Pichia Pastoris HCP (Host Cell Protein) Resarao Kuwanikwa Kit

Pichia pastoris HCP (Host Cell Protein) Residual Detection Kit

Pichia Pastoris HCP (Host Cell Protein) Resarao Kuwanikwa Kit

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✓ Mumwe - Nhanho Elisa Protocol - inochengetedza nguva (<2h yakazara nguva nguva)
✓ Pichia Pastoris - Zvakananga - Hapana Cross - Kugadzirisa neCho / HCPS
✓ Kugadzirira - To - Shandisa - Hapana Kufungidzira Kwekufungidzira Kunodiwa

Nhamba

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Wedzera kune ngoro

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Kuongorora

Protocols

Zvinyorwa

Kutumira & Kudzoka

Vhidhiyo kurekodha

Yakatarisirwa kushandiswa
Iyi kit yakagadzirirwa kuwanda kwekuona kweiyo host cell protein (HCP) zvasara muBiolipreutal zvigadzirwa zvinobva Pichia Pastoriis Starins (i.e., GS115, x33), kushandisa imwe - STEP NGUMBEE - LINESED Impunororort Assay (Elisa). Yekutsvaga kushandiswa chete. Kwete nekuda kwehunyanzvi.

Nheyo yekuona
Iyo kit inoshandisa a Sandwich Elisa Nzira:

Kuunganidza: Microplate madime ari pre - akabatanidzwa nemakwai polyclonal antibodies kupokana Pichia Pastoriis HCPS.
Incubation.Pichia Pastoriis Hcp antibodies.
Kuwanikwa: Mushure mekugeza, tmb substrate inowedzerwa kune ruvara rwevaraidzo. Kuita kwacho kunomira, uye kusanzwisisika kunoyerwa 450 NM.
Kuwanda.

ZVINOKOSHA ZVINOKOSHA

Hapana Kufungidzira Kwekufungidzira Kunodiwa (chete dhiliation yekushandisa inodiwa).
Yakatorwa protocol: Matanho mashoma, nekukurumidza mhinduro (<2 maawa).
Yakakwirira chaiyo uye kuvimbika: Optimised for Pichia Pastoriis HCPS.

Nhoroondo dzehunyanzvi

Inoenderana samples: Tsika yeCell Supernatants, yakachenesa zvipenyu.
Dynamic range: 1-100 ng / ml (yakafanana).
Regulatory Alignment: Inokodzera kuita danho rekuvandudza uye GMP Lest kuburitsa kuongororwa.

Kushandiswa Kuita

1. Kuenzana
- Uyai nezvinhu zvese kumhepo tembiricha (≥20 min).
2. Sampu / Anti porston
- Wedzera samples / calibrators: 100 μL / zvakanaka
- Wedzera 1x enzyme - yakabatana antibody: 100 μL / Zvakanaka (ona Legend ①)
3. Incubation
- Incubate kuRT, yakachengetedzwa kubva muchiedza, 600 rpm ye 3 h (ona Legend ③)
4. Kugeza
- Geza 1: 300 μL / ell 1x buffer (vertical plate chinzvimbo)
- Wash 2 - 5: 300 μL / ell 1x buffer, 5 mashambadziro
- Pat yakaoma.
5. TMB Substrate Development
- Wedzera TMB: 100 μL / zvakanaka
- Kukudziridza: 10 min ku rt (yakachengetedzwa kubva pachiedza)
6. STOP Reaction & Reaout
- Wedzera STP Solution: 50 μL / Zvakanaka
- Mira: 5 min
- Verenga: Od at 450 NM (Reference 620 NM)

Chii chiri pabhokisi?

Chikamu	Chigadzirwa Nha.	Kutaurwa	Tsananguro
Pre - yakachekwa anti - Pichia HCP microplate	PSA015	8 Wetsime】 12 mitsetse	Pre - Yakarongedzwa neGwai Polyclonal Anti -Pichia Pastoriis Hcp antibodies. Yakaiswa chisimbiso muhombodo inoba nedunhu. Chitoro chakachengetedzwa kubva pachiedza.
Pichia HCP Calribrator	Pnb015	2 vials	Lyofililly poda. Regedza ne 500 μl calriar DILUEUT (PNC002). Bvumira 5-10 min zvekunyunguduka (mhinduro inofanira kunge yakajeka). Chitoro chakachengetedzwa kubva pachiedza.
Calriar Duliuent	PNC002	1.5 ml × 1 tube	Yakajeka mhinduro yekuvandudza pichia hcp calibrator.
Assay Duliuent	Pne006	25 ml × 2 mabhodhoro	Kune vakadzika calating, samples, uye enzyme - inobatanidza antibody. Inosimbisa yakakwana dhifizi dhifiti yezvinhu zvitsva samples.
Geza Buffer (10 × ongorora)	PNF001	25 ml × 2 mabhodhoro	Yekugeza plat. Makristasi anogona kuumba pakudziya kwakadzika-makungwa pa37 ° C usati washandiswa. Dilute 10 × nehurefu mvura.
HRP - Conjugated Anti - Pichia HCP Antibody (100 ×)	PNN009	120 μL × 1 tube	Makwai ezvemakwai antibody. Dilute 100 × neAnay Diliuent usati washandisa. Chitoro chakachengetedzwa kubva pachiedza.
TMB Substrate	PND004	12 ml × 1 bhodhoro	Kuenzana kumhepo tembiricha (≥20 min) usati washandiswa. Chitoro chakachengetedzwa kubva pachiedza.
Regedza mhinduro	PNNI002	6 mL × 1 bhodhoro	Ine HCL. Pfeka goggles uye dzivisa kusangana kweganda.
Kuongorora Mafirimu	Pnk001	3 mashitsi	Yekuvhara micrate micrate kudzivisa kusvibiswa uye kubuda kwemvura.

BlueKitbio yakazvipira kupa zvigadzirwa zvakanakisa muindasitiri yekutsvaga yekutsvaga.

Kuendesa ruzivo

Isu tinopa firiji kutakura pane ese mairairo. Kazhinji, yako oda ichasvika mukati me5 - 7 mazuva ebhizinesi muUnited States uye mukati memazuva gumi ebhizinesi kune dzimwe nyika. Nekudaro, ndapota ona kuti kuendesa kunzvimbo dzekumaruwa kunogona kutora nguva yakareba.

Nguva yekutumira: Mahoridhe anowanzoitwa mukati me1 - 3 mazuva ebhizinesi. Kamwe kuraira kwako kwatumirwa, iwe uchagamuchira email yekusimbisa neruzivo rwekutsvaga.

Ruzivo rwakakosha

Kurongeka Kugadzirisa: Mushure mekunge kurongeka kwakabhadharwa, yedu yekuchengetedza inoda imwe nguva yekugadzirisa yako oda. Iwe uchagamuchira chiziviso kana yako odha yatumirwa.

Nguva dzekutumira: Muzhinji, iyo package ichaunzwa mukati menguva inofungidzirwa kusvika. Nekudaro, zuva chairo rekutumira rinogona kukanganiswa nehurongwa hwekubhururuka, mamiriro ekunze, uye zvimwe zvinhu zvekunze. Iyo nguva yekutumira yenguva ichave yakareba kupfuura yenguva dzose yemirairo inosanganisira preorder kana yakagadzirirwa zvinhu. Ndokumbira utarise kune iyo yekutsvaga ruzivo rweiyo chaiyo yekuzvitakura zuva.

Nyaya dzekutumira: Kana iwe ukaona kuti yako package haina kuendeswa mukati menguva yakatarwa; Ruzivo rwekutsvaga runoratidza kuti pasuru yakanunurwa asi hauna kuigamuchira; Kana kuti package yako inosanganisira yakarasika kana isiriyo zvinhu kana zvimwe zvinhu zveongororo, ndapota bvunza yedu yevatengi basa mukati memazuva manomwe ezuva rekubhadhara kuitira kuti titaure nyaya idzi nekukurumidza.

Kurongeka kwekutevera

Kamwe kuraira kwako kwatumirwa, iwe uchagamuchira email ine nhamba yekutevera uye chinongedzo kuti utarise kutumira kwako.

Iwe unogona zvakare kutarisa yako odha zvakananga pane yedu webhusaiti nekupinda muakaunti yako uye kutarisa yako yekuraira nhoroondo.

Kutumira Zvirambidzo

Ndokumbirawo zadza kero yemugwagwa mune ruzivo, kwete po bhokisi kana kero yemauto (apo). Zvikasadaro, isu taizofanira kushandisa EMS yekuzvitakura (inononoka kupfuura vamwe, kutora nguva 1 - 2 mwedzi kana zvakare kureba).

Mabasa ezvekutengesa uye mutero mutemo

Ndokumbira utarise kuti chero mabasa ezvetsika, mutero, kana mari yekunongedza yakavezwa panguva yekutumira ibasa remutengi. Aya anopomerwa zvinosiyana zvichienderana nenyika yekuenda uye inotemerwa nenzvimbo dzemunharaunda yemunharaunda.

Nekutenga kubva pawebhusaiti yedu, unobvuma kubhadhara chero mabasa anoshanda kana mutero unobatanidzwa neyako oda. Isu hatisi mhaka yekunonoka kunokonzerwa netsika clearance.

Package pickup mutemo

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Ongorora: Sezvo chigadzirwa chedu chinowira pasi pechikamu chakakosha, kudzoka uye kudzoserwa hazvigamuchirwe.