ʻO Hek293 Nā Kūlana Kūʻai Kūʻai DNA i ka nānāʻana i ka pahu nānā (QPCr)
ʻO Hek293 Nā Kūlana Kūʻai Kūʻai DNA i ka nānāʻana i ka pahu nānā (QPCr)
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✔Triplex QPCR i helu 99/200/307 BP FRERSE.
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✔ Nist - Nā Kūlana Kūʻai Kūʻai e hōʻoia i ka pololei metrollogical pololei.
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✔ ≤15% cv e hālāwai me nā koi gmp.
Nā Kūlana DNA (> 99bp)ʻike
ʻO ka nohoʻana o ke koena DNA (> 200BP)ʻike
ʻO ka nohoʻana o ke koena DNA (> 3077BP)ʻike
| ʻAʻole. | Nā'āpana | Hoʻopiha i nā helu helu / Vial | Ka nui | Nā kūlana mālama |
| 1 | Hek293 Kauka a Pomi Pox - 99 | 300 μL | 1 | - 18 ° C, paleʻia mai ka mālamalama |
| 2 | Henk293 phiper & probe moko - 200 | 300 μL | 1 | - 18 ° C, paleʻia mai ka mālamalama |
| 3 | Henk293 phiper & probe manual - 307 | 300 μL | 1 | - 18 ° C, paleʻia mai ka mālamalama |
| 4 | Hek293 DNA helu helu | 60 μl | 1 | - 11 ° C |
| 5 | 2 × Hoʻomaopopo iā QPCR Joel Mill | 1.25 ml | 4 | - 11 ° C |
| 6 | DNA DILIELE | 1 ml | 4 | - 11 ° C |
| 7 | ʻO iPC Mix (Pūnaewele maikaʻi maikaʻi) | 450 μl | 1 | - 18 ° C, paleʻia mai ka mālamalama |
| 8 | ROX High * (ENTER DYE) | 200 μl | 1 | - 18 ° C, paleʻia mai ka mālamalama |
| 9 | ROX haʻahaʻa * (ENTER DYE) | 200 μl | 1 | - 18 ° C, paleʻia mai ka mālamalama |
Kaoaohai
| Pākaukau | Kaoaohai |
| Ka helu helu | Ka HG - HL002 |
| Antshay rov | 3.00 × 10 6 épingles 3.00 × 10⁶ FG / μl |
| Ka palena o ka ike | 3.00 fg / μl |
| Palena palena | 8 FG / μl |
| Kit nui | Nā hopena 300 |
| Ana wela ana | - 20 ° C |
| 'Clelo pololei | Cv% ≤ 15% |
ʻO kaʻike moku
Hāʻawi mākou i nā mea i hoʻokaʻawaleʻia ma nā kauoha a pau. ʻO ka maʻamau, e hiki mai kāu kauoha i loko o 5 - 7 mau lāʻoihana maʻAmelika Hui PūʻIa a ma loko o 10 mau lāʻoihana. Eia nō naʻe, eʻoluʻolu e hoʻomaopopo i ka laweʻana i nā wahi'āina e lōʻihi lōʻihi paha.
ʻO ka manawa hoʻouna: Ua hana pinepineʻia nā kauoha i loko o 1 - 3 mau lāʻoihana. I ka manawa i hoʻounaʻia ai kāu kauoha, e loaʻa iāʻoe kahi leka uila hōʻoia me kaʻikeʻike.
ʻO kaʻike nui
E kauoha i ke kauoha: Ma hope o ka ukuʻia o ke kauoha, pono kā mākou hale kūʻai aku i kekahi manawa e hana i kāu kauoha. E loaʻa iāʻoe kahi hoʻolaha i ka wā i hoʻounaʻia ai kāu kauoha.
Nā manawa hāʻawi: Ma ka hapa nui o nā hihia, e hāʻawiʻia ka pākeke i loko o ka manawa i kohoʻia. Eia nō naʻe,ʻo ka lā lawe maoli e pili ana i ka holoʻana e nā hoʻonohonoho o ka holo, nā kūlana o ka wā, a me nā mea'ē aʻe. E lōʻihi ka lōʻihi o ka hana o ka hāʻawiʻana ma mua o nā kauoha no nā kauoha e komo i nā mea ma mua a iʻole nā mea maʻamau. Eʻoluʻolu e kiʻi i kaʻikeʻike no ka lā hoʻokele nui loa.
Nā pilikia hoʻouna: Ināʻikeʻoeʻaʻole i hāʻawiʻia kāu pākeke i loko o ka manawa i'ōleloʻia; Hōʻike kaʻikeʻikepili i hōʻikeʻia ua hāʻawiʻia ka pākeke akāʻaʻoleʻoe i loaʻa; a iʻole kāu pākeke e pili ana i nā mea i hala a iʻole nā mea kūponoʻole a iʻole nā pilikia logistics, eʻoluʻolu e hoʻopili i kā mākou lawelawe kūʻai aku i loko o 7 mau lā e hiki ai iā mākou ke helu koke i kēia mau pilikia.
Kauoha i ka huliʻana
Ke hoʻounaʻia nei kāu kauoha, e loaʻa iāʻoe kahi leka uila me kahi helu helu a me kahi loulou e nānā i kāu hoʻouna.
Hiki iāʻoe ke nānā pololei i kāu kauoha ma kā mākou pūnaewele ma o ka hoʻopaʻaʻana i kāu moʻokāki a nānā i kāu mōʻaukala kauoha.
Nā palena hoʻouna
Eʻoluʻolu e hoʻopiha i ka leka uila i ka kiko'ī Ināʻaʻole, makemake mākou e hoʻohana iā EMS no ka hāʻawiʻana (ʻoi aku ka lohi ma mua o nā mea'ē aʻe, e lawe ana i kahi 1 - 2 mau mahina a lōʻihi paha).
Nā hana maʻamau a me nā kulekeleʻauhau
E hoʻomaopopo eʻaeʻia kekahi mau hana maʻamau,ʻauhau, a iʻole nā uku hoʻolimalima a i hoʻopuka i ka wā o ka hoʻounaʻana i ka mea kūʻai. ʻO kēia mau uku e hāʻawiʻia ma muli o ka'āina kahi i hāʻawiʻia a hoʻoholoʻia e nā luna maʻamau o nā kūloko.
Ma ke kūʻaiʻana mai i kā mākou pūnaewele, eʻaeʻoe e uku i nā hana a iʻole nāʻauhau e pili ana i kāu kauoha. ʻAʻole mākou e kuleana no nā delays i hoʻokumuʻia e nā hoʻomaʻemaʻe maʻamau.
Nā kulekele koho koho
Ke hōʻea nei kāu kauoha i ka wahi koho i kohoʻia a iʻole ka hāʻawiʻana i kahi wahi, eʻoluʻolu e hōʻoia i ka hōʻiliʻili wikiwiki. Ināʻaʻole i kiʻiʻia ka uku i loko o ka manawa i kohoʻia, e hoʻouna mākou i kahi hoʻomanaʻo ma o ka leka uila a iʻole SMS. Eia naʻe, ināʻaʻole i hōʻiliʻiliʻia ka pākaukau i loko o ka manawa i kohoʻia, a loaʻa paha kekahi mau maʻi a iʻole ka maʻi e like me ka hopena, e mālamaʻia ka mea kūʻai aku. Mahalo nui mākou iāʻoe e hōʻiliʻili i kāu uku wikiwiki e pale i nā pilikia kūpono.
MEA KAHAKAU: E like me kā mākou huahana i lalo o kaʻano kūikawā
