Ndeupi musiyano uripo pakati pe trypsin uye mamwe madzishe?

Nhanganyaya yekuRima muBiiological Systems

Paradzima, boka rakasiyana-siyana re enzymes, pivotal mune dzakawanda dzekubereka maitiro, kubva kugaya kuenda kuSero kusaina. Ivo vanoshanda nekukurukurirana nekuchena kwezvinhu zvePeptide muzvisungo zviri muPepteni, nokudaro kutamba basa rakakosha mukuchengetedza cellular homeostasis. Parthakes inodzidziswa kubva pane yavo yekuchinjisa maitiro uye substrate yakatarwa, ne serine inodzivirira kunge trypsin kuve pakati pekunyanya kudzidza. Chinyorwa ichi chinoongorora hunhu hwakasarudzika hwe trypsin mukusiyana nemamwe anodzivirira, achifunga nezvematunhu akadai semaitiro, mashandiro, uye mashandisirwo.

Trypsin: Iyo Goridhe yakaenzana muProteomics

Basa reTrypsin muMissps SpectRometry

Trypsin inotungamira iyo protoms munda nekuda kwekunyepedzera kwayo uye kushanda kwayo mukuchenesa maketani ePeptide paCarboxyl Side of lysine uye arginine zvasara. Izvi zvinonyatsoitika muzvimedu zvine mutero wakanaka kune yavo C - Terminus, inobatsira kune misa misa spectrometry (MS) kuongorora. MuPirdomics, kugona kwaTrypsin kuburitsa mapepeti ehurefu hwerefu inowedzera kuzivikanwa protein chiratidzo, chikamu chakakosha chekutsvagurudza kwesero, apo protein yakarongedzerwa tsika inodiwa.

Zvakanakira muProtein Kuzivikanwa

Proteomics zvakanyanya inovandudza trypsin yakasarudzika nzvimbo yekuzivikanwa protein. Zvidzidzo zvinoratidza kuti trypsin inogona kuziva pamusoro pe80% mapuroteni achienzaniswa nekudzima zvakaringana, zvichiita kuti zvive zvakakosha kune vanotsvaga nevatengesi mumunda. Basa rayo rinotambanudzira kubva kunonzwisisika chirwere michina kumisikidza nzira dzekurapa, dzakadai sekurapa sero.

Maitiro ehurongwa hwe trypsin

Inoshanda saiti uye CATILTICY Mechanism

Kubudirira kweChinese kweTrypsin Stemsing kubva patsime Razvo - Inotsanangurwa Nzvimbo Inoshandira, Inoratidzira Trialtic Driaad yeSerine, histidine, uye espartate. Kugadziriswa uku kunofambisa precise eptide bepa rekusungwa, zvinodiwa zvakakosha zvevatengesi vanopa enzymes dzakacheneswa dzekutsvaga uye maindasitiri ekushandisa. Kujeka kweiyo enzyme kunonzi kune kuvapo kwekuvapo kwakarongedzwa kusarudzika kusara kwespartate, iyo inokwezva yakachengetedzwa lysine uye arginine.

Isoforms of trypsin

Trypsin iripo mune akati wandei isoforms, kusanganisira cationic, anonic, uye mesotypsinogen. Imwe yega yega inobatsira mitezo yakasarudzika, ine cationic trypsin ichave yakanyanya kuwanda mumunhu pancreas yevanhu. Mesotypsin anozivikanwa kuti anokanganisika kuti aratidziridzi, achitamba chinzvimbo chakakosha mukushambadzira kweanhibitor - mapuroteni akapfuma. Kunzwisisa izvi isoforms kunokosha kuti mafekitori awirisanganiswa muEnzyme kugadzirwa kwekurapa kushandiswa.

Zvimwe zvinodhura uye zvavakajeka

Kuenzanisa neASPN uye Gluc

Aspn naGluc Target Acidic amino vakasara, vanopa data rakafanana neiyo yakapihwa ne trypsin. Iyi dziripirwo adzoreredzo dzakaenzana nePeptide mapeptide zvakakodzera kuti zviitike zvingangoongorora zvinodiwa muProtomics. Nekudaro, yavo yakakura chaizvo chaiyo inowanzoguma mukudzikiswa protein chiratidzo ichienzaniswa ne trypsin.

Chymotrypsin uye yakafara chaiyo kiroti

Chymotrypsin inosiyana ne trypsin nekuvanza hombe hydrophobic rasara, senge tryptophan uye tyrosine. Kunyange ichibata kukosha kwechikuru, kushanda kwayo kunowanzogumira kune zvimwe zvekushandisa. Broad - Rondedzero Yakanyanya Kunodzivirira seProteinase k

Sequential Diguession nzira neTrypsin

Yakavandudzwa Protein Kuzivikanwa

Kutevedzana kwekutevedzana, kunosanganisira kushandiswa kwe trypsin inoteverwa neimwe riredhi, inowedzera zvakanyanya kuratidzwa protein. Semuenzaniso, kufanotaura ne trypsin kunogona kuwedzera kuratidzwa kweProtein yeProteinase K na 731%. Zvekushandisa masero ekurapa, iyi synergy inobvumira kuti iite kuti adzivirane priving priving, yakakosha yekunzwisisa masecular mechanisms.

Dziviriro yePeptides muKugodha Denga

Zvidiki zvidiki zvinogadzirwa ne trypsin zvinodzivirirwa kubva kune kumwe kugeza muCoquential maitiro, zvichitungamira kuderera kufungidzirwa pane kufungidzira neSilico kuongorora. Iyi nzira inobatsira vanopa vanopa vanopa protomic masevhisi, sezvo inovimbisa kurongeka kwakanyanya muProtein kuzivikanwa uye chimiro.

Rondedzero inopesana neKupokana Kwakajeka Rondedzero

Dzokorora neyakanyanya kufanana, se trypsin, inofarirwa nekuda kwekukwanisa kwavo kuburitsa kufungidzira uye zvinokatyamadza zvidimbu zvePeptide. Mukupesana, yakafara chaiyo inochengetedza mhedzisiro mumasanganiswa akaoma, kutevedzera kududzirwa kwedata. Mafekitori anogadzira ma enzymes ekutsvagisa anofanira kufunga nezve misiyano iyi kuti isangane nezvinodiwa nemanenge zviitiko zvakasiyana siyana mumabasa akasiyana siyana.

Basa reZymogens muRed Reseise activation

Proterise precursor activation

Parthases inowanzo kuve yakashongedzwa seisingachinjiki zymogens kudzivirira kuparadzwa kusingadiwe kwemapuroteni. Trypsinogen, yakagadziriswa mudumbu diki, inoratidza pfungwa iyi zvakanaka. Kunzwisisa Zymogen activation ndeye mafekitori achigadzira mari yekushandisa kwekurapa, kuona kuti ma enzymes anoshanda anopiwa mumamiriro ezvinhu anodzorwa.

Mutemo na Enterrokinase

Entererokinase anotora basa rakakosha mukuita kweTripsinogen, kusimbisa kukosha kwerugwaro chairo mukudzidzira activation. Chiyero pakati peZymogen synthesis uye activation inovimbisa kuti basa polytics inoitika mukati memuviri, chinhu chinokonzeresa vatengesi vane hunyanzvi muEnzyme kuendesa maitiro ekutumira.

RODZIDZE INHITSE uye DZIDZO YEMAHARA YEMAHARA

Kudzora kweProteolytic Basa

Dzoremi inthbitor dzakakosha mukudzora proteolytic chiitiko mukati mezvinyorwa zvebhaibheri. Ivo vanodzivirira kusadzora kusingadzoreki kwemapuroteni, chiyero chakakosha mumakona epamusoro. Muchechi kurapwa, kunzwisisa zviyero izvi kwakakosha pakukudziridza kurapwa kunotadzisa basa redziviriro zvinobudirira.

ROPIZE INHITSE MUDZIDZISI

RODZIDZE INHITSE HASI KUTI VANOGONA KUTI VANZVIDZE MUZVIMBORA ZVESE ZVESE ZVESE ZVESE ZVESE ZVAKAITWA. Ivo vanopa mukana unganogona kugona, sekudzivirira kuti itsvo kukuvadzwa kubva kuna mishonga inorwisa mabhakitiriya kana kuderedza njodzi yeTumorigoSis, zvichiratidza kukosha kwavo mukutsvaga kwekurapa nekuvandudza.

Maindasitiri uye Biotechnological application ye trypsin

Basa muBiotechnology

Trypsin inoshandiswa zvakanyanya mu biotecchnological maitiro, yakadai seprotein yekugaya uye cell secsoction. Kujeka kwaro uye kushanda kwayo kunoita kuti isarudzo yavanofarira yekunyorera, kusanganisira mafekitori anogadzira zvigadzirwa zvekurapa zvekurapa.

BlueKit inopa mhinduro

BlueKIT inopa mhinduro dzakarongeka dzakagadziriswa kuti tisangane nezvinodiwa zvekutsvagisa nekunyorera maindasitiri. Nekupa yakakwira - mhando trypsin uye mamwe madzishe, bluebit inovimbisa kuti mafekitori uye mafekitori ane maturusi anodiwa kuti afambire mberi nebasa ravo muPirdomics, kurapa kwesero, uye biotechnology. Kuzvipira kwedu kumhando uye kwekutsvaga zvinzvimbo nesu semutengesi anotungamira, kukubatsira iwe kuzadzisa yakavimbika uye chaiyo kunoguma nekuedza kwako kwesainzi.