals.
Lisebelisoa tsa 7.2 bakeng sa ho boloka taolo ea boleng
Sutu ea lisebelisoa e felletseng ea lisebelisoa tse fanoeng ke baetsi ba li-bluekit e matlafatsang ba bluekit bahlahisi ba matla ho boloka litekanyetso tsa taolo ea boleng bo matla. Lisebelisoa tsena tse tsamaisang lipontšo tsa liphoso le netefatso ea ts'ebetso ea enzymatic, e bohlokoa ho tokollo e atlehileng ea ho lokolloa ha lihlahisoa tsa kalafo.
8. E lekanyetsoang ea mohlala bakeng sa meriana ea motho e mong
8.1 Hlokatso bakeng sa tlhahiso ho mokuli - mehlala e mengata
Ka meriana ea motho ea hlorisoang, hlahisa e bohlokoa haholo. Li-kits tsa li-clupskin tsa Blueskit li netefatsa hore li lula li hlophisitsoe, li lumella litekanyo tse tšepahalang ho mokuli - Sampole e Loketseng. Tekanyetso ena e bohlokoa bakeng sa tlhahlobo e nepahetseng le ho etsa tloaelo ea ho kena-kenana le phepelo ea kalafo.
8.2 Melemo ea Liphetho tse Khabane
Liphetho tse thata tse fanoang ke li-blupsin tsa li-cuppakin li fokotsa ho se tšoanele data, ho ntlafatsa ts'abo ea lipatlisiso tsa bongaka. Ho etsa seponno sena ho tšehetsa nts'etsopele ea mokuli - liphekolo tse ikhethang, ho ntšetsa pele tšepiso ea moriana o ikemetseng.
9. Litaelo tse tlang tsa nakong e tlang tsa tlhahlobo ea protepele
9.1 Ho nchafatsoa ha boputsoa ba Blukit le tšusumetso
Bluekit e ntse e tsoela pele ho etella pele ho etsa lipatlisiso mabapi le lipatlisiso tsa protepele ka ho khaola - Edge Trypsin Kits. Ka ho sebetsana le liphephetso tsa litlhaloso tsa setso le ho fana ka tharollo ea boiphihlelo, Bluekit e betla tsela ea ho fumana le likopo tsa bophelo.
9.2 Litlhoko tse mpe le liketsahalo tsa theknoloji
Ha tšimo ea protume e etsa lipatlisiso, liphephetso tse ncha le menyetla e tla hlaha. Bluekit e ikemiselitse ho khotsofatsa litlhoko tsena tse hlahang ka ho itokafatsa le boitlamo ba eona, ho netefatsa hore ho hlokahala bafuputsi.
10. Qeto ea ho ntlafatsa mahlale a hlapanya ka botona tse boputsoa
Meeli ea sebaka se tloaelehileng sa litente e le khale e le tšitiso ho ea lipatlisiso le ntlafatso ea kalafo. Leha ho le joalo, ka nako ea ho qaptjoa ha li-typsolution tsa Revolution ea Blupsit, mathata ana a ntse a felisoa. Ha moetsi oa li-IRDPSIN KIT, Bluekit ke molekane oa bohlokoa oa bophelo ba litaba, a fana ka maikutlo a ho ntlafatsa ts'ebetso, katleho le ho ts'epahala le ho ts'epahala le ho ts'epahala le ho ts'epahala.
AboutBluekit
Jiangsu Hillgene, k'hamphani e ka morao ho Bluekit, e thehile ntlo-kholo ea eona ShezHe 'me e sebetse libaka tse hlahisang katoloso North Carolina, USA. Ho ikhethela ka ntlafatso ea sehlahisoa sa celleng foelleng, Hillgene e fana ka sepala sa tlhahiso ea nyutledic acid le serum - mahala mahala. Tsoelo-pele ea bona ea theknoloji e tšehetsa ntlafatso ea koloi - T, T, T, T, le lihlahisoa tsa sele ea stem. E etselitsoe taolo ea boleng, letoto la Bluekit le tsamaisa papatso ea phekolo ea lisele, e rua molemo bakuli bakuli lefatšeng ka bophara.
Nako ea poso: 2025 - 04 - 09 11:47:04
