PICHIA CORTORORIS HCP (talimalo CLASS Porotini) Retudual Revidwing Kit
PICHIA CORTORORIS HCP (talimalo CLASS Porotini) Retudual Revidwing Kit
✓ PICHIA CENTERORIS - faapitoa - leai se koluse - Faatulagaina ma Cho / HCPS
✓ Sauni - e - Faaaoga - leai se faʻataʻitaʻiga o loʻo manaʻomia
Faaaogaina Faaaoga
O lenei ki ua fuafuaina mo le aofaʻi o le faʻaaogaina o le talimalo o le Science Corpon (HCP) toe faʻatulaga i le biopharmatical oloa e maua mai PICHIA PRESTORIS O mea e nofo ai (E.G., GS115, X33), faʻaaogaina se tasi - sitepu enzyme - Livertarmem - Link Aresassors (Elisa). Mo le suʻesuʻega faʻaaoga. E le mo le poto masani.
Mataupu Faavae o le Iloaina
O le pusa e lagolagoina a Sanuisi enisa Metotia:
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Ufiufi: MicroPate lelei o le muamua - coated ma mamoe polyclonal antibody faasaga i PICHIA PRESTORIS HCPS.
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Galueaina: O faʻataʻitaʻiga / faʻataʻitaʻiga ua faʻaopopoina, sosoʻo lea ma HRP - Swewlokated mamoe posi -PICHIA PRESTORIS HCP antibodies.
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Mauaina: Ina ua uma le fufuluina, o le tmble e faʻaopopoina mo lanu lanu. O le tali ua taofi, ma ua fuaina i 450 NM.
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Fuaina: HCP Faʻatulagaina o loʻo fuafuaina mai le tulaga masani o loʻo iai (Mobilebance α HCP
Manatu Autu
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Leai se faʻataʻitaʻiga o loʻo manaʻomia (na o le le faʻamaoniaina o le faʻamaonia manaʻomia).
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Faapasi Faatulagaina: Laititi sitepu, saoasaoa faʻaiuga (<2 itula).
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Maualuga faapitoa ma le talitonuina: Sili ona lelei mo PICHIA PRESTORIS HCPS.
Faamatalaga faaleteki
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Faapitoa faataitaiga: Cell City Mamao Tuputupu Pulea, Faamamaina Bigulogcs.
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Dynamic laina: 1-100 NG / ML (masani).
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Pule Faatonutonu: E talafeagai mo le faʻagaioiga atinaʻe ma GMM o loʻo tatalaina Suʻega Suʻega.
Mafai ona faagasologa1. Fuaina
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Aumai uma vaega i le potu vevela (≥20 min).
2. Faʻataʻitaʻiga / Antinger maopoopo-
Faʻaopopo faʻataʻitaʻiga / calibrators: 100 μL / lelei
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Faʻaopopo le 1x enzyme - faʻafefeteina antibody: 100 μl / lelei (vaai le tala ①)
3. Inisiua-
Invepute i le RT, puipuia mai le malamalama, 600 rpm mo le 3 h (vaai le tala ③)
4. Fufulu-
Fufulu 1: 300 μl / lelei 1x buffer (vertical plate tulaga)
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Fufulu le 2 - 5: 300 μl / lelei 1x buffer, 5 atoa le fufuluina
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Pat mago: Ina ua uma le fufulu mulimuli, taofiofi le ipu i luga o le stosorbent pepa
5. TBM VELINUNO-
Faaopopo le TMB: 100 μL / lelei
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Atiina ae: 10 min i le RT (puipuia mai le malamalama)
6. Taofi le tali & faitau-
Faaopopo le taofi: 50 μL / lelei
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Tu i luga: 5 min
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Faitau: Od i le 450 nm (faasinomaga 620 nm)
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| Vaega | Oloa Nu | Faamatalaga | Faʻamatalaga |
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| Muai - Coated Aneti - Paichia HCP Microplate | PNA015 | 8 lelei × 12 fasi pepa | Muai - Maea ma Mamoe Polyclol Aneti -PICHIA PRESTORIS HCP antibodies. Faamaufaailogaina i le for vailaau ma le poto. Teu puipuia mai le malamalama. |
| PICHIA HCP Calibrator | Pnb015 | 2 Vials | Lyophilmized efuefu. Toefausia ma le 500 μl Calibrator Diporant (PNC002). Faʻaavanoa le 5-10 min mo le faʻateʻa (fofo e tatau ona manino). Teu puipuia mai le malamalama. |
| Calibrator dirnunt | Pnc002 | 1.5 ml × 1 tube | Manino fofo mo le toe faaleleia PIchia HCP Calibrator. |
| Asuy Dourent | Pne006 | 25 ml × 2 fagu | Mo le faʻavaivaia o calibrators, faʻataʻitaʻiga, ma le enzyme - faʻafefeteina antibody. Faʻamaonia le sili ona lelei le faʻamamaina o mea fou mo faʻataʻitaʻiga fou. |
| Fufulu paffer (10 × mafaufau) | Pnf001 | 25 ml × 2 fagu | Mo le ipu fufulu. Crystals mafai ona fausia i le maualalo vevela-faasee i le 37 ° C A leʻi faaaogaina. Faasee le 10 × ma vaipuna. |
| HRP - Faʻasalalauga Anti - Paichia HCP Antibod (100 ×) | Pnn009 | 120 μl × 1 paipa | Mamoe polyclonal antibody. Faʻamago le 100 × ma le asay diuenvant ae leʻi faʻaaogaina. Teu puipuia mai le malamalama. |
| Tmb tofa | Pnd004 | 12 ml × 1 fagu | Faʻasaina i le potu vevela (≥20 min) ae leʻi faʻaaogaina. Teu puipuia mai le malamalama. |
| Taofi le fofo | PNI002 | 6 ml × 1 fagu | Aofia ai le HCL. Fai ni mea mataga ma aloese mai le paʻu o le paʻu. |
| Faamaumau Ata | Pnk001 | 3 laupepa | Mo le ufiufi microplate fasipepa e taofi ai le filogia ma le toe faaleleia. |
Vaa Faamatalaga
Matou te ofoina atu le tausiaina o felauaiga i luga o poloaiga uma. E masani lava, o lau faʻatonuga o le a taunuʻu i totonu o le 5 - 7 aso pisinisi i le Iunaite Setete ma i totonu o le 10 aso pisinisi mo isi atunuʻu. Peitaʻi, faʻamolemole matau o loʻo lafoina i nuu i tua e ono umi se umi.
Vaaf Time: Poloaiga e masani ona gaosia i totonu 1 - 3 aso pisinisi. O le taimi lava e lafoina lau faʻatonuga, o le ae mauaina se faʻamaoniga imeli ma le sailia o faʻamatalaga.
Faamatalaga Taua
Poloaiga Faʻatau: A maeʻa le faʻatonuga, o loʻo manaʻomia e la matou fale teu oloa i se taimi e faʻatautaia ai lau faʻatonuga. O le a e mauaina se faasilasilaga pe a uma ona lafo lau poloaiga.
Faʻalauiloaina taimi: O le tele o taimi, o le afifi e tuʻuina atu i totonu o le Tala Faatatau o Taunuu. Peitaʻi, o le aso faʻaolaina moni e mafai ona aʻafia i le faʻatulagaina o le faigamalaga, o tulaga o le tau, ma isi mea i fafo. O le faʻatulagaina o taimi faʻatulagaina o le a umi atu nai lo le masani ai mo poloaiga e aofia ai le faʻatonuga poʻo mea faʻapitoa. Faʻamolemole tagaʻi i le sailia o faʻamatalaga mo le sili ona saʻo le tiliva.
Lafo mataupu: Afai e te maua o lau afifi e le i tuʻuina atu i totonu o le taimi faʻapitoa; O le sailia o faʻamatalaga e faʻaalia ai na tuʻuina atu le afifi ae e te leʻi mauaina; poʻo lau afifi e aofia ai le leiloa poʻo le saʻo aitema poʻo isi mataupu faʻapitoa, faʻamolemole faʻafesoʻotaʻi a matou tagata faʻatau auaunaga i totonu o le 7 aso o le Aso e tatau ai.
Poloaiga Ova
O le taimi lava e lafoina lau faʻatonuga, o le ae mauaina se imeli ma se numera numera ma se fesoʻotaʻiga e siaki lau uta.
E mafai foi ona e siakiina lau poloaiga i luga o la matou 'upega tafaʻilagi e ala i le ulufale i totonu o lau teugatupe ma vaʻai i lau faʻatonuga talaʻaga.
Tape tapulaa
Faʻamolemole faʻatumu i le auala auala auiliili, ae le o le PO Box poʻo le tuatusi militeli (APA). A leai, o le a tatau ona matou faʻaaogaina EMS mo le momoliina atu (e telegese nai lo isi, e fealuaʻi i le 1 - 2 masina pe sili atu).
Tiute Tiute ma Lafoga mo lafoga
Faamolemole matau o tiute o agaifanua, lafoga, po o totogi faaulufale mai, po o totogi faaulufale i le taimi o le lafoina o le matafaioi a le tagata faatau. O nei tuʻutuʻuga nei e eseese faʻalagolago i le atunuʻu nofoaga ma ua fuafuaina e le au faʻaleaganuʻu.
I le faʻatauina mai o la matou 'upega tafaʻilagi, ua e malie e totogi ni tiute talafeagai poʻo lafoga e fesoʻotaʻi ma lau poloaiga. Matou te le nafa ma le tuai ona mafua mai i le kiliaina o le Tiute.
Afifi faiga
O le taimi lava e taunuʻu ai lau faʻatonuga i le filifilia o le piki poʻo le mea e momoli ai, faʻamolemole ia mautinoa le pasiaina o le aoina. Afai o le afifi e le pikiina i totonu o le taimi atofaina, o le a matou lafoina se faʻamanatu e ala i imeli poʻo SMS. Peitaʻi, pe a le aoina le afifi i totonu o le vaitaimi faʻapitoa, ma soʻo se leiloa poʻo se faʻaletonu e tupu mai i le mea e tupu mai ai, o le tagata faʻatau o le a nafa ma le tagata faʻatau. Matou te faʻamanatu lelei atu ia te oe e aoina vave lau afifi e 'alofia ai ni faʻafitauli o ni mataupu.
Faʻamatalaga: A o le a matou oloa e paʻu i lalo o le faʻapitoa vaega, toe faʻafoʻi ma toe faʻafoʻi e le taliaina.
