Nk ndi Cell Cell Cell (K562 TEVER)
Nk ndi Cell Cell Cell (K562 TEVER)
✔ Prerad, mitundu yambiri - cytokine yolimbikitsidwa kuti ikhale yayikulu ya NK / yayitali.
✔ Zovomerezeka kwa Magazi ndi Pbmc - Galimoto - okonzeka.
✔ Pre - oyenerera kutumiza masana omwe ali ndi chidziwitso chonse.
Mwachidule:
A NK / Til Kukula Kosanja ili ndi Wopangidwa, wolemera K562 TV kufotokoza IL - 21 ndi ma cytokines ena ofunikira. Mukaphatikizidwa ndi Media State Media, dongosolo lino limathandiza:
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Mkulu - Kuchuluka kwamphamvu wa Ma cell a NK ndi tils Kuchokera ku PBMCS kapena Magazi -
Mkulu - Matumba Oyera (> 90% ma cell)
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Mwachangu, oyambitsa pansi pa zikwangwani zotsekemera
Ntchito zazikulu
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Kuyambitsa & kukulitsa wa Maselo a NK, galimoto - zingwe, ndi tils
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Kukula kwa magwiridwe: Koyambirira - Kutsanzira kwa Cell mankhwalawa
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GMP KUSINTHA: Zothandiza Ind - Kuthandizira maphunziro ndi Post - Kuvomerezedwa Kusintha
Regilatory & Magwiridwe Abwino
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GMP - Otsatira kapangidwe
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Zogwirizana, zapamwamba - Kukula Kwatsopano (mpaka 1000 - Pindani)
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Pre - Otsimikizika pakugwiritsa ntchito mankhwala
Tsamba lazambiri
Kuchulukitsa kwa cell kwa NK
Wopereka - to - Opereka Vanaliality mu Chiyero cha NK
Mphamvu yowonjezera ya virul E - Hancerc pa entivil transctionction mankhwala a NK
Kutulutsa kwa cell cell: Virul E - Hancerc Opperforms ku Moi 1 Panel: Kutulutsa cytometry ya IL - NK maselo okhala ndi virul E - Hancerc
Nyanja kumanja: Kutulutsa cytometry ya IL - ma cell 15 a NK popanda kuthira
Virul E - Hancerc vs. opikisana: T - Kutumiza kwa cell ku Moi 5 Kumanzere: Tsitsani cytometry yagalimoto - TORD ndi Virul E - Hancerc
Kumanja: Tsekani cytometry yagalimoto - T] Maselo osagwiritsa ntchito
Kupititsa patsogolo mphamvu za vil E - Hancer a + viral E - Hancer D Kuphatikiza pa lentiviras transiction mu HSCES
Kusanthula kofananira kwa mphamvu ya HSS kutumizidwa: Virul E - Hancera + HARIL E - Kuphatikizika kwa Scritors (Moi = 5)
| Nambala ya siriyo | Dzinalo la Zovuta Zovuta | Mlingo (wa ~ 1 - 2E6 pbmc choyambitsa) |
| 1 | Seramu - mfulu yaulere | 2 l |
| 2 | Il - 2 | Mamasi 4 (200,000 IU / Ndodo) |
| 3 | 0,9% jacl jacker | ~ 500 ml |
| 4 | 20% ya anthu a Albumin | ~ 15 ml |
| 5 | Ficoll lymphocyte yankho | ~ 25 ml |
| 6 | NC - Kuwerengera Chambert (kapena chofanana) | Pofunika |
| 7 | 6 - mbale | 1 |
| 8 | T25 Flask | 1 |
| 9 | T75 Flusk | 1 |
| 10 | T175 Flask | 2 |
| 11 | 640 masentimita | 2 |
| 12 | 15 ml centrifuge chubu chubu | 1 |
| 13 | 1.5 ml centrifuge chubu | Pofunika |
| 14 | 10 ml / 25 ml mapaipi | Pofunika |
| 15 | 1 ml / 200 μl pipette malangizo | Pofunika |
Chifanizo
| Parament | Chifanizo |
| NK / Car - NK Clain | > 5,000 × (masiku 14) |
| Kuchita bwino kwa maselo | ≥90% |
| Chiyero cha m'manja (cd56⁺ / CD3⁻) | > 95% |
Kutumiza Zambiri
Timapereka kunyamula mayendedwe onse pamalamulo onse. Nthawi zambiri, oda yanu ifika mkati mwa 5 - masiku 7 a bizinesi ku United States komanso mkati mwa masiku 10 antchito a mayiko ena. Komabe, chonde dziwani kuti kupembedza kumidzi ingatenge nthawi yayitali.
Nthawi Yotumiza: Maodawo amakonzedwa mkati mwa 1 - masiku atatu antchito. Dongosolo lanu litatumizidwa, mudzalandira imelo yotsimikizira ndi chidziwitso chotsatira.
Chidziwitso chofunikira
Kukonzanso: Kulamula kwaperekedwa, nyumba yathu yosungirako ikusowa nthawi kuti akonze dongosolo lanu. Mudzalandira chidziwitso kamodzi oda yanu yatumizidwa.
Nthawi zoperekera: Nthawi zambiri, phukusi lidzaperekedwa mkati mwa nthawi yomwe ikuchitika. Komabe, tsiku lenileni la zoperekera lingakhudzidwe ndi makonzedwe, nyengo, ndi zinthu zina zakunja. Nthawi yoperekera idzakhala yotalikirapo kuposa masiku onse okhudzana ndi malo ophatikizika kapena zinthu zosinthidwa. Chonde onani chidziwitso chotsatira tsiku lolondola kwambiri.
Nkhani Zotumiza: Ngati mukuwona kuti phukusi lanu silinaperekedwe mkati mwa nthawi yodziwika; Zambiri zotsatila zikuwonetsa kuti phukusi laperekedwa koma simunalandire; kapena phukusi lanu limaphatikizapo zinthu zosowa kapena zolakwika kapena zovuta zina, chonde lemberani kasitomala mkati mwa masiku 7 a tsiku lolipira kuti tithe kuthana ndi mavutowa mwachangu.
Kutsatira kutsatira
Dongosolo lanu litatumizidwa, mudzalandira imelo ndi nambala yolondola komanso ulalo kuti muthe kutumiza.
Mutha kutsatanso dongosolo lanu mwachindunji patsamba lathu podula muakaunti yanu ndikuwona mbiri yanu.
Zoletsa Kutumiza
Chonde lembani adilesi yamsewu mwatsatanetsatane, osati bokosi kapena adilesi yankhondo (apo). Kupanda kutero, tiyenera kugwiritsa ntchito EMS kuti tibweretsenso ena (amayamba pang'onopang'ono kuposa ena, kutenga 1 - miyezi iwiri kapena kupitilira).
Ntchito Zogwirira Ntchito ndi Msonkho
Chonde dziwani kuti ntchito zilizonse zamitundu, misonkho, kapena zolipirira zobwereketsa zomwe zikugulitsidwa ndi udindo wa wogula. Zolipira izi zimasiyana kutengera dzikolo ndipo zimatsimikizika ndi akuluakulu akwanuko am'deralo.
Pogula kuchokera patsamba lathu, mukuvomera kulipira ntchito kapena misonkho yomwe imagwirizana ndi dongosolo lanu. Sitili ndi vuto la kuchedwa chifukwa cha chilolezo cha miyambo.
MALANGIZO OTHANDIZA
Dongosolo lanu litafika pamalo osankhidwa kapena malo operekera, chonde onetsetsani kuti ikutola. Ngati phukusi silinatengedwe mkati mwa nthawi yomwe yasankhidwa, tidzakutumizirani chikumbutso kudzera pa imelo kapena SMS. Komabe, ngati phukusi silikusonkhanitsidwa nthawi yayitali, ndipo kutayika kapena kuwonongeka kulikonse kumachitika chifukwa, wogula azikhala ndi mlandu. Tikukumbutsani mokoma kuti mutole phukusi lanu mwachangu kupewa nkhani zilizonse zomwe zingachitike.
Chidziwitso: Pamene malonda athu amagwera pansi pa gulu lapadera, amabwerera ndi kubweza sikuvomerezedwa.
