Dab tsi yog qhov sib txawv ntawm trypsin thiab lwm yam kev tiv thaiv?

Taw qhia rau cov kev tiv thaiv hauv kev siv hluav taws xob

Kev tiv thaiv, muaj ntau pab pawg enzymes, yog pivotal nyob rau ntau cov txheej txheem biological roj ntsha, los ntawm kev zom rau ntawm tes teeb liab. Lawv ua haujlwm los ntawm kev sib luag ntawm cov leeg peptide hauv cov protein, yog li ua si lub luag haujlwm tseem ceeb hauv kev tswj hwm ntawm cov vaj tse ntawm lub tsev pheebsab. Cov neeg muaj kev tiv thaiv tau faib raws lawv cov txheej txheem catalytics thiab cov txheej txheem txheej txheem, nrog cov kev tiv thaiv syryin zoo li Trypsin yog ntawm kev kawm tshaj plaws. Cov lus no tshuaj xyuas cov cwj pwm tshwj xeeb ntawm cov cwj pwm tshwj xeeb ntawm kev sib piv rau lwm cov kev tiv thaiv, xav txog cov qauv, ua haujlwm, thiab kev siv.

Trypsin: tus qauv kub hauv cov proteomics

Lub luag haujlwm ntawm Trypsin hauv Mass Spectrometry

Trypsi ua rau cov proteomics vim nws qhov tshwj xeeb thiab kev ua tau zoo hauv cov hlua khi ntawm lub lysine thiab arginine seem. Qhov tshwj xeeb no tshwm sim hauv cov thooj nrog tus nqi zoo ntawm lawv c - Terminus, kom zoo dua rau cov loj spectrometry (MS) tsom xam. Hauv cov proteomics, Trypsin lub peev xwm los tsim kom muaj txiaj ntsig zoo ntev ua rau kev tshawb fawb txog cell, qhov twg yog qhov tseem ceeb protein uas tsim nyog.

Cov txiaj ntsig zoo hauv cov cim protein

Proteomics hnyav tshaj plaws ntawm Trypsin cov khoom tshwj xeeb rau cov cim protein. Kev tshawb fawb pom tias Trypsin tuaj yeem txheeb xyuas dua 80% ntau cov protein ntau dua li muaj kev tiv thaiv ntau yam, ua rau nws tseem ceeb rau cov kws tshawb nrhiav thiab cov neeg muag khoom hauv qhov chaw. Nws lub luag haujlwm nthuav dav los ntawm kev nkag siab tus kab ke rau kev siv hluav taws xob rau kev txhim kho cov kev kho, xws li kev kho mob cell.

Cov qauv kev coj ua ntawm Trypsin

Lub Chaw Haujlwm thiab Catalynestic Mechanism

Cov catalytic kev ua tau zoo ntawm trypsin stems los ntawm nws zoo - cov khoom txhais, ua kom muaj kev sib tw catalytic, muaj kev sib tw ntawm coverine, thiab cov aspartate. Qhov kev teeb tsa no tau muab cov kev xav tau peptide, ib qho yuav tsum tau ua rau cov neeg xa khoom rau cov neeg xa khoom rau kev tshawb fawb thiab kev siv cov ntawv thov. Qhov tshwj xeeb ntawm enzyme yog ntaus nqi mus rau lub xub ntiag ntawm qhov tsis zoo them aspartate cov chaw nyob, uas nyiam cov nqi uas muaj zoo thiab suav.

Isoforms ntawm Trypsin

Trypsin muaj nyob rau hauv ntau cov isoforms, suav nrog Cationic, anionic, thiab mesotrypsvelecogen. Txhua OFOrd pabcuam cov luag haujlwm txawv, nrog Cationic Trypsin yog qhov muaj ntau tshaj plaws hauv tib neeg lub pancreas. MesoTryPSin tsis tuaj yeem ua rau nws tsis kam rau inibitors, ua ib lub luag haujlwm tshwj xeeb hauv kev ua tsis ncaj ncees - Cov khoom noj muaj txiaj ntsig nplua nuj nplua mias. Nkag siab cov ISOForms yog qhov tseem ceeb rau cov xwm txheej uas muaj feem cuam tshuam nrog enzyme ntau lawm rau siv tshuaj kho mob.

Lwm cov kev tiv thaiv thiab lawv cov tshwj xeeb tshaj plaws

Kev sib piv nrog aspn thiab gluc

Aspn thiab Gluc Phiaj Acidic Amino cov khoom seem, muab cov ntaub ntawv txuam nrog uas muab los ntawm trypsin. Cov kev tiv thaiv cov protective complex Peptide mixtures haum rau kev ntsuas tshwj xeeb hauv cov proteomics. Txawm li cas los xij, lawv cov dav dav tshwj xeeb tau ua rau cov cim protein txo rau trypsin.

Chinotrypsin thiab qhov chaw muaj kev tiv thaiv tshwj xeeb

Chymotterin txawv los ntawm Trypsin los ntawm kev sib tw loj hydrophobic seem, xws li Tryptophan thiab Tyrosine. Thaum nws tuav cov catalytic efficiency, nws cov kev siv tau feem ntau txwv rau qee cov ntawv thov. Tawv - Muaj kev tiv thaiv tshwj xeeb xws li proteinase k tsim cov kev sib tw pustle, tshwj xeeb yog siv ua ke nrog Trypsin.

Ua ntej ntawm cov txheej txheem thev naus laus zis nrog trypsin

Txhim Kho Protein Qhia Txog

Muaj kev noj coj los ntawm kev noj mov, cuam tshuam nrog kev siv Trypsin ua raws li lwm qhov kev tiv thaiv, txhim kho txhim kho protein cim. Piv txwv li, predigesting nrog trypsin tuaj yeem ua rau cov cim protein ntau rau cov proteinase K ntawm 731%. Rau cov ntawv kho mob cell, no ua rau lub synnergy tso cai rau cov proteomic ntau dua, qhov tseem ceeb rau kev nkag siab cellular.

Kev tiv thaiv ntawm peptides hauv kev lis kev zom zaub mov

Kev tiv thaiv me me tsim los ntawm Trypsin muaj kev tiv thaiv los ntawm kev zom ntxiv rau cov txheej txheem ntawm cov txheej txheem, ua rau muaj kev nyuaj dua li kev kwv yees los ntawm kev txheeb xyuas silico. Cov txheej txheem no tau txais txiaj ntsig zoo rau cov neeg xa khoom muab cov kev pabcuam muaj kev sib tw, raws li nws ua kom muaj cov cim kev paub txog protein thiab qhov cim.

Tshwj xeeb tshaj tawm ntsig txog qhov dav tshwj xeeb tiv thaiv kev tiv thaiv

Cov kev tiv thaiv nrog qhov tshwj xeeb muaj tseeb, zoo li trypsin, yog kev nyiam vim lawv lub peev xwm los ua cov khoom siv tsis paub tseeb thiab tswj tau peptide peptide. Hauv kev sib piv, qhov tseeb muaj kev tiv thaiv tshwj xeeb hauv cov nyom sib xyaw, cov ntaub ntawv ua haujlwm tsis zoo. FACTORIES uas tsim cov enzymes rau kev tshawb fawb yuav tsum xav txog cov kev sib txawv no kom tau raws li cov kev xav tau ntawm cov kev ua proteolytic ua rau muaj ntau yam kev siv.

Lub luag haujlwm ntawm kev ua yeeb yam hauv kev tiv thaiv kev ua haujlwm

Protease proteion precursor activation

Cov kev tiv thaiv feem ntau cov khoom ua ke ua cov tsis muaj zog rau kev rhuav tshem kev rhuav tshem cov protein uas tsis zoo. Trypsinogen, ua kom muaj nyob rau hauv lub plab hnyuv me, qhia txog cov ntsiab lus no zoo. Nkag siab Zymogen ua kom yog qhov tseem ceeb rau cov kev tiv thaiv kev tiv thaiv rau kev siv rau kev kho kom siv, ua kom cov enzymes uas nquag siv.

Cov kev cai los ntawm Enterokinase

Enterokinase plays lub luag haujlwm tseem ceeb hauv kev ua kom nruj ntawm Trypsinogen, txhawb qhov tseem ceeb ntawm kev ua kom tiav. Qhov sib npaug ntawm kev ua si zoo nkauj rau kev ua kom zoo nkauj ua kom muaj txiaj ntsig zoo hauv lub cev, ib qho kev ua tau zoo nyob hauv lub cev, ib qho pivotal rau cov neeg xa khoom tshwj xeeb hauv cov khoom siv enzyme.

Protedase inibitors thiab lawv cov kev lom zem biological

Tswj ntawm cov proteolytic kev ua si

Protedition inibitors yog qhov tseem ceeb hauv kev tswj cov proteolytic kev ua ub no hauv kev siv hluav taws xob. Lawv los tiv thaiv kev tswj tsis tau kev ua tsis ncaj ntawm cov protein, ib qho kev sib luag hauv cov khoom muaj zog ntau. Hauv kev kho mob cell, nkag siab qhov kev sib tw no yog qhov tseem ceeb rau kev tsim kho kev kho mob uas cov kev ua haujlwm tiv thaiv kev ua haujlwm zoo.

Protedase inibitors hauv kev kho mob

Protedition inibitors tsis tsuas muaj lub luag haujlwm nyob rau hauv cov kev cai physiological tab sis kuj tseem nyob rau hauv cov ntawv thov kho. Lawv muaj cov txiaj ntsig zoo, xws li kev tiv thaiv hlaus kev puas tsuaj los ntawm cov tshuaj tua kab mob los yog txo cov tumorigenesis pheej hmoo, qhia txog lawv qhov tseem ceeb hauv kev tshawb fawb kho mob thiab kev loj hlob.

Kev lag luam biotechnological ntawm trypsin

Lub luag haujlwm hauv Biotechnology

Trypsin yog dav siv nyob rau hauv cov txheej txheem biotechnological, xws li kev zom thiab muaj protein thiab cell. Nws qhov tshwj xeeb thiab kev ua tau zoo rau nws xaiv tau ntau daim ntawv thov, suav nrog rau hauv cov khoom lag luam ntawm tes, qhov twg prezymatic kev ua yog qhov tseem ceeb.

Blukit muab cov kev daws teeb meem

Cov kab xiav muaj cov kev daws teeb meem zoo kom ua tau raws li kev txwv tsis pub ua rau kev tshawb fawb thiab kev siv cov ntawv thov. Los ntawm kev muab siab - Cov Khoom Zoo Zoo Trypsin thiab lwm yam kev tiv thaiv, xiav kom cov khoom siv tsim nyog los ua lawv cov haujlwm hauv cov proteomics, kev kho mob cell, thiab biotechnology cell. Peb cov kev cog lus rau kev ua tau zoo thiab ua kom muaj kev tsim kho tshiab peb ua tus neeg xa khoom, pab koj ua tiav kev txhim khu kev qha thiab pom zoo hauv koj txoj kev ua haujlwm science.