Cel cytotoxicoxity metay box (adherent sini sela)
Cel cytotoxicoxity metay box (adherent sini sela)
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✔ Cfse - Faʻailogaina o le Place Spolls mo le faʻaalia manino
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✔ 7 - Aad Pisia mo le Precse Dead Deal Daing
✔Fuafuaina mo le autasi tumsor sela laina
Vaaiga lautele:
O lenei pusa ua fuafuaina e fuaina Worthorry Cell Cytotoxity faasaga i le Andhetant Profit Chells, talafeagai mo NK sela, taavale - NK sela, taavale - t sela, TCR - TLMS - ma TM sela Inor - Fasioti Tagata.
Pusa pusa:
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Cfse (Carboxyyfusfurescein Succinimidyl Ester, o le membrane - Ile Green Green Free Free Dye Dye igoa)
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7 - Aad (7 - Aʻanokangitomycin D, o le DNA - Faʻamau le DYE mo le DEG DEG DEGE)
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Feololo B (Sili ona lelei mo cytotoxictity anis)
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Messay Buffer (E mautinoa lava le mautinoa o talosaga)
Tusi autu
✔Iloiloga aoga o oloa gaosi oloa (E.g., taʻavale - t, NK sela) faasaga Faapitoa fana laina sela
✔Aiaiga Atinaʻe & Optimization i le sela togafitiga
✔Faʻatulagaina o suʻega ma le tumau suʻesuʻega mo GMP - Faʻamaonia gaosi
Tomai Faapitoa:
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Faʻavasega faʻatele (Cfse mo Sini, 7 - Aad mo Cytotoxity)
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Faapaleni protocol Mo le toe gaosia iuga
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Fetaui ma maualuga - Maua Fua Faatatau (96/384 - O pepa lelei)
Tulafono Faatonutonu & Alamanuia Faamaonia
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Faamaonia mo laina sela laina (e.g, he hoo, A549, MCF - 7)
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Talafeagai mo Ind - Maua Suesuega (ICH Q2 (R1) tausisia)
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21 cfr vaega 11- Sauniga Faʻatulagaina Faʻamatalaga Maua
NK - Taʻialaina o le CFSE + A549 sela e le tafe o le Cytometry
NK - Taʻialaina o le fasioti tagata CFSE + Ctum Beamor sela i le tafe o le cytometry
Datosheet:
| Mycoplasma afaina | Lelei / Aofai | Vavalalata vavalalata siama | Lelei / Aofai | Lactobacilills Acadiophilus | Spreaptococcus Pneumoniae | Tapunia Acetobutylicum |
|---|---|---|---|---|---|---|
| Mycoplasma osrale | 24/24 | Mycoplasm arthrdis | 24/24 | Leaga | Leaga | Leaga |
| Mycoplasma gallispicum | 23/24 | Mycoplasma Arginini | 24/24 | Leaga | Leaga | Leaga |
| Acapleplasma shorlawi | 24/24 | Mycoplasma hyorhnis | 24/24 | Leaga | Leaga | Leaga |
| Mycoplasma Fercen | 23/24 | Spiroplasma Citri | 24/24 | Leaga | Leaga | Leaga |
| Mycoplasma pneumoniae | 24/24 | Mycoplasma mimita | 24/24 | Leaga | Leaga | Leaga |
| Lisi Nu | Vaega | Faamatalaga | Teuina vevela | Pusa pito |
| HG - MD002 | Feololo B | 100 ml | 2 - 8 ° C | Pusa I |
| HG - FW001 | Messay Buffer | 100 ml | 2 - 8 ° C | - |
| Hg - cfse | Cfse | 1 Vial | - 20 ° C | TUSI II |
| HG - 7 - Aad | 7 - Aad | 1 Vial | - 20 ° C | - |
Faamatalaga
| Talosaga tapulaa | Talia tulaga |
| Sao | 70-130% toe faaleleia |
| Fufusi | 70-130% milo |
| Toe fai | Cv <10% |
| Le saʻo | Cv ≤ 10% |
Vaa Faamatalaga
Matou te ofoina atu le tausiaina o felauaiga i luga o poloaiga uma. E masani lava, o lau faʻatonuga o le a taunuʻu i totonu o le 5 - 7 aso pisinisi i le Iunaite Setete ma i totonu o le 10 aso pisinisi mo isi atunuʻu. Peitaʻi, faʻamolemole matau o loʻo lafoina i nuu i tua e ono umi se umi.
Vaaf Time: Poloaiga e masani ona gaosia i totonu 1 - 3 aso pisinisi. O le taimi lava e lafoina lau faʻatonuga, o le ae mauaina se faʻamaoniga imeli ma le sailia o faʻamatalaga.
Faamatalaga Taua
Poloaiga Faʻatau: A maeʻa le faʻatonuga, o loʻo manaʻomia e la matou fale teu oloa i se taimi e faʻatautaia ai lau faʻatonuga. O le a e mauaina se faasilasilaga pe a uma ona lafo lau poloaiga.
Faʻalauiloaina taimi: O le tele o taimi, o le afifi e tuʻuina atu i totonu o le Tala Faatatau o Taunuu. Peitaʻi, o le aso faʻaolaina moni e mafai ona aʻafia i le faʻatulagaina o le faigamalaga, o tulaga o le tau, ma isi mea i fafo. O le faʻatulagaina o taimi faʻatulagaina o le a umi atu nai lo le masani ai mo poloaiga e aofia ai le faʻatonuga poʻo mea faʻapitoa. Faʻamolemole tagaʻi i le sailia o faʻamatalaga mo le sili ona saʻo le tiliva.
Lafo mataupu: Afai e te maua o lau afifi e le i tuʻuina atu i totonu o le taimi faʻapitoa; O le sailia o faʻamatalaga e faʻaalia ai na tuʻuina atu le afifi ae e te leʻi mauaina; poʻo lau afifi e aofia ai le leiloa poʻo le saʻo aitema poʻo isi mataupu faʻapitoa, faʻamolemole faʻafesoʻotaʻi a matou tagata faʻatau auaunaga i totonu o le 7 aso o le Aso e tatau ai.
Poloaiga Ova
O le taimi lava e lafoina lau faʻatonuga, o le ae mauaina se imeli ma se numera numera ma se fesoʻotaʻiga e siaki lau uta.
E mafai foi ona e siakiina lau poloaiga i luga o la matou 'upega tafaʻilagi e ala i le ulufale i totonu o lau teugatupe ma vaʻai i lau faʻatonuga talaʻaga.
Tape tapulaa
Faʻamolemole faʻatumu i le auala auala auiliili, ae le o le PO Box poʻo le tuatusi militeli (APA). A leai, o le a tatau ona matou faʻaaogaina EMS mo le momoliina atu (e telegese nai lo isi, e fealuaʻi i le 1 - 2 masina pe sili atu).
Tiute Tiute ma Lafoga mo lafoga
Faamolemole matau o tiute o agaifanua, lafoga, po o totogi faaulufale mai, po o totogi faaulufale i le taimi o le lafoina o le matafaioi a le tagata faatau. O nei tuʻutuʻuga nei e eseese faʻalagolago i le atunuʻu nofoaga ma ua fuafuaina e le au faʻaleaganuʻu.
I le faʻatauina mai o la matou 'upega tafaʻilagi, ua e malie e totogi ni tiute talafeagai poʻo lafoga e fesoʻotaʻi ma lau poloaiga. Matou te le nafa ma le tuai ona mafua mai i le kiliaina o le Tiute.
Afifi faiga
O le taimi lava e taunuʻu ai lau faʻatonuga i le filifilia o le piki poʻo le mea e momoli ai, faʻamolemole ia mautinoa le pasiaina o le aoina. Afai o le afifi e le pikiina i totonu o le taimi atofaina, o le a matou lafoina se faʻamanatu e ala i imeli poʻo SMS. Peitaʻi, pe a le aoina le afifi i totonu o le vaitaimi faʻapitoa, ma soʻo se leiloa poʻo se faʻaletonu e tupu mai i le mea e tupu mai ai, o le tagata faʻatau o le a nafa ma le tagata faʻatau. Matou te faʻamanatu lelei atu ia te oe e aoina vave lau afifi e 'alofia ai ni faʻafitauli o ni mataupu.
Faʻamatalaga: A o le a matou oloa e paʻu i lalo o le faʻapitoa vaega, toe faʻafoʻi ma toe faʻafoʻi e le taliaina.
