Incamake yaBenzonImikorere yuburyo
Benzon ni enzyme zingana zigira uruhare runini mu rwego rw'ibinyabuzima, cyane cyane mu gukuraho acide ya nucleic ziva mubyitegererezo bya kirimbuzi. Nka NTA KUNYAZA - Endondose ikomokaSerratia Marcecens, Benzonase yizihiza neza ADN na Molekile. Ibi biranga bituma bitabanywa muburyo butandukanye, nko kweza proteine yibutsa no kwemeza ko ubusugire bwimikorere yikiruhuko. Abakora n'abaguzi bo muribinyabuzima bashimangira imikorere ya Benzon mu aciriritse acizwe acide ntakurikizwa - ibisabwa byihariye, bikaba bikaba ubushobozi bwa mugari - ubushobozi bwa spectrum.
Uburyo bwo gukora
BenzonAse Ibyakozwe na Hydrolyzing Masterters Dieters ya gieter muri acide ya nucleic, atanga pusphate 5 'fosifate - yarangiye oligonucleotide. Enzyme irakora cyane kurwanya ingaragu - yahagaze kandi ikabiri - Acide ya nucleic - yaba umurongo, uruziga, cyangwa ruguru. Ubu bushobozi bwo kugabanya umwanda wa nucleic ni ubushishozi mubikorwa aho ibisigisigi bya aciccleic bishobora kubangamira porogaramu zamanutse.
Gusaba Benzon muri Biotechnology
Mu nganda zi biotechnology, Benzonase irakoreshwa cyane kubushobozi bwa acide bwa acide. Ibisabwa byayo biranga indangarugero itandukanye, harimo umusaruro w'ikiruhuko, aho gukuraho acide yakiriye akantu kose ari ingenzi mu mutekano ndetse n'ibicuruzwa byanyuma.
Uruhare mu misaruro
Inganda za farumasi zishingiye kuri Benzon kugirango umenye ko yanduye acide ya nucleic ku rwego rwa Picogram. Ibi byemeza ko inkingo hamwe na poroteyine - Abatanga inguzanyo bakomeza urwego rwo hejuru. Abakora bashyira imbere Benzon muri protocole yabo kugirango bahurize ibipimo ngenderwaho.
Gutezimbere inzira zo kurwara
Benzonase ntabwo yongeye gukuraho acide nucleic gusa ahubwo ifasha kandi kugabanya uruzitiro rwamasaha ya selile. Uku kugabanya ubushyuhe biteza imbere ibikorwa byo kurwara neza no gukiza, biganisha ku byamuteye kunoza no kweza ibinyabuzima. Abatanga ibikoresho byibikoresho bya biogence bikunze gusaba Benzonase kugirango begereje akazi.
Gusobanukirwa ubushyuhe bwiza kubikorwa bya Benzonase
Ubushyuhe bugira uruhare runini mubikorwa no gutuza imitunganyirize, harimo Benzise. Kumenya ubushyuhe bwiza kubikorwa byayo birashobora guhindura cyane imikorere yimikorere ikora.
Enzyme kinetics nubushyuhe
Enzyme Kinetics yunvikana kugereranya ubushyuhe, nibikorwa mubisanzwe byiyongera hamwe nubushyuhe bwo kuzamuka kugeza igihe impinga igeze. Kurenga iyi bushyuhe bwiza, ibikorwa bya enzyme bigabanuka byihuse kubera gutandukana. Kuri Benzon, ubushyuhe bwiza bufatwa nkimpamyabumenyi zigera kuri 37. guhuza ibintu bya physiologique.
Ubushyuhe - ibikorwa biterwa na BenzonAse
Igikorwa cya Benzonase cyayobowe cyane nubushyuhe, bireba igipimo cya catalytic no gutuza. Ubushakashatsi bwerekana ko ku bushyuhe bwo hasi, ibikorwa bya Enzyme bigabanuka, mu gihe ku bushyuhe bwo hejuru, ibyago byo gutandukana biriyongera.
Ibimenyetso byubushakashatsi
Muri igenamiterere rya laboratoire, Benzonase yerekana ibikorwa byinshi kuri dogere 37. Ubu bushyuhe bwemerera aside iriclec exf ikora neza mugihe ukomeje gushikama enzyme. INYIGISHO ZIDASANZWE MU BIKORWA BY'UMUNTU BURUNDU ZA KANYARA MU GIKORWA BYABO GUKORA IYI CYUMWE, KOMEZA GUKORA NORNYME.
Uruhare rw'ubushyuhe muri Enzyme kubuza
Mugihe ubushyuhe bwiza bwo kuzamura ibikorwa bya enzyme, birashobora kandi kugira ingaruka kuri kongerwa kubaha. Kugumana ubushyuhe bwiza ni ingenzi muguhagarika ingaruka zibuza ibibuza bishobora kuvoma Benzonase ibikorwa bya Benzonase.
Inhibitor Sentide
Kuba hari reagents zimwe bishobora kubuza libezonase ibikorwa bigera kuri 50% kubintu byihariye. Ariko, gukomeza enzyme kubushyuhe bwagaciro burashobora kugabanya izo ngaruka, tugahore imikorere yizewe mubisabwa mu nganda.
Ibitekerezo bifatika byo kugenzura ubushyuhe mubikorwa byinganda
Igenzura ry'ubushyuhe ni ngombwa kugirango ngeneya ryoroshye rya Benzon mu nganda - Inzira. Abakora bombi nabatanga ibicuruzwa bakeneye gushyira mubikorwa sisitemu nziza zemeza imiterere yubushyuhe buhoraho.
Gukurikirana ubushyuhe no guhinduka
Gukurikirana buri gihe ubushyuhe bwo gutunganya, hamwe na sisitemu yo guhindura ubushyuhe bwikora, irashobora gufasha kubungabunga ibikorwa byiza bya enzyme. Inganda zikunze gushora imari mu bushyuhe bwo kugenzura ubushyuhe kugirango umenye neza ko inzira zabo zikomeza gukora neza kandi zigura - Ingirakamaro.
Kugena Ubushakashatsi bwa Optima
Kugena ubushyuhe bwa Opima kuri Benzon birimo ubushakashatsi bwa sisitemu, yemerera gukira neza ibyifuzo byinganda. Ibi byemeza ko ubushobozi bwa enzyme bwuzuye muburyo butandukanye bwibinyabuzima.
Kwipimisha Uburyo
Porotokone isanzwe igerageza ikubiyemo gusuzuma ibikorwa bya Benzonase hakurya yubushyuhe. Abakora mubisanzwe bakora isuzuma mugihe cyicyiciro cya enzyme kugirango menye ubushyuhe bwiza cyane bwo kohereza muburyo butandukanye.
Kugereranya Benzon hamwe nibindi bigo
Benzon ihagaze hagati yubukorikori kubera ibikorwa byayo bigari - ibikorwa bya spectrum hamwe no kwihangana kwamaturo, bikagumaho guhitamo mu gutanga ibisubizo byubwoko. Inyigisho zigereranya zifasha gushimangira izo nyungu muburyo butandukanye.
Ibyiza hejuru yizindi enzymes
Ugereranije nibindi ndogobeese, Benzonaase itanga indude yo hejuru ya acide hejuru yubushyuhe bwagutse. Ubu buryo bukomeye kubakora bashakisha ibisubizo byizewe kandi bikora neza kubibazo bigoye bya BioPusting.
Icyerekezo cyubushakashatsi buzaza muri Enzyme Hejuru Yubushyuhe
Ubushakashatsi bukomeje bukomeje kunonosora imyumvire ya enzyme ifite ubushyuhe bwa enzyme, ifite intego yo kwemeza imikorere ya Benzonase no gutuza. Ubu bushakashatsi ni ngombwa mugukomeza inkombe ya enzyme mumarushanwa yo guhatanira aoprousting.
Udushya muri Enzyme Ubwubatsi
Iterambere muri Engineering Engineerie irashobora kuganisha ku iterambere rya Benzon Disiants ifite ubushyuhe bwononone. Mu kwibanda kuri iyi nzoshya, abakora nabatanga isoko barashobora gutanga ibisubizo byuburyo bwiza bujyanye nibikenewe byinganda zikenewe.
Bluekit tanga ibisubizo
Bluekit itanga ibisobanuro bihujwe kugirango utegure libezonase kubintu bitandukanye byubushyuhe. Sisitemu yacu ya OBPECESS ihamye ireba igenzura ryubushyuhe, kuzamura Enzyme imikorere no gutuza. Muguhuza leta - ya - - Igenzura - Gukurikirana Ubuhanzi no Guhindura Ikoranabuhanga, Dufasha Abakora nabaguzi Gukomeza Umusaruro Uhoraho. Ubuhanga bwacu muri Ibisubizo Byuzuye biguha ibikoresho bikenewe kugirango ukemure ibibazo bigoye bya BioPiplex
Kohereza Igihe: 2025 - 08 - 01 13:53:28
