Mu bihe byose - guhungabanya isi y'ibinyabuzima, ADN ya Plasmid yagaragaye nk'umukinnyi ukomeye, cyane cyane mu buvuzi bwa selire. Mugihe ubuvuzi bukomeje gusunika imipaka y'ibishoboka, uruhare rwa ADN rwa Plasmid mu iterambere no gushyira mu bikorwa abavuzi b'akagari ntibishobora gukandamizwa. Iyi ngingo isize mu isi ikomeye ya Dlasmid ADN ya Plasmid, isuzuma akamaro, imikorere yinganda, n'ingaruka zayo ku buvuzi bwa selire. Tuzareba kandi uruhare rwabakinnyi bakomeye mu nganda, tunerekana imisanzu ya disikuru ya Plasmid ADN, abatanga, ninganda. Hanyuma, tumenyekanisha isosiyete iyobora muriki kibanza,Bluekit, hanyuma muganire uburyo udushya twabo barimo gukora ejo hazaza h'umuvuzi.
Gusobanukirwa na plasmid ADN
DNA ni iki?
DNA ya PlasmidYerekeza kuri mato mato, uruziga rwa ADN ibaho yigenga ADN ya Chroyomal muri bagiteri hamwe na selile zimwe na zimwe. Iyi molekile ya dna yigana amafaranga yigenga kandi akenshi ikoreshwa nka rezo muri geneti yubuhanga kubera ubushobozi bwabo bwo gutwara ingirabuzimafatizo zabanyamahanga. Umutungo utihariye wa DALN wa Plasmid ubigira igikoresho ntagereranywa mumwanya wibi biotechnology, cyane cyane mugutezimbere imiyoboro ya genetike ninkingo.
Akamaro ka Plasmid ADN muri Biotechnology
ADN ya Plasmid igira uruhare runini mubuhanga bwa geneti, ikora nk'imodoka yo kwimura. Ubushobozi bwayo bwo kwigana yigenga butuma abashakashatsi batanga umusaruro mwinshi wa ADN, ushobora noneho kumenyeshwa mubindi binyabuzima. Ibi ni ingirakamaro cyane mumusaruro wa poroteyine zitangira, hashyirwaho ibinyabuzima byahinduwe na geneti, hamwe niterambere ryimyitwarire ya gene.
Dna ya plasmid muri kuvura selile
Uruhare rwa Dlasmid ADN mumitiri
Ubuvuzi bwa selire bukubiyemo ubuyobozi bwa selile nzima kumurwayi kuvura cyangwa kugabanya indwara. Intangiriro ya ADN ya Plasmid muriyi nzira yahinduye umurima, yemerera guhindura selile zerekana poroteyine zitandukanye. Ibi birashobora kuzamura imikorere yubuvuzi bwibintu bitandukanye, harimo na kanseri no kutavura kanseri.
Porogaramu ya Plasmid ADN yakozwe na selile
Kimwe mubyifuzo bya plasmid dna mubuvuzi bwumutwe ni imodoka - t (chimeric antigen reseptor t - selile) yo kuvura, kwivuza byihariye kuburyo bumwe bwa kanseri. Muri iki gikorwa, t - selile ziva mu murwayi, zahinduwe mu buryo busa na disikuru ya plasmid kugeza ingirabuzimafatizo za kanseri, kandi zigarurwa mu mubiri w'umurwayi gutera ingirabuzimafatizo. Ubuvuzi bwo hasi bwagaragaje intsinzi itoroshye mugufata kanseri yamaraso kandi irimo gushakishwa nubundi bwoko bwa kanseri.
Ibyiza byo gukoresha dna ya plasmid muri kuvura selile
Gukoresha ADN ya Plasmid mubuvuzi bwa selire itanga ibyiza byinshi:
Umwihariko no gukora neza: ADN wa Plasmid irashobora guhuza amabwiriza yihariye, yemerera intego nziza ya selile zirwaye.
Umutekano: Plasmids ntabwo ari abaganga bihujwe - Bihuza, bivuze ko batishyira hamwe muri genome yakiriye, kugabanya ibyago bya mutarusi no gukora imiti itekanye kubarwayi.
Ingutu: Gukora inzira ya Plasmid kuri Plasmid - Yashyizweho, yemerera Kinini - Umusaruro wumugezi kugirango ubone ibyo usabwa.
Inzira yo gukora ya Plasmid ADN
Incamake ya DASMID ADN Gukora
Umusaruro wa Plasmid ADN urimo intambwe nyinshi z'ingenzi, buri kintu cyingenzi kugirango ubone ibicuruzwa byanyuma nibicuruzwa. Iyi nzira isanzwe ikubiyemo ibyiciro bikurikira:
1.. Bimaze guhindurwa, iyi plasis yubatswe ukoresheje tekinike ya molekile.
2. Kwongererwa: Icyapa cyubatswe cyitangijwe muri selile zabakiriye bagiteri, aho bigana mugihe cyo kugabana akagari. Iyi ntambwe yongerewe ni ngombwa mugutanga byinshi bya Plasmid ADN.
3. Isuku: Iyo ubwinshi bwa DNN ya plasmid bwagezweho, irakuwe kandi isukurwa na selile za bagiteri. Iyi nzira ikubiyemo gukuraho imyanda ya selile nabanduye kugirango isuku.
4. Igenzura ryiza: Ingamba zo kugenzura ubuziranenge zishyirwa mu bikorwa kugirango igenzure ubunyangamugayo bwa Plasmid, ukurikirana ukuri, no kunyeganyega. Ibi byemeza ko DYNA ya plasmid yujuje ubuziranenge bukenewe bwo gukoresha.
INGORANE ZA Plasmid ADN
Mugihe inganda ya Dlasmid Amna yarushijeho kumvikana mumyaka yashize, ntabwo ari ibibazo. Ibintu nko gukomeza gukomera, kwemeza umusaruro mwinshi no kwemererwa, no gukumira umwanda ni ugukoresha ko abakora bagomba gukemura. Byongeye kandi, ibintu bigoye kubishushanyo mbonera kandi bikenewe ingamba zo kugenzura neza zituma iyi nzira yihariye.
Abakinnyi bakomeye mu nganda za DLASMID
Plasmid dna abakora, abatanga, ninganda
Icyifuzo cya Dlasmid ADN mu nganda zibinyabuzima zatumye habaho kugaragara kw'ababikora byihariye, abatanga, ningaga zeguriwe umusaruro wayo. Izi nzego zigira uruhare rukomeye muguharanira kuva hejuru - nziza ya plasmid ADN kugirango ikore ubushakashatsi na ivuriro.
Abakora DALNA bakora: Aya mashyirahamwe ashinzwe gutanga ADN ya Plasmid haba mu munzani gito kandi kinini. Bafite ubuhanga bwa tekiniki nibikorwa remezo bikenewe kugirango bakore ibigoye bya plasmid, byongerewe, no kwezwa.
Abatanga amakuru ya Plasmid: Abatanga isoko bakora nk'abahuza, bakwirakwiza ibigo by'ubushakashatsi mu bigo by'ubushakashatsi, ibigo bya biotech, n'ibikoresho by'amavuriro. Baremeza ko gutanga bya Plasmid bihamye byujuje ibikenewe byimpera zitandukanye - Abakoresha.
Inganda za Plasmid ADN: Inganda zeguriwe umusaruro wa Dlasmid ADN zifite ibikoresho bya leta - ya - - Ibikoresho byubuhanzi nibikoresho. Bakurikiza ibipimo ngenderwaho byo gukora kugirango habeho ubuziranenge bwibicuruzwa byinshi bya Plasmid.
Uruhare rwabasiwe ba SLA PLASMID mugutezimbere selire
Abatanga abatanga amakuru ya DLN bafite uruhare runini mu korohereza iterambere n'ubucuruzi bwa therapies. Mugutanga hejuru - nziza ya Plasmid, bafasha abashakashatsi nabaganga bakora ubushakashatsi bwimbitse kandi bwivuriro, bwihutiye guhindura imitsi ya selile kuva ku buriri.
UMWANZURO: Kazoza ka Plasmid ADN mubuvuzi bwa selire
Mugihe umurima wa biotechnologiya ukomeje gutera imbere, akamaro ko gucyapa bya plasmid mubuvuzi bwa selire bizakura gusa. Kugereranya kwayo, umutekano, hamwe nibikorwa byiza bigira igikoresho ntagereranywa cyo guteza imbere imiti mikuru yindwara. Ubufatanye hagati yabakora DALN'abi bakora, abatanga, ninganda ningirakamaro kugirango bahuze ibisabwa byiyongera kuri ibyo bicuruzwa no kureba neza ibishyirwa mubikorwa byakagari.
Intangiriro Kuri Bluekit
Jiangsu Hillgene, munsi yikirango cyayo bwubururu, gihagaze ku isonga rya ADN ya Plasmid na Torseling. Hamwe n'umuyoboro munini w'ibihingwa bya GMP hamwe na R & D muri Suzhou, Shenzhen, na Shanghai, no Gukomeza kwagura muri Carolina y'Amajyaruguru, Hillgene ni umuyobozi w'imikorere ya Acide. Ibicuruzwa byabo by'Abanyamerika bitanga gukata - Ibisubizo bya Edge yo kugenzura ubuziranenge, gushyigikira iterambere ry'imodoka - t, TCR - T, na selile ya Stem - Abavuzi bashingiye. Yiyemeje kwihutisha ubucuruzi bwa karubone, bluekit yeguriwe kunoza umusaruro wihanga binyuze mubisubizo bishya bikomoka mu binyabuzima.
Igihe cyagenwe: 2025 - 03 - 28 11:09:05
