Kodi pali kusiyana kotani pakati pa trypsin ndi ma proteases ena?

Mafala Akutoma Nawo Kuchilengedwe

Mapuloteke, gulu losiyanasiyana la ma enzymes, ndizofunikira njira zambiri zachilengedwe, chifukwa cha chimbudzi kupita ku magonedwe. Amagwira ntchito ndi kupatsana kwa zomangira za Peptide m'matete mapuloteni, motero amasewera gawo lofunikira pakusunga ma cell hometostasis. Mapuloteke amasankhidwa chifukwa cha njira zawo zothandizira komanso zopindika kwambiri, ndi mapuloteni a seriji ngati trypsin kukhala m'gulu lomwe amaphunziridwa kwambiri. Nkhaniyi ikuwunika zinthu zina zapadera za trypsin mosiyana ndi mapuloteni ena, poganizira zinthu zina, magwiridwe antchito, magwiridwe antchito, ndi ntchito.

Trypsin: Muyezo wagolide wa proteomics

Udindo wa Trypsin mu Mass Spertmetry

Trypssin imatsogolera gawo la mapuloteni chifukwa cha kupezeka kwake kwakukulu ndi luso loyeretsa maunyolo papepala pa carboxyl mbali ya lysine ndi arginine. Izi zimabweretsa zidutswa zokhala ndi mtengo wokwanira ku C - Malumiya, opindulitsa kwambiri pamiyeso yambiri (ms). Kugwiritsa ntchito mapuloteni, kuthekera kwa trypsin kutulutsa mapepu okwanira kumathandizira kuti mapuloteni ayambe kufufuza kafukufuku wa cell, komwe mawonekedwe olondola a protein ndikofunikira.

Ubwino wa Kuzindikira Kafukufuku

Ma protecics ambiri amadalira katundu wa trypsin ya kuzindikiritsa mapuloteni. Kafukufuku akuwonetsa kuti tryspsin amatha kudziwa zogulitsa zoposa 80% poyerekeza ndi mapuloteni ndi mapuroteni ochulukirapo, ndikupangitsa kuti zikhale zofunika kwambiri, ndikupangitsa ofufuza ndi othandizira m'munda. Udindo wake umakula kuchokera ku matenda omvetsetsa njira zolimbikitsira njira zochimwa, monga mankhwala a cell.

Machitidwe a trypsin

Malo ogwiritsiridwa ndi magwiridwe othandizira

Ntchito yothandiza ya trypsin imayambira pachitsime chake - Pofotokoza za malo ogwirizira, zopangidwa ndi catalytic thiad wa seriine, histdine, ndi aspartete. Kusintha kumeneku kumathandizira kuti pakhale chiwonetsero chokhazikika, chofunikira kwambiri kwa ogulitsa omwe amapereka miyambo yoyeretsa yofufuzira ndi mafakitale. Chinsinsi cha enzyme chimadziwika kuti kupezeka kwa chotsimikizika cha asparte chotsalira, chomwe chimakopa lysine ndi arginine.

Ma iroforms a trypsin

Tryssin alipo mu iyofoorm angapo, kuphatikiza cutic, anionic, ndi mesotrypsinogen. Iroform iliyonse imapereka maudindo osiyanasiyana, ndikupanga katswiri wojambula kwambiri kukhala wochuluka kwambiri mwa kapamba wa munthu. Mesotrypysprin ndi wodziwika chifukwa chokana zoletsa, kusewera gawo lapadera pakuwonongeka kwa inrobitor. Kuzindikira zinthuzi ndikofunikira kuti mafakitani omwe akukhudzidwa ndi kupanga enzyme yothandizira kuchiritsa.

Mapulotesikidwe ena ndi zomwe zimachitika

Kuyerekeza ndi Aspn ndi Gluc

ASPN ndi Gluc Denter acidic Amino Repords, amapereka zambiri zowonjezera pazomwe zimaperekedwa ndi trypsin. Mapulotesiwa amapanga zosintha za pa Toptote zosinthika chifukwa cha zojambula zojambula zamakendi. Komabe, kutanthauza kwawo kwakukulu nthawi zambiri kumapangitsa kuti kuchepetsedwa kwa mapuloteni poyerekeza ndi trypsin.

Chymotryprin ndi mapurote

Chymotryprin amasiyana kuchokera ku trypsin pofunafuna zotsalira zazikulu za hydrophobic, monga tryptophan ndi tyrosine. Pomwe ili ndi ntchito yothandiza kwambiri, imagwiritsidwa ntchito nthawi zambiri pamapulogalamu ena. Broad - Ma proteses mwachindunji ngati proteinase k amapanga zosintha zambiri za pa Toptote

Maluso obwereza zingwe ndi trypsin

Kuzindikira mapuloteni

Chimbudzi chobwereza, chokhudza kugwiritsa ntchito trypsin kutsatiridwa ndi mapuloteni ena, kumapangitsa chizindikiritso cha mapuloteni. Mwachitsanzo, kudzudzula ndi trypsin kumatha kuwonjezera zidziwitso za mapuloteni a protenase k pofika 731%. Kwa magwiridwe antchito am'mandende, izi zimapangitsa kuti pakhale kubisala bwino, koyenera kuti mumvetsetse mitundu.

Kutetezedwa ndi ma peptides m'matumbo

Makalasi ang'onoang'ono omwe amapangidwa ndi trypsin amatetezedwa ku chimbudzi china mwanjira ina, ndikutsogolera ku zovuta kuposa kuneneratu siyico. Njira iyi imapindulitsa ogulitsa omwe amapereka ntchito zodzikongoletsera, chifukwa zimatsimikizira kuti ndizolondola pakudziwika ndi mawonekedwe a protein.

Kutanthauzira kwa ma fumotabola

Mapuloteke ndi mawonekedwe apamwamba, monga trsin, amakondedwa chifukwa cha zidutswa zomwe zimawonetseratu za peptive. Mosiyana ndi zimenezo, matebongo amakhala ndi zosakanikirana zovuta, kutanthauzira kwa data. Mafakitale omwe amatulutsa michere ayenera kuganizira kusamvana kumeneku kukwaniritsa zofuna za proteganic mosiyanasiyana.

Udindo wa Zymogins pama protease kuyambitsa

Proteaser

Mapuloteke nthawi zambiri amapangidwa ngati osagwira ntchito kuti asawonongeke mapuloteni am'madzi. Trypsinogen, adayambitsa matumbo ang'onoang'ono, akuwonetsa bwino lingaliro ili. Kuzindikira kubwereza kwa Zymogn ndikofunikira kuti mafakitale akupanga mapuloteni othandizira amagwiritsa ntchito, kuonetsetsa kuti ma enzyme amaperekedwa m'malo olamulidwa.

Malamulo a Entenokinase

Endokinase amapeza gawo lofunikira pakuyambitsa trypsinogen, kulimbikitsa kufunikira kwa lamulo lolondola pamakonzedwe a protease. Kuwongolera pakati pa zymogn kaphatikizidwe ndi kutsegula kumatsimikizira kuti proteric ntchito imapezeka bwino mkati mwa thupi, chinthu chomwe chimapangitsa kuti othandizira atulutsidwe mu ma syzyme.

Ma protease inhibitors ndi ntchito zawo zachilengedwe

Kuwongolera kwa protengotic

Mapulote zoletsa ndizofunikira pakuwongolera ntchito proterolytic machitidwe. Amapewa kuwonongeka kosalamulirika kwa mapuloteni, njira yofunikira kwambiri m'thupi apamwamba. Mu cell mankhwala, kumvetsetsa bwino kuli kofunikira pakupanga chithandizo chomwe chimagwira ntchito purces moyenera.

Protease zoletsa mu achire

Ma protease inhibitors sikuti ndi maudindo mumitundu yathanzi komanso m'malonda othandizira. Amapereka mapindu omwe angakhale, monga kuteteza impso kuchokera ku maantibayotiki kapena kuchepetsa chiopsezo, kuwonetsa kufunikira kwawo pakufufuza zamankhwala ndikukula.

Ntchito za mafakitale ndi biotechnological za trypsin

Udindo Wa Biotechnology

Trypsin imagwiritsidwa ntchito kwambiri mwanjira zosasinthika, monga chimbudzi cha protein ndi kusiyanitsa kwa foni. Kutengera kwake komanso kuchita bwino kumapangitsa kuti ikhale chisankho chokonda kwambiri, kuphatikiza m'mafakitale omwe amatulutsa mankhwala a foni, komwe kusinthika kwa enzymatic ndikofunikira.

BlueKKit Njira

Bluekit imapereka mayankho okwanira kuthana ndi mapuloteni ofunikira a kafukufuku ndi mafakitale. Mwa kupereka - ogulitsa kwambiri komanso mapuloteni ena, buluu amawonetsetsa kuti maloborator ndi mafakitale ali ndi zida zofunika kuti apititse patsogolo ntchito zawo ku proteomic, mankhwala othandizira, komanso biotechchnology. Kudzipereka kwathu kumayiko ena kumatipatsa mwayi wotsogolera, kukuthandizani kukwaniritsa zabwino komanso zotsatira zabwino pazomwe mumachita pasayansi.