Intangiriro Kuri E.COLI Gutahura ADN ya ADN
FACOTNOLOKEK
Mu ntera ifatika ya biotechnology, gutahuraADNS, cyane cyane E.COLI ADN isigara, igaragara nkimpanuka ikomeye. Mugihe Ubwubatsi bwubuhanga na bioharmacedicace hamwe nimiti yo kubona imbaraga, kubungabunga ibicuruzwa byera biba umwanya munini. ADN ibinyabuzima bikomoka kuri E.COLI birashobora kuguma mubicuruzwa byanyuma, byoroshye ingaruka zibyara kandi zishobora gukurura ibishoboka byose bidafite ishingiro kubibazo. Ibi biba ngombwa cyane mumirima nkimikorere ya selire, aho kwanduza ADN bishobora guhungabanya imikorere numutekano wibicuruzwa byatangaga.
● Umutekano no Kumenyekanisha
Urwego rushinzwe kugenzura umurongo rutegeka urwego ruremewe rwa ADN rusigaye, kandi ababikora bagomba kubahiriza aya mabwiriza kugirango umutekano wibicuruzwa. Kubaho kwa ADN bisigaye mubicuruzwa nkikigo, antibodiyine za monoclon, na selile - Abavuzi bashingiye - Imbaraga zishingiye ku bicuruzwa zizibuka kandi zikagira ingaruka zikomeye zubuzima. Kubwibyo, uburyo bushya kandi bwizewe bwo gutahura no gushyiraho aba basinnye ntangarugero. Hamwe no kuzamuka mugusaba abavuzi b'akagari, ADN ibisigazwa ibisigazwa ni intambwe y'ingenzi mu kugenzura ubuziranenge, menyesha ubuziranenge n'umutekano by'intambwe yo kuvura.
Gusobanukirwa Ihame rya Taqman
● Gahunda yo gutahura
Gutabaza tekinoroji ya Taqman yahinduye uburyo buke bwa ADN. Ubu buryo bukoresha ibimenyetso byanditseho byerekana ko kuvangurana by'agateganyo runaka, koroshya amakuru asanzwe - igihe. Iyo Probe ihuza intego zayo mugihe cyongereranyo cya PCR, ibimenyetso bya fluorescent byasohotse, ugereranije kugeza kuri ADN ihari kurugero.
Uruhare mu gutahura E.COLI ADN
Taqman ikorwa neza cyane kugirango utange ibitekerezo e.coli dna itandukanye kubera umwihariko wabo no kwiyumvisha. Ibi bibazo birashobora gutandukanya ADN bifitanye isano ya ADN, kugenzura neza no kwinjiza. Ikoranabuhanga rya tekinoroji rituma igikoresho cyingenzi cya ADN gisizwe, abatanga ibitekerezo, nimpanga zigamije gukomeza amahame yo mu rwego rwo hejuru no kubahiriza ibicuruzwa byabo.
Ibyiza byo gukoreshaBluekit'stationt kit
● Leta - ya - - Ibiranga Ikoranabuhanga
Ibikoresho bya Bluekit bikubiyemo iterambere rigezweho mukoranabuhanga rya taqman, dutanga ibitekerezo bidahenze kandi neza mu kumenya E.Coli ADN ibisigisigi. Igishushanyo cya Kit cyemeza ko umusaraba muto - reactivite kandi umwihariko, ushishikajwe n'ibisubizo byizewe mu cyitegererezo kitoroshye. Uku gukata - Igisubizo cya Edge kijyanye no gutanga ibicuruzwa bya ADN hamwe nabakora ibisabwa basaba ubunyangamugayo no guhuzagurika muburyo bwabo.
Ssetivite itagereranywa no kwisobanura
Igikoresho cya Bluekit cyo kumenya Ubushobozi bwacyo bwo kumenya urwego ruto rwa ADN rusigaye, ruhuza ibisabwa na minerant byashyizweho n'abayobozi bashinzwe kugenzura. Ibyiyumvo byayo bifasha kumenya kwibanda kuri ADN nko hasi nka Picogramu 10, kureba no kwanduza gato byagaragaye kandi bikavugwa vuba.
Ibigize ibikoresho bya Bluekit
WITEGUYE - Kuri - Koresha Inyungu
Igikoresho cya Bluekit cyo gutahura kirimo kwitegura - kuri - Gukoresha imiterere, koroshya abakozi bangabimwe bya ADN hamwe na laboratoire. Iyi format igabanya umwanya wo kwitegura kandi igabanya ubushobozi bwo kwibeshya kwabantu, kuzamura imikorere mibi no kwizerwa. Kwinjiza mbere (Reagened Yemewe iremeza ibisubizo bihoraho kandi byororoka muburyo butandukanye.
● Kwinjiza umurongo usanzwe kugirango ukemeke
Ikintu kigaragara cyikintu cyubururu kivuga ni ugusohora umurongo usanzwe, ukomeye kugirango ugereranye neza na ADN neza. Gutanga amakuru asanzwe kumiterere itandukanye muri gahunda ya PCR, itanga ibisobanuro byizewe byo gusobanura ibisubizo. Ibi byemeza ko abakora dna basigaye basuzuma icyizere urwego rwo kwanduza ibicuruzwa byabo.
Guhitamo porogaramu ya QPCR na Porogaramu
Ubushakashatsi bwawe n'iterambere
Amasezerano ya Bluekit yo kumenya ibikoresho ni ibisubizo byubushakashatsi bwimbitse niterambere, bihujwe kugirango bibone ibikenewe bitandukanye bya ADN bisigaye bya ADN hamwe na porogaramu yo kuvura selire. Ibi bya protocole yoroshye byongera kumenya E.COLI ADN ibisigaye, byemeza ko abakora bashobora kubahiriza ibipimo ngenderwaho no kurinda umutekano wihangare.
● Guhinduranya muburyo butandukanye
Ibikoresho bya Kit bituma gushyira mubikorwa bitandukanye byubwoko bwibinini, uhereye mumirongo yibinyabuzima bigoye kugirango byegereje. Iyi mpinduka ifite agaciro cyane kuri ADN ibisigisigi bisigaye ikorana nibicuruzwa bitandukanye, kureba niba ubushobozi bwo gutahura butagarukira kubwicyitegererezo.
Kwiyumvisha no gutahura imipaka ya kit
● Kumenya umubare wa ADN
Ibikoresho bya Bluekit Gutahura bitanga ibyiyumvo bidasanzwe, bishoboye gutahura umunota wa ADN mubyitegererezo bigoye. Iyi sennitivite iremeza ko na e.coli ibisigisigi byamenyekanye, bigafasha abakora kugirango bakomeze urwego rwo hejuru rwitunganijwe numutekano.
Inama yo kugenzura ibipimo ngenderwaho
Kubahiriza amahame mbonezamubano mpuzamahanga nicyiza cya Dna Abatanga agaciro rya ADN, abakora, ninganda. Ubushobozi bwa kit yubururu bwubururu bwo kumenya ibisigaye bya DNN kurwego rwibikorwa cyangwa birenze ibyo bipimo byimyanya aho bigira inama yo kuyobora no kugenzura.
Hashyizweho umurongo wakazi kandi hagabanijwe kugabanya
Kugabanya ibishoboka byose
Igikoresho cya Bluekit cyo kumenya uburyo bwo kumenyana numukoresha wacyo - Igishushanyo cya gicuti, kugabanya ubushobozi bwikosa ryabantu. Inzira zikora hamwe namabwiriza asobanutse ayobora abakoresha binyuze kuri buri ntambwe, iremeza ibisubizo bihamye kandi byukuri. Uru rutonde rwumurimo ukorera inganda za DNA zisigaye mu rwego rwo kuzamura imikorere no kugabanya ibyago byamakuru atariyo.
● Gukora mu buryo bwo gutunganya
Gukora ibikorwa byo gutunganya ni ikintu gikomeye kuri ADN itanga ibisigazwa bigamije kuzuza igihe ntarengwa cyo kubyara. Ibisubizo bya Bluekit byo gutahura byihuse kandi byizewe bigabanya kugabanya no kwihuta - Gukora inzira, kwemerera abakora gutanga ibicuruzwa byiza ku isoko vuba.
Byihuse igihe cyo guhinduka hamwe ninyungu zisoko
Gutegura inzira yo gukora
Igihe cyihuse cyihuse cyatanzwe nigikoresho cya Bluekit cyo kumenya ibikorwa byo gukora, kwemerera abakora ADN kugirango bamenye kandi bakemure ibibazo byanduza vuba. Ubu buryo bworoshye bugabanya ubukererwe kandi bukomeza kubahiriza amahame yumutekano.
Igihe cyihuse - Kuri - Isoko ryibicuruzwa
Kubisigazwa bya ADN bisigaye hamwe nabakora selire, kugabanya igihe - to - Isoko ninkuge zikomeye zo guhatana. Igikoresho cya Bluekit cyo kumenya ibikoresho byoherejwe byihuse, bigatuma amasosiyete ahura no guhangayikishwa n akwiyongera no kubona ikirenge ku isoko vuba.
Kwipimisha no kwemeza ibice bya Kit
Kwipimisha imikorere
Ibigize ibikoresho bya Bluekit byo gutahura birimo gukora ibikorwa bikomeye kugirango wizere kandi uhumure. Ubu buryo bwo kwemeza neza ko buri kikoresho bwujuje ubuziranenge bwo hejuru, atanga ingengabihe ya DNA ifite ibikoresho byiringirwa no gutahura.
● Guharanira kwizerwa cyane no guhuzagurika
Kwizerwa no Guhuriza hamwe nicyiciro cya DNN gisiba gisigaranye gisigaye ku kikoresho cya Bluekit cyo kumenya neza. Mugukomeza ingamba zuzuye zuzuye, ibikoresho bitanga imikorere ihamye kumusazi byinshi, bikubise ikizere kubakora ndetse ninzego zigenga kimwe.
Umwanzuro nubuhamya bwabakoresha
● Kwizera no kunyurwa mu bakoresha
Igikoresho cya Bluekit cyandika cyarushijeho kwizera no kunyurwa mubakoresha, bigaragazwa nubuhamya bwiza bwakozwe na ADN usigaye abakora nabatanga isoko. Kwizerwa kwayo, gusobanurwa, no koroshya imikoreshereze bituma habaho guhitamo umutekano wibicuruzwa nubuziranenge.
● Kugenzura umutekano wibicuruzwa nibipimo byiza
Mugihe icyifuzo cya selile gishinzwe umutekano kandi cyiza gikomeje kuzamuka, uruhare rwa tekinoroji yateye imbere nkibikoresho bya Bludikit bitera byingenzi. Mugumya umutekano wimikorere no kubahiriza ibipimo ngenderwaho, ibikoresho bishyigikira ADN ibisiba bisigaye hamwe nababikora mugutanga hejuru - ubuziranenge, ibicuruzwa byiza, ibicuruzwa bitekamiye.
Ibyerekeye Bluekit
Bluekit ni ikirango gikomeye munsi ya Jiamsu Hillgene, icyicaro cya Suzhou, hamwe na GMP - Ibiti byemewe na centre ya R & D. Fliegene ikora imbuga zo gukora mugihugu hose kandi ikagura kwisi yose hamwe nurubuga muri Carolina y'Amajyaruguru. Inzobere mu kuvura selile, imisozi itezimbere urubuga rwa Acide Nuclec, Serum - Imico idahwitse, na QC igerageza tekinoroji. Ubururu bugaragaza uburyo bwiza bwo kugenzura guhanga udushya, gushyigikira iterambere ryimodoka - t, TCR - T, hamwe nubuvuzi bwa selile.
Kohereza Igihe: 2025 - 02 - 19 10:54:04
