Kodi Kuyesa Kotsalira kwa DNA ndi chiyani?

Kuzindikira Kuyesa Kotsalira DNA

Kuyamba Kuyesa Kotsalira DNA

Kuyesa kotsalira kwa DNA kumatanthauza njira zowunikira zomwe zimagwiritsidwa ntchito ndikupanga kuchuluka kwa DNA yomwe imakhalabe mu biopharmaical zinthu zopanga njira. Kuyesedwa kwamtunduwu ndikofunikira pakuwonetsetsa kuti pakhale chitetezo ndi chiyero cha biologics, kuphatikizapo kuchiritsa kwa ma cell, katemera, ndi ma antibodies. Kukhalapo kwa otsalira ku biopharaumicals, makamaka DNA kuchokera ku maselo onyamula anthu ngati E.Coli, kumabweretsa zoopsa kuphatikizapo immunogenicity ndi chotupa. Chifukwa chake, kuyesa kokhazikika kwa DNA ndi gawo lofunikira kwambiri pakupanga kwapamwamba ku biopharmaceutical.

● Tanthauzo

Kuyesa kotsalira kwa DNA kumaphatikizapo kupezeka kwa zidutswa ndi kuchuluka kwa zidutswa za DNA zomwe zatsalira kuchokera ku maselo omwe amagwiritsidwa ntchito popanga mabilogalamu. Zidutswa izi zimatha kukhala zosiyanasiyana kukula komanso kuchuluka, komanso kuchuluka kwa miniti kumatha kukhala kofunika. Kufunika kwa Kuyesa kotsalira kwa DNA sikungakhale kochulukirapo - kumatsimikizira kuti biopharauticals amakumananso ndi mfundo zowongolera za chitetezo ndikutha, motero kuteteza thanzi.

● Gwiritsani ntchito mphamvu

Kuwongolera kwapadera ku biophacruutical kumaphatikizapo magawo angapo, kuchokera kutsimikiziro zamitundu yambiri ku mayeso omaliza. Kuyesa kotsalira kwa DNA ndi gawo lofunikira mu mawonekedwe awa. Zimatsimikizira kuti njira zoyeretsa zatsukidwa bwino zathetsa zinthu zomwe sizingachitike, zimawonetsetsa kuti zitsimikizizi zomaliza za chitetezo zimakhazikitsidwa ndi madongosolo otetezedwa monga olamulira ngati FDA ndi EMA.

Udindo wotsalira DNA ku Biopharmaceuticals

● Mitundu ya biopharmaceuticals

BiopharMalumicals imaphatikizapo zinthu zosiyanasiyana, kuphatikiza ma antibododies osiyanasiyana, mapulateni obwezera, makatemera, ndi ma cell. Gulu lirilonse limakhala ndi njira zapadera zopangira, komabe onse amatengekedwa ndi kuipitsidwa kotsalira kwa DNA.

● Magwero otsalira DNA

DNA yotsalira imachokera ku maselo omwe amagwiritsidwa ntchito popanga. Maselo wamba omwe amaphatikiza maselo a bakiteriya monga E.coli, maselo a yisiti, maselo a nyama, ndi ma cell. Pakapangidwe a faifarafiticals, maselo awa amakankhira kukolola zomwe mukufuna, zomwe zitha kumasula zinthu zawo mwa kusakaniza.

Mfundo za TOAQman Probe mu DNA

● makina ochita

Taqman Probe - Ganizeni ndi njira yogwiritsidwa ntchito kwambiri yopezeka kotsalira. Njira iyi imagwiritsa ntchito vebe la flureorestenty omwe hybritchirazizs amatanthauza kuti ndi chidwi chenicheni. The Taq Polymerase adawululira enzyme panthawi ya PCR Kukweza kwa PCR, kulekanitsa utoto wa flueoreschesi kuchokera ku Quencher ndikupanga chizindikiro.

● Ubwino wa Taqman Probe

Chimodzi mwazabwino kwambiri za Taqman Probe ndizachipembedzo. Kutha kwa mfundo za Probe kuti mufotokozere mwatsatanetsatane kumatsimikizira kuti DNA yandamale yokha ndi yomwe yakwezedwa yokha ndikukukwezedwa, imachepetsa, kuchepetsa zabwino zabodza. Njira iyi imathandiziranso chidwi chachikulu, ndikupanga kukhala chabwino pakuwona kotsika kotsalira kwa DNA yotsalira.

E.Coli ngati khungu lanyumba ku biopharmaceuticals

● Chifukwa chiyani E.Coli amagwiritsidwa ntchito kawirikawiri

E.coli ndi selo lokonda kwambiri ku biotechnology chifukwa chakukula kwake mwachangu, chabwino - ma genetics odziwika, komanso kuthekera kofotokozera ma proteins apamwamba. Makhalidwe awa amapanga e.coli mtengo - Kusankha bwino komanso moyenera - kupanga.

● Zotsatira zotsalira e.coli DNA

Ngakhale zili zabwino zake, kugwiritsa ntchito E.Coli kumabwera ndi chiopsezo cha kuipitsidwa kotsalira kwa DNA. DNA yotsalira iyi imatha kuyambitsa mavuto, monga kuthekera kwa gene yopingasa kapena kukhalapo kwa endotoxins. Chifukwa chake, njira zotsalira za DNA zoyeserera ndizofunikira mukamagwiritsa ntchito E.Coli ngati mwininyumba.

Njira Zodziwikiratu

● Maluso omwe amagwiritsidwa ntchito mu kuchuluka

Njira zingapo zimagwiritsidwa ntchito pofufuza zotsalira za DNA, kuphatikiza qpcr, digito pcr, ndi chotsatira - mbadwo. Njira iliyonse imapereka zabwino zosiyana pankhani ya kuzindikira, mwachindunji, ndi kutulutsa.

● Kuzindikira ndi kulondola

Kuyesa kotsalira kwa DNA, chidwi ndi kulondola. Njira monga QPCR ndi Digital PCR ikhoza kuzindikira DNA pamiyeso ya femetogram, kupereka chidwi chachikulu chofunikira kuteteza chitetezo chambiri. Kulondola ndikofunikanso, chifukwa pamawonetsere kudalirika kwa zotsatirapo zake, kulola kusankha kwa chidaliro - Kupanga Kuwongolera Kwapadera.

Kukula kwa FG Weenection

● Kutanthauzira kwa FG

Gawo la FG likunena za ma femetogragram, gawo la muyeso woimira 10 ^ - magalamu. Kuzindikira DNA pa mulingo wa femitogram kumawonetsa chidwi chovuta kwambiri chodziwika bwino chodziwitsa kuchuluka kwa majini.

● Kufunika kwa chidwi chachikulu

Chidwi chachikulu pakuyesa kotsalira kwa DNA ndikofunikira pakuwonetsetsa chitetezo cha biopharmaceuticals. Kuzindikira DNA pagawo la FG kulola kuti zizindikiritse ngakhale zodetsa zazing'onoting'ono kwambiri, onetsetsani kuti chinthu chomaliza ndi chokwanira komanso chimakwaniritsa miyezo yolimba.

Njira Zowongolera Zowongolera mu biopharmaceutical

● Kufunika kotsalira kwa DNA

Kufunika koyesedwa kotsalira kwa DNA Kupanga kosiyanasiyana kumayambira pangozi yomwe ingachitike ndi kuipitsidwa kwa genetic. Mabungwe owongolera amalamulira malire a DNA yotsalira ya DNA, njira zokwanira zoyeserera kuti zitsimikizire kuti zikugwirizana.

● Miyezo Yoyang'anira

Miyezo yowongolera yotsalira yotsalira yotsalira kutengera mtundu wa biophormaceutical. Mwachitsanzo, FDA ndi EMA wakhazikitsa malangizo omwe amatchulanso malire otsalira otsalira a DNA mu zinthu zosiyanasiyana. Kutsatira miyezo imeneyi ndikofunikira pakuvomerezedwa ndi msika.

Ntchito mu mapuloni obwezera mapuloteni

● Kafukufuku wapadera

Zoyeserera zoyambirira za Protein, kuyesedwa kotsalira ndikofunikira pakuwonetsetsa kuti zopangidwa ndi zinthu. Kafukufuku wapadera akuwonetsa kugwiritsa ntchito bwinoE.Coli DNA Refedierial GetS kuwunikira ndi kuwongolera kwa DNA kuwonongeka kwa DNA, onetsetsani kuti akumvera malamulo oyang'anira.

● Chitsimikizo Chachilengedwe

Chitsimikizo Chachikhalidwe cha Produnmin chopanga protery chimaphatikizapo zigawo zingapo zoyesedwa ndi kutsimikizika. Kuyesa kotsalira kwa DNA kumagwira ntchito yofunika kwambiri pankhaniyi, kupereka tsatanetsatane kuti mutsimikizire kuti njira zoyeretsa zathetsa mavuto amtunduwu.

Zovuta mu Kuyesa Kotsalira DNA

● Mavuto aukadaulo

Chimodzi mwazovuta zoyeserera zotsalira za DNA ndi zovuta za kuwunika ndikupanga ma DNA otsika. Zinthu monga zitsanzo za matrix, kuphatikizika kwa DNNA, ndipo kusokoneza kungalepheretse kuyesa.

● Kugonjetsa zopinga zambiri

Kuthana ndi zopinga izi kumafuna kugwiritsa ntchito njira zapamwamba komanso ma protocol okwanira. Opanga ndi othandizira a E.Coli DNA amagwira ntchito mosalekeza kuti agwire ntchito zopanga zawo, kukulitsa chidwi ndi kusanjana ndi zofuna za mafakitale.

Zochita zamtsogolo zotsalira za DNA

● Kupita patsogolo

Kupita patsogolo kwaukadaulo kumadetsedwa kuti athe kuyesedwa kotsalira kwa DNA. Zojambula monga Crispr - Chiwonetsero chazotengera, digito PCR, komanso njira yotsatira -

● Njira ndi zida ndi zida

Njira zakutha ndi zida zomwe zimatsalira pakulonjeza za DNA zomwe zimapangitsa kuti zikhale zolondola komanso njira yothandizira kukonza. Kupita patsogolo kumeneku kudzathandiza opanga malo kuti akwaniritse mfundo zogwirizana kwambiri poyambitsa chitetezo chambiri.

Mapeto

Kuyesa kotsalira kwa DNA ndi gawo lovuta kwambiri pazinthu zabwino kwambiri pakupanga kwa biophormaceumical. Kuzindikira ndi kuchuluka kwa DNA kumakhala kofunikira pakuwonetsetsa kuti ndi mabizinesi. Njira Zapamwamba Monga momwe Taqman Probe imapereka chidwi komanso kusandulika pamafunika kutsatira mfundo zowongolera. Makampani akamapitirirabe, zotulutsa m'mayeso a DNA zomwe zimapangitsa kuti zikhale zofunika kwambiri pakusunga zabwino kwambiri za malonda.

● PafupifupiBuluuni

Jiangsu Hillgene, pansi pa dzina la Blank Blank, adakhazikitsa likulu lake ku Suzhou wokhala ndi mbewu 10,000 gm ndi malo a R & D. Ndi masamba opangira ku Shenzhen, Shanghai, ndi tsamba latsopano lomwe likumangidwa ku North Carolina, mapiri akukulitsa kukhalapo kwawo padziko lonse lapansi. Kampani imagwira ntchito yopanga chitukuko cham'madzi cha mankhwala a cell, othandizira pa chitukuko chagalimoto - t, TCR - Th cell netlet. Bluekit yadzipereka popititsa patsogolo luso lothandizirana ndi kuperekera mankhwala a cell. What is residual DNA testing?