Taw qhia rau tib neeg IL - 2 kuaj cov khoom siv
● Cov lus txhais thiab tseem ceeb
Sib txuas - 2 (IL - 2) yog ib tug pivotal cytokine hauv tib neeg lub cev tiv thaiv kab mob, feem ntau yog qhov tseem ceeb rau lub cev tiv thaiv kab mob. Cov khoom siv il - 2 Kuaj cov khoom siv yog qhov tseem ceeb ntawm cov cuab yeej siv hauv kev tshawb fawb thiab cov chaw kuaj mob los ntsuas cov il - 2 hauv ntau txoj kev ntsuas roj ntsha. Cov khoom siv no tseem ceeb rau kev nce qib peb txoj kev nkag siab ntawm cov kev kho mob ntawm kev kho mob tshiab, txhim kho cov kev siv ntawm cell kev kho mob.
Qhov tseem ceeb ntawm IL - 2 kuaj pom txuas ntxiv hla ntau daim teb, suav nrog kev txhaj tshuaj tiv thaiv thiab kev nkag siab ntawm kev tiv thaiv kab mob tsis muaj zog tuaj yeem ua rau muaj kev txhim kho hauv kev kho. Raws li Kev Lag Luam Biotech hloov kho, qhov kev thov rau cov meej mom, txhim khu tib neeg il - 2 Nrhiav cov khoom siv los ntawm cov tuam ntxhab thiab cov chaw muag khoom thoob ntiaj teb.
Cov tshuaj siv tshuaj lom neeg siv
Neeg IL - 2 Kuaj Khoom SivS tuaj yeem thov rau ntau txoj kev kuaj mob biological, nrog rau cov ntshav ntshav, ntshav, kab lis kev cai supernatants, thiab cov ntshav tag nrho. Txhua hom piv txwv muab cov kev pom tshwj xeeb thiab cov kev sib tw, ua rau cov versatility ntawm cov khoom siv mus kuaj pom ib qho nqi hluav taws xob. Piv txwv li, tshuaj xyuas kab mob cellatants supernatants tuaj yeem muab cov ncauj lus kom ntxaws ntawm cov cyto, uas yog qhov tshwj xeeb tshaj plaws hauv kev txhaj tshuaj tiv thaiv tshiab.
Mechanism ntawm IL - 2 Nrhiav Kom Tau
Lub luag haujlwm ntawm cov tshuaj tiv thaiv thiab cov enzymes
Cov tub ntxhais ntawm tib neeg IL - 2 kuaj pom cov khoom siv ntawm kev siv tshuaj tua kab thiab cov enzymes kom pom tshwj xeeb thiab ua kom pom tseeb IL - 2 molecules. Feem ntau, cov khoom siv no tau ua ib qho qhaandwich elisa (enzyme - txuas immunosorbent assay) hom ntawv. Hauv cov txheej txheem no, IL - 2 tam sim no nyob rau hauv cov qauv yog cov qauv ntawm ob txheej txheem tiv thaiv: Lub enzyme reacts nrog ib substrate los tsim cov kev hloov xim officimetric uas tuaj yeem ntsuas ntsuas spectrophhotometrically.
Cov qauv no rhiab thiab muaj feem xyuam raws li qhov zoo ntawm cov tshuaj tiv thaiv siv, uas yog vim li cas thiaj xaiv cov khoom siv lossis cov chaw tsim khoom yog qhov tseem ceeb. Cov npe nrov tsim tawm kom ntseeg tau cov tshuaj tiv thaiv cov tshuaj tiv thaiv cov tshuaj tiv thaiv thiab siv tau los muab cov txiaj ntsig tau zoo.
● Siv xim rau hauv kev siv thiab IL - 2 2
Lub hauv paus pob zeb ntawm seimecting il - 2 theem siv cov khoom siv nrhiav tau yog cov xim siv los ntawm enzyme - substrate RAWS. Qhov kev hloov pauv kev hloov xim no yog ncaj qha rau cov concentration ntawm il - 2 hauv cov qauv. Los ntsuas ntsuas qhov nqus ntawm ib qho yoj txawv, cov kws tshawb nrhiav tuaj yeem txiav txim siab rau cov IL - 2 kev xav tau tsim cov qauv nkhaus los ntawm cov muaj ntau.
Cov ntawv thov ntawm IL - 2 Nrhiav Kom Tau
Pro CL T Cell Kev Tsim Kho Cell
Il - 2 muaj lub luag haujlwm tseem ceeb hauv kev txhim kho thiab muaj kev nthuav dav ntawm T cov hlwb, uas yog qhov tseem ceeb rau kev tiv thaiv kev tiv thaiv. Tib neeg IL - 2 kuaj xyuas cov khoom siv yog qhov tseem ceeb hauv kev tshawb xyuas T Cell Teb, muab cov kev pom hauv cov kab mob tsis muaj zog. Qhov no tau cuam tshuam tsis tsuas yog rau kev tshawb fawb yooj yim tab sis kuj rau kev txhim kho kev kho mob rau kev tiv thaiv kab mob - Cov teeb meem cuam tshuam ntsig txog kev tiv thaiv kab mob.
● ● Cov neeg mob cancer immunotherapy thiab kev tiv thaiv kabmob
Lub luag haujlwm ntawm IL - 2 hauv kev txhawb nqa kev tiv thaiv kab mob qog nqaij hlav, tshwj xeeb hauv kev kho mob lub cev muaj peev xwm ntaus cov qog. Tib neeg IL - 2 kuaj pom yog qhov tseem ceeb hauv cov chaw tiv thaiv thiab kho cov lus teb, yog li ua kom zoo ntawm kev kho mob.
Txheej txheem cej luam ntawm IL - 2 Kuaj Cov Khoom Siv
● Elispot Cov khoom siv: rhiab heev thiab vib this
Elispot (enzyme - Txuas immunospot) txuas ntxiv rau lawv cov rhiab, muaj peev xwm ntawm kev ntes cytokines ntawm ib qho - cell qib. Tib neeg IL - 2 Elispot Cov khoom siv tau muaj txiaj ntsig tshwj xeeb hauv kev txheeb xyuas IL - 2 daim ntawv teev npe pub rau cov pejxeem, muab cov tshuaj ntsuam xyuas thiab cov lus pom zoo ntawm kev tiv thaiv kab mob. Qhov kev kub siab siab no yog qhov tsis txaus ntseeg hauv kev tshawb fawb txhaj tshuaj, tshwj xeeb tshaj yog thaum kuaj cov lus teb tsawg dua - Qib Cytokine hauv ntau qhov chaw.
● Elisa cov khoom siv: kev ntsuas cov tswv yim assay
Elisa cov khoom siv yog cov haujlwm ntawm cytokine nrhiav tau, muab ib txoj kev muaj zog thiab ncaj thiab ncaj qha il - 2 hauv ntau hom qauv. Tib neeg IL - 2 elisa cov khoom siv tau nyiam lawv txoj kev ntseeg tau thiab yooj yim ntawm kev siv, yooj yim ntawm kev siv tshuaj kho mob, kev txhaj tshuaj tiv thaiv kab mob, thiab kab mob pathogenesis.
Elispot Cov Khoom Siv Nta thiab Cov Nyiaj Pab
● Microplate - Cov kev ua yeeb yam
Cov hom micpplate ntawm Elispot cia siab tso cai rau High - Kev Ntsuam Xyuas Kev Tshawb Fawb, uas yog qhov zoo hauv kev kawm loj dua. Lub peev xwm los kuaj ntau cov hnoos qeev ib txhij zoo tshaj plaws thiab txo qhov hloov pauv, muab cov ntaub ntawv txhim khu kev qha ntau thoob plaws ntau txoj kev sim.
Zoo siab rau cov lus teb qis
Ib qho ntawm cov khoom siv standout ntawm Elispot cov khoom siv yog lawv lub peev xwm los kuaj xyuas cov kev tshawb fawb ntawm lub tshuaj tiv thaiv ntawm lub cev, ntau zaus tuaj yeem muaj cov kev cuam tshuam muaj txiaj ntsig zoo roj ntsha. Qhov kev nkag siab no ua rau Elispot cov cuab yeej tshawb fawb zoo rau kev txhaj tshuaj tiv thaiv thiab kev txhim kho kev tiv thaiv tshiab.
Elisa Cov Khoom Siv Hluav Taws Xob piav qhia
● Sandwich Elisa Txheej Txheem
Cov Sandwich Elisa yog cov qauv tseem ceeb ua haujlwm hauv tib neeg IL - 2 kuaj xyuas cov khoom siv vim nws qhov tshwj xeeb thiab rhiab. Cov txheej txheem no suav nrog thawj zaug los ntawm IL - 2 mus rau kev ntes cov tshuaj tiv thaiv kab mob, ua kom muaj cov tshuaj tua kab mob thib ob) sib txuam nrog cov IL - 2 lub siab.
● Kev ntsuas IL - 2 hauv ntshav thiab supernatants
Tib neeg IL - 2 elisa cov khoom siv tau siv los ntsuas cytekine theem hauv ntshav thiab kev coj noj coj ua ntawm tes supernatants. Lub peev xwm los tshawb xyuas cov IL - 2 lub siab rau kev nkag siab txog cov kab mob uas tsis sib haum xeeb thiab cov kev tshawb fawb hauv nroog, tshwj xeeb tshaj plaws hauv kev tshawb fawb txog cov kab mob thiab kev cuam tshuam kev cuam tshuam.
Colorimetric Elisa Nrhiav tau cov ntsiab lus
Kauj ruam - los yog - kauj ruam txheej txheem
Cov txheej txheem raug rau ib tus qauv Colorimetric cuam tshuam nrog cov kauj ruam pib nrog kev npaj coj mus, ua kom loj hlob nrog kev ntes cov tshuaj tiv thaiv kab mob. Tom qab ob peb ntxuav kom tshem tawm cov tshuaj tsis huv, qhov nrhiav kom tau cov tshuaj tiv thaiv kab mob antibody. Kauj ruam kawg yuav muaj kev sib ntxiv ntxiv uas cuam tshuam nrog cov enzyme los tsim cov xim xim hloov, muab cov ntaub ntawv ntau ntawm IL - 2 qib.
● Quamification hauv cell kab lis kev cai nruab nrab
Txawm li cas los xij ntawm cature friums muaj kev pom rau cytokine ntau lawm nyob rau hauv ntau sim kev sim. Qhov no yog pivotal hauv kev tshawb fawb tshawb xyuas cellular cov lus teb, qhov twg kev txiav txim siab cytokine ntau qib tuaj yeem pab tau ntawm Cell kev taw qhia txoj hauv kev thiab cov tshuaj cuam tshuam.
Qhov tseem ceeb ntawm qhov tseeb IL - 2 ntsuas
Kev Tshawb Fawb thiab Chaw Kho Mob
Yog IL - 2 ntsuas yog ua tiav rau ob qho kev tshawb fawb thiab chaw kuaj mob. Hauv kev tshawb fawb, nws ua rau cov cim tshwj xeeb ntawm cov lus teb tsis muaj zog, qhia kev txhim kho ntawm cov tshuaj tiv thaiv thiab cov tshuaj tiv thaiv. Clinically, IL - 2 theem tuaj yeem ua cov biomarkers rau cov kab mob los yog kho cov lus teb, pab hauv kev tswj hwm tus neeg mob thiab kho mob.
● Kev cuam tshuam rau kev kawm cytokine thiab kev kho
Lub peev xwm los ntsuas il - 2 muaj tseeb muaj qhov cuam tshuam rau cytokine kawm thiab kho. Nws pab txhawb kev ua hauj lwm tob tob txog cytokine tes hauj lwm thiab lawv txoj haujlwm ntawm kev txhim kho cytokine kho mob uas tuaj yeem hloov kho lub neej muaj txiaj ntsig zoo.
Cov kev sib tw hauv IL - 2 Nrhiav Kom Tau
● Sensitivity thiab Cov Teeb Meem Tshwj Xeeb
Ib qho ntawm thawj cov nyom hauv IL - 2 Kev tshawb nrhiav tau ua kom muaj kev paub txaus thiab tshwj xeeb. Kev sib txawv hauv kev ua haujlwm tshuaj tiv thaiv kev ua tau zoo tuaj yeem ua rau muaj qhov tseem ceeb ntawm kev xaiv tau txhim khu kev qha thiab cov khoom lag luam uas tau lees tias yog lawv cov khoom lag luam zoo.
● AdariaBility nyob rau hauv cov qauv coj
Lwm qhov kev sib tw tseem ceeb yog qhov txawv txav thaum ua piv txwv thiab kev npaj ua kom raug thiab rov ua dua tshiab ntawm cov txiaj ntsig. Cov qauv kev cai thiab kev cob qhia yog qhov tseem ceeb kom txo cov xwm txheej no, kom tau txais los ntawm tib neeg IL - 2 kuaj cov khoom siv yog cov khoom siv khov kho thiab ntseeg tau.
Yav tom ntej cov kev cia siab hauv IL - 2 Kuaj cov Khoom Siv
● Kev txhim kho Technological
Lub neej yav tom ntej ntawm tib neeg IL - 2 kuaj cov khoom siv tau cog lus, nrog kev nce qib thev naus laus zis txhim kho lawv cov rhiab, feem ntau, thiab yooj yim ntawm kev siv. Kev nrhav kev cai xws li ntau li ntau yam thiab taw tes - ntawm - Kev kuaj xyuas kev saib xyuas yog qhov kev soj ntsuam cov khoom siv ntawm cov khoom siv ntawm cov khoom siv no, ua rau lawv tau txais kev siv tau dua thiab siv tau zoo nyob rau ntau qhov chaw.
Muab cov ntawv thov dav thiab kev hloov tshiab
Raws li peb kev nkag siab ntawm IL - 2 Biology Nthuav, yog li ntawd ib yam nkaus yuav tau cov ntawv thov ntawm IL - 2 kuaj cov khoom siv. Kev Kho Mob Tshiab Cov tswv yim tsom rau IL - 2 txoj hauv kev nyob rau ntawm qab ntug kev mob, mob cancer, thiab dhau mus. Nruam nruam los ntawm tib neeg IL - 2 kuaj cov khoom siv tsim thiab cov chaw muag khoom yuav yog qhov tseem ceeb hauv kev xav tau cov kev xav tau no.
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Blukit los ntawm Jiangsu Hadgene, lub hauv paus hauv lub npe hauv Suzhou, yog lub npe coj hauv kev kho mob cellular innovoration. Nrog nws lub xeev - Ntawm - - Art GMP cov vaj tse GMP thiab R & D Chaw Tsim Khoom, Nws tau tsim cov chaw tsim khoom hla tebchaws thiab nthuav dav thoob plaws tebchaws. Hadgene tshwj xeeb hauv kev tsim cov platforms rau nucleic acid kev tsim kho, txhawb kev txhim kho ntawm lub tsheb - T, thiab qia cell. Cog lus rau kev kho mob cellular ntawm tes kho mob ntawm tes kho mob,Cov bluekityog qhov sib txuam nrog kev tswj kom zoo hauv kev tsim cov tshuaj cell. Lawv lub luag haujlwm tseem nthuav tawm hauv kev kho mob cellular cov khoom siv, tau txais txiaj ntsig cov neeg mob thoob ntiaj teb.
Lub Sijhawm Post: 2024 - 12 - 09 15:26:03
