Nk thiab til cell nthuav dav (K562 Faib Cell)
Nk thiab til cell nthuav dav (K562 Faib Cell)
✔ Cov ✔ Ua ntej - irradiated, multives ntau - cytokine txhim kho rau cov nke superior nk / til expansion.
✔ Cov ✔ Siv tau rau ob leeg cov ntshav thiab pbmc qhov chaw - Tsheb - Npaj.
✔ Cov ✔ Pre - Tsim nyog rau Ind Kev Xa nrog cov ntaub ntawv tag nrho.
Txheej txheem cej luam:
Tus Nk / til expansion reagent muaj engineered, irradiated k562 feeder hlwb Qhia IL - 21 thiab lwm yam cytokines. Thaum ua ke nrog cov qauv ua npaws ntawm tes, lub kaw lus no ua haujlwm:
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Siab - efficiency expansion ntawm NKOJ COB THIAB TILS los ntawm pbmcs lossis cov ntshav -
Siab - Purity Haiv Neeg (> 90% lub hom phiaj hlwb)
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Ceev ceev, nkag mus rau kev ua kom nkag tau Nyob rau hauv optimized cytokine signaling
Cov Ntawv Qhia Tseem Ceeb
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Ua kom muaj & nthuav dav ntawm Nki hlwb, tsheb - noks, thiab tils
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Kev Tsim Kho Cov Txheej Txheem: Thaum ntxov - theem kev txhim kho ntawm kev kho mob cell
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GMP Raug: Txhawb nqa Indian - Ua Haujlwm Kawm thiab Ncej - Kev Pom Zoo Cov Txheej Txheem Hloov
Kev Tswj & Kev Ua Tau Zoo
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GMP - Ua Raws txoj kev
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Xwm yeem, siab - tawm los nthuav (mus txog 1000 - Fold)
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Ua ntej - siv tau rau kev siv tshuaj kho
Datasheet
Nky cell kev nthuav dav ua haujlwm thiab kev sib kis thoob plaws ntau tus neeg pab nyiaj
Pub rau hauv - Sonor hloov pauv hauv nk ntawm nkuab cell purity
Kev ua tiav cov txiaj ntsig viral e - Hancerc ntawm Lentiviral hloov chaw nyob rau hauv NK Cells
NKY COB: VIRAL E - HALCER OPERPORFORFORS ntawm MOI 1 LOOJ YIM: NIGE CYTOMETRY NTAWM IL - 15 KK hlwb nrog Viral E - Hancerc
Vaj Huam Sib Luag: Ntiv Cytometry ntawm IL - 15 Nk Hlis Tsis Muaj Hloov Txhim Kho Kom Haum
Viral E - Hancer Vs. Cov Neeg Sib Tw: T - Transduction ntawm MOI 5 Laug: ntws cytometry ntawm tsheb - TYBS nrog Viral E - Hancerc
Txoj cai: ntws cytometry ntawm tsheb - t hlwb tsis hloov pauv hloov kho
Li cov nyhuv sib xws ntawm veral e - yancer A + Viral A + tus kab mob E - Hancer D sib xyaw rau Lentiviral Transduction hauv HSCS
Kev Tshaj Tawm ntawm HSC Hloov Chaw Ua Tau Zoo: Viral E - Hancere E - Hancerd Sib Tw Tiv Thaiv Kev Sib Tw (Moi = 5)
| Muaj pes tsawg tus lej | Lub npe ntawm Reagentand consumables | Muab tshuaj (rau ~ 1 - 2e6 PBMC Pib) |
| 1 | Ntshav qab zib - Dawb Nruab Nrab | 2 l |
| 2 | IL - 2 | 4 sticks (200,000 iu / pas) |
| 3 | 0.9% NacL Txhaj | ~ 500 ml |
| 4 | 20% tib neeg txog ntshav albumin | ~ 15 ml |
| 5 | Ficoll Lymphocyte Kev daws teeb meem | Tom ntej no 25 ml |
| 6 | NC - 200 suav chamber (lossis sib npaug) | Raws li xav tau |
| 7 | 6 - Lub Phaj Zoo | 1 |
| 8 | T25 RERDK | 1 |
| 9 | T75 RERK | 1 |
| 10 | T175 Flask | 2 |
| 11 | 640 CMO CELLY LUB HNAB | 2 |
| 12 | 15 ml Centrifuge raj | 1 |
| 13 | 1.5 ml Centrifuge raj | Raws li xav tau |
| 14 | 10 ml / 25 ml pipettes | Raws li xav tau |
| 15 | 1 ml / 200 μl Pipette Cov Lus Qhia | Raws li xav tau |
Kev qhia tshwj xeeb
| Ua Kom Parameter | Kev qhia tshwj xeeb |
| Nk / tsheb - NK expansion quav | > 5,000 × (14 hnub) |
| Kev txawj ntse ntawm tes | ≥90% |
| Cell Purity (CD56⁺ / CD3⁻) | > 95% |
Cov ntaub ntawv xa khoom
Peb muab thauj hauv tub yees rau txhua daim xaj. Feem ntau, koj qhov kev txiav txim yuav tuaj txog hauv 5 - 7 hnub ua haujlwm hauv Tebchaws Meskas thiab hnub nyoog li ntawm 10 hnub ua haujlwm rau lwm lub tebchaws. Txawm li cas los xij, thov nco ntsoov tias kev xa khoom rau cov chaw deb nroog yuav siv sijhawm me ntsis.
Lub sij hawm xa khoom: cov xaj feem ntau ua tiav tsis pub dhau 1 - 3 hnub ua haujlwm. Thaum koj xaj tau raug xa, koj yuav tau txais email kabke nrog cov ntaub ntawv taug qab.
Cov Ntaub Ntawv Tseem Ceeb
Kev txiav txim siab ua: tom qab txiav txim tau them nyiaj, peb lub npe xav tau qee lub sijhawm los ua koj daim ntawv xaj. Koj yuav tau txais kev ceeb toom ib zaug koj daim ntawv xaj tau raug xa tawm.
Lub sij hawm xa tuaj: Feem ntau, lub pob yuav xa tawm hauv lub sijhawm kwv yees ntawm tuaj txog. Txawm li cas los xij, hnub xa tawm tiag tiag tuaj yeem raug cuam tshuam los ntawm kev npaj davhlau, huab cua, thiab lwm yam sab nraud. Lub sijhawm khoom xa tuaj yuav ntev dua li ib txwm rau kev txiav txim uas muaj cov khoom siv ua ntej lossis cov khoom siv. Thov saib cov ntaub ntawv taug qab rau cov hnub xa tau tshaj plaws.
Cov teeb meem thauj khoom: Yog tias koj pom tias koj pob tsis tau xa hauv lub sijhawm tau teev tseg; Cov ntaub ntawv nrhiav cov ntaub ntawv qhia tau hais tias pob khoom tau xa tawm tab sis koj tsis tau txais nws; Los yog koj pob nrog cov khoom tsis raug lossis tsis raug lossis lwm yam teeb meem logistics, thov hu rau hnub thov cov qhua hauv 7 hnub kom peb tuaj yeem ua cov teeb meem no kom sai.
Kev Taug Xyuas
Thaum koj xaj tau xa, koj yuav tau txais email nrog tus lej nrhiav thiab qhov txuas los taug qab koj cov zaub mov xa tuaj.
Koj tuaj yeem taug qab koj cov xaj ncaj qha rau ntawm peb lub vev xaib los ntawm kev nkag mus rau hauv koj tus lej nyiaj thiab saib koj keeb kwm kev txiav txim.
Kev xa khoom xa tawm
Thov sau rau hauv txoj kev chaw nyob hauv kev nthuav dav, tsis yog po box lossis chaw nyob tub rog (apo). Txwv tsis pub, peb yuav tsum tau siv EMS rau kev xa khoom (nws qeeb dua lwm tus, noj ib ncig 1 - 2 hlis lossis ntev dua).
Cov Haujlwm Customts thiab Kev Se Se
Thov nco ntsoov tias txhua txoj haujlwm kev lis kev cai, cov se, lossis import cov nqi them rau thaum xa khoom yog lub luag haujlwm ntawm tus yuav. Cov nqi no sib txawv nyob ntawm lub teb chaws lub teb chaws thiab tau txiav txim siab los ntawm cov tub ceev xwm hauv zej zog.
Los ntawm kev yuav khoom los ntawm peb lub vev xaib, koj pom zoo them txhua txoj haujlwm lossis cov se uas cuam tshuam nrog koj daim ntawv xaj. Peb tsis yog lub luag haujlwm qeeb rau kev qeeb los ntawm kev ua kev lis kev cai.
Pob Cov Cai Ntim Khoom
Thaum koj cov xaj tau tuaj txog ntawm cov khoom xaiv xaiv lossis qhov chaw xa khoom, thov xyuas kom paub cov khoom ntawv sai. Yog tias cov pob tsis tau nqa nyob hauv lub sijhawm teem sijhawm, peb yuav xa cov ntawv ceeb toom ntawm email lossis sms. Txawm li cas los xij, yog tias pob ntawv tsis tau sau nyob rau hauv lub sij hawm teev tseg, thiab kev poob lossis kev puas tsuaj tshwm sim vim li cas, tus yuav yuav tau ua lub luag haujlwm. Peb ua siab zoo rau koj kom sau koj pob kom sai kom zam rau txhua yam teeb meem.
NCO TSEG: Raws li peb cov khoom poob hauv qab qeb tshwj xeeb, rov qab thiab cov nyiaj them rov qab tsis tau txais.
