Lub hauv paus rau phau ntawv
Lub teb ntawm kev kho mob cell tau pom zoo tshaj li kaum xyoo dhau los, tshwj xeeb hauv kev nthuav dav thiab kev thov ntawm cov killer (NK). Cov kab mob tiv thaiv kab mob no, uas ua rau lub luag haujlwm tseem ceeb hauv lub cev tiv thaiv kev tiv thaiv lub cev, tau ua pivotal nyob rau hauv kev kho cov tshuaj ntsuab rau kev mob cancer thiab lwm yam kab mob. Cov lus qhia no rho tawm cov khoom siv ntawm nk ntawm trophobbast thiab engineer k562 lub hlwb, qhov tseem ceeb ntawm cytokines, thiab cov lus qhia yav tom ntej rau NK Cell kev kho mob. Tsis tas li ntawd, peb kov tau qhov tseem ceeb ntawmHost Cell DNA Cov Khoom Siv Nyiaj Ua NtejS nyob rau hauv kev ua kom zoo thiab kev nyab xeeb ntawm kev kho mob cell.
Txheej txheem cej luam ntawm NKE NK Cell nthuav tawm cov khoom siv
● Qhov tseem ceeb hauv kev kho mob cell
NK Cell cov khoom siv nthuav dav yog cov cuab yeej tseem ceeb hauv kev kho mob ntawm tes, ua rau lub amplification ntawm NK cell los kho cov qib. Cov khoom siv no pab txhawb nqa cov khoom siv kom txaus ntawm cov ntaub ntawv thov kom zoo rau cov ntawv thov kev kho mob, yog li txhim kho lawv lub peev xwm kho.
Cov khoom sib txawv ntawm cov khoom siv
Feem ntau, nk xov tooj ntawm tes nthuav dav muaj cov reagents uas suav nrog cov neeg noj haus nas, cytokines, thiab ib qho tshwj xeeb nk cell cell nruab nrab. Cov khoom siv no ua haujlwm Synergistically los tsim ib puag ncig zoo rau no thiab ua kom muaj kev ua kom muaj txiaj ntsig zoo thiab ua kom muaj kev coj dawb huv.
Lub luag haujlwm ntawm trophobbast hlwb hauv nk ua kom tau
● Mechanism ntawm kev txhawb nqa
TrophloBlast cells ib txwm sib cuam tshuam nrog uk hlwb thaum cev xeeb tub, ua rau muaj lub luag haujlwm tseem ceeb hauv kev hloov lawv txoj haujlwm. Nyob rau hauv cov ntsiab lus ntawm nk cell kev nthuav dav, trophobbast cells tuaj yeem txhawb nqa tutor -
● Cov txiaj ntsig ntawm kev siv lub hlwb trophoblast
Siv cov hlwb trophobbast hauv NK ntawm tes nthuav dav muab cov khoom siv tsis muaj zog ntxiv piv rau cov neeg txhais tau tias yog txhais tau tias yog khoom siv dag.
Engineered K562 Hle Hauv NK Cell Specansion
● CyTOKine qhia nyob rau hauv K562 hlwb
Engineered K562 cov hlwb yog nquag siv los ua cov neeg pub mis hauv NK ntawm tes nthuav dav. Lawv raug hloov kho los nthuav qhia cytokines xws li il - 15 thiab ir - 21, uas yog qhov tseem ceeb rau NK Cell kev loj hlob thiab ua kom muaj zog.
● Kev cuam tshuam ntawm irradiation thiab inactivation
Irradiation lossis inactivation ntawm K562 hlwb ua ntej lawv siv raws li kev tiv thaiv hlwb kom pom kev nyab xeeb los ntawm kev tiv thaiv lawv lub peev xwm los txhawb rau NCY cand ntawm tes.
Tseem ceeb ntawm cytokines nyob rau hauv nk ua kom tau
● Eil - 21 thiab nws cov teebmeem
Cytokines xws li IL - 21 yog qhov tseem ceeb rau CK kev ua haujlwm ntawm tes. IL - 21 Tsis tsuas yog txhawb Nk kev sib tw ntawm tes tawm tab sis kuj txhim kho lawv cov khoom siv cytoToxic, ua rau cov khoom siv ntawm NK.
● Synergistic signinging pathways
Cytokines ua haujlwm los ntawm ntau qhov chaw teeb tsa txoj hauv kev kom ua kom zoo nkauj. Nkag siab txog cov kab ke no yog qhov tseem ceeb rau kev ua kom zoo NY ntawm Cell Cell Kev nthuav dav thiab txhim kho lawv cov kev ua tau zoo.
NKIK CELARTES: Umbilical Qaum Versus veripal ntshav
Piv txwv ntawm tes tau tawm los
Umbilical qaum ntshav yog cov nplua nuj qhov chaw ntawm nkog hlwb, feem ntau yielding siab tshaj tus lej piv rau peripheral ntshav. Txawm li cas los xij, kev xaiv ntawm cov khoom no nyob ntawm qhov tshwj xeeb ntawm kev thov kho.
● Purity thiab qhov zoo ntawm kev nthuav dav nk hlwb
Ob qho chaw no tuaj yeem muab siab rau siab - zoo
Daim ntawv thov hauv tsheb - NK kev npaj xov tooj
Cov txheej txheem rau kev ua haujlwm lub tsheb ntev -
Tsheb - NK hlwb yog enjerved los qhia chimeric ovigen cov khoom plig, ua rau lawv cov mob hlwb tshwj xeeb. Kev npaj ntawm tsheb - NK hlwb koom nrog kev hloov caj ceg thiab nthuav dav, cov khoom siv uas tau yooj yim.
Zoo tshaj cov txheej txheem ib txwm muaj
Tsheb - NK hlwb muab ob peb qhov zoo dua cov txheej txheem qub, nrog rau kab mob sib txuas thiab muaj peev xwm los ua hom mob cancer muaj ntau yam ntawm cov mob cancer antigens.
Txheej txheem kev txhim kho rau txoj kev kho mob cell
Lub sijhawm thiab tus nqi ua haujlwm hauv kev txhim kho ntxov
Txhim kho cov txheej txheem ua tau zoo rau NK cell kev kho yog qhov tseem ceeb rau kev txo lub sijhawm thiab them tus nqi kev txhim kho theem thaum ntxov. Txhim kho cov kev cai thiab kev ua haujlwm tau npaj rau - Siv cov khoom siv nthuav dav tuaj yeem siv cov txheej txheem no ua kom tau txais txoj kev no.
● Cov tswv yim rau kev ua haujlwm ntawm cov khoom kho mob cell
Cov tswv yim xws li xws li kev siv tshuab, kev ua haujlwm kaw, thiab kev siv microbial yog qhov tseem ceeb rau kev ua tau zoo thiab muaj txiaj ntsig ntawm kev kho mob cell.
Txhim Kho Purity thiab Yield of NK Hlob
● Cov tswv yim los ua tiav siab
Cov tswv yim xws li ntws cytometry - Raws li kev cais tawm thiab sib nqus cov qib kev sib cais ua haujlwm kom tau txais cov ntawv thov kho mob.
● Cov kev sib tw thiab cov kev daws teeb meem hauv kev nthuav dav loj -
Scalering Up NK Cell Kev nthuav dav ua cov kev sib tw xws li tswj cov kev sib kis ntawm tes thiab ua haujlwm. Kev tsim kho tshiab Bioractor tsim thiab kev ntsuas xyuas cov tshuab txuas ntxiv yog cov kev daws teeb meem rau cov kev cov nyom no.
Cov txiaj ntsig ntawm kev siv NK Cell Basal Nruab Nrab
Role Lub luag haujlwm hauv kev nthuav dav ntawm tes thiab kev ua kom ua huaj
Nk xov tooj ntawm tes nruab nrab yog qhov tshwj xeeb los txhawb kev loj hlob thiab ua kom nkog hlwb, ua kom muaj kev ua tau zoo sib xws thoob plaws.
● Sib piv nrog ntau cytokines
Qhov nruab nrab qhov sib xyaw nrog ntau yam ntawm cytokines tso cai rau kev yoog raws li kev ua kom tau raws li kev xav tau tshwj xeeb.
Cov Lus Qhia Yav Tom Ntej rau NK Cell Kev Kho Mob
● Cov ncauj lus tawm hauv kev kho mob cell
Daim teb ntawm NK cell kev kho yog sai sai hloov zuj zus los ntawm kev txhim kho kom raug, txo qhov mob, thiab txhim kho qhov ntsuas ntawm kev kho mob.
Tej zaum muaj peev xwm nce qib hauv NK ntawm daim ntawv thov
Yav tom ntej kev nce qib tuaj yeem suav nrog kev txhim kho Universal Donor NK hlwb, thiab tus kheej NK cell kev kho mob rau tus neeg mob cov npe.
Ensuring zoo nrog tus tswv tsev ntawm tes DNA cov khoom siv nyiaj ua ntej
● Qhov tseem ceeb hauv kev kho mob cell
Host Cell DNA cov khoom siv nyiaj ua ntej yog qhov tseem ceeb hauv kev kho mob cell rau kev kuaj xyuas ntawm tes DNA. Cov khoom siv no ua kom muaj kev nyab xeeb thiab ua tau zoo ntawm kev kho mob cell kev kho mob los ntawm kev pheej hmoo ua paug.
Wrocess txoj haujlwm ntawm cov tuam ntxhab thiab cov chaw muag khoom
Cov khoom lag luam thiab lwm tus neeg uas muaj ntaub ntawv ua haujlwm ntawm tes DNA, xws liCov bluekit, Ua si lub luag haujlwm tseem ceeb hauv kev muab cov khoom siv tau txhim khu kev qha thiab siab - cov khoom zoo. Cov khoom siv no tseem ceeb heev rau kev saib xyuas kev ncaj ncees ntawm cov txheej txheem kev kho mob cell.
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Cov toj roob hauv pes ntawm kev kho mob cell yog txuas ntxiv hloov zuj zus, nrog cov leeg ntawm cov leeg ntawm qhov kev hloov kho no. Kev sib xyaw ua ke ntawm cov khoom siv nthuav dav kev nthuav dav, cytokine insights, thiab kev paub tseeb ntawm tes DNA cov khoom siv nyiaj ua ntej yog tsav kev txhim kho ntawm kev nyab xeeb ntawm kev nyab xeeb dua. Raws li kev lag luam tshais chaw rau pem hauv ntej, kev sib koom tes ntawm cov kws tshawb nrhiav, cov kws kho mob, thiab cov tuam ntxhab yuav muaj qhov tseem ceeb los ua kom muaj peev xwm them tag nrho ntawm NK Cell kev kho mob.
Jiangsu Hadgene, nyob rau hauv nws cov hom blukit®, sawv ntawm qhov ua ntej ntawm kev kho mob cellular innovation. Nrog nws lub tsev hauv paus hauv Suzhou thiab kev tsim chaw nyob thoob plaws Suav teb thiab North Carolina, Hadgene yog tho kev tsim cov kua qaub thiab kev kho mob cell. Cog lus rau kev tswj hwm zoo, Bukit® cov khoom lag luam kom muaj kev nyab xeeb thiab tcr - t, thiab qia cell - cov khoom lag luam. Nrog lub zeem muag ntawm kev tshoov siab cell kho kev kho mob cell kev tsim kho, toj siab tau mob siab rau kev txhim kho kev kho mob hauv ntiaj teb.
Lub Sijhawm Post: 2024 - 12 - 13:31:09
Tsheb - NK hlwb yog enjerved los qhia chimeric ovigen cov khoom plig, ua rau lawv cov mob hlwb tshwj xeeb. Kev npaj ntawm tsheb - NK hlwb koom nrog kev hloov caj ceg thiab nthuav dav, cov khoom siv uas tau yooj yim.
Zoo tshaj cov txheej txheem ib txwm muaj
Tsheb - NK hlwb muab ob peb qhov zoo dua cov txheej txheem qub, nrog rau kab mob sib txuas thiab muaj peev xwm los ua hom mob cancer muaj ntau yam ntawm cov mob cancer antigens.
Txheej txheem kev txhim kho rau txoj kev kho mob cell
Lub sijhawm thiab tus nqi ua haujlwm hauv kev txhim kho ntxov
Txhim kho cov txheej txheem ua tau zoo rau NK cell kev kho yog qhov tseem ceeb rau kev txo lub sijhawm thiab them tus nqi kev txhim kho theem thaum ntxov. Txhim kho cov kev cai thiab kev ua haujlwm tau npaj rau - Siv cov khoom siv nthuav dav tuaj yeem siv cov txheej txheem no ua kom tau txais txoj kev no.
● Cov tswv yim rau kev ua haujlwm ntawm cov khoom kho mob cell
Cov tswv yim xws li xws li kev siv tshuab, kev ua haujlwm kaw, thiab kev siv microbial yog qhov tseem ceeb rau kev ua tau zoo thiab muaj txiaj ntsig ntawm kev kho mob cell.
Txhim Kho Purity thiab Yield of NK Hlob
● Cov tswv yim los ua tiav siab
Cov tswv yim xws li ntws cytometry - Raws li kev cais tawm thiab sib nqus cov qib kev sib cais ua haujlwm kom tau txais cov ntawv thov kho mob.
● Cov kev sib tw thiab cov kev daws teeb meem hauv kev nthuav dav loj -
Scalering Up NK Cell Kev nthuav dav ua cov kev sib tw xws li tswj cov kev sib kis ntawm tes thiab ua haujlwm. Kev tsim kho tshiab Bioractor tsim thiab kev ntsuas xyuas cov tshuab txuas ntxiv yog cov kev daws teeb meem rau cov kev cov nyom no.
Cov txiaj ntsig ntawm kev siv NK Cell Basal Nruab Nrab
Role Lub luag haujlwm hauv kev nthuav dav ntawm tes thiab kev ua kom ua huaj
Nk xov tooj ntawm tes nruab nrab yog qhov tshwj xeeb los txhawb kev loj hlob thiab ua kom nkog hlwb, ua kom muaj kev ua tau zoo sib xws thoob plaws.
● Sib piv nrog ntau cytokines
Qhov nruab nrab qhov sib xyaw nrog ntau yam ntawm cytokines tso cai rau kev yoog raws li kev ua kom tau raws li kev xav tau tshwj xeeb.
Cov Lus Qhia Yav Tom Ntej rau NK Cell Kev Kho Mob
● Cov ncauj lus tawm hauv kev kho mob cell
Daim teb ntawm NK cell kev kho yog sai sai hloov zuj zus los ntawm kev txhim kho kom raug, txo qhov mob, thiab txhim kho qhov ntsuas ntawm kev kho mob.
Tej zaum muaj peev xwm nce qib hauv NK ntawm daim ntawv thov
Yav tom ntej kev nce qib tuaj yeem suav nrog kev txhim kho Universal Donor NK hlwb, thiab tus kheej NK cell kev kho mob rau tus neeg mob cov npe.
Ensuring zoo nrog tus tswv tsev ntawm tes DNA cov khoom siv nyiaj ua ntej
● Qhov tseem ceeb hauv kev kho mob cell
Host Cell DNA cov khoom siv nyiaj ua ntej yog qhov tseem ceeb hauv kev kho mob cell rau kev kuaj xyuas ntawm tes DNA. Cov khoom siv no ua kom muaj kev nyab xeeb thiab ua tau zoo ntawm kev kho mob cell kev kho mob los ntawm kev pheej hmoo ua paug.
Wrocess txoj haujlwm ntawm cov tuam ntxhab thiab cov chaw muag khoom
Cov khoom lag luam thiab lwm tus neeg uas muaj ntaub ntawv ua haujlwm ntawm tes DNA, xws liCov bluekit, Ua si lub luag haujlwm tseem ceeb hauv kev muab cov khoom siv tau txhim khu kev qha thiab siab - cov khoom zoo. Cov khoom siv no tseem ceeb heev rau kev saib xyuas kev ncaj ncees ntawm cov txheej txheem kev kho mob cell.
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Cov toj roob hauv pes ntawm kev kho mob cell yog txuas ntxiv hloov zuj zus, nrog cov leeg ntawm cov leeg ntawm qhov kev hloov kho no. Kev sib xyaw ua ke ntawm cov khoom siv nthuav dav kev nthuav dav, cytokine insights, thiab kev paub tseeb ntawm tes DNA cov khoom siv nyiaj ua ntej yog tsav kev txhim kho ntawm kev nyab xeeb ntawm kev nyab xeeb dua. Raws li kev lag luam tshais chaw rau pem hauv ntej, kev sib koom tes ntawm cov kws tshawb nrhiav, cov kws kho mob, thiab cov tuam ntxhab yuav muaj qhov tseem ceeb los ua kom muaj peev xwm them tag nrho ntawm NK Cell kev kho mob.
Jiangsu Hadgene, nyob rau hauv nws cov hom blukit®, sawv ntawm qhov ua ntej ntawm kev kho mob cellular innovation. Nrog nws lub tsev hauv paus hauv Suzhou thiab kev tsim chaw nyob thoob plaws Suav teb thiab North Carolina, Hadgene yog tho kev tsim cov kua qaub thiab kev kho mob cell. Cog lus rau kev tswj hwm zoo, Bukit® cov khoom lag luam kom muaj kev nyab xeeb thiab tcr - t, thiab qia cell - cov khoom lag luam. Nrog lub zeem muag ntawm kev tshoov siab cell kho kev kho mob cell kev tsim kho, toj siab tau mob siab rau kev txhim kho kev kho mob hauv ntiaj teb.
Lub Sijhawm Post: 2024 - 12 - 13:31:09
