Nk uye til cell kuwedzera reagents (K562 Feeder Cell)
Nk uye til cell kuwedzera reagents (K562 Feeder Cell)
✔ Pre - Irradiated, Multi - Cytokine Yakavharirwa Yepamusoro NK / Til Kuwedzera.
✔ Yakaverengerwa kune ese tambo yeropa uye pbmc zvinyorwa - Mota - gadzirira.
✔ Pre - Vakakodzera kune ind Subtimissions ine yakazara chimiro data.
Overview:
The the Nk / til kuwedzera regagent ine Injiniya, Irradiated K562 Feeder Masero Kuratidza IL - 21 uye mamwe makirikina akakosha. Kana ichisanganiswa neyakajairwa immune cell media, iyi system inogonesa:
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Yakakwira - Kuwedzera Kuwedzera Kuwedzera of Nk maseru uye manhamba kubva pbmcs kana ropa reropa -
Yakakwira - Putity Varimi (> 90% target masero)
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Rapid, Sillable activation pasi pekucherechedzwa cytokine kusaina
Key application
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Activation & Kuwedzera of Nk masero, mota - NKS, uye TILS
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Kuita Kubudirira: Kutanga - Stage Optimization yeCell Serapy Inoshanda
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GMP Kugadzira: Zvinotsigira Ind - Kugonesa Zvidzidzo uye Tumira - Kubvumidza Maitiro Shanduko
Regulatory & Performance Zvakanakira
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GMP - kuenderana kuumbidzwa
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Zvisingachinjiki, yakakwira - inobereka kuwedzera (kusvika pa 1000 - peta)
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Pre - inosimbiswa kune clinical shandisa
Datasheet
NK Sero Kuwedzeredza Kubudirira uye Kupfuura Kupfuura Dzakawanda Vanopa
Anopa - -
Kusimudzira mhedzisiro yeVIRAG E E - Hancerc pane Lentivaram Campuction muNK maseru
NK CLECUCtion: VIRIC E - Hancerc Outperforms Workitors kuMoi 1 kuruboshwe Panel: Kuyerera Cytometry ye IL - 15 NK maseru neVIRIRA E - Hancerc
Panil Panel: Kuyerera Cytometry ye IL - 15 NK maseru pasina kushandurwa kweNew
Viral E - Hancerc vs. Vakwikwidzi: T - Cell Tranction paMoi 5 kuruboshwe: Kuyerera Cytomety yeVIRIRA E - Hancerc
Right: Kuyerera Cytometry yemotokari - t maseru pasina kushandurwa kweNegancer
Kusimudzira mhedzisiro yeVIRI ve e - Hancer a + virc e - Hancer d musanganiswa pane Lentiviral transduction muHSCS
Kufananidzwa kuongorora kweHSC transduction Kubudirira: Viral E - Hansera + Viral E - Hancerd Kusanganiswa Kukwikwidza Vakwikwidzi (MoI = 5)
| Siriyero nhamba | Zita reReagentand Zvinotema | Dosage (ye ~ 1 - 2e6 pbmc kutanga) |
| 1 | Serum - Yemahara Pakati | 2 l |
| 2 | IL - 2 | 4 matanda (200,000 iu / tsvimbo) |
| 3 | 0.9% NACL jekiseni | ~ 500 ml |
| 4 | 20% yevanhu Serum Albumin | ~ 15 ml |
| 5 | Ficoll Lymphocyte Acholation Solution | ~ 25 ml |
| 6 | NC - 200 kuverenga kamuri (kana kuenzana) | Sezvazvinodiwa |
| 7 | 6 - Pasina Plate | 1 |
| 8 | T25 FLASK | 1 |
| 9 | T75 FLASK | 1 |
| 10 | T75 FLASK | 2 |
| 11 | 640 cm² cell cell yebhegi | 2 |
| 12 | 15 ml Centrifigage Tube | 1 |
| 13 | 1.5 ml Centrifige Tube | Sezvazvinodiwa |
| 14 | 10 ml / 25 ml pipettes | Sezvazvinodiwa |
| 15 | 1 ml / 200 μl pipiet matipi | Sezvazvinodiwa |
Kutaurwa
| Performance paramende | Kutaurwa |
| NK / CAR - NK Kuwedzera Kupeta | > Zviuru zvishanu × (mazuva 14) |
| Cell viability | ≥90% |
| Kuchenesa Cell (CD56⁺ / CD3⁻) | > 95% |
Kuendesa ruzivo
Isu tinopa firiji kutakura pane ese mairairo. Kazhinji, yako oda ichasvika mukati me5 - 7 mazuva ebhizinesi muUnited States uye mukati memazuva gumi ebhizinesi kune dzimwe nyika. Nekudaro, ndapota ona kuti kuendesa kunzvimbo dzekumaruwa kunogona kutora nguva yakareba.
Nguva yekutumira: Mahoridhe anowanzoitwa mukati me1 - 3 mazuva ebhizinesi. Kamwe kuraira kwako kwatumirwa, iwe uchagamuchira email yekusimbisa neruzivo rwekutsvaga.
Ruzivo rwakakosha
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Nguva dzekutumira: Muzhinji, iyo package ichaunzwa mukati menguva inofungidzirwa kusvika. Nekudaro, zuva chairo rekutumira rinogona kukanganiswa nehurongwa hwekubhururuka, mamiriro ekunze, uye zvimwe zvinhu zvekunze. Iyo nguva yekutumira yenguva ichave yakareba kupfuura yenguva dzose yemirairo inosanganisira preorder kana yakagadzirirwa zvinhu. Ndokumbira utarise kune iyo yekutsvaga ruzivo rweiyo chaiyo yekuzvitakura zuva.
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Kurongeka kwekutevera
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Iwe unogona zvakare kutarisa yako odha zvakananga pane yedu webhusaiti nekupinda muakaunti yako uye kutarisa yako yekuraira nhoroondo.
Kutumira Zvirambidzo
Ndokumbirawo zadza kero yemugwagwa mune ruzivo, kwete po bhokisi kana kero yemauto (apo). Zvikasadaro, isu taizofanira kushandisa EMS yekuzvitakura (inononoka kupfuura vamwe, kutora nguva 1 - 2 mwedzi kana zvakare kureba).
Mabasa ezvekutengesa uye mutero mutemo
Ndokumbira utarise kuti chero mabasa ezvetsika, mutero, kana mari yekunongedza yakavezwa panguva yekutumira ibasa remutengi. Aya anopomerwa zvinosiyana zvichienderana nenyika yekuenda uye inotemerwa nenzvimbo dzemunharaunda yemunharaunda.
Nekutenga kubva pawebhusaiti yedu, unobvuma kubhadhara chero mabasa anoshanda kana mutero unobatanidzwa neyako oda. Isu hatisi mhaka yekunonoka kunokonzerwa netsika clearance.
Package pickup mutemo
Kamwe kuraira kwako kwasvika pane yakasarudzwa pickup point kana nzvimbo yekuzvitakura, ndapota ita chokwadi chekureva kuunganidzwa. Kana iyo package ikasatorwa mukati menguva yakatarwa, tichazotumira chiyeuchidzo kuburikidza neemail kana SMS. Nekudaro, kana iyo package ikasaunganidzwa mukati menguva yakatarwa, uye kurasikirwa kana kukuvara kunoitika semhedzisiro, mutengi achazotarisirwa. Isu tinokuyeuchidza nemufaro kuti utore yako package nekukurumidza kudzivirira chero nyaya dzinogona kuitika.
Ongorora: Sezvo chigadzirwa chedu chinowira pasi pechikamu chakakosha, kudzoka uye kudzoserwa hazvigamuchirwe.
