NK Cell Cedansion
NK Cell Cedansion
✔ E mālama iā K562 Faild Faild CITELE (IL - 21+)
Prer - Irradiated, Cytokine - Hoʻonuiʻia no keʻano o ka hanaʻana o NK.
✔ serum - free nk expansion kit
GMP -'Odia nodia no ka hoʻokeleʻana o ka Stidical - Scale.
✔ High - Kaʻaila NK Cells (> 90% CD56 +)
Hōʻoiaʻia no nā kumu kokoʻelua a me nā cods koko.
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NA MEA:
Hoʻolālā no NK Cell a me ke kaʻa - NK Cell hoʻomākaukauʻana mai ke koko peripheral mai ke koko koko (pbmc) a me ke koko.Ke kaʻina hana hoʻomohala: EXCELERATRATRATRATRATRATRATRATER FILEATER - E HOOLOLO ANA I KA LOHE ANA MA NK / NK / NK CELL GRATER FORY FORY FORY FORY.
Nā kiko'ī hana
Hana Hana Kūʻai Kaoaohai NK CELL TRIFIT (%) > 90% CD3 + CAND CONEDALLUA (%) <1% ʻO ka pākuhiʻo NK Cell Repansion > 5,000 - pou Cell vill (%) > 80% Nā helu helu:
E like me ka hoʻonuiʻiaʻana o NK Cerols e like me Allogenec, Off - Na - Nā Kauwila Pūnaewele, nā pono kūpono a me ka maʻemaʻe. ʻO ka Blue Catr® NK Cell Cell Repansion System
• ʻO Robust a me ka hoʻonui houʻana - Ma Loaʻa iā> 10,000 - Pihi Nk Nk Motem Proplication i loko o 14 mau lā
• Hāʻawi - I - Kōkua Donor - Ma Mālama i nā helu expansion ma waena o nā mea hoʻonani likeʻole
• Nā mana kiʻekiʻe nink (hema hema)ʻAʻole maikaʻi loa, 14 - lā i hoʻonuiʻiaʻo NK Cells i ka hana Tumoricidal e like me ka cryopreservation a me ka pale.
T cell prity (pre - vs post - koho)
ʻO ka hana hoʻonuiʻana i ka hana:
Ke kiʻekiʻe - hoʻomaʻemaʻe Triights T cell reveration reagent a hoʻonuiʻia i loko serum - free t cell bastal medium,
Hōʻike:> 100 - Polokalamu Polokalamu (Lā 10 - 14),Kūlike loa> 90%
Mālamaʻia t - cell hana (CD4 + / CD8 + Ratio Prereervation)
ʻO ka hoʻonuiʻana o ka cell & vibility
CDRS CDRS DAND CHANDUCTIONS:
Ke kaʻa kaʻa kaʻa transducing, i ka wā e hui pū ai me ka Viral transducing B (Harral e - Hancer B),
Hōʻike:> 60% Kālepa + T mau mea e kahe cyptross 2 - 3
T cell transduction effuency
| Hana Hana Kūʻai | Kaoaohai |
| NK CELL TRIFIT (%) | > 90% |
| CD3 + CAND CONEDALLUA (%) | <1% |
| ʻO ka pākuhiʻo NK Cell Repansion | > 5,000 - pou |
| Cell vill (%) | > 80% |
ʻO kaʻike moku
Hāʻawi mākou i nā mea i hoʻokaʻawaleʻia ma nā kauoha a pau. ʻO ka maʻamau, e hiki mai kāu kauoha i loko o 5 - 7 mau lāʻoihana maʻAmelika Hui PūʻIa a ma loko o 10 mau lāʻoihana. Eia nō naʻe, eʻoluʻolu e hoʻomaopopo i ka laweʻana i nā wahi'āina e lōʻihi lōʻihi paha.
ʻO ka manawa hoʻouna: Ua hana pinepineʻia nā kauoha i loko o 1 - 3 mau lāʻoihana. I ka manawa i hoʻounaʻia ai kāu kauoha, e loaʻa iāʻoe kahi leka uila hōʻoia me kaʻikeʻike.
ʻO kaʻike nui
E kauoha i ke kauoha: Ma hope o ka ukuʻia o ke kauoha, pono kā mākou hale kūʻai aku i kekahi manawa e hana i kāu kauoha. E loaʻa iāʻoe kahi hoʻolaha i ka wā i hoʻounaʻia ai kāu kauoha.
Nā manawa hāʻawi: Ma ka hapa nui o nā hihia, e hāʻawiʻia ka pākeke i loko o ka manawa i kohoʻia. Eia nō naʻe,ʻo ka lā lawe maoli e pili ana i ka holoʻana e nā hoʻonohonoho o ka holo, nā kūlana o ka wā, a me nā mea'ē aʻe. E lōʻihi ka lōʻihi o ka hana o ka hāʻawiʻana ma mua o nā kauoha no nā kauoha e komo i nā mea ma mua a iʻole nā mea maʻamau. Eʻoluʻolu e kiʻi i kaʻikeʻike no ka lā hoʻokele nui loa.
Nā pilikia hoʻouna: Ināʻikeʻoeʻaʻole i hāʻawiʻia kāu pākeke i loko o ka manawa i'ōleloʻia; Hōʻike kaʻikeʻikepili i hōʻikeʻia ua hāʻawiʻia ka pākeke akāʻaʻoleʻoe i loaʻa; a iʻole kāu pākeke e pili ana i nā mea i hala a iʻole nā mea kūponoʻole a iʻole nā pilikia logistics, eʻoluʻolu e hoʻopili i kā mākou lawelawe kūʻai aku i loko o 7 mau lā e hiki ai iā mākou ke helu koke i kēia mau pilikia.
Kauoha i ka huliʻana
Ke hoʻounaʻia nei kāu kauoha, e loaʻa iāʻoe kahi leka uila me kahi helu helu a me kahi loulou e nānā i kāu hoʻouna.
Hiki iāʻoe ke nānā pololei i kāu kauoha ma kā mākou pūnaewele ma o ka hoʻopaʻaʻana i kāu moʻokāki a nānā i kāu mōʻaukala kauoha.
Nā palena hoʻouna
Eʻoluʻolu e hoʻopiha i ka leka uila i ka kiko'ī Ināʻaʻole, makemake mākou e hoʻohana iā EMS no ka hāʻawiʻana (ʻoi aku ka lohi ma mua o nā mea'ē aʻe, e lawe ana i kahi 1 - 2 mau mahina a lōʻihi paha).
Nā hana maʻamau a me nā kulekeleʻauhau
E hoʻomaopopo eʻaeʻia kekahi mau hana maʻamau,ʻauhau, a iʻole nā uku hoʻolimalima a i hoʻopuka i ka wā o ka hoʻounaʻana i ka mea kūʻai. ʻO kēia mau uku e hāʻawiʻia ma muli o ka'āina kahi i hāʻawiʻia a hoʻoholoʻia e nā luna maʻamau o nā kūloko.
Ma ke kūʻaiʻana mai i kā mākou pūnaewele, eʻaeʻoe e uku i nā hana a iʻole nāʻauhau e pili ana i kāu kauoha. ʻAʻole mākou e kuleana no nā delays i hoʻokumuʻia e nā hoʻomaʻemaʻe maʻamau.
Nā kulekele koho koho
Ke hōʻea nei kāu kauoha i ka wahi koho i kohoʻia a iʻole ka hāʻawiʻana i kahi wahi, eʻoluʻolu e hōʻoia i ka hōʻiliʻili wikiwiki. Ināʻaʻole i kiʻiʻia ka uku i loko o ka manawa i kohoʻia, e hoʻouna mākou i kahi hoʻomanaʻo ma o ka leka uila a iʻole SMS. Eia naʻe, ināʻaʻole i hōʻiliʻiliʻia ka pākaukau i loko o ka manawa i kohoʻia, a loaʻa paha kekahi mau maʻi a iʻole ka maʻi e like me ka hopena, e mālamaʻia ka mea kūʻai aku. Mahalo nui mākou iāʻoe e hōʻiliʻili i kāu uku wikiwiki e pale i nā pilikia kūpono.
MEA KAHAKAU: E like me kā mākou huahana i lalo o kaʻano kūikawā
