Cell cytotoxicity istay Kit (ma cell contrant)
Cell cytotoxicity istay Kit (ma cell contrant)
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✔ CFSE - Ma cell a Center Orft of Facy Kuzindikira
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- 7 - AAD Kukhazikika kosintha
✔Adapangidwa kuti aletse mizere yotupa
Mwachidule:
Izi zidapangidwa kuti zitheke Kugwiritsa ntchito cell cytotoxicity motsutsana ndi ma cell, zoyenera Maselo a NK, galimoto - maselo a nk, galimoto - ma t - tcr - ma cell, ndi ma cell tm chotupa - kupha mabodza.
Zigawo za Kit:
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Cfse .
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7 - ad (7 - Aminoctomycin D, DNA - utoto womanga ma cell ale wakufa)
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Wapakati b .
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Stay buffer (Akuwonetsetsa kuti asintha
Ntchito zazikulu
✔Kuyeserera kwa Ntchito za mthupi lam'mandende (E.g., galimoto - T, maselo a NK) motsutsana chotupa chotupa cha cell
✔Kukonzekera Kukula & Kuthamangitsidwa mu kafukufuku wa celly
✔Kumasula mayeso & maphunziro okhazikika kwa gmp - Kupanga
Zochita:
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Kulemba kwapadera (A CFSE NGATI NAWU
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Protocol yokhazikika Zotsatira Zobala
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Yogwirizana ndi Rid - Mpukutu (96/384 - Mitundu Yabwino)
Remortory & Makampani Otsatira
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Zovomerezeka mizere yotupa yama cell (E.g., Sela, A549, MCF - 7)
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Zoyenera Ind - Kuthandizira maphunziro (Ich Q2 (R1) yogwirizana)
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21 cfr gawo 11- Kukonzekera kufotokozera kwa deta
NK - Kupha Komwe kwa CFSE + A549 Kutulutsa Cytometry
NK - Kupha kwa CFSE + kwa zotupa zolimba za cytometry
Tsamba lazambiri:
| Mycoplasma mavuto | Zabwino / zokwanira | Masitima ogwirizana kwambiri | Zabwino / zokwanira | Lactobaclus Allemichilhils | Streptocococcus chibayoe | Clostridium Acetututylicum |
|---|---|---|---|---|---|---|
| Mycoplasma orale | 24/24 | Mycoplaskuma arhomphodis | 24/24 | Wosavomela | Wosavomela | Wosavomela |
| Mycoplasma gulsepticulam | 23/24 | Mycoplasma arianinini | 24/24 | Wosavomela | Wosavomela | Wosavomela |
| ACHALPLASMA Laidolaii | 24/24 | Mycoplasma hyorhinis | 24/24 | Wosavomela | Wosavomela | Wosavomela |
| Mycoplasma fermentans | 23/24 | Snolal'ma Citri | 24/24 | Wosavomela | Wosavomela | Wosavomela |
| Mycoplasma chibayoe | 24/24 | Mycoplasma salivarium | 24/24 | Wosavomela | Wosavomela | Wosavomela |
| Catalog No. | Chipangizo | Chifanizo | Kutentha | Malo |
| HG - MD002 | Wapakati b | 100 ml | 2 - 8 ° C | Kit i |
| HG - FW001 | Stay buffer | 100 ml | 2 - 8 ° C | - |
| HG - CFSE | Cfse | 1 vial | - 20 ° C | Kit II |
| HG - 7 - AAD | 7 - ad | 1 vial | - 20 ° C | - |
Chifanizo
| Parament | Kuvomerezedwa |
| Kulunjika | 70-130% Kuchira |
| Kulimba | 70-130% coas |
| Kuzengeleza | Cv <10% |
| Pakatikati | CV ≤ 10% |
Kutumiza Zambiri
Timapereka kunyamula mayendedwe onse pamalamulo onse. Nthawi zambiri, oda yanu ifika mkati mwa 5 - masiku 7 a bizinesi ku United States komanso mkati mwa masiku 10 antchito a mayiko ena. Komabe, chonde dziwani kuti kupembedza kumidzi ingatenge nthawi yayitali.
Nthawi Yotumiza: Maodawo amakonzedwa mkati mwa 1 - masiku atatu antchito. Dongosolo lanu litatumizidwa, mudzalandira imelo yotsimikizira ndi chidziwitso chotsatira.
Chidziwitso chofunikira
Kukonzanso: Kulamula kwaperekedwa, nyumba yathu yosungirako ikusowa nthawi kuti akonze dongosolo lanu. Mudzalandira chidziwitso kamodzi oda yanu yatumizidwa.
Nthawi zoperekera: Nthawi zambiri, phukusi lidzaperekedwa mkati mwa nthawi yomwe ikuchitika. Komabe, tsiku lenileni la zoperekera lingakhudzidwe ndi makonzedwe, nyengo, ndi zinthu zina zakunja. Nthawi yoperekera idzakhala yotalikirapo kuposa masiku onse okhudzana ndi malo ophatikizika kapena zinthu zosinthidwa. Chonde onani chidziwitso chotsatira tsiku lolondola kwambiri.
Nkhani Zotumiza: Ngati mukuwona kuti phukusi lanu silinaperekedwe mkati mwa nthawi yodziwika; Zambiri zotsatila zikuwonetsa kuti phukusi laperekedwa koma simunalandire; kapena phukusi lanu limaphatikizapo zinthu zosowa kapena zolakwika kapena zovuta zina, chonde lemberani kasitomala mkati mwa masiku 7 a tsiku lolipira kuti tithe kuthana ndi mavutowa mwachangu.
Kutsatira kutsatira
Dongosolo lanu litatumizidwa, mudzalandira imelo ndi nambala yolondola komanso ulalo kuti muthe kutumiza.
Mutha kutsatanso dongosolo lanu mwachindunji patsamba lathu podula muakaunti yanu ndikuwona mbiri yanu.
Zoletsa Kutumiza
Chonde lembani adilesi yamsewu mwatsatanetsatane, osati bokosi kapena adilesi yankhondo (apo). Kupanda kutero, tiyenera kugwiritsa ntchito EMS kuti tibweretsenso ena (amayamba pang'onopang'ono kuposa ena, kutenga 1 - miyezi iwiri kapena kupitilira).
Ntchito Zogwirira Ntchito ndi Msonkho
Chonde dziwani kuti ntchito zilizonse zamitundu, misonkho, kapena zolipirira zobwereketsa zomwe zikugulitsidwa ndi udindo wa wogula. Zolipira izi zimasiyana kutengera dzikolo ndipo zimatsimikizika ndi akuluakulu akwanuko am'deralo.
Pogula kuchokera patsamba lathu, mukuvomera kulipira ntchito kapena misonkho yomwe imagwirizana ndi dongosolo lanu. Sitili ndi vuto la kuchedwa chifukwa cha chilolezo cha miyambo.
MALANGIZO OTHANDIZA
Dongosolo lanu litafika pamalo osankhidwa kapena malo operekera, chonde onetsetsani kuti ikutola. Ngati phukusi silinatengedwe mkati mwa nthawi yomwe yasankhidwa, tidzakutumizirani chikumbutso kudzera pa imelo kapena SMS. Komabe, ngati phukusi silikusonkhanitsidwa nthawi yayitali, ndipo kutayika kapena kuwonongeka kulikonse kumachitika chifukwa, wogula azikhala ndi mlandu. Tikukumbutsani mokoma kuti mutole phukusi lanu mwachangu kupewa nkhani zilizonse zomwe zingachitike.
Chidziwitso: Pamene malonda athu amagwera pansi pa gulu lapadera, amabwerera ndi kubweza sikuvomerezedwa.
