Intangiriro
Umwanya wo kuvura selire wiboneye iterambere ridasanzwe mu myaka icumi ishize, cyane cyane mu kwagura no gushyira mu bikorwa selile zidasanzwe (NK). Aya selile idahumanze, ifite uruhare rukomeye muburyo bwo kwirwanaho bwumubiri, byabaye ibintu byinshi mugutezimbere imitekerereze ya kanseri kuri kanseri n'izindi mboherwa. Iyi ngingo isimburira ibintu byingenzi bya NK, uruhare rwa Trophoblast na Centre ya K562, akamaro ka CTOkines, n'akamaro ka Stokine, hamwe nubuyobozi buzaza kuri therapies. Byongeye kandi, dukora ku kamaro kaGusezera aho bikabas muguharanira ubuziranenge n'umutekano by'ibicuruzwa byo kuvura Akagari.
Incamake ya NK Kwagura Akagari
● Akamaro k'ubuvuzi
Ibikoresho bya NK byo kwagura ibikoresho bya NK nibikoresho bikomeye mu buvuzi bwa selire, bigafasha kongererwa selile za NK ku rwego rwo mu buvuzi. Ibi bikoresho byoroshya umusaruro wimibare ihagije ya NK ikenewe kubisabwa byubuvuzi, bityo bikamura ubushobozi bwabo bwo kuvura.
Ibigize ibikoresho
Mubisanzwe, kwaguka kwa NK KIts bigizwe nibice birimo ingirabuzimafatizo, Cytokines, hamwe nuburyo bwihariye bwa NK Akagari. Ibi bice bikora synergiste kugirango bikore ibidukikije byiza kuri fralifer ya NK
Uruhare rwa Trophoblast Ingirabuzimafatizo za NK
● Uburyo bwo gukangura
Ingirabuzimafatizo zo mu Trophoblast zisanzwe zikorana na selile ya NK mugihe utwite, ukina uruhare rukomeye muguhindura imikorere yabo. Mu rwego rwa NK kwaguka, Ingirabuzimafatizo zo mu Trophoblast zirashobora gukangura selile ya NK muri reseptor - ligand imikoranire, kuzamura ibikorwa byabo bya cytotoxique no kwibeshaho.
Inyungu zo gukoresha selile ya Trophoblast
Gukoresha Ingirabuzimafatizo zo Kwagura Inka ya NK IGHagura uburyo bwo kwagura physiologie kugirango utere imbere selile za NK, birashoboka ko ziganisha kuri selile zifite imikorere yubukorikori.
Moteri ya K562 muri NK kwaguka kwa NK
● Imvugo ya Cytokine muri K562
Momed K562 ikoreshwa kenshi nka selile mbi muri NK kwaguka kwa Kagari. Bahinduwe kugirango bagaragaze Cytokines nka Il - 15 na Il - 21, ni ngombwa kuri nk imikurire ya NK no gukora.
Ingaruka zo kurakara no kudakora
Irradiation cyangwa kudakora ingirabuzimafatizo za K562 mbere yo gukoreshwa nka selile zizabera umutekano mukubabuza kwibeshaho kudashaka, mugihe ugigumana ubushobozi bwo gushyigikira kwangwa na NK.
Akamaro ka CTOkines mu gukora NK
● Il - 21 Ingaruka zayo
Cytokine nka IL - 21 ni ibintu bifatika kuri NK selile. Il - 21 Ntabwo ari iteza imbere gusa irangi rya NK gusa ahubwo rinahugura ibikorwa byabo bya cytotoxique, bikora ikintu cyingenzi muri kits ya NK.
● Inzira ya Synorgistist
Cytokines akora binyuze muburyo butandukanye bwo kwerekana kugirango bakore selile NK. Gusobanukirwa muriyi nzira ningirakamaro kugirango utegure protocole ya NK no kunoza imikorere yabo ya therapeutic.
Inkomoko ya NK: Umumbatsi wumugozi namaraso ya periphel
Kugereranya umusaruro wa selire
Amaraso yumugozi nisoko gakize yingirabuzimafatizo za NK, akenshi itanga imibare nkuru ugereranije namaraso ya periphel. Ariko, guhitamo hagati yibi bicuruzwa biterwa nibisabwa byihariye bya porogaramu yo gutangaza.
● Isuku nubwiza bwa NK na selile
Amakuru yombi arashobora gutanga hejuru - Ingirabuzimafatizo za NK, ariko protocole yo kwaguka irashobora gutandukana. Kwemeza ubuziranenge n'imikorere ya NK yagutse NK irakomeye kubisubizo byifashe neza.
Gusaba mumodoka - NK Kwitegura Akagari
Gutunganya Gukura Imodoka - NK selile
Imodoka - NK selile zakozwe kugirango ugaragaze abakiranirwa na chimeric antigen, ubashobore kwibasira selile zihariye za kanseri. Gutegura imodoka - Ingirabuzimafatizo zirimo guhinduranya no kwaguka, inzira zorohereza ibikoresho byihariye.
● Ibyiza hejuru yuburyo gakondo
Imodoka - NK selile zitanga inyungu nyinshi zibimenyeshwa gakondo, harimo no kugabanya ibyago byo kuzenguruka - na - Indwara ya Wakira nubushobozi bwo kwibasira intera ya kanseri.
Guteza imbere gahunda yo kuvura selire
● Igihe no Gutiza imikorere mugutezimbere hakiri kare
Gutezimbere inzira nziza ya NK selile selile ningirakamaro mugihe cyo kugabanya igihe n'ibiciro mu byiciro byiterambere hakiri kare. Gutegura protocole no kugoreka kwitegura - Kuri - Koresha ibikoresho byo kwagura birashobora gukora cyane iyi nzira.
● Ingamba zo guhitamo ibicuruzwa bya selire
Ingamba nko kwikora, gutunganya sisitemu ifunze, hamwe no gukoresha mikorobial - reagents yubusa ni ngombwa muguhitamo ubuziranenge no guhuza ibicuruzwa bya selire.
Kuzamura isuku no gutanga selile ya NK
Tekinike Kugera ku Isuku
Tekinike nko gutembera kwometry - Gutandukanya Gutandukanya no Gukwirakwiza Magnetic bikoreshwa kugirango ugere ku nzego zisukuye muri selile zagutse muri selile zagutse, ni ingenzi cyane kubisaba amavuriro.
● Inzitizi n'ibisubizo muri binini - Kwagura Igipimo
Gupima NK Kwagura Akagari bitera ibibazo nko kubungabunga selile kabyutse no gukora. Ibishushanyo bishya bya Bioreative hamwe na sisitemu yo gukurikirana ikomeza nibisubizo kuri izi mbogamizi.
Inyungu zo Gukoresha Ubugari bwa NK Akagari
Uruhare mu kwaguka kwa Kagari no Gukora
Uburyo bwa NK Akagari kateganijwe gushyigikira iterambere no gukora selile ya NK, kwemeza imikorere ihamye ahantu hatandukanye kwaguka.
Guhuza na Ctokine zitandukanye
Uburyo bwo guhuza urwego rwinshi bwa Ctokine yemerera guhinduka muguhana kwa NK kugirango ihuze ibikenewe byihariye.
Icyerekezo kizaza kuri NK Akagari ka NK Akagari
● Inzira igaragara muri kuvura selire
Umwanya wa NK Umuvugizi wa NK uragenda wiyongera, hamwe no kurebera byihuse byibanda ku kuzamura umwihariko, bigabanya - ingaruka zintego, no kuzamura igitero cy'ibijumba.
Iterambere rishobora kuba muri gahunda ya NK
Iterambere ry'ejo hazaza rishobora kuba rikubiyemo iterambere ryabaterankunga nK rusange, imiterere ya hamwe nandi kagari k'umuntu udahumanywa, kandi imyigaragambyo ya NK ya selile ijyanye nimwiyubatozo ku giti cyabo.
Guharanira ubuziranenge hamwe na selile ya dna yateguwe
● Akamaro k'ubuvuzi
Ikigo cyakira selile cya DNA nibyingenzi mubuvuzi bwa selire kugirango utange kandi ugereranye na selile yakiriye. Ibi bikoresho byemeza umutekano nibikorwa byibicuruzwa byo kuvura selire mugugabanya ibyago byo kwanduza.
Uruhare rw'abakora n'abatanga isoko
Abakora nabatanga ibicuruzwa byakiriye dna byateguwe ibikoresho bya DNN, nkaBluekit, ukine uruhare rukomeye mugutanga ibikoresho byizewe kandi hejuru - Ibikoresho byiza. Ibi bikoresho ni ngombwa mu gukomeza ubusugire bwumusaruro wo kuvura.
Umwanzuro
Ahantu h'ubuvuzi bwa selire bikomeza guhinduka, hamwe na selile ya NK ku isonga ryiyi mpinduramatwara. Kwishyira hamwe kw'ibikoresho byagutse byagutse, Sitokine ubushishozi, hamwe n'ibikoresho by'ubuziranenge bwemewe na Wast Ibikoresho bya Askrocessing bitwara iterambere ry'imibare myiza kandi myiza. Mugihe inganda zigenda imbere, ubufatanye hagati y'abashakashatsi, abaganga, n'abakora bazaba urufunguzo rwo gufungura ubushobozi bwuzuye bwa orrapies.
Jiagsu Hillgene, munsi ya Bluekit®, ahagarara ku isonga rya selire yo kuvura udushya. Hamwe n'icyicaro cyayo muri Suzhou no mu mbuga zo mu Bushinwa na Carolina y'Amajyaruguru, Hillgene ni ubupayiniya bwo gukora ubupayiniya mu mwobora no mu rwego rwo kuvura ibicuruzwa. Yiyemeje kugenzura ubuziranenge, ibicuruzwa by Bluekit® byemeza umutekano n'imiterere ya selile, ishyigikira abafatanyabikorwa ku isi mu kugera ku modoka yatsinze - t, TCR - T, n'ibicuruzwa bishingiye ku giti cye. Ibicuruzwa bishingiye ku giti cye. Hamwe n'icyerekezo cyo kuvura udushya, Hillgene yeguriwe gutera imbere ibisubizo by'itubutagenda neza ku isi hose.
Igihe cyagenwe: 2024 - 12 - 13 15:31:09
