Ni irihe tandukaniro riri hagati ya trypsin nibindi byiza?

Intangiriro Kuri Prosease muri sisitemu y'ibinyabuzima

Protease, itsinda ritandukanye rya enzymes, ni ibintu byinshi mubinyabuzima bizima, kuva i igogora kubimenyetso byakagari. Bakora na catalizing nedsage yububiko bwa peptage muri poroteyine, bityo bagira uruhare runini mugukomeza inzu ya kagari. Protease yashyizwe mubikorwa byerekeranye nuburyo bwabo bwa kataliti hamwe nibisobanuro bya Sersite, hamwe na Serine Proteese nka Trypsin ari mubishijwe cyane. Iyi ngingo irasuzuma ibiranga bidasanzwe bya trypsate bitandukanye nibindi bimera, gusuzuma imiterere nkimiterere, imikorere, hamwe na porogaramu.

Trypsin: Ibipimo bya zahabu muri poroteomics

Uruhare rwa Trypsin muri Misa ya Spectrometry

Trypsin ayobora umurima wa poroteomics kubera umwihariko wacyo mwinshi kandi imikorere mu minyururu ya peptide ya peptide ku rubavu rwa Lysine na Argintine. Ubu buryo bwihariye buva mubice hamwe na c - termilus, nziza kuri secgetrometry nyinshi (MS). Muri porotomics, ubushobozi bwa Trypsin bwo kubyara buri gihe uburebure bwiza bworoshye kumenyekanisha proteyine, ikintu cyingenzi kubushakashatsi bwo kuvura selire, aho proteyine iranga neza.

Ibyiza mubiranga proteine

Broteomics yishingikiriza cyane kuri trypsin ibintu bidasanzwe kubiranga proteine. Ubushakashatsi bwerekana ko trypsin ishobora kumenya poroteyine zirenga 80% ugereranije na porotease hamwe nicyitegererezo cyagutse, bigatuma ntahara mubashakashatsi nabatanga isoko mumurima. Uruhare rwarwo rugurwa muburyo bwo kumva uburyo bwo guhangana no gutera imbere uburyo bwo kuvura, nkubuvuzi bwa selire.

Ibiranga imiterere ya Trypsin

Urubuga rukora hamwe na Catalytic Mechanism

Imyanya ya Catailtic ya Trypsin iva iburyo - Urubanza rukora rukora, rugaragaza urutonde rwa Serine, Histidine, no Kubogerana. Iyi miterere yorohereza ubukwe busobanutse bwa peptide, ibisabwa byingenzi kubatanga ibitekerezo byasukuye byerekana ubushakashatsi ninganda. Umwihariko wa enzyme witirirwa ukuhaba ibisigisigi bikunze kugaragara, bikurura Lysine yashizwemo Lysine na Arginine.

Isoforms ya trypsin

Trypsin ibaho muritoforms nyinshi, harimo catic, anionic, na mesotrypsinogen. Buri isoro ikora inshingano zitandukanye, hamwe na catic tysin ari mwinshi cyane muri pancreas yabantu. Mesotrypsin izwiho kurwanya abagizi babuza, bagira uruhare rudasanzwe mu kwangirika kwa Thehibitor - Poroteine ​​Zikize. Gusobanukirwa Itoma ni ngombwa kunganda zigira uruhare muri Enzyme kumuntu watangaga.

Ubundi buringanire nubusobanuro bwabo

Kugereranya na aspn na gluc

Aspn na gluc intego ya aside aside acide amino ibisigisigi, itanga amakuru yuzuzanya yatanzwe na trypsin. Izi priese zibyara ingendo zidasanzwe zibereye zibereye ibisabwa byisesengura muri poroteomics. Ariko, umwihariko wabo ukunze guturuka kuranga proteine ​​ugereranije na trypsin.

Chymotrypsin na poronomuco nini

Chymotrypsin aratandukanye na trypsin yibasiye ibisigajwe manini hydrophobike, nka tryptophan na tyrosine. Mugihe ifite imikorere ikomeye ya catalytic, imikoreshereze yayo ikunze kugarukira gusa kubisabwa. BURERA - Umwihariko urinzwe nka Proteinase k kubyara uruvange rwinshi rwa Peptates, tanga ibibazo kubisabwa na porosomics, keretse bikoreshwa hamwe na Trypsin.

Ubuhanga bukurikiranye na Trypsin

Kumenyekanisha proteine

Guverinoma ikurikirana, irimo gukoresha trypsin ikurikiwe nubundi buryange, bwongerera cyane proteine. Kurugero, kwamamazana na trypsin birashobora kongera indangamuntu ya Proteinase K na 731%. Kubisobanuro byumuvuzi bya selire, iyi snoneg yemerera kurushaho kumenyekanisha ibintu byuzuye, bingenzi kugirango dusobanukirwe inzira ya selile.

Kurinda peptride muri gereza ikurikiranye

Igihe gito cyarakozwe na Trypsin kirinzwe mu buryo bwo gukomeza gusya muburyo butandukanye, biganisha ku buryo buke kuruta guhabwa mu isesengura. Ubu buryo bufite akamaro kubatanga isoko batanga serivisi za Breeomic, nkuko bireba neza mu kumenya kwa poroteyine no kuranga.

Umwihariko utandukanye umwihatsi

Protease ufite umwihariko, kimwe na Trypsin, ntisunikwa kubera ubushobozi bwabo bwo gutanga ibice byateganijwe kandi bishobora gucungwa. Bitandukanye, umwihariko wihariye urinda ibisubizo bitunguranye, bigoye gusobanura amakuru. Inganda zitanga imishinga yubushakashatsi igomba gusuzuma itandukaniro kugirango ibone ibyifuzo byibikorwa byihariye byuburinganire muburyo butandukanye.

Uruhare rwa ZyMogene mubikorwa byo kugwiza

Kuringaniza ibikorwa bya precursor

Protease akenshi yasinzira nka ZyMogene idakora kugirango irinde kurimbuka udashaka kuringaniza kagari. Trypsinogen, ikora mumara mato, yerekana iki gitekerezo neza. Gusobanukirwa ibikorwa bya zymogen nibyingenzi mu bice bitanga uburinzi bwo gukoresha uburyo bwo kuvura, kwemeza ko imisemburo ikora itangwa muburyo bugenzurwa.

Amabwiriza na Enteronwase

Enterokinase igira uruhare rukomeye mu gukora trypsinogen, gushimangira akamaro ko amabwiriza asobanutse mu buryo butunganya. Impirimbanyi hagati ya Zymogen hamwe nikikorwa cyemeza ko ibikorwa bya promolletike bibaye mu mubiri, ikintu gifatika kubatanga impongano muri sisitemu yo gutanga Enzyme.

Kurinda ibibuza hamwe n'imikorere yabo y'ibinyabuzima

Igenzura ryibikorwa bya Proteolutic

Kurinda ibibuza ni ngombwa mugucunga ibikorwa bya porosoltike muri sisitemu yibinyabuzima. Babuza kwangirika kwa poroteyine za poroteyine, impirimbanyi ikomeye mu binyabuzima bihanitse. Mu kuvura selire, gusobanukirwa iyi mpirimbanyi ni ngombwa mugutezimbere imiti ikoreshwa ibikoresho byoroga ibikorwa byiza.

Kurinda ibibuza muri therapeutics

Kwirinda ababuza kutagira uruhare gusa mumabwiriza ya physiologique gusa ahubwo no mubikorwa byatangaga. Batanga inyungu zishobora kuba zishobora kwangiza impyisi muri antibiotique cyangwa kugabanya ibyago, byerekana akamaro kabo mubushakashatsi bwubuvuzi niterambere.

Gusaba inganda na biotechnologique ya trypsin

Uruhare muri Biotechnology

Trypsin ikoreshwa cyane muburyo bwibinyabuzima, nka poropaine igogora hamwe no kwanga selile. Umwihariko wacyo no gukora neza bituma habaho guhitamo porogaramu nyinshi, harimo mu nganda zitanga ibicuruzwa byo kuvura akantu, aho ibisobanuro mubicuruzwa byimikorere ya selile, aho ibisobanuro mubikorwa byimiterere ni ngombwa.

Bluekit tanga ibisubizo

Bluekit itanga ibisubizo byuzuye bihurira kugirango byuzuza ibisabwa byubushakashatsi ninganda. Mugutanga hejuru - Ubwiza Trypsin nibindi byondaga, Bludikit byemeza ko laboratoire nimpande zigira ibikoresho bikenewe kugirango bateze imbere akazi kabo muri poroteomics, kuvura selire, na biotechology. Ubwitange bwacu bwo gutondekanya ubuziranenge no guhanga udushya dushyirwaho nkuwatanze isoko, kugufasha kugera kubisubizo byizewe kandi byuzuye mubikorwa byawe bya siyansi.