PICHIA SWRAMORIS HCP (Host Cell Protein) Realidual Cov Khoom Siv Nrhiav
PICHIA SWRAMORIS HCP (Host Cell Protein) Realidual Cov Khoom Siv Nrhiav
Piv txog Pichia pastoris - Tsis muaj tus ntoo khaub lig - Assivity nrog Cho / HCP
Piv txog Npaj - rau - siv - Tsis muaj cov qauv pretricatment yuav tsum muaj
Siv
Cov khoom siv no yog tsim los rau cov tshuaj tsuag kom paub kom tau pom kev tswv ntawm Host Cell Protein (HCP) cov khoom seem hauv biopharmaceutical muab los ntawm Pichia pastoris Cov kab mob (piv txwv li, GS115, X33), siv ib qho - Step enzyme - Txuas immunosorbent assay (Elisa). Rau kev tshawb fawb siv nkaus xwb. Tsis yog rau kev kuaj mob lub hom phiaj.
Lub hauv paus ntsiab lus kev pom
Cov Khoom Siv Ua Haujlwm A Sandwich Elisa Txoj kev:
-
Daim plhaub: Microplate Wells yog Prece - Coated nrog Yaj Nrog Yaj Polyclonal Antibodies tiv thaiv Pichia pastoris HCPs.
-
Tsim kom lojcation: Cov qauv / cov calibrators tau ntxiv, ua raws HRP - cov lus sib piv ntawm cov yaj Polyclonal anti -Pichia pastoris HCP cov tshuaj tiv thaiv.
-
Kev pom: Tom qab ntxuav, tmb substrate yog ntxiv rau kev txhim kho xim. Cov tshuaj tiv thaiv tau nres, thiab nqus tau ntsuas 450 nm.
-
Meem: HCP kev pom zoo tau suav los ntawm kev nkhaus txheem (nqus tau α hcp siab).
Cov Nta Tseem Ceeb
-
Tsis muaj cov qauv pretricatment yuav tsum muaj (Tsuas yog siv tau qhov siv tau).
-
Streamlined raws tu qauv: Cov kauj ruam tsawg kawg, cov txiaj ntsig sai (<2 teev).
-
Siab muaj feem ntau thiab kev ntseeg tau: Optimized rau Pichia pastoris HCPs.
Kev Qhia Ntawv
-
Tshaj mus kuaj: Cell Kev lis kev cai supernatants, lim tej biologics.
-
Dynamic ntau: 1-100 ng / ml (raug).
-
Kev tswj hwm txoj cai: Haum rau cov txheej txheem kev tsim kho thiab GMP kev ntsuam xyuas ntau.
Cov txheej txheem siv1. Sib npaug
-
Nqa tag nrho cov khoom siv rau chav sov (≥20 min).
2. Qauv / Antibody Ntxiv-
Ntxiv cov qauv / cov ntsuas hluav taws xob: 100 μl / Zoo
-
Ntxiv 1x enzyme - conjugated antibody: 100 μl / Zoo (saib cov lus dab neeg ①)
3. Txhim Kho-
Txo ntawm RT, tiv thaiv los ntawm Lub Teeb, 600 RPM rau 3 h (saib cov lus dab neeg ③)
4. Ntxuav-
Ntxuav 1: 300 μl / Zoo 1x ntas (ntsug phaj txoj hauj lwm)
-
Ntxuav 2 - 5: 300 μl / Zoo 1x ntas, 5 tag nrho ntxuav
-
Patb qhuav: Tom qab ntxuav tas, invert phaj ntawm nqus ntawv
5. Tmb substrate kev txhim kho-
Ntxiv TMB: 100 μl / Zoo
-
Pab tsa: 10 min ntawm rt (tiv thaiv los ntawm lub teeb)
6. Tsis txhob siv cov tshuaj tiv thaiv & nyeem ntawv-
Ntxiv Nres Nres Tshuaj: 50 μl / zoo
-
Sawv ntsug: 5 min
-
Nyeem: OD ntawm 450 nm (siv 620 nm)
-
| Ib feem | Khoom No. | Kev qhia tshwj xeeb | Kev piav txog |
|---|---|---|---|
| Ua ntej - Coated anti - PICHIA HCP Microplate | PNA015 | 8 KUA × 12 strips | Ua ntej - coated nrog yaj polyclonal anti -Pichia pastoris HCP cov tshuaj tiv thaiv. Kaw rau hauv ntawv nyiaj pouches nrog desiccant. Khw muag khoom ntawm lub teeb. |
| PICHIA HCP Calibrator | Pnb015 | 2 vials | Lyophilized hmoov. Reconste nrog 500 μl calibrator diluent (pnc002). Cia 5-10 min rau kev xaus (kev daws teeb meem yuav tsum meej). Khw muag khoom ntawm lub teeb. |
| Calibrator diluent | Pnc002 | 1.5 ml × 1 raj | Kev daws teeb meem rau kev rov ua dua rau PICHIA HCP Calibrator. |
| Assin diluent | Lub tsev ua mov | 25 ml × 2 hwj | Rau cov tshuaj calibrators, cov qauv kuaj, thiab enzyme - conjugated tshuaj tiv thaiv antibodody. Siv tau cov txiaj ntsig zoo rau cov qauv tshiab. |
| Ntxuav tsis (10 × zoo) | Pnf001 | 25 ml × 2 hwj | Rau Phaj Ntxuav. Cov muaju yuav ua tiav ntawm qhov kub tsawg-yaj ntawm 37 ° C ua ntej siv. Dilute 10 × nrog ultrapure dej. |
| HRP - Congugated anti - PICHIA HCP antibodody (100 ×) | Hnab sis / | 120 μl × 1 raj | Yaj polyclonal tshuaj tiv thaiv. Dilute 100 × nrog assay diluent ua ntej siv. Khw muag khoom ntawm lub teeb. |
| Tmb substrate | Pnd004 | 12 ml × 1 lub raj mis | Equilibrate rau chav sov (≥20 min) ua ntej siv. Khw muag khoom ntawm lub teeb. |
| Nres daws | Pni002 | 6 ML × 1 lub raj mis | Muaj hcl. Hnav goggles thiab zam kom tsis txhob sib chwv txiv. |
| Cov yeeb yaj kiab sealing | Pnk001 | 3 daim ntawv qhia | Rau npog microplate strips kom tiv thaiv kev sib kis thiab evaporation. |
Cov ntaub ntawv xa khoom
Peb muab thauj hauv tub yees rau txhua daim xaj. Feem ntau, koj qhov kev txiav txim yuav tuaj txog hauv 5 - 7 hnub ua haujlwm hauv Tebchaws Meskas thiab hnub nyoog li ntawm 10 hnub ua haujlwm rau lwm lub tebchaws. Txawm li cas los xij, thov nco ntsoov tias kev xa khoom rau cov chaw deb nroog yuav siv sijhawm me ntsis.
Lub sij hawm xa khoom: cov xaj feem ntau ua tiav tsis pub dhau 1 - 3 hnub ua haujlwm. Thaum koj xaj tau raug xa, koj yuav tau txais email kabke nrog cov ntaub ntawv taug qab.
Cov Ntaub Ntawv Tseem Ceeb
Kev txiav txim siab ua: tom qab txiav txim tau them nyiaj, peb lub npe xav tau qee lub sijhawm los ua koj daim ntawv xaj. Koj yuav tau txais kev ceeb toom ib zaug koj daim ntawv xaj tau raug xa tawm.
Lub sij hawm xa tuaj: Feem ntau, lub pob yuav xa tawm hauv lub sijhawm kwv yees ntawm tuaj txog. Txawm li cas los xij, hnub xa tawm tiag tiag tuaj yeem raug cuam tshuam los ntawm kev npaj davhlau, huab cua, thiab lwm yam sab nraud. Lub sijhawm khoom xa tuaj yuav ntev dua li ib txwm rau kev txiav txim uas muaj cov khoom siv ua ntej lossis cov khoom siv. Thov saib cov ntaub ntawv taug qab rau cov hnub xa tau tshaj plaws.
Cov teeb meem thauj khoom: Yog tias koj pom tias koj pob tsis tau xa hauv lub sijhawm tau teev tseg; Cov ntaub ntawv nrhiav cov ntaub ntawv qhia tau hais tias pob khoom tau xa tawm tab sis koj tsis tau txais nws; Los yog koj pob nrog cov khoom tsis raug lossis tsis raug lossis lwm yam teeb meem logistics, thov hu rau hnub thov cov qhua hauv 7 hnub kom peb tuaj yeem ua cov teeb meem no kom sai.
Kev Taug Xyuas
Thaum koj xaj tau xa, koj yuav tau txais email nrog tus lej nrhiav thiab qhov txuas los taug qab koj cov zaub mov xa tuaj.
Koj tuaj yeem taug qab koj cov xaj ncaj qha rau ntawm peb lub vev xaib los ntawm kev nkag mus rau hauv koj tus lej nyiaj thiab saib koj keeb kwm kev txiav txim.
Kev xa khoom xa tawm
Thov sau rau hauv txoj kev chaw nyob hauv kev nthuav dav, tsis yog po box lossis chaw nyob tub rog (apo). Txwv tsis pub, peb yuav tsum tau siv EMS rau kev xa khoom (nws qeeb dua lwm tus, noj ib ncig 1 - 2 hlis lossis ntev dua).
Cov Haujlwm Customts thiab Kev Se Se
Thov nco ntsoov tias txhua txoj haujlwm kev lis kev cai, cov se, lossis import cov nqi them rau thaum xa khoom yog lub luag haujlwm ntawm tus yuav. Cov nqi no sib txawv nyob ntawm lub teb chaws lub teb chaws thiab tau txiav txim siab los ntawm cov tub ceev xwm hauv zej zog.
Los ntawm kev yuav khoom los ntawm peb lub vev xaib, koj pom zoo them txhua txoj haujlwm lossis cov se uas cuam tshuam nrog koj daim ntawv xaj. Peb tsis yog lub luag haujlwm qeeb rau kev qeeb los ntawm kev ua kev lis kev cai.
Pob Cov Cai Ntim Khoom
Thaum koj cov xaj tau tuaj txog ntawm cov khoom xaiv xaiv lossis qhov chaw xa khoom, thov xyuas kom paub cov khoom ntawv sai. Yog tias cov pob tsis tau nqa nyob hauv lub sijhawm teem sijhawm, peb yuav xa cov ntawv ceeb toom ntawm email lossis sms. Txawm li cas los xij, yog tias pob ntawv tsis tau sau nyob rau hauv lub sij hawm teev tseg, thiab kev poob lossis kev puas tsuaj tshwm sim vim li cas, tus yuav yuav tau ua lub luag haujlwm. Peb ua siab zoo rau koj kom sau koj pob kom sai kom zam rau txhua yam teeb meem.
NCO TSEG: Raws li peb cov khoom poob hauv qab qeb tshwj xeeb, rov qab thiab cov nyiaj them rov qab tsis tau txais.
