Intangiriro
Gukuramo ADN ya amna nuburyo bufatika muri biologiya ya molekile, bagira uruhare rukomeye muburyo butandukanye bwubushakashatsi nubuvuzi. Iterambere ryaADN ya ADN ya AmnaS yahinduye iyi nzira, bigatuma ishobora kugerwaho, ikora neza, kandi yizewe. Iyi ngingo ihitana mubintu bya ADN ya Amoko, Gushakisha Ubwoko bwicyitegererezo, Gusukura, Ibibazo, Gusuzuma neza, Gusuzuma neza, Kwikora, Ibikoresho by'ubucuruzi. Hashingiwe ku gaciro kanini ku kamaro k'ibikoresho bya selire ya sernic dN. Waba umushakashatsi, umutekinisiye, umutekinisiye wa laboratoire, ubwo buyobozi bwuzuye buzatanga ubushishozi bwingenzi mubibazo no gutera imbere muri demomic DRN.
Intangiriro Kuri Ibikoresho bya ADN
Incamake y'ibikoresho bya ADN
Abanyamenna ba dna ya arna nibikoresho byingenzi muri laboratoire za molekile. Ibi bikoresho byoroshya inzira yo gukuramo, kubuza - ADN ifite ubuziranenge iboneka muburyo butandukanye bwintangarugero. Batanga protocole isanzwe, kugabanya impinduka no kunoza indwara. Kuboneka muburyo bwinshi, iyi mikiniki yinjira muburyo butandukanye hamwe nibisabwa.
● Akamaro muri porogaramu zitandukanye
Ibikoresho bya ADN biratangazwa ningirakamaro mubushakashatsi bwa genetike, gupima ubuvuzi, abacamanza, nibidukikije. Mu bushakashatsi, bashoboza kwiga ibikoresho bya genetike, gene imvugo, hamwe no guhindura epigerestic. Mu igenamiterere ry'amavuriro, zikoreshwa mu gusuzuma indwara za genetique, gukurikirana imiti ivurwa, no guteza imbere imiti yihariye. Kwizerwa no gukora neza kuri ibi bikoresho birakomeye kugirango bishoboke gutanga ibisubizo nyabyo kandi byororoka.
Ubwoko bw'icyitegererezo cyo kwigunga kwa ADN
VeriViri yamaraso yera
ADN ya Nenomic irashobora gukurwa neza na selile yera ukoresheje ibikoresho byihariye. Ibi ni ingirakamaro cyane mubuvuzi no kugerageza genetike, aho imitwe yamaraso irasakuza.
● Ingirabuzimafatizo z'umuti
Ingirabuzimafatizo z'umuco za tissue itanga ibidukikije bigenzurwa no kwiga ibikoresho bya sertike. Gukuramo ADN uhereye kuri selile ningirakamaro kubushakashatsi muri biologiya na molecular. Ibikoresho byagenewe selile yumuco tissue komeza umusaruro mwinshi kandi ufite ubuziranenge.
● Imyenda y'inyamaswa n'ibimera
Gukuramo ADN uhereye mu ingirangingo n'impanuka bitera ibibazo bidasanzwe bitewe no kuba abagizi ba nabi n'inzego zigendanwa. Ibikoresho byihariye bya DNA byateguwe kugirango bikemure ibi bintu bigoye, kwemeza lysis nogusukura neza.
● Umusemburo na bagiteri
Umusemburo n'umusego ba bagiteri ufite inkuta zakagari zisaba uburyo bwihariye bwo gukuramo ADN. Ibikoresho bidoda kuri mikorobe Koresha uburyo bwo guhungabanya agaciro cyangwa imashini kugirango irekure isuku - nziza ya amoko.
Intambwe - na - Intambwe ya ADN
Imyiteguro ya mbere
Intambwe yambere muri ADN ikubiyemo gukusanya no gutegura icyitegererezo. Ibi birashobora kubamo centrifugation, gukaraba, no guhugura, bitewe n'ubwoko bw'icyitegererezo. Icyitegererezo gikwiye ni ngombwa kugirango ubone umusaruro wa ADN n'ubwiza.
● LYSIS no guhuza ibyiciro
Intandaro zirimo kumenagura inkuta za selire no kurekura ADN ya Newnation mubisubizo. Ibi bigerwaho hifashishijwe ibikoresho, enzymes, cyangwa uburyo bwubukanishi. Icyiciro cyo guhuza ibikurikira, aho ADN ihitamo icyiciro gikomeye, nka Silica, mu mikino miremire.
Gukaraba n'intambwe zo hejuru
Gukaraba Intambwe Kuraho Kuraho umwanda n'ibyandumiwe, kureba niba isuku ya ADN. Amashanyarazi menshi akoreshwa mu gukuraho poroteyine, lipide, nundi myanda ya selile. Hanyuma, Intambwe yo hejuru irekura ADN isukuye mu cyiciro gikomeye ukoresheje umunyu muto cyangwa amazi.
Igisubizo - Bishingiye kuri ADN
Kugereranya ubundi buryo
Igisubizo - Uburyo bushingiye, nka Fhenol - Gukuramo chloroform no gusohoka, ni tekiniki gakondo yo kwezwa kuri ADN. Mugihe akamaro, ni umurimo - ubushishozi kandi bisaba imiti ishobora guteza akaga. Ibinyuranye, ibikoresho bya ADN bigezweho bya ABN bitanga umutekano, byihuse, ndetse nabakoresha benshi - ubundi buryo bwa gicuti.
● Ibyiza byigisubizo - Bishingiye
Igisubizo - KNE zishingiye kuri ADN zitanga ibyiza byinshi, harimo umusaruro wa ADN High, ubuziranenge, no kubyara. Byaremewe kugabanya umwanda kandi birashobora guhindurwa byoroshye - progaramu ikoreshwa. Byongeye kandi, ntibikenera imiti ishobora guteza akaga, bigatuma bahuza abakozi ba laboratoire.
INGORANE MU GUyehora ADN
Ibibazo Bisanzwe byahuye nabyo
Gusuga kwa ADN birashobora gucibwa ibibazo, nkumusaruro muto, kwanduza, no gutesha agaciro. Abayabujije muri sample barashobora kubangamira porogaramu zamanutse, kandi uburyo budakwiye burashobora kuvamo kogosha ADN.
Inama zo gukemura ibibazo
Kugira ngo ukemure ibyo bibazo, ni ngombwa gukurikiza protocole ya Kit Protocole. Ukoresheje reagene nshya, kubungabunga ibidukikije bisukuye, kandi ushishikarize ibyitegererezo byingenzi birashobora kugabanya ibibazo byinshi. Byongeye kandi, gushiramo intambwe yo kugenzura ubuziranenge, nko gupima ADNA kandi ubuziranenge, birashobora gufasha kumenya no gukemura ibibazo hakiri kare.
Gusaba mubushakashatsi nubuvuzi
Gukoresha mu bushakashatsi bwa genetike
Gukuramo ADN ya amna ntibisanzwe mubushakashatsi bwa genetike, bushoboza amasomo kubikorwa bya gene, amabwiriza, hamwe n'imikoranire. Ishyigikira tekinike nka PCR, ikurikirana, hamwe na genootyping, nibikoresho byingenzi byo gusobanukirwa uburyo bwa geneti hamwe no guteza imbere ingamba nshya zumutera.
● Gusaba mu gusuzuma kwa clinical
Mu kwisuzumisha amavuriro, hejuru - nziza cyane ADN ya Nenamic ni ngombwa mu gusuzuma indwara yuzuye, prognosis, no gukurikirana ivuriro. Porogaramu irimo kugerageza genetike kubibazo byarazwe, kanseri ya kanseri, no gutahura indwara zanduza. Kwizerwa kw'ibikoresho byo gukuramo bya ADN birakomeye mu gutanga amakuru asobanutse kandi ku gihe.
Ubuziranenge n'umusaruro wo kweza ADN
● Ibintu bireba ubuziranenge bwa ADN
Ibintu byinshi bigira ingaruka nziza kandi bitanga umusaruro wa ADN usukuye, harimo ubwoko bwicyitegererezo, uburyo bwo gukuramo, nuburyo bwo gukemura. Kubaho kw'abanyabugizi baho, nka protewuna na Polsaketi, birashobora kugira ingaruka kubisabwa kumanuka.
● Uburyo bwo gupima ADNET NA CERNTRATION
Gusuzuma ubuziranenge no kwibanda kuri ADN ni ngombwa kugirango tubone ibyo bukwiye kuri porogaramu za Downstzo. Uburyo busanzwe burimo gusesengura ibintu, bitanga ibipimo byinjira (A260 / A280) gusuzuma ubuziranenge, no gupima fluorometric, bitanga ibipimo byibandaho kandi bitanga ibintu byoroshye kandi byumvikana.
Automation na Hejuru Winjiza ADNA ADN
● Technologies ifasha inzira zifasha
Automation muri ADN yo kweza yarushijeho kuba ingenzi mugukemura byinshi byingumi no kuzamura imikorere yakazi. Sisitemu yikora, nka robos za robos zisumba rya robo hamwe nibibuga byo gukuramo byikora, kuzamura inzira, kugabanya amakosa yabantu, no kwiyongera.
Inyungu zo hejuru
Ibisubizo byinshi bya ADN byatangajwe ni ngombwa kuri binini - Igipimo cyimiti yipimisha, Gupima ubuvuzi, na Biobanking. Bashoboje gutunganya icyarimwe gusangira byinshi, gukiza igihe n'umutungo. Sisitemu yikora nayo itanga ibisubizo bihamye, bikongeza ingorane no kwiringirwa.
Isesengura ryibikoresho byubucuruzi
Kugereranya imikorere ya kice zizwi
Hano haribintu byinshi bya ADN bya ADN biboneka kubakora bitandukanye, buri kimwe gifite ibintu bitandukanye nibiranga imikorere. Ibintu ugomba gusuzuma mugihe uhitamo ibikoresho birimo umusaruro, ubuziranenge, byoroga gukoreshwa, nibiciro. Inyigisho zigereranya hamwe nisubiramo ryabakoresha birashobora gutanga ubushishozi bwingenzi mubikorwa byibikoresho bitandukanye.
● Isubiramo ry'abaguzi n'ibitekerezo by'inzobere
Ibitekerezo byabakoresha hamwe nimpuguke zishingiye ku ruhare rukomeye mugusuzuma imikorere yikikoresho cya Amna cya DNA. Isubiramo ryiza nibikorwa bivuye mu masoko bizwi birashobora kuyobora abashakashatsi muguhitamo ibikoresho byiza kubyo bakeneye. Byongeye kandi, ibitekerezo byabakoresha bifasha abakora neza ibicuruzwa byabo no gukemura ibibazo byose.
Ibihe bizaza muri Technologie ya ADN
● Ikoranabuhanga rigaragara mu gukuramo ADN
Umwanya wa ADN ukomeza guhinduka, hamwe nikoranabuhanga rishya rigaragara kugirango dukemure imipaka. Udushya nka microfluidics, Nanotechnology, na magnetique isadi ya magnetiki - Gukuramo bishingiye ku buryo bwo gukora uburyo bunoze kandi busobanutse bwa ADN.
Guhanura Ibihe bizaza mu murima
Ibihe by'ejo hazaza mu cyezwa cya ADN birashoboka ko twibanda ku buryo bwo kwibanda, miniturution, no kwishyira hamwe na tekiniki za molecular. Iterambere ryibikoresho byisi yose bishobora gukemura ubwoko butandukanye bwurugero hamwe nibihe biteganijwe ko bizamura impinduka no guhinduranya muri ADN yakuramo akazi.
Bluekit: Ubupayiniya ADN Gukuramo Ibisubizo
Jiagsu Hillgene, munsi yizinaBluekit, yishyizeho nk'umukinnyi ukomeye mu murima wa ADN ya Anamic. Iherereye muri Suzhou, hamwe n'imbuga zo gukora muri Shenzhen na Shanghai, n'urubanza ruzaza muri Carolina y'Amajyaruguru, Hillgene itanga Leta - ya - Ibicuruzwa byo gukuramo Art. Ibicuruzwa by'Ubururu byateguwe kugira ngo byujuje ibyangombwa bifatika byerekana imiti ya selile, gushyigikira iterambere ry'imodoka - t, TCR - T, na selile ya Stem - Ibicuruzwa bishingiye. Hamwe no kwiyemeza guhanga udushya no gutangaza, Hillgene yeguriwe gutera imbere umurima wubuvuzi bwa selire no kuzamura umusaruro wihanga.
Muri make, kweza ADN ya Anamic ni inzira ikomeye muri biologiya ya molecular, hamwe nagutse - ikoreshwa rya porogaramu mubushakashatsi nubuvuzi. Iterambere mu bikoresho bya ADN, harimo n'abarya blubkit, byatumye iyi nzira ikora neza, yizewe, kandi irashobora kuboneka. Mugihe ikoranabuhanga rikomeje guhinduka, ejo hazaza h'amayeri ya ADN ifite ibishoboka byo kuzamura ubumenyi bwa siyansi no guhanga udushya.
Igihe cyagenwe: 2024 - 09 - 27 14:40:06
