Nhanganyaya
Genomic DNASTOCKS ISHAMISANGANO YEMAHARA YEMADZIMAI MUMOLELUCARY BIOLOGY, kutamba basa rakakosha mune dzakasiyana siyana dzekutsvagisa uye zvekurapa zvinoshandiswa. Kuvandudzwa kweGenomic DNA extraction kitS yakaramba ichiita maitiro, ichiita kuti iwanikwe, inoshanda, uye yakavimbika. Ichi chinyorwa chinopera muNherain yeGenomic Skomic Skomel, Kuongorora Mhando dzakasiyana dzekuenzanisira, zviitiko, zvinetso, zvikumbiro, ongororo yemhando, automeration, kitsi, uye maitiro emangwana. Kusimbiswa kwakasimbiswa kunoiswa pane kukosha kwesero kurapa genomic genomic DNA exting kits. Kunyangwe iwe uri muongorori, chiremba, kana tekinoroji iyi, iyi yakazara yehungwaru ichapa zvakakosha kuti zviwedzere mukurumbira uye kufambira mberi muGenomic DNA Exction.
Nhungamidzo yeDNA Sking
● Ongorora yeDNA yakanatswa kits
Genomic DNA extraction kits ndeye zvakakosha zvishandiso mumanyorero ebiology laboratories. Iyi kits inorerutsa nzira yekubvisa, inovimbisa yakakwira - quality DNA inowanikwa kubva kune dzakasiyana siyana mhando. Ivo vanopa iyo yakaenzana protocol, kuderedza kusiyana uye kugadzirisa kubereka. Inowanikwa mune akawanda mafomu, aya kits inokwira kune akasiyana sampuro saizi uye yekupfuura zvinodiwa.
● Zvakakosha muzvishandiso zvakasiyana siyana
Kurudziro yeDNA yakakwenenzverwa mukutsvagurudza, Clinical diagnostics, mafambiro, uye zvidzidzo zvakatipoteredza. Mukutsvagisa, vanogonesa kudzidza kwezvinhu genetsic, genera grogent, uye Epignetic modhi. Mumakiririti ekiriniki, ivo vanoshandiswa kuongorora ma genetic disorders, kuongorora kushanda kwekurapa, uye kukudziridza mushonga wakasarudzika. Kuvimbika uye kushanda kweiyo kits iyi kuri kutarisisa kwekugadzira mhedzisiro yakarurama uye yekubereka.
Mhando dzemasampula yeDNAOLATION
● White White Blows
Genomic DNA inogona kubudiswa zvakanaka kubva kumasero machena eropa uchishandisa kits yakasarudzika. Izvi zvinonyanya kubatsira muClinical Diagnostics uye genetic kuyedzwa, uko kweropa samples inounganidzwa.
● Tsika yemasero cell
Masero emhando yeThuni anopa nharaunda inodzorwa yekufunda genetic zvinhu. Shandurwa kweDNA kubva pamasero aya akakosha pakutsvagisa muCellular uye Moleky Biology. Kits yakagadzirirwa yetishu tsika masero anovimbisa kubereka uye kuchena.
● Mhuka uye zvirimwa matishu
Kubvisa DNA kubva kumhuka uye kudyara matishu kunomisa matambudziko akasiyana nekuda kwekuvapo kweanotenderera uye akaoma cellular zvivakwa. Nyanzvi dzakasarudzika dzeDNA extura
● mbiriso uye mabhakitiriya
Mbiriso uye mabharari masero akasimba madziro emadziro ayo anoda chaiyo manzwi nzira dzekushandisa dzeDNAll in inobudirira. Kits yakagadziriswa iyi microorganisms inoshandisa enzymatic kana mechaniki yekuvhiringidza nzira kuti iburitse yakakwira - mhando genomic DNA.
Nhanho - Na - STEN STECT DNA Skfid process
● Kutanga Kuenzanisira Kugadzirira
Nhanho yekutanga muNewa yekunatswa inosanganisira kuunganidza uye kugadzirira muenzaniso. Izvi zvinogona kusanganisira centrifugation, kugeza, uye homogenizization, zvichienderana nemhando yemuenzaniso. Kugadzirira kwakakodzera kwekutanga kwakakosha kwekukudza DNA gohwo uye mhando.
● Lyns uye Inosungira Matanho
Nhanho yeLaysis inosanganisira kuputsa kuputsa masero membranes uye kuburitsa denomic dna mumhinduro. Izvi zvinowanikwa uchishandisa mafungidziro, enzymes, kana nzira dzekuchina. Chikamu chekusunga chinotevera, iko kunosarudzwa neDNA solid phange, yakadai seSilica, pasi pemamiriro mazhinji emunyu.
● Kugeza uye nhanho dzeganho
Matanho ekushambidza anobvisa zvinongedzo uye kusvibiswa, kunoita kuti kuchenyevesa kweDNA. Multiple wash buffers anoshandiswa kubvisa mapuroteni, lipids, uye mimwe madhiri marara. Pakupedzisira, nhanho yekukwevera inosunungura iyo yakacheneswa DNA kubva kuChikamu chakasimbiswa uchishandisa yakaderera munyu buffer kana mvura.
Solution - Nzira dzakavakirwa nzira dzeDNAOLATION
● Kuenzanisa nedzimwe nzira
Solution - Nzira dzakavakirwa, dzakadai se phenolm - Paunenge uchishanda, ivo vanoshanda - zvakadzama uye zvinoda makemikari ane ngozi. Mukupesana, manhamba emazuva ano extraction kits inopa yakachengeteka, nekukurumidza, uye more mushandisi - dzimwe nzira dzine hushamwari.
● Zvakanakira mhinduro - Pakavakirwa Maitiro
Kugadzirisa Izvo zvakagadzirirwa kuderedza kusvibiswa uye zvinogona kuvezwa nyore nyore kune yakakwira - Kupfuura Kubudirira. Uyezve, ivo havadi makemikari ane ngozi, achivaita kuti vadiriki vevashandi vemarabharabhari.
Zvinonetsa muDNA Sk
● Nhau dzakajairika dzakasangana
Kunatswa kweDNA kunogona kushungurudzwa, sekubereka kwakaderera, kusvibiswa, uye kusvibiswa. Inhibitors aripo mumuenzaniso anogona kukanganisa nekupaza maficha, uye kubata zvisina kunaka kunogona kuguma neDNA kutarira.
● Transgrates trips
Kugadzirisa izvi zvinonetsa, zvakakosha kutevedzera kit protocol zvine musoro. Achishandisa matsva matsva, achiramba aine nzvimbo yakachena yekushanda, uye ichine kugadzirira kwakakodzera kwekuenzanisira kunogona kudzikisira nyaya zhinji. Pamusoro pezvo, kusanganisa mhando dzekudzora kwemhando, sekuyera matanho ekutsvina uye kuchena, kunogona kubatsira kuziva uye kugadzirisa matambudziko kutanga.
Zvishandiso mukutsvaga uye mushonga
● Shandisa mukutsvaga genetic
Genomic DNA Chibvumirano ndeyekutsvagisa mu genetic kutsvagisa, kugonesa zvidzidzo paGene basa, mutemo, uye kudyidzana. Iyo inotsigira nzira dzakaita sePCR, kutevedzana, uye genotyping, iyo yakakosha yekunzwisisa genetic michina uye kukudziridza maitiro matsva ekurapa.
● Zvishandiso mumakiriniki diagnostics
MuClinical diagnostics, yakakwira - mhando genomic DNA yakakosha kune chirwere chakarurama chirwere, prognosis, uye kurapwa kwekutarisa. Zvishandiso zvinosanganisira genetic kuongororwa kusagadzikana kusagadzikana, gencer gencans, uye hutachiona hwehutachiona. Kuvimbika kweDND extraction kits kutsoropodza kupa ruzivo rwakakwana uye nenguva yekuongorora.
Mhando uye goho rekuchenesa DNA
● zvinhu zvinokanganisa kunaka
Zvinhu zvinoverengeka zvinopesvedzera kunaka uye kubereka kweDNA yakacheneswa, inosanganisira mhando yemhando, nzira yekubvisa, uye maitiro ekubata. Kuvapo kweanotaridza, kwakadai semapuroteni uye polysaccharides, kunogona kukanganisa maitiro ekuratidzira.
● Nzira dzekuyera DNA kuchena uye kusvinura
Kuongorora kuchena uye kusvinura kweDNA kwakakosha kuti uve nechokwadi chekukodzera kwayo kwekusimuka kwekuratidzira. Nzira dzakajairika dzinosanganisira spectrophotric ongororo, iyo inopa yekunyorera ratios (A260 / A280) kuti uongorore kuchena, uye fluorometric zviyero zvine tsvina uye zvakaringana uye zvakaringana.
Automation uye yakakwira yekupfuura neDNA Sk
● Tekinoroji inogonesa otomatiki maitiro
Zvekuita muDNA Skompination rave kuwedzera kukosha kwekubata hombe sampuli yekuenzanisa uye kugadzirisa basa rekushanda. Otomatiki masisitimu, akadai se robotic liquid vanobata uye otomatiki ekubvisa mapuratiforms, renji maitiro, kuderedza kukanganisa kwevanhu, uye kuwedzera kuyambira.
● Kubatsirwa kwemhinduro dzepamusoro
Yakakwira yekupfuura neDNA Skomificed Solutions yakakosha kune yakakura - Screes genetic zvidzidzo, Clinical diagnostics, uye Biebanking. Ivo vanogonesa kugadziriswa panguva imwe chete yemasampuli akawanda, kuchengetedza nguva uye zviwanikwa. Otomatiki masisitimu anopawo mhedzisiro, inosimudzira kubereka uye kuvimbika.
Kufananidza kuongororwa kwe kits
● Kuita kwekuenzanisa kwe kits yakakurumbira
Kune akawanda geniic DNA extraction kits inowanikwa kubva kune dzakasiyana-siyana Vagadziri, imwe neimwe ine zvinyorwa zvakasiyana uye maitiro ekuita. Zvinhu zvekufunga kana uchisarudza kit inosanganisira gohwo, kuchena, kusununguka kwekushandisa, uye mutengo. Fananidzira zvidzidzo uye ongororo yemushandisi inogona kupa zvakakosha ruzivo mukuita kwegits dzakasiyana.
● Ongororo yeVatengi uye Nyanzvi Pfungwa
Mhinduro yemushandisi uye nyanzvi yekuongorora kutamba basa rakakosha mukuongorora kushanda kweGenomic DNA extction kits. Kuongorora kwakanaka uye magumo kubva kune zvine mukurumbira zvinyorwa zvinogona kutungamira vaongorori mukusarudza kititi yakanakisa kune zvavanoda. Pamusoro pezvo, mhinduro kubva kune vashandisi inobatsira vagadziri kugadzirisa zvigadzirwa zvavo uye kero chero nyaya.
Ramangwana maitiro muDNA Skomelilogies
● Kubuda matekinoroji muDNA Satchection
Iyo shamba yeDNA infired iri kuramba ichisimuka, ine tekinoroji matsva anobuda kugadzirisa zvazviparadzwe. Nzvimbo dzakadai seMicrofluidics, Nuzetown Word
● Kufungidzira kune zviitiko zvenguva yemberi mumunda
Nziyo dzeramangwana muNot DNAST Iko kuvandudzwa kwemakisi epadenga kunogona kubata akasiyana siyana emhando yemhando yemhando uye mamiriro anotarisirwa kusimudzira kushanduka uye kuisazve muCNA Satchics.
Bluuit: Kupayona Dna Chekuwedzera Mhinduro
Jiangsu Hillgege, pasi pezita rechiratidzoBluekit, akazvisimbisa semutambi anotungamira mumunda weGenomic DNA yekubuda. Inowanikwa muSuzhou, ine nzvimbo dzekugadzira muShenzhen uye Shanghai, uye nzvimbo iri kuuya kuNorth Carolina - ye - the - the - art Zvigadzirwa zvebhuruu zvakagadzirirwa kusangana nezvinodiwa zvekutengesa cellular kurapwa, kutsigira kukura kwemotokari - t, tcr - t, tems cell - Nekuzvipira kuhunhu uye mhando, chikanganiso chakatsaurirwa kufambisira mberi munda wekurapa cellular kurapwa uye nekuvandudza zvibodzwa zvemurwere.
Mukupfupikisa, Genomic DNA maitiro akaomarara mumanyorero ebiology, nehurefu - zvikumbiro zvekutsvaga uye mushonga. Kufambira mberi muDNA Satchection Kits, kusanganisira izvo neBluekit, vakaita izvi kushanda uku kwakanyatsoshanda, yakavimbika, uye inowanikwa. Sezvo tekinoroji inoramba ichishanduka, ramangwana reDNA inwaxificiation inobata mikana inonakidza yekuvandudza ruzivo rwesainzi uye clinical hunyanzvi.
Kutumira Nguva: 2024 - 09 - 27 14:40:06
