Lentivirus titer p24 rapid elissa usic
Lentivirus titer p24 rapid elissa usic
✔ Correlates me Tu / ML (1 ng p24 × 1 × 104-105 Ke weheʻana i nā'āpana)
✔ 21 CFR mahele 11 - Mākaukau Hōʻikeʻia nā'ōlelo hōʻike uila uila
✔ SPIKE HECE 92 - 108% I loko o nā pepa lv
Kailima:
Ka pahu pahu e lawelawe ai Sandwich Elisa Methoddology no HIV - 1 P24 Ka helu:
Microplate i uhiʻia me anti - p24 monoclolonal antibody
Nā Kūlana Antiboy - Antigen - Antigen -ʻO kaʻike antibody paʻakikī i ka wā o ka incbation
Ua hanaʻo TMB substrate i nā mea i hoʻopaʻaʻia i ka hōʻailona hōʻailona ma P24
Nā Kūlana Kūlana:
|
Pākaukau |
Kaoaohai |
|
Moni hanana |
1.37-1000 NG / ML |
|
Sentivices (Lod) |
0.35 NG / ML |
|
'Clelo pololei |
Intra - waiwai cv <10% |
|
Pololei |
85-115% hoʻihoʻi |
Nā hiʻohiʻona nui:
Rapid 45 - min Protocol (RT Incubation)
Mākaukau - I - E hoʻohana i nā uku
ʻO Correlates me ka terer viral (1 ng p24 × 10 <4 3 <3 <(sup> -10 tu)
Nā Kānāwai Kūlana:
Datistateat:
He aha ka pahu ma ka pahu?
| Hui | Kaoaohai | Kūlana paʻa |
| Ua kohoʻia he Palapala | 1 × 96 96! Aloha | ʻOihana lumi |
| Anti - Huv - 1 p24 - huehue conjugate | 6 ml | 2 - 2 ° C |
| HIV - 1 P24 hoʻonohonoho hoʻonohonoho hoʻonohonoho | S0 - S7 (7 mau kūlana +ʻaʻole) | 2 - 2 ° C |
| ʻO ka wahine o ka virus | 6 ml | 2 - 2 ° C |
| Sample dilisent buffer | 50 ml | ʻOihana lumi |
| 10 × pbst Fore Curffer | 50 ml | ʻOihana lumi |
| Tmb substrate | 12 ML | 2 - 2 ° C |
| Hooki i ka hopena | 12 ML | ʻOihana lumi |
| Papa lole paalei | 5 | ʻOihana lumi |
| NA PALANE palapala | 1 | N / a |
Kaoaohai
|
Antshay rov |
1.37 - 1000 ng / ml |
|
ʻIke maka |
0.35 NG / ML |
|
'Clelo pololei |
CV% ≤ 10%, re% ≤ ± 15% |
ʻO kaʻike moku
Hāʻawi mākou i nā mea i hoʻokaʻawaleʻia ma nā kauoha a pau. ʻO ka maʻamau, e hiki mai kāu kauoha i loko o 5 - 7 mau lāʻoihana maʻAmelika Hui PūʻIa a ma loko o 10 mau lāʻoihana. Eia nō naʻe, eʻoluʻolu e hoʻomaopopo i ka laweʻana i nā wahi'āina e lōʻihi lōʻihi paha.
ʻO ka manawa hoʻouna: Ua hana pinepineʻia nā kauoha i loko o 1 - 3 mau lāʻoihana. I ka manawa i hoʻounaʻia ai kāu kauoha, e loaʻa iāʻoe kahi leka uila hōʻoia me kaʻikeʻike.
ʻO kaʻike nui
E kauoha i ke kauoha: Ma hope o ka ukuʻia o ke kauoha, pono kā mākou hale kūʻai aku i kekahi manawa e hana i kāu kauoha. E loaʻa iāʻoe kahi hoʻolaha i ka wā i hoʻounaʻia ai kāu kauoha.
Nā manawa hāʻawi: Ma ka hapa nui o nā hihia, e hāʻawiʻia ka pākeke i loko o ka manawa i kohoʻia. Eia nō naʻe,ʻo ka lā lawe maoli e pili ana i ka holoʻana e nā hoʻonohonoho o ka holo, nā kūlana o ka wā, a me nā mea'ē aʻe. E lōʻihi ka lōʻihi o ka hana o ka hāʻawiʻana ma mua o nā kauoha no nā kauoha e komo i nā mea ma mua a iʻole nā mea maʻamau. Eʻoluʻolu e kiʻi i kaʻikeʻike no ka lā hoʻokele nui loa.
Nā pilikia hoʻouna: Ināʻikeʻoeʻaʻole i hāʻawiʻia kāu pākeke i loko o ka manawa i'ōleloʻia; Hōʻike kaʻikeʻikepili i hōʻikeʻia ua hāʻawiʻia ka pākeke akāʻaʻoleʻoe i loaʻa; a iʻole kāu pākeke e pili ana i nā mea i hala a iʻole nā mea kūponoʻole a iʻole nā pilikia logistics, eʻoluʻolu e hoʻopili i kā mākou lawelawe kūʻai aku i loko o 7 mau lā e hiki ai iā mākou ke helu koke i kēia mau pilikia.
Kauoha i ka huliʻana
Ke hoʻounaʻia nei kāu kauoha, e loaʻa iāʻoe kahi leka uila me kahi helu helu a me kahi loulou e nānā i kāu hoʻouna.
Hiki iāʻoe ke nānā pololei i kāu kauoha ma kā mākou pūnaewele ma o ka hoʻopaʻaʻana i kāu moʻokāki a nānā i kāu mōʻaukala kauoha.
Nā palena hoʻouna
Eʻoluʻolu e hoʻopiha i ka leka uila i ka kiko'ī Ināʻaʻole, makemake mākou e hoʻohana iā EMS no ka hāʻawiʻana (ʻoi aku ka lohi ma mua o nā mea'ē aʻe, e lawe ana i kahi 1 - 2 mau mahina a lōʻihi paha).
Nā hana maʻamau a me nā kulekeleʻauhau
E hoʻomaopopo eʻaeʻia kekahi mau hana maʻamau,ʻauhau, a iʻole nā uku hoʻolimalima a i hoʻopuka i ka wā o ka hoʻounaʻana i ka mea kūʻai. ʻO kēia mau uku e hāʻawiʻia ma muli o ka'āina kahi i hāʻawiʻia a hoʻoholoʻia e nā luna maʻamau o nā kūloko.
Ma ke kūʻaiʻana mai i kā mākou pūnaewele, eʻaeʻoe e uku i nā hana a iʻole nāʻauhau e pili ana i kāu kauoha. ʻAʻole mākou e kuleana no nā delays i hoʻokumuʻia e nā hoʻomaʻemaʻe maʻamau.
Nā kulekele koho koho
Ke hōʻea nei kāu kauoha i ka wahi koho i kohoʻia a iʻole ka hāʻawiʻana i kahi wahi, eʻoluʻolu e hōʻoia i ka hōʻiliʻili wikiwiki. Ināʻaʻole i kiʻiʻia ka uku i loko o ka manawa i kohoʻia, e hoʻouna mākou i kahi hoʻomanaʻo ma o ka leka uila a iʻole SMS. Eia naʻe, ināʻaʻole i hōʻiliʻiliʻia ka pākaukau i loko o ka manawa i kohoʻia, a loaʻa paha kekahi mau maʻi a iʻole ka maʻi e like me ka hopena, e mālamaʻia ka mea kūʻai aku. Mahalo nui mākou iāʻoe e hōʻiliʻili i kāu uku wikiwiki e pale i nā pilikia kūpono.
MEA KAHAKAU: E like me kā mākou huahana i lalo o kaʻano kūikawā
