IRIBURIRO RY'IBISANZWE DNA
Kwipimisha bya ADN bivuga uburyo bwo gusesengura bukoreshwa mukumenya no kugereranya ibimenyetso byinshi bya ADN biguma mubicuruzwa bibinyabihonyo nyuma yo gukora ibikorwa. Ubu bwoko bwo kwipimisha ni ngombwa kugirango tumenye umutekano kandi ubuziranenge bwa biologique, harimo no kuvura selile, inkingo, hamwe na antibodic. Kubaho kwa ADN ibisiba bisigaye, cyane cyane ADN bikomoka kuri selile yakira nka E.COLI, isaba ingaruka zirimo imbaraga zirimo imbunonity nigirirwa. Kubwibyo, ibizamini bisigaye bya ADN nigice cyingenzi cyo kugenzura ubuziranenge mumikorere yibinyabuzima.
Ibisobanuro n'akamaro
Ikizamini gisigaye cya ADN kirimo gutahura no kumenyekanisha ibice bya ADN bisigaye bivuye muri selile zakorewe mugihe cyo gukora ibinyabuzima. Ibi bice birashobora gutandukana mubunini nubwinshi, kandi niminota ntarengwa birashobora kuba ngombwa. Akamaro k'ibizamini bya ADN bidashoboka ntibishobora gukabya - byerekana ko ibinyabuzima bitera imbere bihura n'ibipimo ngenderwaho byo kugenzura umutekano no gukora neza, bityo birinda ubuzima bwo kwihangana.
SHAKA KUGENZURA UBUNTU
Igenzura ryiza mumisaruro y'ibinyabuzima bikubiyemo ibyiciro byinshi, uhereye kubikoresho bibisi kugirango usuzume ibicuruzwa byanyuma. Kwipimisha ADN nintambwe ikomeye muri uru rwego. Ryemeza ko gahunda yo kwezwa yakuyeho ibintu bidakenewe bidakenewe, kwemeza ibicuruzwa byanyuma byubahiriza amabwiriza yumutekano byashyizweho nabayobozi bashinzwe kugenzura nka FDA na EMA.
Uruhare rwa ADN isigaranye muri Biyoni
● Ubwoko bwa Biopharmaceticals
Ibinyabuzima bikubiyemo ibicuruzwa byinshi, birimo antibodies monoclonal, inkingi za poroteyine, inkingo, hamwe no kuvura akabari. Buri cyiciro gifite inzira zidasanzwe, nyamara byose birashobora kwibasirwa no kwanduza ADN.
INGINGO Z'IMITERERE YA HNA
ADN isigaye ikomoka cyane cyane muri selile zakiriye ikoreshwa mubikorwa. Ingirabuzimafatizo zisanzwe zirimo selile za bagiteri nka E.COLI, selile zisebanya, selile zamabara, na selile udukoko. Mugihe cyo gukora ibinyabuzima, ingirabuzimafatizo zinyeganyezwa kugirango zisarure ibicuruzwa byifuzwa, birashoboka ko birekura ibikoresho byabo bya geneti muvange.
Amahame ya Taqman Probe muri ADN yatahuye
● Uburyo bw'ibikorwa
Ikipe ya Taqman - Assied Assay nubuhanga bukoreshwa cyane kubitubanza bya ADN bisigaye. Ubu buryo bukoresha ibimenyetso byanditseho byerekana ko hakurya ya ADN yihariye ikurikirana. TAQ Polymeyese Enzyme noneho isukuraho ingendo mugihe cya PCR Yongerewe, utandukane irangi rya fluorescent kuva kumwanga no kubyara ikimenyetso giterewe.
● Ibyiza bya Taqman Probe
Imwe mu nyungu z'ibanze za Taqman Probe ni umwihariko. Ubushobozi bwibikorwa bwo kuvanga urukurikirane rwihariye buremeza ko intego ya ADN yonyine yongerewe kandi imenyekana, igabanya ibyiza. Ubu buryo butanga kandi kumva cyane, bigatuma ari byiza gutahura urwego ruto rwa ADN.
E.COLI nka selile yo kwakira ibinyabuzima
● Kuki E.COLI ikunze gukoreshwa
E.COLI ni selile yakiriye yatoranijwe muri biotechnologiya kubera ubwiyongere bwayo bwihuse, neza - byaranze genetiki, nubushobozi bwo kwerekana urwego rwo hejuru rwibitabo byandika. Iyi mico ikora e.coli igiciro - Guhitamo neza kandi neza kuri binini - Umusaruro wa rugezi.
● Ingaruka zo gusiba e.coli ADN
Nubwo ibyiza byayo, ikoreshwa rya E.COLI rizana ibyago byo kwanduza ADN. Iyubahwa gisigaye irashobora gutera ibibazo byumutekano, nkibishoboka byo kwimura leta ya horizontal cyangwa kuboneka. Kubwibyo, uburyo bukabije bwa ADN bwa ADN nibyingenzi mugihe ukoresheje E.COLI nka Nyiricyubahiro.
Uburyo bwo kumenya
● Tekinoroji ikoreshwa mubyinshi
Ubuhanga bwinshi bukoreshwa mukumenya cyane ADN usigaye, harimo na QPCR, Digital PCR, naho ibisekuru - Igisekuru - Igisekuru. Buri buryo butanga inyungu zitandukanye mubijyanye no kumva, umwihariko, kandi winjijwe.
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Mu kwipimisha ibisigazwa bya ADN, kumva neza kandi ukuri biratangaje. Tekinike nka QPCR na Digital PCR irashobora kumenya ADN kurwego rwa FEMOGM, itanga sentutivite irenze kugirango umutekano wibicuruzwa. Icy'ingenzi ni ngombwa, kuko cyemeza ko hagira ibyiringiro by'ibizamini, bigatuma icyemezo cyizewe - gufata neza.
Akamaro ko Gutahura urwego rwa FG
● Ibisobanuro byurwego rwa FG
Urwego rwa FG bivuga femigractomes, igice cyo gupima gihagarariye 10 ^ - garama 15. Kumenya ADN kurwego rwa FeMogram byerekana ko bagabana cyane bashoboye kumenya ibimenyetso byibisobanuro bya geneti.
Shunge
Ubushishozi buke mu kwipimisha bwa ADN nigihe gikomeye cyo kubungabunga umutekano wibinyabuzima bya Biopharmacetical. Kumenya ADN kurwego rwa FG cyemerera kumenyekanisha ndetse nabanduye bato, kureba ko ibicuruzwa byanyuma ari byiza cyane bishoboka kandi bihura nubuziranenge rusange.
Ingamba zo kugenzura ubuziranenge kumusaruro wibinyabuzima
Gukenera kwipimisha ADN
Gukenera kwipimisha ADN muri biopharmacedical ituruka ku ngaruka zishobora guteranwa no kwanduza kamera. Ibigo bishinzwe kugenzura byanze imipaka ku rwego isigaye ya ADN, bisaba uburyo bukomeye bwo gupima imbaraga kugirango tumenye.
Ibipimo ngenderwaho
Ibipimo ngenderwaho kubisigi bitandukanijwe bitewe nubwoko bwa bioharmacetical. Kurugero, FDA na EMA byashyizeho umurongo ngenderwaho ryerekana imipaka yemewe kuri ADN ibisizwe mubicuruzwa bitandukanye. Gukurikiza aya mahame ni ngombwa kugirango ubone ibicuruzwa no kurekurwa isoko.
Porogaramu mu musaruro wa poroteyine
● Urubanza rwihariye
Mubyatangajwe na poroteyine, ibizamini bya ADN byahumetswe ni ngombwa kugirango ubone ubuziranenge. Inyigisho zihariye zigaragaza ikoreshwa ryaE.COLI ADNET KAIDs Gukurikirana no kugenzura urwego rwa ADN rwanduza, rushinzwe kubahiriza ibipimo ngenderwaho.
● Ibyiringiro bifite ireme
Ubwishingizi bwiza mumusaruro wa porotemune birimo ibice byinshi byo kwipimisha no kwemeza. Ikizamini cya ADN gisigaye kigira uruhare runini muriki gikorwa, gitanga amakuru akenewe kugirango yemeze ko gahunda yo kwezwa yakuyeho ingaruka neza.
INGORANE MU BIKORWA BYA ADN
Ingorane za tekiniki
Imwe mu mbogamizi z'ibanze mu kwipimisha ADN nuburyo bwa tekiniki yo kumenya no gushyiraho gahunda yo hasi ya ADN. Ibintu nkibi byintangarugero matrix, guhagarika ADN, no kwitoza kubuza birashobora kugora inzira yo kugerageza.
● Gutsinda inzitizi zisanzwe
Gutsinda inzitizi bisaba gukoresha tekinike yateye imbere na protocole nziza. Abakora n'abaguzi ba E.COLI DNA ibisigazwa bisigaye bikomeza gukora kugirango batunganize ibicuruzwa byabo, batezimbere kumva neza kandi byihariye kugirango bahuze inganda.
Ibihe by'ejo hazaza mu kwipimisha ADN
Gutera imbere mu ikoranabuhanga
Iterambere ryikoranabuhanga ryiteguye guhinduranya ibizamini bya ADN. Udushya nka CrispR - Assay ashingiye kuri PCR, hanyuma ahakurikira - Igisekuru - Igisekuru - Igisekuru gikurikirana bitanga inzira nshya zoroshye cyane kandi zidasanzwe za ADN.
● tekinike igaragara nibikoresho
Ubuhanga bugaragara nibikoresho mubyiciro bya ADN bya ADN byasezeranijwe kugirango kunonosora neza kandi neza ingamba zo kugenzura ubuziranenge. Iterambere rizafasha abakora imiti yo guhuza ibipimo ngengamikorere bigenda byiyongera mugihe hazabungabunga umutekano wibicuruzwa.
Umwanzuro
Ibizamini bya ADN nibice bikomeye byo kugenzura ubuziranenge kumusaruro wibinyabuzima. Gutahura no kumenyekanisha ibimenyetso bya ADN ni ngombwa mu kubungabunga umutekano no gukora neza bya biologique. Ubuhanga bwateye imbere nka Taqman Probe itanga sennititivite kandi yihariye kugirango yuzuze ibipimo ngenderwaho. Mugihe inganda zikomeje guhinduka, udushya mubizamini bya ADN bizagira uruhare runini mugukomeza urwego rwo hejuru rwimiterere yimiterere yibicuruzwa.
● IbyerekeyeBluekit
Jiangsu Hillgene, munsi yindirimbo Bluekit, yashyizeho icyicaro cyayo muri Suzhou hamwe n'ibiti 10,000㎡ gmp na R & D Centre. Hamwe n'imbuga zo gukora muri Shenzhen, Shanghai, n'urubuga rushya rurimo kubakwa muri Carolina y'Amajyaruguru, Hillgene arigura ko isi igenda. Isosiyete irongore mu iterambere ry'imiterere myiza y'ibicuruzwa bishinzwe kuvugurura ibikomoka ku buvuzi bwa selire, ishyigikira abafatanyabikorwa mu iterambere ryiza - t, TCR - T, na selile ya Stem - Ibicuruzwa bishingiye ku giti cye - Ibicuruzwa bishingiye ku giti - Ibicuruzwa bishingiye ku giti cye. Bluekit yiyemeje gutera imbere udushya tw'ingabo no gutanga ibisubizo byerekanwe kubicuruzwa bya selile.
Igihe cyagenwe: 2024 - 09 - 23:17:04
