Ni ibihe bice bishyirwa mu gikoresho cya BSA?

Intangiriro KuriBSAs

Bovine Sertum Albumain (BSA) ibikoresho nibikoresho byingenzi mubinyabuzima bya laboratoire. Ibi bikoresho byateguwe kugirango bifashe abashakashatsi nabatekinisiye ba laboratoire mugukora porogaramu zitandukanye, harimo umubare wa poroteyine, Enzyme - Ihujwe Imyumunitso isaba (Elisa), nibindi byinshi. Gusobanukirwa ibice bishyizwe mubikoresho bya BSA ni ngombwa kugirango ukore ubushakashatsi neza kandi tugere kubisubizo nyabyo.

Uruhare rwa BSA mubushakashatsi

Akamaro ka BSA

BSA ikoreshwa cyane nkibisanzwe muri poroteineti. Ikora nkumukozi uhamye kuburyarya kandi nkigishushanyo mbonera cya poroteyine. Guhinduranya kwayo bituma bihurira nibice bikunze kugaragara mubyiciro no gupima.

BSA muri proteine ​​ifata

Muri poroteyine ifatanije, BSA ikunze gukoreshwa nkicyeshya zerekana kwibanda kuri poroteyine itazwi. Ifasha muri kalibration, kureba niba ubushakashatsi butanga ibisubizo byizewe kandi byororoka.

Ibice bya bsa ibikoresho bya BSA

Ibice by'ibanze

• BSA ubuzirane:Ibice bikuru byimikino, bikoreshwa mugukora umurongo usanzwe.
• Ibisubizo bya Buffer:Ibyingenzi mugukomeza PH na Style yingengo yicyitegererezo.

Ibice byinyongera

• Pipettes:Ikoreshwa mugupima neza no kohereza amazi.
• Ibizamini by'ikizamini:Ikubiyemo ingero na reagent kugirango bigaragare.

Ibisubizo bya Buffer muri BSA bits

Uruhare rwa buffers

Buffers igira uruhare runini mugukomeza urwego rwa PH rukenewe kugirango bigaragare kubaho neza. Muri BSA bits, buffers ireba niba BSA nibindi bikoresho bikomeza guhagarara muburyo bugeragejwe.

Buffers isanzwe

Ifumbire isanzwe irimo fosifate - Buffered saline (PBS) na Tris Buffer, buriwese akoreshwa kugirango abone ibisabwa byihariye asabwa.

Ibizamini bya test na pipettes

Gukoresha ibizamini byikizamini

Ibizamini byikizamini bikoreshwa muburyo buvanze kandi akenshi byangwa nubunini bw'imbeba kubipimo byukuri. Igishushanyo cyabo nacyo cyemeza ko kuvanga bikwiye.

Precision hamwe na pipettes

Pipettes ningirakamaro kubinyomozo nyabyo no gukuraho amazi. Ukuri kwabo ni ngombwa mu gukomeza ubusugire bwibisubizo byubushakashatsi.

Ibishishwa no gukoresha

Reagents rusange

Reagents nka Dyes cyangwa Chromogenic subsrates zirimo koroshya amashusho yibisubizo. Izi mpandesha zikorana na BSA, zitanga ibara rihindura izo mpinduka hamwe na proteine.

Gufatanya

Gukora neza no kubika reagents ni ngombwa kugirango tumenye neza. Ibintu nkibitekerezo byoroheje cyangwa ubushyuhe bugomba gusuzumwa.

Imfashanyigisho n'ubuyobozi

Akamaro k'ibifungo

Igitabo cyigisha gikunze gushirwa muri BSA bikoresho kugirango kiyobore abakoresha binyuze muri gahunda igeragezwa, kureba ko intambwe zose zakurikiyeho kugirango utange ibisubizo byemewe.

Intambwe - na - Inzira nyababyeyi

Ibisanzwe mubisanzwe bitanga intambwe - na - Inzira yintambwe, inama zo gukemura ibibazo, nibisobanuro byamahame ya siyansi gashingiye kuri buri kigeragezo.

Ibikoresho byo kubika no kubungabunga

Imyitozo ikwiye yo kubika

Ibikoresho bya BSA akenshi bikubiyemo amabwiriza yihariye yo kubika, bishobora kuba bikubiyemo gukonjesha cyangwa kurinda urumuri, gukomeza ubusugire bwibice byitwa.

Inama zo kubungabunga

Kubungabunga buri gihe ibikoresho, nka Pipette Calibration, ningirakamaro kugirango wirinde amakosa yo gupima no kwemeza ko ibyavuye mubisubizo byubushakashatsi.

Itandukaniro rya bsa bits

Ubwoko bwa BSA Kits

BSA bits ziza muburyo butandukanye, buri kimwe cyagenewe porogaramu yihariye, nka poroteyine isanzwe ifasha, Imyombadite isaba, cyangwa inyongera yumuco. Ibi bikoresho birashobora gutandukana ukurikije kwibanda kuri BSA kandi harimo reagents.

Guhitamo Kit

Guhitamo ibikoresho bikwiye BSA biterwa nibisabwa hamwe nibisabwa byihariye byubushakashatsi, nko kumva no gusobanuka.

UMWANZURO: Guhitamo iburyo BSA

Guhitamo ibikoresho bya BSA nibyiza kugirango utsinde ubushakashatsi bwawe. Reba ibyifuzo nibisabwa nkubwoko bune, guhuza regent, nibisanzwe. Gufatanya numukozi wizewe, utanga isoko, cyangwa uruganda kugirango umenye neza ubuziranenge nubudahuriro rya BSA yawe.

Bluekit tanga ibisubizo

Bluekit, utanga isoko hamwe nuwabikoze mumahanga, atanga ibisubizo byuzuye bya BSA: Ibisubizo bya BSAT bikwiranye no gukenera laboratoire. Ibipimo byacu birimo hejuru - Isuku BSA Ibipimo, Ibisubizo byizewe Ibisubizo, hamwe nibikenewe byose, kwemeza neza kandi byoroshye kuri porogaramu zitandukanye. Umufatanyabikorwa ufite Ubururu bwo Kwizizizike Bwiza, inkunga ya tekiniki, hamwe nubuhanga butagereranywa, yaba akomoka mu ruganda rwacu cyangwa binyuze mubuguzi bwemewe. Hitamo bluekit kugirango wongere ubushishozi no gukora neza bya laboratoire yawe.