Mafala Akutoma AL IL - Makasitomala A 2
● Tanthauzo
Interleukin - 2 (Il - 2) ndi ma cytokine a pathupi amthupi mwanu, makamaka omwe amatenga nawo gawo la maselo oyera, omwe ndi ofunika poyankha thupi. Anthu a IL - Kanema wa 2 ndi zida zazikulu zomwe zimagwiritsidwa ntchito mu kafukufuku komanso makonda azachipatala kuti ayezere ndende ya Il - 2 zitsanzo zosiyanasiyana zachilengedwe. Izi zikuluzikulu ndizomwe zikuwonetsa kumvetsetsa kwathu kwa kuyamikira kwa chitetezo chamthupi komanso chifukwa cha chithandizo chatsopano, kulimbikitsa kufunikira kwa mapulogalamu a cell.
Kufunika kwa IL - Kuzindikira 2 kumapitilira magawo angapo, kuphatikiza summunology ndi ku Ofculogy, pomwe kumvetsetsa kusinthasintha kwa kusintha kwa mayankho amthupi kumabweretsa kukhazikika pakuthandizira pakumwanitsa. Monga makampani azomwe amapezeka, kufunikira kwachidziwikire, komanso kukhala odalirika kwa IL - Makambo odziwika bwino, makalata amayendetsa kuchokera kwa opanga ndi othandizira padziko lonse lapansi.
● Mitundu ya zitsanzo za zitsanzo zomwe zimagwiritsidwa ntchito
Anthu a IL - Zingwe 2S itha kugwiritsidwa ntchito pazitsanzo zosiyanasiyana zachilengedwe, kuphatikiza seramu, magunkhu, ma elkma, zikhalidwe zazikulu zapa foni, ndi magazi athunthu. Mtundu uliwonse wa zitsanzo zimaperekanso chidziwitso chapadera komanso zovuta zomwe zimapangitsa kuti kupezeka, zomwe zimapangitsa kuti zitheke zidalike kwambiri. Mwachitsanzo, kupenda zikhalidwe zachikhalidwe zilibe kanthu kumatha kupereka tsatanetsatane wa zopanga cytokine ku Vitro, zomwe ndizofunikira kwambiri pamaphunziro a ISATSOPANO.
Makina a Il - Kuzindikira 2
● Ntchito ya ma antibodies ndi ma enzyme
Chovala cha IL - Khoma 2 limadalira kugwiritsa ntchito ma antibodies ndi michere kuti adziwe makamaka il - mamolekyu awiri. Nthawi zambiri, ma kits awa amagwiritsa ntchito sangweji ya Elisa (enzyme - Chimodzimodzi mwa immunorbentry). Munjira iyi, Il - Ponena za 2 mu zitsanzo zagwidwa pakati pa zigawo ziwiri za ma antibodies awiri: Onti-anti ogwidwa womangidwa ndi enzyme yolumikizidwa ndi enzyme. Enzyme imakumana ndi gawo lapansi kuti itulutse kusintha kwa utotometric komwe kumatha kuyesedwa moyenerera.
Zomverera za njirayi komanso mwachindunji zimadalira kwambiri ma antibodies omwe amagwiritsidwa ntchito, ndichifukwa chake kusankha munthu woyenera wa IL - Chovala cha 2 chodikira kapena wopanga ndi wotsutsa. Opanga odziwika bwino omwe ma antibodies amayesedwa mwamphamvu komanso kutsimikizika kuti apereke zotsatira zabwino.
● Kukula kwa utoto ndi Il - kuchuluka kwa 2
Pangano la kuchuluka il - magawo awiri mu zitsanzo pogwiritsa ntchito kukongoletsa ndi mtundu wa utoto wopangidwa ndi enzyme - Kusintha kumeneku ndikufanana mwachindunji ndi kuchuluka kwa il - 2 mu chitsanzo. Mwa kuyeza zotengeka pamlingo wina, ofufuzawo amatha kudziwa bwino Il - 2 ndende pogwiritsa ntchito chokhazikika chomwe chimapangidwa kuchokera kuzomwe zimadziwika.
Mapulogalamu a IL - Kuzindikira 2
● T
- 2 ili ndi gawo lofunikira pakukula ndi kuchuluka kwa ma cell a T, zomwe ndizofunikira kuti zipikisane. Anthu a IL - Makadi awiri amazindikira kuti afufuze mayankho amtundu wa T, kupereka chidziwitso mu malamulo a chitetezo cha myuni. Izi zili ndi tanthauzo osati kafukufuku wokha komanso chifukwa chopanga chithandizo chamankhwala chopingasa cha Vacuri - Mavuto ena.
● Khansa ya Immunumy komanso chitetezo
Udindo wa IL - 2 Polimbikitsa mayankho a mthupi amamangiriridwa ku khansa yathupi immunotherapy, makamaka pakuchiza yomwe imafuna kukulitsa mphamvu ya chitetezo chathupi yomenyera zotupa. Anthu a IL - Makambo a 2 ndiofunika mu makonda awa, kulola kuwunika kwa chitetezo chamthupi komanso mayankho othandizira, potero akuthandizira kutsanzira kwa protocols.
Kuchulukitsa kwa Il - Mitundu Yakale 2
● EliSPot Kits: chidwi ndi njira
Elispot (enzyme - Chimodzimodzi kammumbospot) Malingaliro amadziwika kuti akumva zowoneka bwino, omwe amatha kupeza cytokines pa imodzi - Maselo. Anthu a IL - 2 Elispot Kits ndizothandiza kwambiri pozindikiritsa Il - 2 maselo obisika mkati mwa anthu, amapezeratu ntchito ndi mayankho oyenerera a mayankho amthupi. Kukhudzizana kwakukulu kumeneku ndi kofunika kwambiri pakufufuza kwa zinthu wamba, makamaka mukazindikira - Mayankho a Cytokine mu makonda osiyanasiyana.
● Elisa Kits: Njira zokwanira
Elisa Kits ndiosavuta kupezeka kwa cytokine, kupereka njira yolimba komanso yowongoka yokwanira il - 2 m'magawo osiyanasiyana. Anthu 2 Il - 2 Elisa Kits amasankhidwa chifukwa chodalirika komanso mosavuta kugwiritsa ntchito, kuwapangitsa kukhala osasunthika m'malesities kupeza phindu, katemera wake, komanso matenda a matendagenesi.
Mawonekedwe a Elispot
● Microphete - zokambirana
Mtundu wa microphete ya EliSPot Intays amalola okwera - Kusanthula kwa Yesu, komwe ndi kopindulitsa mu maphunziro akulu. Kutha kuyesera zitsanzo zingapo nthawi imodzi kumathandizira kulimba kokha ndikuchepetsa kusiyanasiyana, kupereka zambiri zodalirika pazoyeserera zingapo.
● Kuzindikira kwambiri kwa mayankho otsika
Chimodzi mwazinthu zoyimilira za Elispot kits ndi kuthekera kwawo kozindikira mayankho a maselo, omwe ndi ovuta pakufufuza komwe - zochitika za pafupipafupi Kumverera kumeneku kumapangitsa Esispote chida chabwino kwambiri cha kafukufuku wa katemera ndi chitukuko cha kufalikira kwatsopano.
Elisa Kit Njira yofotokozedwera
● Sandwich Elisa Maluso
Sangweji Elisa ndiye njira yofunikira yogwiritsidwa ntchito mu ul Il - matchulidwe a 2 chifukwa cha tanthauzo lake komanso chidwi chake. Njirayi imaphatikizapo koyamba wa IL - 2 kwa antibody, yotsatiridwa ndi enzyme yachiwiri -
● Kuyeza IL - 2 mu plasma ndi maulamuliro
Anthu 2 Il - 2 Elisa Kits amagwiritsidwa ntchito kwambiri kuyeza milingo ya cytokine mu zikhalidwe zama plasma ndi foni. Kutha Kusanthula IL - 2 Kutenga Madzimadzi amtunduwu ndikofunikira kuti mumvetsetse zantchito komanso mayankho amthupi, makamaka pakufufuza zomwe zimayang'ana kwambiri komanso njira zochizira.
Tsimikizikilo wa Elisa
● Gawo - ndi - Njira
Njira yodziwika ya atongumetric Elisa imakhudzana ndi njira zingapo zoyambira ndi kukonzekera kwa zitsanzo, kutsatiridwa ndi makulidwe ndi ma antibodies. Pambuyo pamwazi zingapo kuti muchotse zinthu zosawoneka, mankhwala opha chizindikiritso amawonjezeredwa. Gawo lomaliza limaphatikizapo kuwonjezera gawo lomwe limachita ndi enzyme kuti ipangitse kusintha kwapakati, kupereka zambiri pa IL - magawo awiri.
● Kuchuluka kwa chikhalidwe cha khungu
Kuchulukitsa il - 2 mu cell exines alentel amapereka chidziwitso mu ma cytokine kupanga zinthu zoyeserera. Izi ndizofunikira kwambiri mu maphunziro ofufuza za mayankho amthupi, pomwe kudziwa kuchuluka kwa ma cytokine kungathandize kumvetsetsa njira zamankhwala komanso zotsatira za mankhwala osokoneza bongo.
Kufunika Kwa Wel Il - Kukula kwa 2
● Kufufuza komanso zovuta zamankhwala
Olondola Il Ill - Muyeso 2 ndi maziko kuti mufufuze komanso kugwiritsa ntchito mapulogalamu azachipatala. Pofufuza, zimathandizira kuti anthu mayankho a mthupi athe, kudziwitsa chitukuko cha immunotherapries ndi katemera. Zachipatala, Il - magawo 2 amatha kukhala ngati biomarkers pamatenda kapena othandizira kuyankha, othandizira oyang'anira odwala komanso kukhathamiritsa.
● Zokhudza Phunziro la Cytokine ndi mankhwala
Kutha kuyeretsa Il - 2 molondola ali ndi malingaliro akulu a phunziro la cytokine ndi chithandizo chamankhwala. Imathandizira kumvetsetsa mwakuzama kwa ma netiweki a cytokine ndi maudindo awo mu thanzi ndi matenda awo, kumathandizira chitukuko cha njira zochizira za cytokine zomwe zitha kusintha chitetezo cha mungu.
Zovuta ku Il - Kuzindikira 2
● Maganizo ndi zochitika zina
Chimodzi mwazovuta zoyambirira ku IL - Kuzindikira 2 kuwonetsetsa kuti ndi chidwi. Kusintha kwa magwiridwe antchito kumatha kubweretsa kusagwirizana, kutsimikizira kufunika kosankha munthu wodalirika wa IL - Zida Zapamwamba Zapatali Zopanga ndi Ogulitsa omwe akupanga mtundu wa zinthu zawo.
● Kusintha kwa zitsanzo
Vuto linanso lalikulu ndi kusintha komwe kumayambitsidwa nthawi ya zitsanzo ndikukonzekera, zomwe zingakhudze kulondola ndi kubereka kwa zotsatirapo. Protocol yokhazikika ndi maphunziro ndiyofunikira kuti muchepetse izi, ndikuwonetsetsa kuti deta yomwe yapezeka kuchokera kwa munthu - Kuzindikira kwa 2 ndi kudalirika.
Zoyembekeza Zamtsogolo ku Il - Zindikirani
● Kupititsa patsogolo ntchito
Tsogolo la anthu IL - Kits 2 likuwalonjeza, ndi kupita patsogolo kwa ukadaulo kumalimbikitsa chidwi chawo, mwachindunji, komanso kugwiritsidwa ntchito mosavuta. Zopanda mawonekedwe monga ma mealx ndi point - a - Kuyesedwa mosamala kumatha kukulitsa mapulogalamu awa, kuwapangitsa kukhala opezeka bwino komanso ogwira ntchito zosiyanasiyana.
● Ntchito zokulirapo
Kumvetsetsa kwathu kwa Il - biology 2 kukukulira, momwemonso mapulogalamu a IL - Chikhumbo cha 2. Mapulogalamu achitetezo a m'mafano a IL - 2 Njira zili pafupi, zomwe zingathetse njira zothandizira chithandizo chamankhwala cha autoimmune matenda, khansa, ndi kupitirira. Kufatsa Kwambiri kwa IL - Zingwe 2 zopanga zopanga ndi othandizira zikhala zofunikira kwambiri pakukumana ndi zosowa za kutuluka.
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Post Nthawi: 2024 - 12 - 09 15:26:03
