Nhanganyaya
● Kukosha kweGenomic DNA Exerction
Mudenga remanyorerwo ebiology, kubviswa kweGenomic DNA yakakosha iyo inokoshesa hwaro hwezvibodzwa zvezvikumbiro, kubva pakutsvaga kuenda kuClinical Diagnostics uye Yakasarudzika Mushonga. Iyo Genomic DNA Satchection maitiro inosanganisira kuodzerwa DNA kubva kumasero yekuongorora uye kunyengedza zvinhu zvakazara, zvinopa ruzivo rwakakosha muzvirwere zvemberi, zvekushandisa biology, uye kufambira mberi kwezvinhu zvekutanga. Nekudiwa kweBurgening yeGenomic Zvidzidzo, Basa reVavengiGenomic DNA extraction kithaisati yambove yakawanda pivotal. Iyi kits restates yekubvisa maitiro, ichiverenga gohwo rakakura uye kuchena kweDNA, zvakakosha pakuratidzira mafaro.
SAMPLE MABHUKU MABHUKU
● Mhando dzemasampuli yeDNA Satchection
Kuvimbika kweDenomic DNA kune muunganidzwa weKukwikwidza - EMAIL SAMASPLES. Zviitiko zvakajairika zvinosanganisira ropa, tishu, saliva, uye buccal swabs. Imwe neimwe mhando yemhando yemhando inopa zvakanakira zvakanaka; Semuenzaniso, samples dzeropa dzakapfuma muDwo Kusarudza sampuli yakakodzera, kunyanya kana ichishandisa foni yekurapa genomic geniomic geniomic
● Zvakanakisa maitiro emuenzaniso wekubata
Kuverengera kutendeka kwemuenzaniso kunoshorwa kwebrough ew dna. Samples dzinofanira kuunganidzwa mukusvibiswa - Mahara Mahara, akachengetwa pakupisa kwakakodzera, uye akagadziriswa aine hanya kudzivirira kusvibiswa. Iyi nhanho yakakosha kuti iwe urikuita neutsinye nehunyoro)
Cell vanorera nzira
● Chemical vs. Nyora nyanzvi dzemhando dzekurara
Nhanho yeCell Lynis ndeye pivotal mukuburitsa denomic DNA kubva kuCellular Zvimiro. Chemical lysis, inosanganisira kudzora uye enzymes, zvinyoro nyoro inoputsa midziyo yemasero nemapuroteni. Nzira dzepanyama-dzakadai sekukanganiswa nemheni uye kushatirwa-inonyanya kusimba uye inogona kushandiswa kune asheri samples. Iyo sarudzo yenzira inowanzoenderana nemhando yemuenzaniso uye izvo zvakananga zvinodiwa zveGenomic DNA extction kit mukushandisa. Vagadziri magadzirirwo edunhu kuti vawedzere kunyatsoita lynsis, kuyera pakati pekusarongeka uye kushanda.
● Kukosha kweLysis muDNA Kuburitswa
Inoshamisa Lysis inovimbisa kuti zvikamu zve celluly zvakaputsika zvakakwana, uchifambisa kusunungurwa kweDNAack DNA mumhinduro. Kunyatsoitwa Lynis kuyamwisa kuurawa kweDNA uye kusvibiswa kubva purpetins uye LIPIDS, ayo anonyanya kukosha pakushandisa musero, uko kutendeka kweGenomic kuri kutendeseka.
Kubviswa kwemapuroteni uye kusachena
● Basa reMifananidzo Mukunatswa
Post - Langn, kubviswa kwemapuroteni uye kumwe kusachena kwakakosha. Sol Solvents sePhenol - Chloroforms ane tsika yakashandiswa kuDenature uye mapuroteni akapatsanurwa kubva kune nuclelic acids. Mazuva ano Genomic DNA exting Kit vanowanzo shandisa silica - based membra Iyi nhanho yakakosha pakuwana yakachena DNA yakakodzera kune chaiyo uye inotyisa nzira yekunyora.
● Centrifugation yakatsanangurwa
Centrifugation idanho rakajairika kuti iparadzanise DNA kubva kuNoturity post - Langs. Nekushandisa centrifugal simba, kurema marara marara nemapuroteni anofemerwa, nepo iyo DNA ichiramba iri munyama. Nhanho iyi inowanzokororwa mu tandem ine nzira dzekunatswa kuti dzivise kuchena uye kusunganidzwa kweiyo yakaburitswa DNA.
DNA SRAPLITAT maitiro
● Shandiswa kweIsopropanol uye ethanol
Precipation ndiyo nzira yemhando yeDNA yeDNA kubva kuSolution, inobatwa mune anenge ese dentomic DNA extction kits. Alcohols like isopropanol uye ethanol inogonesa iyo DNA kuti isarure nekuderedza iyo solubility. Kamwe chete zvakarongedzwa, mafambiro eDNA anoonekwa kana mafaira, kupa iyo yekuona yekuona yekubvisa yakabudirira.
● Kuona Filments DNA Filements
Kuvapo kweDNA DNALEMENTS kunosimbisa kunyatso svina uye kunoshanda sechiratidzo chekutanga kwekubuditsa kubudirira. Nhanho iyi, kunyange ichiita senge inosarudzika, yakakosha kuti chivimbo mune yekubvisa maitiro, ichivimbisa kuti yakakwana DNA iripo yekuongorora inotevera kuongororwa.
Kugeza precipitated dna
● Kukosha kweEthanol Kushambidza
Kushambidza blallets Nhanho iyi, kunyangwe ichiita kunge nzira, yakakosha mukudzivirira kusvibiswa izvo zvinogona kukanganisa maitiro ekutsikisa. Ethanol kugeza kwakanyatsotsanangurwa mune yega yega mushandisi bhuku neGenomic DNA yeKit vanogadzira kuti vaone kuti vashandisi vawane mukana wakakwana.
● Kuvhiringidza kuchena kweDNA
Kuchena kwakakosha seyebereko muDNA Satchection. Kusara zvinhu zvakasara kunogona kudzivirira enzymatic maitiro mukudzvinyirira mafomu ekuratidzira senge PCR uye kutsakatisa. Genomic DNA Satchection Kit Superliquirers vanosimbisa kuchena, kupa maProtocol uye zvigadzirwa izvo zvinoona kuti zvinoshatiswa zvakanyatsogezwa.
Kupera uye kugadzirira DNA
● Kusarudza iyo chaiyo buffer
Kamwe chete neDNA yakanatswa, inovharwa mune yakakodzera buffer, kazhinji te buffer kana mvura yakadzika. Sarudzo yeBuffer inogona kukanganisa kugadzikana uye kuvimbika kweDNA, Kufurira Kwakareba Genomic DNA extction kit mafekitori anowanzopa buffers akagadziridzwa kune chaiyo yekunyora.
● Kugadzirira DNA yekuedzwa
Kugadzirira kwakakodzera kweDNA kwakakosha kwekuedza kwekuedza, kunoita kuti kuwirirane nehunyanzvi hwekuongorora senge QPCr, tevere - kuyananiswa, uye kuvhara. Nzira dzakakwana dzekugadzirira dzinowedzera kutendeka kweDNA uye kuita mune izvi zvinoshandiswa.
Mhando yekudzora kweiyo inowedzera DNA
● Matekota ekuongorora Dna emhando
Mushure mekubvisa, kuongorora DNA emhando yakakosha. Spectrophotometry inzira inozivikanwa, kuyera kubviswa pa260 nm uye kupa ruzivo nezveDNA Sangano uye kuchena. Pamusoro pezvo, Gel Electrophoresis inobvumidza kuona kwekuona kweDNC Kuvimbika, Kuona chero kusvibiswa.
● Kunyevera Spectrophotometer kuverenga
Inosvitsa kuverenga kuverenga kunopa kuti inzwisisike mukusangana uye kuchena kweDNA. A 260/28/80 Ratio padyo ne1.8 inoratidza yakachena DNA, nepo kutsauka kunoratidza kusvibiswa. Kuverenga uku kunokosha mukuona kuti DNA yakwana yakakodzera yekunzwisisa maficha.
Zvinonetsa muCNA Satchection
● Zvakajairika nyaya uye kugadzirisa matambudziko
Pasinei nekufambira mberi, kusawirirana mujekishini extist vanoramba vachipfuurira, kusanganisira goho repamberi, kusvibiswa, uye kusvibiswa. Kunzwisisa izvi nyaya uye kutevera kunetsana nhungamiro - kazhinji kunopihwa nevanogadzira-kwakakosha kuti ubudirire pakurwiswa.
● Kusiyanisa nemhando dzakasiyana dzekuenzanisira
Mhando dzakasiyana dzemhando dzinoratidzira matambudziko akasiyana muzvinyorwa zveDNA Genomic DNA extction kit Suppliers dhizaini yekugadzirisa iyi misiyano, ichipa mhinduro dzakagadziriswa dzezvikamu zvakasiyana siyana zvebhasiji.
Kufambira mberi muDNA Shandurwa
● Kuvatumidzwa mune yekubvisa kits
Kuenderera mberi kufambira mberi muGenomic DNA extraction kits kwakarerutsa maitiro, kusimudzira kushanda zvakanaka, kumhanyisa, uye kuvimbika. Izvi zvigadzirwa zvinosanganisira auto, magnetic bheji tekinoroji, uye kubatanidzwa ne robotic yekunyorwa kwepamoyo, kuratidza zvinodiwa pakutsvagira kweGenomic.
● Renguva dzeramangwana muGenomic Sayenzi
Sesesainzi yeGenomic Advance, Kudiwa Kwakakwirira - Mashoko Emagedhi eDNA anokura. Nzira dzemberi dzinosanganisira zvimwe ECO - Nzira dzekutsamwa dzeCoction, kuwedzera otomatiki, uye kuwirirana kweChinyorwa
Bluekit: Piyona muGenomic uye Cellular Therapy Solutions
Jiangsu Hillgege, pasi pechiratidzoBluekit, inomirira kumberi kweiyo genomic uye cellular kurapa ivhu. Musoro wakagadziriswa muSuzhou nezvimwe zvivakwa muShenzhen uye Shanghai, uye nzvimbo iri kuuya kuNorth Carolina, Hillgee inowedzera kusvika paGlobally. Bluekit®Zvigadzirwa zvinopa mhinduro dzakarongeka dzemhando yepamusoro yekudzora muCell kurapwa, kutsigira kusimudzira kweduranced celluly maCellar ekurapa senge mota - TCR - t. Kubudikidza nemapuranga ecatic acid yekugadzira uye qc kuyedza, bhuruu ndeyekudzokorora mamiriro ekurapa kwemasero, achiunza zvigadzirwa zvekuchinja kumusika nekukurumidza uye zvakanyatsoshanda.
Post Nguva: 2024 - 12 - 05 15:07:02
