Chiyambi
● Kufunika kwa DNA
M'malo mwazomwe zimachitika pazinthu za genimic DNA ndi njira yofunika kwambiri yomwe imayikira maziko a magwiritsidwe a mapulogalamu, kuyambira kafukufuku ku matenda opatsirana ndi matenda omwe amapezeka komanso mankhwala. Njira ya genimic DNA imaphatikizapo kudzipatula ku ma cell kuti asanthule ndi kuwongolera ma genetic, ndikuwonetsa kumvetsetsa kwa genetic, za chisinthiko cha chisinthiko. Ndi chiwerengero chophatikizira cha maphunziro a genioc, udindo wodalirikaGenomiki DNAsanakhalepo pivotal. A Kits awa amalimbikitsa njira yodulitsira, ndikuwonetsetsa zokolola zambiri komanso zoyera za DNA, ndizofunikira pakugwiritsa ntchito.
Njira Zosakaniza
● Mitundu ya zitsanzo za DNA
Kukhulupirika kwa Genomic DNA kumayamba ndi zopereka - zitsanzo zabwino. Magawo wamba amaphatikizapo magazi, minofu, malovu, ndi ma swabs. Chitsanzo chilichonse chimapereka zabwino zapadera; Mwachitsanzo, zitsanzo za magazi zili zolemera mu DNA koma zimafunikira kusamalira mosamala, pomwe malovu ndi ma swabs amapereka ndalama zosakhala - Zosagwira. Kusankha chitsanzo choyenera ndikofunikira, makamaka pogwiritsa ntchito cemer drach drade, komwe kungakonzekere mitundu ya khungu, ndikuwonetsetsa zokolola zam'manja komanso zoyera.
● Zochita zabwino kwambiri zogwirizira
Kuonetsetsa kuti zitsanzo zikhale kukhulupirika ndikofunikira kuti zithandizire bwino DNA. Zitsanzo ziyenera kusungidwa mu kuipitsidwa - malo opanda ufulu, osungidwa pamatenthedwe oyenera, ndipo amathandizidwa ndi chisamaliro kuti ateteze zowonongeka. Njira iyi ndiyofunikira kuti mukuthana ndi zoyesayesa zatsopano kapena zomwe zimasungidwa kwanthawi yayitali, monga kuperewera konse kungasokoneze zokolola zokwanira za DNA ndi zabwino, zomwe zikukhudza zoyeserera.
Njira zam'madzi zam'madzi
● Mankhwala vs. Maluso a Lysisis
Gawo la cell lysis ndi chithunzithunzi potulutsa ma genionic drics kuchokera kuma cell. Chemical rissis, kuphatikiza zitsulo ndi ma enzyme, zimaphwanya pang'ono maselo ndi mapuloteni. Njira zakuthupi, monga kusokonezeka kwa makina ndi sonication - ndizovuta ndipo zitha kugwiritsidwa ntchito pa zitsanzo za Conterger. Kusankha njira nthawi zambiri kumadalira mtundu wa zitsanzo ndi zofunikira mwatsatanetsatane za mtundu wa jenemic DNA. Opanga amapangira ma kilogalamu kuti athetse ma lysis njira yoyendetsera bwino pakati pa kuphweka ndi kugwira ntchito bwino.
● Kufunika kwa lysis mu DNA
Nyimbo zoyenera zamadzi zimatsimikizira kuti zigawo zikuluzikulu zam'madzi zimawonongeka mokwanira, kutsogoleredwa kumasulidwa kwa DNA mu yankho. Kuphedwa moyenera lysis kuchepetsa kumeta kwa DNA ndi kuipitsidwa ndi mapuloteni ndi lipids, zomwe ndizofunikira makamaka m'mapulogalamu monga cell mankhwalawa ndi otsogolera.
Kuchotsa mapuloteni ndi zodetsa
● Udindo wa Kuyeretsa
Post - Fuysis, kuchotsedwa kwa mapuloteni ndi zodetsa zina ndizovuta. Ma sol sol sol sol - chloroform amagwiritsidwa ntchito mwamwambo wachikale ndi mapuloteni osiyana siyana kuchokera ku ma acid acid. Chuma chamakono chamakono chimagwiritsa ntchito sica - membranes kapena mikanda yamatsenga, yomwe imamanga kusankha mosankha mukamalola zodetsa kuti zisatsuke. Izi ndizofunikira kwambiri kuti mupeze DNA yoyenerera bwino komanso yokhazikika pamapulogalamu.
● Centriftigation adalongosola
Centrifuagation ndi gawo lodziwika lolekanitsa DNA kuchokera ku zosayera positi - Lysis. Pogwiritsa ntchito mphamvu ya centrifugal, zolemera zolemetsa ndi mapuloteni ndi mapuloteni omwe amapezeka, pomwe DNA idalipo kwambiri. Njira iyi imangobwerezedwanso mu mawonekedwe oyeretsa ndi kuyeretsa kokweza chiyero ndi kukhazikika kwa DNA.
Njira Yopanda Ntchito ya DNA
● Kugwiritsa ntchito isoppanol ndi ethanol
Kutengera mpweya ndi njira yapadera ya DNA kuchira, adagwiritsa ntchito pafupifupi mitundu yonse ya genetiamic. Mowa amakonda isopropanol ndi ethanol amathandizira DNA kupita ku DNA kupita kumtunda pochepetsa kusungunuka kwake. Pokhapokha pokonza, DNA imapanga zowoneka kapena zowoneka bwino, ndikupereka zowoneka bwino.
● Kuwona mafilimu a DNA
Kukhalapo kwa DNA Finals kumatsimikizira mpweya wabwino ndipo umakhala ngati chisonyezo choyambirira cha mankhwalawa. Izi, ngakhale zikuwoneka kuti zikuwoneka, ndizofunikira kuti mukhulupirire, kuonetsetsa kuti DNA yokwanira ipezeka pakuwunika kotsatira.
Kutsuka DNA
● Kufunika kwa kutsuka kwa ethanol
Kutsuka mapeleki a DNA ndi Ethanol kumatichotsa mchere komanso zosayera. Izi, ngakhale zikuwoneka kuti sizingachitike, ndizofunikira popewa kuipitsidwa komwe kumatha kukhudza njira zoyambira. Kutsuka kwa ethanol kumafotokozedwa moyenera mu buku lililonse la ogwiritsa ntchito ndi Genomic DNA Exregration Cida zowonetsetsa kuti akwaniritse zotsatira zabwino.
● Kukhazikitsa chiyero cha DNA
Kuyera ndikofunikira monga zokolola mu DNA m'zigawo. Zosakhazikika zimatha kuyika enzymatic zimachitika pakugwiritsa ntchito ngati PCR ndi sequening. Genomic DNAYERODYER RA Othandizira Ogulitsa amalimbikitsa chiyero, kupereka ma protocols ndi zida zomwe zimatsimikizira kuti ziwopsezo zimasambitsidwa bwino.
Kusungunuka ndikukonzekera DNA
● Kusankha buffer yoyenera
DNA ikayeretsedwa, imasungunuka mu buffer yoyenera, nthawi zambiri amafewetsa kapena madzi osungunuka. Kusankha kwa Buffer kumatha kusokoneza kukhazikika ndi kukhulupirika kwa DNA, kumapangitsa kuti ndikhale nthawi yayitali - kusiyiratu. Mafakitale a DNA DNAYERS amafalikira nthawi zambiri amapereka zobisika zokhudzana ndi mafomu ena.
● Kukonzekera DNA pakuyesa
Kukonzekera koyenera kwa DNA ndikofunikira kuti muchite bwino, onetsetsani kuti mukugwirizana ndi njira zowunikira monga Qpcr, Kenako - Mibadwo Yotsatira, ndi Kudula. Njira zokwanira zokonzekera bwino zimakulitsa kukhulupirika ndi magwiridwe antchito awa.
Kuwongolera kwabwino kwa DNA
● Njira zowunikira mtundu wa DNA
Pambuyo pochotsera, kuwunika mtundu wa DNA ndikofunikira. Spocseratomometry ndi njira yotchuka, kuyeza kuyanjana ndi 260 nm ndikupereka chidziwitso cha DNA kuzunzidwa ndi kuyera. Kuphatikiza apo, Geli Elsoctoresis imalola kuti aziona pa kukhulupirika kwa DNA, pofuna kunyozedwa.
● Kumvetsetsa kuwerenga kolota
Kuwerenga kowoneka bwino kumapereka chidziwitso pakukula kwa DNA. Chiwerengero cha 260/8/28 pafupi ndi 1.8 chikuwonetsa DNA, pomwe kupatuka kwapakati. Kuwerenga kumeneku ndi kofunikira pakuwonetsetsa kuti DNA ndiyoyenera pakugwiritsa ntchito.
Zovuta mu DNA Kuchotsa
● Nkhani zofananira komanso zovuta
Ngakhale kuti pali zibwenzi, zovuta mu DNA m'chokanizira chimapitilira, kuphatikizapo zokolola zochepa, kuipitsidwa, komanso kuwonongeka. Kumvetsetsa izi ndikutsatira malangizo ovuta, omwe nthawi zambiri amapereka opanga, ndikofunikira kuti muchotse bwino.
● Kusintha kwa mitundu yosiyanasiyana
Mitundu yosiyanasiyana imawonetsa zovuta zapadera mu DNA m'zigawo za DND Genomic DNA PERTOTERT Clower ogulitsa kuti athetse mitundu iyi, kupereka njira zogwiritsira ntchito zogwirizana pazitsanzo zosiyanasiyana zachilengedwe.
Kupita patsogolo kwa maluso a DNA
● Kutulutsa m'makombo
Kupita patsogolo kosalekeza ku Genomic DNeprection Kits asinthasintha njira, kukulitsa mphamvu, kuthamanga, ndi kudalirika. Izi zopangira zokhazokha zimaphatikizapo ukadaulo wa zamagetsi, zophatikizika zam'madzi, komanso kuphatikiza ndi mabotics kuti mugwiritse ntchito njira zapamwamba, ndikuwonetsa zofunikira zakufufuza kwa genion.
● Zochita zamtsogolo pa sayansi
Monga sayansi ya genomimim ikupita patsogolo, kufunikira kwa okwera - Kutulutsa kwabwino kwa DNA kumakula. Zochitika zamtsogolo zimaphatikizapo Eco - njira zochezera bwino, ndikuphatikizidwa kwabwino, ndi kuphatikiza kwa matekinoloje owunikira owunikira, ndikusintha njira yowunikira bwino kwambiri komanso zowonjezera.
BlueKIt: Upainiya mwa genomic ndi ma cell othandizira
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Post Nthawi: 2024 - 12 - 05 15:07:02
