Lentivirus Tited P24 RED RED ELISSA YOPHUNZITSIRA
Lentivirus Tited P24 RED RED ELISSA YOPHUNZITSIRA
✔ Kuphatikiza ndi tu / ml (1 NG P24 ≈ 1 × 10
✔ 21 cfr gawo 11 - okonzeka Ma templation a zamagetsi amaphatikizidwa
✔ Kuchira kwa Spike 92 - 108% mu zitsanzo za LV
Mwachidule:
Chingwe chimagwiritsa ntchito a sangweji Elisa Njira ya hiv - 1 P24 Kuchulukitsa:
Microplate yokutidwa ndi anti - p24 monoclonal antibody
Mafomu antiody - antigen - antigen antib holed pa RT infibution
TMB gawo la TMB limatulutsa mawonekedwe ofanana ndi p24
Zolemba:
|
Palamu |
Chifanizo |
|
Mitundu yamphamvu |
1.37-1000 ng / ml |
|
Makulidwe (LoD) |
0.35 NG / ml |
|
Chidule |
Intra - Assay Cv <10% |
|
Kulunjika |
85-115% Kubwezeretsa |
Zofunikira:
Mwachangu 45 - min protocol (Rt incubation)
Okonzeka - ku - - - - - - gwiritsani ntchito ma reagents
Kuphatikiza ndi Irier Char .
Curve Standard:
Tsamba lazambiri:
Kodi pabokosi?
| Chipangizo | Chifanizo | Kusunga |
| Mbale yokutidwa | 1 × 96 Wells | Kutentha kwa chipinda |
| Anti - HIV - 1 P24 - hrp conjugate | 6 ml | 2 - 8 ° C |
| HIV - 1 P24 Wokhazikika | S0 - S7 (Miyezo: " | 2 - 8 ° C |
| Virus lysis buffer | 6 ml | 2 - 8 ° C |
| Chitsanzo Choseketsa | 50 ml | Kutentha kwa chipinda |
| 10 × pbst sambani buffer | 50 ml | Kutentha kwa chipinda |
| TMB gawo | 12 ml | 2 - 8 ° C |
| Siyani yankho | 12 ml | Kutentha kwa chipinda |
| Plate | 5 | Kutentha kwa chipinda |
| Buku la malangizo | 1 | N / A |
chifanizo
|
Mtundu |
1.37 - 1000 ng / ml |
|
Kuzindikira kumvetsetsa |
0.35 NG / ml |
|
Chidule |
CV% ≤ 10%, re% ≤ ± 15% |
Kutumiza Zambiri
Timapereka kunyamula mayendedwe onse pamalamulo onse. Nthawi zambiri, oda yanu ifika mkati mwa 5 - masiku 7 a bizinesi ku United States komanso mkati mwa masiku 10 antchito a mayiko ena. Komabe, chonde dziwani kuti kupembedza kumidzi ingatenge nthawi yayitali.
Nthawi Yotumiza: Maodawo amakonzedwa mkati mwa 1 - masiku atatu antchito. Dongosolo lanu litatumizidwa, mudzalandira imelo yotsimikizira ndi chidziwitso chotsatira.
Chidziwitso chofunikira
Kukonzanso: Kulamula kwaperekedwa, nyumba yathu yosungirako ikusowa nthawi kuti akonze dongosolo lanu. Mudzalandira chidziwitso kamodzi oda yanu yatumizidwa.
Nthawi zoperekera: Nthawi zambiri, phukusi lidzaperekedwa mkati mwa nthawi yomwe ikuchitika. Komabe, tsiku lenileni la zoperekera lingakhudzidwe ndi makonzedwe, nyengo, ndi zinthu zina zakunja. Nthawi yoperekera idzakhala yotalikirapo kuposa masiku onse okhudzana ndi malo ophatikizika kapena zinthu zosinthidwa. Chonde onani chidziwitso chotsatira tsiku lolondola kwambiri.
Nkhani Zotumiza: Ngati mukuwona kuti phukusi lanu silinaperekedwe mkati mwa nthawi yodziwika; Zambiri zotsatila zikuwonetsa kuti phukusi laperekedwa koma simunalandire; kapena phukusi lanu limaphatikizapo zinthu zosowa kapena zolakwika kapena zovuta zina, chonde lemberani kasitomala mkati mwa masiku 7 a tsiku lolipira kuti tithe kuthana ndi mavutowa mwachangu.
Kutsatira kutsatira
Dongosolo lanu litatumizidwa, mudzalandira imelo ndi nambala yolondola komanso ulalo kuti muthe kutumiza.
Mutha kutsatanso dongosolo lanu mwachindunji patsamba lathu podula muakaunti yanu ndikuwona mbiri yanu.
Zoletsa Kutumiza
Chonde lembani adilesi yamsewu mwatsatanetsatane, osati bokosi kapena adilesi yankhondo (apo). Kupanda kutero, tiyenera kugwiritsa ntchito EMS kuti tibweretsenso ena (amayamba pang'onopang'ono kuposa ena, kutenga 1 - miyezi iwiri kapena kupitilira).
Ntchito Zogwirira Ntchito ndi Msonkho
Chonde dziwani kuti ntchito zilizonse zamitundu, misonkho, kapena zolipirira zobwereketsa zomwe zikugulitsidwa ndi udindo wa wogula. Zolipira izi zimasiyana kutengera dzikolo ndipo zimatsimikizika ndi akuluakulu akwanuko am'deralo.
Pogula kuchokera patsamba lathu, mukuvomera kulipira ntchito kapena misonkho yomwe imagwirizana ndi dongosolo lanu. Sitili ndi vuto la kuchedwa chifukwa cha chilolezo cha miyambo.
MALANGIZO OTHANDIZA
Dongosolo lanu litafika pamalo osankhidwa kapena malo operekera, chonde onetsetsani kuti ikutola. Ngati phukusi silinatengedwe mkati mwa nthawi yomwe yasankhidwa, tidzakutumizirani chikumbutso kudzera pa imelo kapena SMS. Komabe, ngati phukusi silikusonkhanitsidwa nthawi yayitali, ndipo kutayika kapena kuwonongeka kulikonse kumachitika chifukwa, wogula azikhala ndi mlandu. Tikukumbutsani mokoma kuti mutole phukusi lanu mwachangu kupewa nkhani zilizonse zomwe zingachitike.
Chidziwitso: Pamene malonda athu amagwera pansi pa gulu lapadera, amabwerera ndi kubweza sikuvomerezedwa.
