Cov khoom siv BSA dab tsi?

Taw qhia rauCov Khoom Siv BSAs

Bovine Serum albumin (BSA) cov khoom siv yog qhov ua tau tsis muaj nqis hauv kev muaj roj ntsha hauv roj ntsha thiab biochemical lub chaw soj ntsuam. Cov khoom siv no yog tsim los pab cov kws tshawb nrhiav thiab kuaj cov kws kho mob, suav nrog cov tshuaj ntsuam muaj proteification, enzyme - Txuas immunosorben tseem (Elisa), thiab lwm yam. Nkag siab rau cov tib yam suav nrog hauv cov khoom siv BSA yog qhov tseem ceeb rau kev ua tiav ntawm kev sim thiab ua tiav cov txiaj ntsig tau.

Lub luag haujlwm ntawm BSA hauv kev sim

Qhov tseem ceeb ntawm BSA

BSA yog dav siv raws li tus qauv hauv cov protein kom muaj nuj nqis. Nws pabcuam ua tus neeg sawv cev ruaj khov rau cov enzymes thiab ua cov khoom lag luam protein ntau. Nws cov vagatility ua rau nws muaj feem tiv thaiv hauv cov asksays thiab kuaj mob.

BSA hauv Protein ua yeeb yam

Hauv cov protein ua haujlwm, BSA feem ntau siv los ua kev siv los sib piv cov protein ntau. Nws pab hauv kev ntsuas hluav taws xob, kom ntseeg tau tias kev sim ua kom tau txais txiaj ntsig thiab cov txiaj ntsig tau zoo.

Cheebtsam ntawm cov khoom siv BSA raug

Tseem muaj feem

• BSA Txuj Ci:Cov khoom siv lub ntsiab tseem ceeb, siv rau kev tsim cov kab mob nkhaus.
• Cov kev daws teeb meem tsis muaj:Qhov tseem ceeb rau kev tswj hwm lub pH thiab kev ruaj ntseg ntawm cov qauv.

Txheej txheem ntxiv

• Pipettes:Siv rau kev ntsuas yog thiab hloov cov kua.
• Test Tawv:Muaj cov qauv thiab reagents rau cov tshuaj tiv thaiv.

Cov kev daws teeb meem hauv BSA cov khoom siv

Lub luag haujlwm ntawm buffers

Buffers ua si lub luag haujlwm tseem ceeb hauv kev tswj hwm cov qib PHIB tsim nyog rau kev coj ua tau zoo. Hauv BSA cov khoom siv, buffers ntseeg tau tias BSA thiab lwmyam reagents nyob ruaj khov thoob plaws hauv cov txheej txheem sim.

Cov tsis sib xws

Cov buffers nquag suav nrog cov tshuaj qab ntsev suav nrog cov ntsev (PBS)

Kuaj cov hlab thiab pipettes

Siv cov leeg kuaj

Kev ntsuas cov leeg yog siv rau cov tshuaj tiv thaiv kev sib xyaw ua ke thiab feem ntau khij nrog cov kev ntsuas tiav rau kev ntsuas ua kua. Lawv tus qauv tsim tseem ua kom sib xyaw kom zoo ntawm cov tshuaj reagents.

Precision nrog pipettes

Pipettes yog qhov tseem ceeb rau qhov ua kom meej thiab tshem dej. Lawv qhov tseeb yog qhov tseem ceeb rau kev tswj hwm kev ua kom muaj txiaj ntsig ntawm kev sim.

Reagents thiab lawv siv

Cov tshuaj reagents

Reagents xws li dyes lossis chromogenic substrates feem ntau suav nrog rau kev pom ntawm kev pom. Cov reagents sib cuam tshuam nrog BSA, tsim cov xim xim uas cuam tshuam nrog cov protein ntau.

Reagent tuav

Kev ua kom zoo thiab cia ntawm cov tshuaj reagegents yog qhov tseem ceeb heev los xyuas kom meej lawv txoj kev ua tau zoo. Yam xws li lub teeb rhiab los yog qhov kub muaj kev ruaj ntseg yuav tsum txiav txim siab.

Kev Tuav thiab Qhia Qhia

Qhov tseem ceeb ntawm phau ntawv

Ib qho kev qhia ntawm phau ntawv qhia feem ntau suav nrog BSA cov khoom siv los coj cov neeg siv los ntawm kev sim teeb tsa, kom txhua kauj ruam yog ua raws li kev siv tau.

Kauj ruam - los ntawm - theem cov txheej txheem

Cov phau ntawv qhia no feem ntau muab kauj ruam - los ntawm - Cov txheej txheem teeb meem, daws cov lus qhia, thiab cov lus piav qhia ntawm cov qauv keeb kwm kev tshawb fawb txhua qhov kev sim.

Cia thiab Txij Nkawm Cheebtsam

Kev tsim nyog cia

BSA cov khoom siv feem ntau muaj cov lus qhia tshwj xeeb rau kev cia khoom, uas yuav suav nrog tub yees lossis tiv thaiv ntawm lub teeb, kom muaj kev ncaj ncees ntawm cov khoom siv cov khoom siv.

Cov Lus Qhia Txij Nkawm

Kev tu neeg tsis tu ncua, xws li cov pipette calibration, yog qhov tseem ceeb kom tsis txhob ntsuas kev ntsuas thiab ua kom muaj kev ntseeg tau ntawm kev sim ua kev sim.

Kev hloov pauv ntawm BSA cov khoom siv

Hom BSA Cov Khoom Siv

BSA cov khoom siv los ntawm ntau hom, txhua tus tsim rau cov ntawv thov tshwj xeeb, xws li cov qauv protein uas pom, lossis cov tshuaj tiv thaiv xov tooj ntawm tes. Cov khoom siv no tuaj yeem sib txawv raws li kev sib txuam ntawm BSA thiab suav nrog cov reagents.

Xaiv cov khoom siv tau zoo

Xaiv cov khoom siv BSA uas tsim nyog nyob ntawm cov kev npaj thov thiab cov cai tshwj xeeb ntawm kev sim, xws li rhiab thiab thev naus laus zis.

Xaus: Kos cov khoom siv BSA txoj cai BSA

Xaiv cov khoom siv BSA raug yog qhov tseem ceeb rau kev ua tiav ntawm koj txoj kev sim. Xav txog cov ntawv thov tshwj xeeb thiab cov kev tseev kom muaj xws li hom ntas tsis sib xws, reagent sib xyaw, thiab cov qauv kev ua kom pom zoo. Kev sib koom tes nrog cov chaw tsim khoom uas ntseeg tau, cov chaw muag khoom, lossis lub Hoobkas los xyuas kom meej thiab muaj txiaj ntsig ntawm koj cov khoom siv BSA.

Blukit muab cov kev daws teeb meem

Cov kab xiav, ib qho chaw xa khoom thiab cov chaw tsim khoom lag luam hauv daim teb biochemical, muaj cov khoom siv BSA cov khoom yuav tsum tau ua kom nruj. Peb cov khoom siv suav nrog cov qauv siab dua - cov qauv kev ua kom zoo, thiab txhua yam tsim nyog reagents, kom pom tseeb ntawm cov kev siv ntau yam. Tus khub nrog cov bluekit rau kev ua kom zoo, kev pab txhawb nqa, thiab kev tshaj lij kev txawj ntse, txawm tias muaj kev tso cai faib khoom. Xaiv cov xiav kom ua rau txhim kho qhov tseeb thiab kev ua haujlwm ntawm koj qhov kev sim ua haujlwm.