Chiyambi
Kuchotsa kwa DNA ndi mtundu wa maziko mu subilogy biology, kusewera gawo lofunikira mu kafukufuku komanso kugwiritsa ntchito zamankhwala. Kukula kwaGenomiki DNAs yasintha izi, ndikupangitsa kuti ikhale, yothandiza komanso yodalirika. Nkhaniyi imakhudzanso kutsuka kwa genimic DNA, Kuyang'ana mitundu yoyenga, njira zoyenga, zokumana nazo, zoyeserera zapamwamba, mapangidwe am'malire, komanso zomwe zimachitika mtsogolo. Kutsindika mwapadera kumayikidwa pa kufunika kwa cell mankhwala a genetiam DNA. Kaya ndinu ofufuza, Docticiaty, kapena labotale ya labotale, yotsogolerayi imapereka chidziwitso chofunikira m'masita ndi kupita patsogolo m'malo mwa genimic dna.
Mafala Akutoma Nawo DNA
● Mwachidule za kuyeretsedwa kwa DNA
Chuma cha DNA CNA chipadera ndi zida zofunikira mu labootor labolotories. Ma Tots awa amakhumudwitsa njira yochotsera, ndikuonetsetsa kuti - DNA yabwino kwambiri imapezeka m'malo osiyanasiyana. Amapereka purotocol woyenera, kuchepetsa kusinthasintha ndikusintha kubereka. Kupezeka mu mitundu ingapo, anthuwa amathandizira mitundu yosiyanasiyana yosiyanasiyana ndi zofunikira zakumwa.
● Kufunika kosiyanasiyana
Kuyeretsa kwa DNA Kits ndikofunikira pakufufuza kwa ma genetic, matenda azachipatala, zolosera, komanso maphunziro azachilengedwe. Pofufuza, amathandizira kuphunzira za majini, mitundu, ndi kusintha kwa Eganetic. Muzachipatala, amagwiritsidwa ntchito pozindikira matenda a genetic, kuwunika chithandizo chamankhwala, ndikupanga mankhwala. Kudalirika komanso kuchita bwino kwa ma kits awa ndi paramount pakupanga zotsatira zoyipa ndi zoletsedwa.
Mitundu ya zitsanzo za DNA kudzipatula
● Maselo oyera
Genimic DNA imatha kusinthidwa moyenera kuchokera m'maselo oyera pogwiritsa ntchito ma kilogalamu apadera. Izi ndizothandiza kwambiri mu matenda azachipatala ndi kuyezetsa majini, komwe zitsanzo za magazi zimasonkhanitsidwa.
● minofu minofu maselo
Ma cell amisisi chikhalidwe amapereka malo olamulidwa kuti aphunzire zinthu zamtundu. Kuchotsa kwa DNA kuchokera ku maselo awa ndikofunikira pakufufuza kwa sayansi yam'manja ndi maselo amisala. Makilogalamu omwe adapangira minofu azitsulo maselo amaonetsetsa kuti zokolola ndi zoyera.
● nyama ndi zobzala ziwalo
Kutulutsa DNA kuchokera ku nyama ndi zobzala zimakhala zovuta zapadera chifukwa cha kupezeka kwa inhibitors ndi ma cell a cell. Makampani apadera a DNA amapangidwa kuti azitha kuthana ndi zovutazi, ndikuonetsetsa za lysisice ndi kuyeretsa.
→ yisiti ndi mabakiteriya
Yisiti ndi bakiteriya maselo akuluakulu a cell omwe amafunikira njira zapadera za nyimbo zothandizira DNA. Ma Kits omwe amaphatikizidwa ndi tizilombo toyambitsa matendawa amagwiritsa ntchito ma enzymatic njira zosokoneza bongo kuti amasule - DNA ya General Gene.
Gawo - ndi - PROTER DNE
● Kukonzekera koyamba
Gawo loyamba mu kuyeretsa DNA kumaphatikizapo kusanja ndikukonzekera zitsanzo. Izi zingaphatikizepo kuphatikiza centrifugation, kutsuka, ndi homogenization, kutengera mtundu wa zitsanzo. Kukonzekera kwa zitsanzo zoyenera ndikofunikira kuti muwonjezere zokolola za DNA ndi mtundu.
● Lysis ndi magawo omangamanga
Gawo la Lysis limaphatikizapo kuponyera ma cell nembanemba ndikumasulira ma genimic DNA mu yankho. Izi zimatheka pogwiritsa ntchito zotchinga, ma enzymes, kapena njira zamakina. Kuphatikizika kumatsata, komwe DNA imamangidwa ndi gawo lolimba, monga silika, pansi pa mchere wamtundu wambiri.
● Kutsuka ndi kuchita zinthu zolakwika
Njira zotsukira zimachotsa zodetsa ndi zodetsa, kuonetsetsa kuyera kwa DNA. Zovala zingapo zotsukira zimagwiritsidwa ntchito kuthana ndi mapuloteni, lipids, ndi zinyalala zina. Pomaliza, gawo lazolowere limatulutsa DNA yoyeretsedwa ndi gawo lolimba pogwiritsa ntchito buffer kapena madzi otsika.
Njira Yothetsera - Njira Zadziko Lonse Kudzipatula
● Kuyerekeza ndi njira zina
Njira Yothetsera - Njira Zazikidwa, monga phenorol - chlorotorm - chokoromor - chokoromorm kuchotsera ndi mchere, ndi njira zachikhalidwe cha DNA POPANDA. Ngakhale ali ogwira mtima, amagwira ntchito - Olimba ndipo amafunikira mankhwala owopsa. Mosiyana ndi izi, makono amakono a DNA amapereka zotetezeka, mwachangu, komanso ogwiritsa ntchito - njira zina zokondweretsa.
● Ubwino wa yankho - Njira
Njira Yothetsera - Kutengera Kutulutsa Ma Kits kumapereka maubwino angapo, kuphatikizapo zokolola zapamwamba, zoyera, komanso kubereka. Adapangidwa kuti achepetse kuipitsidwa ndipo amatha kukwezedwa mosavuta - Kugwiritsa Ntchito Kutulutsa. Komanso, safuna mankhwala owopsa, kuwapangitsa kukhala otetezeka kwa ogwira ntchito a labotale.
Zovuta mu Chiyeretso cha DNA
● Nkhani zofala zomwe zakumana nazo
Kuyeretsa kwa DNA kumatha kukhala ndi zovuta, monga zokolola zochepa, kuipitsidwa, komanso kuwonongeka. Zilonda zomwe zilipo mu zitsanzo zimatha kusokoneza mafomudwe otsika, ndipo kusamalira molakwika kumatha kubweretsa DNA.
● Malangizo osokoneza bongo
Kuti tithe kuthana ndi mavutowa, ndikofunikira kutsatira protocol mokwanira. Kugwiritsa ntchito ma reagents atsopano, kukhalabe oyenera ntchito yoyera, ndikuwonetsetsa kuti pokonzekera zitsanzo mosamala kumathetsa mavuto ambiri. Kuphatikiza apo, kuphatikiza njira zowongolera zowongolera, monga kuyeza DNA Kulimbana ndi kuyera, zitha kuthandiza kuzindikira ndikuthana ndi mavuto.
Ntchito Zofufuza ndi Mankhwala
● Gwiritsani ntchito kafukufuku
Chuma cha DNA chimayambitsa kafukufuku wofufuza kwa majini, zomwe zimathandizira maphunziro ogwiritsa ntchito mtundu, malangizo, komanso kulumikizana. Imathandizira maluso ngati PCR, zotupa, komanso zowoneka bwino, zomwe ndizofunikira kuti timvetsetse ma genetic ndikupanga njira zatsopano zochizira.
● Mapulogalamu mu matenda azachipatala
Mu matenda azachipatala, okwera - DNA yanyimbo yabwino ndi yofunikira pakuzindikira matenda olondola matenda, Hzinosis, ndi kuwunikira mankhwala. Ntchito zofunsira zimaphatikizapo kuyezetsa ma genetic kwa matenda obadwa nawo, amuna okhaokha, komanso kupezeka kwa matenda opatsirana. Kudalirika kwa DNA kumayambitsa makomweko ndikofunikira popereka chidziwitso cholondola komanso chanthawi yake.
Khalidwe ndi zokolola zoyeretsedwa DNA
● Zinthu zokhudzana ndi DNA zabwino
Pali zinthu zingapo zomwe zimapangitsa mtundu wa DNA yoyeretsedwa, kuphatikizapo mtundu wake, njira yochotsera, ndi kuwongolera njira. Kukhalapo kwa zoletsa, monga mapuloteni ndi ma polysaccharides, kumatha kukhudza mapulogalamu ena.
● Njira zoyezera kuyera kwa DNA
Kuyesa kuyera ndi kukhazikika kwa DNA ndikofunikira powonetsetsa kuti kutsimikizika kwake pakugwiritsa ntchito. Njira zofala zimaphatikizapo kusanthula kwa mawonekedwe a Spectophotomomet, yomwe imapereka chiwerengero cha ophatikizira (A260 / A280) kuti athetse kuyera, komanso mafayilo a fluorometric, omwe amapereka magawo oganiza bwino komanso owoneka bwino.
Makina ndi kutsuka kwabwino kwa DNA
● Technologies yolimbikitsa njira
Kudziyeretsa mu DNA Kudziyeretsa kwakhala kofunikira kwambiri pakugwiritsa ntchito ma voliyumu akulu ndi kukonza ntchito. Makina Okhawo Okhazikika, monga ogwirira ntchito zamadzimadzi aboti ndi malo ogwirizira okhathamira, amaletsa cholakwika cha anthu, ndikuwonjezera zomwe zidatulutsa.
● Ubwino wa zothetsera zambiri
Njira zoyenga bwino za DNA zoyezera ndizofunikira kwambiri - maphunziro amtundu wa ma genetic, maphunziro azachipatala, ndi Biobaning. Amathandizira kukonza nthawi yomweyo ya zitsanzo zingapo, kusunga nthawi ndi zinthu zina. Makina Okhawo Amaperekanso zotsatira zosasinthasintha, kukhala wodalirika komanso kudalirika.
Kusanthula kwa Matamanja
● Kuyerekeza magwiridwe antchito otchuka
Pali mitundu yambiri ya negoni ya genimic DNA yomwe imaperekedwa kuchokera kwa opanga osiyanasiyana, aliyense wokhala ndi mawonekedwe ndi mawonekedwe. Zinthu zofunika kuziganizira mukamasankha zokolola, zoyera, zimangogwiritsa ntchito, ndi mtengo. Maphunziro ofananira ndi ndemanga za ogwiritsa ntchito amatha kupereka chidziwitso chofunikira mu ma kilogalamu osiyanasiyana.
● Ndemanga za ogula ndi akatswiri
Mayankho a ogwiritsa ntchito ndi akatswiri amasewera amatenga mbali yofunika kwambiri powunikiranso mphamvu ya ma genimic Draprection. Ndemanga zabwino ndi zivomerezero zodziwika bwino zimatha kuwongolera ofufuza posankha zida zabwino kwambiri pazosowa zawo. Kuphatikiza apo, mayankho ochokera kwa ogwiritsa ntchito amathandizira opanga kusintha zinthu zawo ndikuthana ndi zovuta zilizonse.
Zochita zamtsogolo mu maluso a DNA
● Matekinolojeni omwe akutuluka mu DNA Kutulutsa
Munda wa Kuyeretsa kwa DNA kumasintha mosalekeza, ndi matekinoloji atsopano omwe amatuluka kuti athe kuthana ndi zomwe sangathe. Zopanda mawonekedwe monga microfluidics, nanotechchnology, ndi maginito a maginito - Chovala cha Magnetic chikuyenda njira yopezera njira zoyenerera ndi zowongolera bwino.
● Kuneneratu zochitika zamtsogolo
Zochita zamtsogolo mu DNA chiyeretso cha DNA zitha kuyang'ana pa zoyendetsera nokha, miniaturization, komanso kuphatikiza ndi njira zina zamankhwala a sayansi. Kukula kwa ma kilogalamu wamba omwe amatha kuthana ndi mitundu yosiyanasiyana ndi mikhalidwe yomwe ikuyembekezeredwa kuti ipititse patsogolo kusinthasintha ndi kusintha kwa mapangidwe a DNA m'thupi.
Bluekit: Kuchita Upainiya DNA DZIKO LAPANSI
Jiangsu Hillgene, pansi pa dzinaBuluuni, adadzikhazikitsa ngati wosewera wotsogolera m'munda wa genimic DNA. Imeneyi ili ku Suzhou, malo opanga ku Shenzhen ndi Shanghai, ndi malo aku North Carolina, mapiri aku Norgene amapereka njira - a - Zogulitsa za buluu zimapangidwa kuti zikwaniritse zokambirana za ma cell mankhwala, kuthandizira kukula kwagalimoto - t, TCR - T, ndi Stem Cell. Ndi kudzipereka kwatsopano ndi mtundu, mapiri a mapiri amadzipatulira kuti apitirize kupita ku gawo la maselo othandizira ndi kukonza zotsatira za wodwala.
Mwachidule, kuyeretsa kwa genimic DNA ndikofunikira kwambiri pazinthu zamaselono, ndi mitundu yayikulu - ntchito yopuma pakufufuza ndi mankhwala. Kupita patsogolo kwa DNA Kutulutsa Mapako, kuphatikizaponso a Bluekit, zapangitsa izi kukhala zothandiza, zodalirika, komanso zopezeka. Pamene technology ikupitilirabe, tsogolo la DNA limakhala ndi mwayi wosangalatsa kwambiri wokulitsa zomwe zapezeka ndi sayansi komanso luso lazipatala.
Post Nthawi: 2024 - 09 - 27 14:40:06
