Ndezvipi zvakanakira kushandisa bca kit kit?

Nhanganyaya yeBCA PROPORINS HASTAY

Iyo BCA (BICINCHANIC ACID) protein Assay ndeye yakakosha ongororo yekuongorora mune yeiyo biochemical kutsvagisa uye cell kurapa. Iyo inofambisa iyo kuwanda kweiyo yakazara protein yekuvhara mune sampuli. Iyi nzira inoshanda pane iyo nheyo yeProtein - Copper Chapper Kuonekwa kweKuonekwa kweNzvimbo Dzakadzikira Kuzivikanwa kwaro kunobva pakushanda kwayo kwakapamhama protein akawanda.

Kuwirirana nevanogadzirisa

Kubata kuomarara samples

Chimwe chezvakanaka zvakanakira zveBCA protein dissay ndeyekuenderana nekugadzirisa. Kusiyana nedzimwe dzakawanda dzakawanda nzira dzekudzivirira, iyo BCA leartay inogona kugamuchira kusvika ku5% surfersners mukati mechikamu. Iyi ficha inonyanya kubatsira vatsvagiri uye vagadziri vachiita samples dzakaomarara, apo mafungidziro anowanzo shandiswa kugadzirisa mapuroteni. Zvekushandisa masero zvekurapa, kuenderana uku kunoonekwa kuti protein kuwanda kunoramba kuchinyatsojeka kunyangwe kuvepo kwezvinhu izvi zvinopindirana.

Kuita mumamiriro ezvinhu akaoma

Iyo BCA ASTAY inopa mhedzisiro yakavimbika kunyange mune yakaoma mamiriro ekuedza. Kubata kwaro kurwisa kusiyana-siyana mune protein kuumbwa, saAmbino acid kutevedzera misiyano nemaketani ematare, kuderedza protein. Kufanana uku kwakakosha kune vatengesi vanoda zvinoda zvinoenderana uye mukubereka mhedzisiro kuti varambe vakakwira - mhando dzemhando yepamusoro muProtein dzinogara.

Yakaderedzwa setup nguva

Kubudirira neDulition - Mahara Mahara

Sumo yeDuliation - Yemahara Mapuroteni Mitemo muBCA leday kit zvakanyanya kuderedza assay sett nguva inosvika 80%. Izvi zviyero zvakafanorongerwa zvakagadzirirwa kusanzwisisika neyelichhannel pipettes, kubvumira vaongorori uye vagadziri kuti vabvekesa maitiro pasina kurongeka. Zvekushandisa masero ekurapa, uko nguva - kuyedza kwakadzama kwakajairika, uku kugona kwakakosha.

Rakareruka Workflow

Iyi nzira yakatorwa nzira inoderedza kudiwa kwemanyorero emanyorero matanho, izvo zvisiri chete nguva - inoparadza asi zvakare inokanganisa zvikanganiso. Nekubvisa matanho aya, iyo BCA leartay inofambisa inokurumidza kutendeuka, kubvumira vatengesi kuti vasangane neakasimba kuendesa mireza uye kusimudzira chigadzirwa mumarabheratari marongero.

Yakavandudzwa Protein Youferiity

Kuyerwa kwakakwana kwemhando dzeprotein

Iyo BCA protey assay inoratidzira imwe yunifomu mhinduro kumapuroteni akasiyana zvichienzaniswa neDhayeti - Kusunga Assays seiyo Bradford Nzira. Kufanana uku kunowanikwa nekuita zvakaringana kuhuwandu hwezvisungo zvePeptide, kuderedza pfungwa dzisina kujairika kumisiyano yezvivakwa zveProtein. Kune Vagadziri vakabatanidzwa mukugadzira masero ekugadzira sero, izvi zvinovimbisa kuyerwa kwakakwana kune dzakasiyana siyana mapuroteni mafambiro, anowedzera chivimbo mumhedzisiro migumisiro.

Kuvandudzwa kushaikwa

Nekupa mhedzisiro isingadzivisike zvisinei nekuti protein kusiyana-siyana kwakasiyana-siyana kunokosha kune vanopa vanopa vanoda kugadzirira kuwanda kwekutonga kwemhando yepamusoro uye kuganhurira kwechigadzirwa. Uku kuwirirana kunoshandura kune yakawedzera data yakavimbika, yakakosha pakufambira mberi kufambisira mumusangano uye inoenderana minda.

Sensitivity uye kuongororwa huwandu

Kunzwa kwepamusoro kwekunyora kwepasi

Iyo BCA leary inopa yakakwira mwero wekunzwa, anokwanisa kuona protein kutarisisa seyakaderera se 0.5 μg / ml, uye inochengetedza mutsara kusvika ku1,5 mg / mg / mg / mg / mg / mg / mg / mg / mg / mg Iyi yakavezwa yakasimba yakasimba inoita kuti iite yakakodzera kune dzakasiyana-siyana mafaro, kubva mukutsvaga kwekutanga kuenda kufambira mberi kwekuratidzira kukura. Kunzwa kwakadaro kunokosha kana uchibata neminiti huwandu hwemapuroteni muCell kurapwa uye kutsvagurudza kwe pharmacological.

Yakakwana yekuona paramita

Iyo colorimetric yekuona pa562 nm inosimbisa mashoma chiratidzo kurasikirwa (pasi pegumi%), kubvumira vagadziri uye vatengesi kuti vabate kuwanda. Uku kurongeka kwakakosha pakukudziridza masero anoshanda masero ekurapa zvigadzirwa, uko precise protein protein yekuvharidzira inogona kubatsira zvakananga nezvekuita kwereveritic.

Kurerutsa kwekushandiswa mune ledery maitiro

Maitiro Akasarudzika

Iyo BCA inoisa maitiro akarerutswa akareruka, anobatsira kune vese vashandisi vatsva uye nyanzvi dzakanaka. Iyo yakagadzirira - kuti - shandisa reagents uye yakananga protocol kuendesa zvikanganiso, zvichiita kuti zviwanikwe kune vateereri vakawanda, kusanganisira vagadziri uye vanopa matsva kune nzira dzepuroteni.

Mushandisi - hushamwari dhizaini

Yakagadzirwa nekuzorora kwekushandisa mupfungwa, iyo BCA ASTAY kuderedza matekiniki ehunyanzvi, kufambisa ichirovedza kudzidziswa uye kukurumidza kubvumirana mukati memarabhoritari marongero. Uku kuwanikwa kunobatsira huwandu hwehuwandu hwevatengesi vanofanirwa kuchengetedza zvakakwira nekukanganisa pasina kurongeka.

Kunyatsoziva uye kuenderana

Akavimbika Mhedzisiro Kupfuura Kuedza

Iyo BCA ASTERY inopa mhedzisiro yakavimbika, inoratidzwa neyekudzikira protein - kuti - puroteni kusiyana. Uku kuenderana kwakakosha kwekuedzwa kunoda kuyerwa nekudzokorora kuyera nekufamba kwenguva, kwakadai sekurapa kwekurapa, uko kurapa kwakarongeka, uko kuwanda kwekurapa kwakarongeka kuri kwekubereka uye kusimbiswa kwemhedzisiro.

Kutungamirwa uye kuenderana

Kugadziriswa kurwisa BSA kubva kuNational Institute of Scentitute uye tekinoroji (nist) inovimbisa kuti BCA lefaay inopa yakaringana uye inobereka yakajairika curves. Iyi calibration yakakosha kune vagadziri uye vatengesi vanovavarira kuendesa yakakwira - mhando, dzakagadzirwa zvigadzirwa.

Technical zvikamu zveBCA nzira

Biochemical Mechanism

Iyo BCA nzira yakavakirwa pane kuderedzwa kwe cu²⁺kune cu⁺Nemapuroteni muAlkaline svikiro, ichiteverwa nekuumbwa kweiyo yepepuru yakaoma ne bCA, iyo inoshanda seye colorimetric chiratidzo. Izvi zviviri - STEPT Reaction inopa yakavimbika hwaro hwehuwandu hwekutanga, zvinoshanda mukutsvaga kwakasiyana-siyana kwekutsvagisa uye kusanganisa maindasitiri, kusanganisira cell kurapa zvigadzirwa.

Zvakanakira pamusoro pezvimwe zvisingaiti

Kuenzaniswa nenzira dzakaita seBradford Assay, iyo BCA nzira inopa kuwedzera kwakakura nekuda kwekuenzanisa kwaro nekukonzeresa zvinhu uye kuita kwayo kuenderana nemhando dzakasiyana dzekupururudza. Izvi zvinoita kuti isarudzo yaunosarudza yevanogadzira uye vatengesi vanoda shanduko uye yakavimbika mapuroteni ekudzivirira.

Kuenzanisa neBradford Assay

Misungo Yakakosha MuDzidziso

Nepo zvese zviri zviviri BCA uye BRADFORG LASAYS inoshandiswa zvakanyanya kune protein kuwanda, ivo vanosiyana zvakanyanya munzira yavo. Iyo Bradford Assay inovimba neDhaDha - protein inosunga, iyo inogona kuve isinganyanyi kuenderana nemhando dzakasiyana siyana mapuroteni. Mukupesana, iyo BCA ASAYICASE BAPTEY BADS - Kuonekwa Kwekuona kunopa kufanana kukuru uye kuvimbika, kunyanya kubatsira vagadziri uye vatengesi vakatarisa pane cell kurapwa uye zvakabatana zvikamu.

Zvakanakira zveBCA Gadza

Iyo BCA inosangana nayo inoenderana neyakagadziriswa, yakawedzera simba renje, uye yakareruka yebasa inopa iyo pamucheto wekushandisa zvinoda kuti aya akwanise kugona kwakananga. Kuwanirana kwayo kunoita kuti zviite kuti zviite zvakarongeka kune yakawandisa yekuedza yekuedza, chinhu chinoshora chevatengesi vanopa zvigadzirwa zvekutsvaga zvinhu zvakasiyana siyana zvekutsvagisa.

Mhedziso uye Kurudziro

Nei BCA ichisarudzwa

Iyo BCA protein inouraya yakamira kuti iwirirane neiyo nzira, yakaderedzwa setup nguva, yakawedzera protein yekufanana, uye nekurongeka. Aya hunhu anozviita akakodzera kwazvo kune zvinoshandiswa mukurapa kwesero, kupa zvakavimbika uye zvinoenderana. Kune Vagadziri uye Vanotengesa, iyo BCA leartay inopa nzira yakatenderwa kuti ive yakakwira - Huya proter protein kuwanda, yakakosha pakuchengetedza maindasitiri uye kutsigira kukura kwechigadzirwa.

BlueKit inopa mhinduro

Bluekit inoenderana nekupa mhinduro dzeBCA PROURSINAINS assay, akagadziridzwa kuti asangane nezvinodiwa zvevaongorori, vagadziri, uye vatengesi. KITS yedu yakagadzirirwa kuita kwekutarisa, kupa yakavimbika uye inowirirana mapuroteni ezvekutungamira kuyambuka huwandu hwakawanda hwekushandisa. Nebhuruu, iwe unogona kuwedzera kugona kwako kushanda uye chokwadi, kuve nechokwadi chekuti unosangana nehuwandu hwepamusoro mukurapa kwekutsvagisa uye kubereka kwechigadzirwa. Rega bhuruu rive rako rakafarirwa naye kune yako yese protein inoda.