Kuongorora kweBenzonaseEnzymatic inoshanda
Benzonase inzira inosiyana-siyana inoita basa rinonetsa muzvikamu zveBhaibheri, kunyanya mukubviswa kwema accleic acids kubva kune biological samples. SEZVANOI NOSI - CHETE COPONCECELEACE inobvaSerratia Marcests, Benzonase zvinobudirira machira ese maviri DNA uye rna mamorekuru. Hunhu uhwu hunoita kuti zvive zvakakosha mumanyorero akasiyana-siyana, sekuchenesa mapuroteni ekudzokorora uye kuona kutendeka kwekudzivirira kwekugadzira maitiro. Vagadziri uye vanopa veBiochemical reagents vanowanzosimbisa kugona kweBenzonase mukushongedza nucids vasina kutevedzana
Michina yekuita
Benzonase zviito naHydrolyzing the phosphate yekudya zvisungo muuclec acids, kugadzira 5 'phosphate - yakamiswa oligonucleides. Iyo enzyme inobudirira zvakanyanya neyakaipesana neyako - yakamanikidzwa uye yakapetwa - yakamanikidza nucids acids, ingave mutsara, yakatenderedza, kana yakasarudzika. Uku kugona kuderedza nuclec acid kusvibiswa ndeye pivotal mumakero ipo apo nuclec acid inoshatisa inogona kukanganisa nekupeta maficha.
Zvikumbiro zveBenzonase muBiotechnology
MuBiotecchnology indasitiri, Benzonase inoshandira zvakanyanya nekuda kwekugona kwayo acid acid yekushambadzira yekukanganisa. Zvichemo zvaro zvinopinda zviyero zvakasiyana siyana, kusanganisira kugadzirwa kwevo dzakasiyana-siyana, uko kubviswa kweiyo host nucids nucids kwakakosha kuchengetedzeka uye kushanda kwechigadzirwa chekupedzisira.
Basa mukugadzirwa kwevacancine
Indasitiri yemishonga inovimba neBenzonase yehunyanzvi hwayo mukuderedza nucle acid kusvibiswa kune mapikicha ematehwe. Izvi zvinovimbisa izvo zvekudzivirira uye zvimwe protein - zvakavakirwa marpitori anochengetedza huwandu hwakanyanya hwekuchena. Vagadziri vanokoshesa benzonase mune yavo yekugadzira protocol kuti vasangane nematambudziko ekugadzirisa.
Kuvandudza kweMifananidzo yeFiltration
Benzonase haingowedzeredze nucle acid kubviswa asiwo AIDS mukuderedza vhiza yesero lysates. Kuderedzwa uku muViscosity kunosimudzira zvakanyanya kushanda kweiyo filtration uye Ultrafration maitiro, zvichitungamira kune yakagadziridzwa gohwo uye kuchena kwezvigadzirwa zvebhizimusi. Vapfigiri veBioprocessing zvishandiso zvinowanzo kurudzira Bizonase kuti vagadzirise kunatswa kunopera.
Kunzwisisa Tembiricha Yakasimba yeBenzonase Basa
Tembiricha inoita basa rakakosha mukushanda uye kugadzikana kwe enzymes, kusanganisira Benzonase. Kuziva tembiricha yakakwana yebasa rayo kunogona kukanganisa zvakanyanya kugona kwemaitiro ayo ayo anoshanda.
Enzyme Kinetics uye tembiricha
Enzyme Kinetics ane hanya nekudziya kusiyanisa, nebasa rinowanzowedzera nekuwedzera kupisa kusvika pane peak yasvika. Kupfuura ino tembiricha yekushisa, chiitiko che enzyme nekukurumidza inoderera nekuda kwekudengenyeka. Kune Benzonase, iyo tembiricha yakakwana inowanzoonekwa kunge ichitenderedza makumi matatu nematanhatu celsius, inoenderana nemamiriro emuviri.
Tembiricha - inoenderana nebasa reBenzonase
Basa renzvimbo yeBenzonase rinonyanya kukanganiswa nekudziya, inokanganisa chiyero chayo cheatari uye kugadzikana. Zvidzidzo zvinoratidza kuti pakudziya kwakadzika, chiitiko che enzyme chinoderedzwa, nepo tembiricha yakakwirira, njodzi yekudengenyeka inowedzera.
Uchapupu hwekuedza
Mune marabhoritari marongero, Benzonase inoratidza basa rakawanda kwazvo panguva dzinenge 37 degrees Celsius. Uku tembiricha inobvumira kushanda zvakanaka acid acid cleavage nepo kuchengetedza enzyme kugadzikana. Mafekitori achinyatsoziva muEnzyme Proction anowanzo calibrate maitiro avo ekuraramisa uku tembiricha, anoverengera isingadzivisiki enzyme maitiro.
Basa rekushisa mu enzyme inhibition
Nepo kudziya kwakasimba kusimudzira kwe enzyme chiitiko, ivo vanokwanisawo kupesvedzera kusanzwisisika kune inhibitors. Kuchengeta tembiricha yakakwana kunokosha mukuderedza midziyo yekudzivirira iyo inogona kukanganisa basa reBenzonase.
Inhibitor kunzwa
Kuvapo kwedzimwe reagents kunogona kudzivirira yeBenzonase chiitiko neinosvika makumi mashanu muzana pane chaiyo kutarisisa. Nekudaro, kuchengetedza iyo enzyme pakupisa kwayo kunogona kudzikisira izvi, ichiratidza kuti zvakavimbika pakuita kwekushandisa maindasitiri.
Kufunga kunoshanda kune tembiricha kudzora mumaindasitiri maitiro
Kudzora tembiricha kwakakosha kuwedzera kuwedzera kugona kweBenzonase muindasitiri - zviyero zviyero. Vaviri vagadziri uye vatengesi vanofanirwa kuita zvekushandisa mabasa avo vanoona kusingaenzaniswi tembiricha.
Tembiricha yekutarisa uye kugadzirisa
Kugara uchiongorora kwekugadzirisa tembiricha, pamwe chete ne automated tembiricha yekugadzirisa, inogona kubatsira kuchengetedza iyo enzyme chiitiko. Mafekitori anowanzoita mari mune yepamusoro tembiricha yekudzidzira matekinoroji ekuchengetedza kuti ive nechokwadi chekuti maitiro avo ekugadzira anoramba anoshanda uye mutengo.
Kuedza Kuedza kweTembiricha Optima
Kufunga tembiricha yechipfuva yeBenzonase inosanganisira kurongeka kwekuedza, kubvumira chaiyo calibration yekunyorera maindasitiri. Izvi zvinovimbisa kuti enzyme mukana wakabatanidzwa zvizere mumabasa akasiyana siyana ebietchnological maitiro.
Kuedza nzira
Standard kuedza protocol kunosanganisira kuongorora chiitiko cheBenzonase yeBenzonase kuyambuka tembiricha. Vagadziri vanowanzozvibata ongororo iyi panguva yeiyo enzyme develeme yekuvandudza mamiriro ekukurudzira ekudzidzira ekutumirwa mune dzakasiyana siyana.
Kufananidza BENZZONARE nedzimwe migumo
Benzonase anomira pakati pekupedzisira nekuda kweiyo yakafara kwazvo - Spectrum basa uye tembiricha yekushambadzira, ichiita sarudzo yakasarudzika pakati pevatengesi mhinduro dze enzyematic. Fananidzira zvidzidzo zvinobatsira kusimbisa izvi zvakanakira mukushandisa kwakasiyana siyana.
Zvakanakira pamusoro pemamwe ma enzymes
Kuenzaniswa nemamwe magumo, Benzonase inopa yakakwirira midziyo acid acid kusvibiswa kwemugwagwa wakakura wekupisa. Izvi zvinopesana zvinokatyamadza kune vagadziri vanotsvaga akavimbika uye anoshanda enzymatic mhinduro dzezvinetso zvehutachiona.
Runako Rekutsvagira mafambiro muEnzyme tembiricha Optimization
Kuenderera mberi kwekuenderera mberi kunonatsa kunzwisisa kwedu kweiyo enzyme tembiricha masimba, nechinangwa chekuvandudza kugona kweBenzonase uye kugadzikana. Tsvagurudzo iyi yakakosha kuti uchengete iyo enzyme yemucheto mumunda wemakwikwi e bioprocessing.
Inogadzirwa muEnzyme Injiniya
Kufambira mberi muProtein Injinisheni kunogona kutungamira mukuvandudzwa kweBenzonase vhiza neakavandudzwa tembiricha kugadzikana uye chiitiko. Nekutarisa pane izvi zvisina kunaka, vagadziri uye vatengesi vanogona kupa zvakanyanya zvinoshanda enzymatic mhinduro yakagadzirirwa kune chairo maindasitiri anoda.
BlueKit inopa mhinduro
Bluekit inopa mhinduro dzakarongedzwa kuti dzokorora chiitiko cheBenzonase zviitiko zvakasiyana siyana tembiricha. Masisitimu edu epurasitiki ehupamhi anove nechokwadi chekudzora tembiricha, kuwedzeredza enzyme kushanda uye kugadzikana. Nekubatanidza State - ye - the - the - Art Monitoring uye kugadzirisa matekinoroji, isu tinobatsira vagadziri uye vatengesi vanochengetedza mukana wekugadzira. Hunyanzvi hwedu muEnzymatic Solutions vanozvishongedza iwe nezvishandiso zvinodikanwa kugadzirisa matambudziko akaomarara
Kutumira nguva: 2025 - 08 - 01 13:53:28
