Chiyambi
Munda wamankhwala othandizirana achitapo kanthu pazaka khumi zapitazi, makamaka pakukula ndi kugwiritsa ntchito ma cell achilengedwe (NK). Ma cell amtunduwu, omwe amatenga mbali yofunika kwambiri yoteteza thupi, asamakayike popanga mathandizo a buku la khansa ndi matenda ena. Nkhaniyi ikukhudza zamkati mwa kuchuluka kwa khungu la NK, gawo la K562 maselo opanga, kufunikira kwa ma cytokines, komanso mayendedwe amtsogolo kwa ma cell a NK. Kuphatikiza apo, timakhudza kufunika kwaChovala cha Cell DNA pokonzekerapakuwonetsetsa kuti ndi chitetezo cham'manja.
Mwachidule za kuchuluka kwa cell
● Kufunika kwa cell
Kukula kwa maselo a NK ndi zida zodalirika m'munda wa mankhwalawa, zomwe zimapangitsa kuti ma cell a NK azichiritse. Izi zikuthandizira kupanga magawo okwanira maselo a NK omwe amafunikira pakugwiritsa ntchito mankhwala, motero amalimbikitsa azachipatala awo.
● Zigawo za zida
Nthawi zambiri, kuchuluka kwa maselo a NK kukhala ndi ma rentints omwe akuphatikiza ma cell odyetsa, ma cytokines, komanso cell ya cell yosewerera. Izi zigawozi zimagwira ntchito synergustilly kuti mupange malo abwino a khungu la NK za kuchuluka kwa maselo a NK ndi kutsegula, kuwunika zokolola zambiri komanso zoyera.
Udindo wa maselo a Trothoblast mu Nk Kuyambitsa
● makina a kukondoweza
Maselo a Trohoblast amalumikizana ndi maselo a NK nthawi ya m'mimba, akusewera mbali yofunika kwambiri pakusintha ntchito yawo. M'malingaliro a kuchuluka kwa maselo a NK, maselo a Thihoblast amatha kuyambitsa maselo a NK kudzera mu receptor - Biondand Kugwirizana, kulimbitsa ntchito zawo, kulimbikitsa ntchito zawo, kulimbitsa zochitika zawo zapa cytototoxic ndi kuchuluka kwawo.
● Ubwino wogwiritsa ntchito maselo a Troboblast
Kugwiritsa ntchito maselo a Trothoblast mu NK maselo kumapereka njira yothandiza kwambiri yolimbikitsa ya NK, yomwe mwina imatha kutsogolera maselo okhala ndi magwiridwe antchito apakati poyerekeza ndi zomwe zimakulitsidwa ndi njira zopangira.
Maselo a K562 mu NK Cell Cell
● Mawu a Cytokine mu K562 maselo
Maselo a K562 nthawi zambiri amagwiritsidwa ntchito ngati maselo odyetsa mu NK Kukula kwa maselo. Amasinthidwa kufotokoza ma cytokines monga Il - 15 ndi Il - 21, zomwe ndizofunikira kwambiri kwa maselo a NK ndi kutsegula.
● Mavuto a Irradiation ndi Inactation
Irradiation kapena itactivation ya K562 maselo awo asanagwiritsidwe ntchito ngati maselo odyetsa amathandizira kuchuluka kosafunikira, kwinaku akuberekabe kukulitsa maselo a NK.
Kukula kwa Cytokines mu NK Kuyambitsa
● Il - 21 ndi zotsatira zake
Ma cytokines ngati Il - 21 ndi ofanana ndi chiwonetsero cha maselo a NK. - 21 Osangolimbikitsa kuchuluka kwa khungu komanso kumawonjezera ntchito zawo za cytotootootic, ndikupangitsa kuti ikhale gawo lofunikira la kuchuluka kwa khungu la NK.
● Njira zosayimira
Ma cytokines amagwira ntchito kudzera munjira zosiyanasiyana zowunikira kuti ayambitse maselo a NK. Kuzindikira njirazi ndikofunikira kuti mutseke ma protocol a NK ndikuwongolera njira zawo.
Nk maselo a NK: Umbilical chingwe motsutsana ndi magazi otumphulika
● Kufanizira zokolola zam'manja
Chingwe cha Umbilical magazi ndi gwero lambiri la maselo a NK, nthawi zambiri amapereka manambala apamwamba poyerekeza ndi magazi opotoka. Komabe, kusankha pakati pa zinthuzi kumadalira zofunikira pa ntchito yochizira.
● Chiyero ndi mtundu wa ma cell a NK
Magwero onsewa amatha kupereka - maselo apamwamba a NK, koma ma protocors okumbika amatha kukhala osiyana. Kuonetsetsa kuti makonzedwe ndi magwiridwe antchito okumbidwa a NK ndiofunikira kuti akwaniritse zotsatira zoyipa.
Kugwiritsa ntchito mgalimoto - kukonzekera kwa cell cell
● Miyeso yagalimoto - maselo a NK
Galimoto - maselo a NK amapangidwa kuti afotokoze chimbudzi, zomwe zimawalimbikitsa kuti ayang'anire maselo a khansa. Kukonzekera kwa galimoto - maselo a NK kumaphatikizapo kusintha kwa majini ndi kukula komwe kumatsimikizidwa ndi ma kilogalamu apadera.
● Ubwino pa njira zachikhalidwe
Galimoto - maselo a NK amapereka zabwino zambiri pa njira zachikhalidwe, kuphatikizapo chiopsezo cha kuchepetsa - Valani - matenda otenga nawo gawo ndi kuthekera kwa khansa yambiri ya Argnans.
Kukula kwa ma cell kwa cell
● nthawi ndi zothandiza pakukula msanga
Kupanga njira zokwanira za mankhwala a NK ndikofunikira kuti muchepetse nthawi ndi mtengo m'magawo achitukuko. Protocol yokonza ndi kukongoletsa okonzeka - ku - Gwiritsani Ntchito Kukula kwa Manja kumatha kufupikitsa izi.
● Malingaliro okulitsa malonda a cell
Njira monga zokhazokha, kukonza dongosolo, komanso kugwiritsa ntchito ma microbial - Zosintha zaulere ndizofunikira kuti muthe kukonza mtundu ndi kusasinthika kwa malonda a foni.
Kukulitsa chiyero ndi zokolola za maselo a NK
● Maluso kuti akwaniritse chiyero chachikulu
Njira monga kutuluka kwa cytometry - Kusanjana kwa maginito ndi maginito kumagwiritsidwa ntchito kuti akwaniritse zoyera kwambiri m'maselo akukulitsa kwa NK, zomwe ndizofunikira pakugwiritsa ntchito matenda.
● Mavuto ndi mayankho mu ambiri - Kukula kwa Scal
Kuchepetsa kukula kwa khungu la NK kumabweretsa zovuta monga kusuntha kwa maselo ndi magwiridwe antchito. Zojambula zatsopano za bioarecactor ndi njira zowunikira mosalekeza ndizothetsa mavutowa.
Ubwino Wogwiritsa Ntchito Nk Cell Base
● Udindo wa kuchuluka kwa cell ndi kutsegula
NK Cell basel sing'anga imapangidwa makamaka kuti ithandizire kukula ndi kutsegula kwa maselo a NK, ndikuwonetsetsa kugwiritsa ntchito njira zosiyanasiyana kudutsa ma protocol osiyanasiyana.
● Kugwirizana ndi ma cytokines osiyanasiyana
Kufanizira kwa sing'anga yokhala ndi ma cytokines osiyanasiyana kumalola kusinthasintha pakupanga kwa khungu la NK kuti likwaniritse zosowa zinazake zochizira.
Mayendedwe amtsogolo kwa ma cell a NK
● zochitika zam'madzi mumankhwala
Munda wa mankhwala a NK cell akutuluka mwachangu, ndikutuluka komwe akutuluka pakuthana ndi tanthauzo, kuchepetsa - zoyipa, ndikusintha chipongwe chamankhwala.
● Kupita patsogolo kwa maselo a NK
Kukula kwa mtsogolo kumatha kuphatikizapo kufalikira kwa dolor NK maselo, kuphatikiza njira zina zamthupi, komanso zochizira zam'madzi za NK zam'madzi zimagwirizana ndi maluso awo.
Kuonetsetsa kuti cell cess DNA Prevoseng Kits
● Kufunika kwa cell
Cell Cell DNA Ponekisi yokonzekera zikuluzikulu zimafunikira mu cell mankhwala kuti akwaniritse ndikutha zowonjezera zotsalira za Cell DNA. Izi zitsimikiziro onetsetsani kuti chitetezo cham'madzi cha mankhwala a cell pochepetsa chiopsezo cha kuipitsidwa.
● Ntchito zopanga ndi othandizira
Opanga ndi othandizira a cell DNA Prevoseng Kits, mongaBuluuni, kusewera gawo lofunikira pakupereka wodalirika komanso wapamwamba - Makampani abwino. Izi ndi zofunika kuti tisunge umphumphu wa ma produsals mankhwala.
Mapeto
Malo a mankhwala am'manja amasintha mosalekeza, ndi maselo a NK patsogolo pa izi. Kuphatikiza kwa kuchuluka kwa chipachipadera chachipamwamba, kuzindikira kwa cytokine, ndi zida zabwino zotsimikizika ngati cell dna plass dro place ikuyendetsa machiritso otetezeka komanso ochiritsa. Makampani akamapita patsogolo, mgwirizano pakati pa ofufuzawo, azachipatala, ndi opanga adzakhala kiyi kuti akutsegule kuthekera kotheka kwa maselo a NK.
Jiangsu Hillgene, pansi pa Blank BlueKit®, amayimilira kutsogolo kwa ma cell a celluur. Ndili ndi likulu lake ku SUZHU ndi kupanga mikono ku China ndi North Carolina, mapiri aku Carolina, mapiri akupita ku upainiya akuchita zinthu zopangira manyuchi ndi ma cell. Kudzipereka kwa zowongolera zapamwamba, zinthu za buluu® kutsimikizika kutsimikizika ndikuwongolera kwa ma cell ochizira bwino kwambiri pagalimoto yopambana - T, TCR - Th cell netle. Ndi masomphenya olimbikitsa a foni yam'manja, mapiri amadzipereka kuti apitirize kukothara ma cellular solide padziko lonse lapansi.
Post Nthawi: 2024 - 12 - 13 15:31
