ʻO CytotOxity Cytotoxic istay Kit (hoʻokuʻu i nā'āpana kiko'ī)
ʻO CytotOxity Cytotoxic istay Kit (hoʻokuʻu i nā'āpana kiko'ī)
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✔DULOT - LABEL SDYTOXICITICE (CFSE + 7 - AAD)
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✔Pittimized no ka hoʻokuʻuʻana i nā laina keleponaʻo subpension
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✔ Mactidadated me nā kaʻa - T / NK Cell Therapies
Kailima:
'Ōlelo Suspedion target cell cell gettotika cit ua hoʻolālāʻia e ana i ke kikowaena o ka cell support - Mediated cytotopicity e kū'ē i ka suspension - nā mea i hoʻopaʻaʻia. Aia i ka pahu:
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Cfsee (Carboxyfluorein succinamidyl succinimidyl.
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7 - Aad (7 - aminocoackinmycin d)
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Moʻomeheu moʻomeheu a &Assay Buffer
Noi
✔ʻO ka loiloi hana hana o nā cells infune preful (NK Celells, ke kaʻa - NK, CAR - T, TCR - T, TM Comels) E kū'ē i nā laina keleponaʻo subpension
✔ Hoʻolālā Hoʻolaha: E koho i nā mea hana hana cell i nā hana hana
✔ Honua mālamalama: E hoʻokuʻu i nā hoʻokolohua hoʻokolohua a me nā hana hoʻokūkū no ka Clinical - Nā huahana papa
Nā hiʻohiʻona nui
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Dual -'ōnaehana hana: CFSE (Nā Kūlana Live) + 7 - AAD (EAD CHELS (EMIKI) No ka helu helu CytotOTiction pololei
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Gm -: Hōʻoiaʻia no ka hoʻomohalaʻana o ka cell i ka hoʻomohalaʻana ma lalo o FDA / EMA alakaʻi
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Workfolflow - mākaukau: Pre - preptimized protocols no ka hoʻopiliʻana i ka hoʻohuiʻana
Nā Hoʻolālā Nui Keʻokeʻo
▸ ʻO ka cell cell cell: Kūpono me nā laina tumor maʻamau (e.g., k562, Raji)
▸ Hoʻololi hoʻoponopono: Kākoʻo i loko - Nā haʻawina e pili ana i nā helu palapala
▸ Hana holomua: ≤10% cv in inter - waiwai pololei
Datistateat:
E kaomiʻana i ka loiloi cytometry o Nk Cell - Mediated Cytotoxicity e kū'ē iā CFSE - Labeled K562
NK Center Cytotoxicy pepehi iā Curve e kū'ē iā CFSE - Ua mālamaʻiaʻo K562 mau kāleka
| Id | Hui | Ka nui | Nā kūlana mālama | Wahi nani |
|---|---|---|---|---|
| Hg - MD001 | Medium a me | 100 ml | 2 - 2 ° C | Kit i |
| Hg - fw001 | Assay Buffer | 100 ml | 2 - 2 ° C | - |
| Hg - Cfse | Cfse stain | 1, vial | - 20 ° C | Kuku ii |
| Hg - 7 - aad | 7 - AAD Stain | 1, vial | - 20 ° C | - |
Kuhi i ka Hoʻohālike
| Hana Hana Kūʻai | Kaʻaeʻana |
| Pololei | Hoʻopau iā 70 - 130% |
| Ōkahi'ā | Bias 70 - 130% |
| Lawehala | CV ≤ 10% |
| Koho UIANTIONCIANTION | CV ≤ 10% |
ʻO kaʻike moku
Hāʻawi mākou i nā mea i hoʻokaʻawaleʻia ma nā kauoha a pau. ʻO ka maʻamau, e hiki mai kāu kauoha i loko o 5 - 7 mau lāʻoihana maʻAmelika Hui PūʻIa a ma loko o 10 mau lāʻoihana. Eia nō naʻe, eʻoluʻolu e hoʻomaopopo i ka laweʻana i nā wahi'āina e lōʻihi lōʻihi paha.
ʻO ka manawa hoʻouna: Ua hana pinepineʻia nā kauoha i loko o 1 - 3 mau lāʻoihana. I ka manawa i hoʻounaʻia ai kāu kauoha, e loaʻa iāʻoe kahi leka uila hōʻoia me kaʻikeʻike.
ʻO kaʻike nui
E kauoha i ke kauoha: Ma hope o ka ukuʻia o ke kauoha, pono kā mākou hale kūʻai aku i kekahi manawa e hana i kāu kauoha. E loaʻa iāʻoe kahi hoʻolaha i ka wā i hoʻounaʻia ai kāu kauoha.
Nā manawa hāʻawi: Ma ka hapa nui o nā hihia, e hāʻawiʻia ka pākeke i loko o ka manawa i kohoʻia. Eia nō naʻe,ʻo ka lā lawe maoli e pili ana i ka holoʻana e nā hoʻonohonoho o ka holo, nā kūlana o ka wā, a me nā mea'ē aʻe. E lōʻihi ka lōʻihi o ka hana o ka hāʻawiʻana ma mua o nā kauoha no nā kauoha e komo i nā mea ma mua a iʻole nā mea maʻamau. Eʻoluʻolu e kiʻi i kaʻikeʻike no ka lā hoʻokele nui loa.
Nā pilikia hoʻouna: Ināʻikeʻoeʻaʻole i hāʻawiʻia kāu pākeke i loko o ka manawa i'ōleloʻia; Hōʻike kaʻikeʻikepili i hōʻikeʻia ua hāʻawiʻia ka pākeke akāʻaʻoleʻoe i loaʻa; a iʻole kāu pākeke e pili ana i nā mea i hala a iʻole nā mea kūponoʻole a iʻole nā pilikia logistics, eʻoluʻolu e hoʻopili i kā mākou lawelawe kūʻai aku i loko o 7 mau lā e hiki ai iā mākou ke helu koke i kēia mau pilikia.
Kauoha i ka huliʻana
Ke hoʻounaʻia nei kāu kauoha, e loaʻa iāʻoe kahi leka uila me kahi helu helu a me kahi loulou e nānā i kāu hoʻouna.
Hiki iāʻoe ke nānā pololei i kāu kauoha ma kā mākou pūnaewele ma o ka hoʻopaʻaʻana i kāu moʻokāki a nānā i kāu mōʻaukala kauoha.
Nā palena hoʻouna
Eʻoluʻolu e hoʻopiha i ka leka uila i ka kiko'ī Ināʻaʻole, makemake mākou e hoʻohana iā EMS no ka hāʻawiʻana (ʻoi aku ka lohi ma mua o nā mea'ē aʻe, e lawe ana i kahi 1 - 2 mau mahina a lōʻihi paha).
Nā hana maʻamau a me nā kulekeleʻauhau
E hoʻomaopopo eʻaeʻia kekahi mau hana maʻamau,ʻauhau, a iʻole nā uku hoʻolimalima a i hoʻopuka i ka wā o ka hoʻounaʻana i ka mea kūʻai. ʻO kēia mau uku e hāʻawiʻia ma muli o ka'āina kahi i hāʻawiʻia a hoʻoholoʻia e nā luna maʻamau o nā kūloko.
Ma ke kūʻaiʻana mai i kā mākou pūnaewele, eʻaeʻoe e uku i nā hana a iʻole nāʻauhau e pili ana i kāu kauoha. ʻAʻole mākou e kuleana no nā delays i hoʻokumuʻia e nā hoʻomaʻemaʻe maʻamau.
Nā kulekele koho koho
Ke hōʻea nei kāu kauoha i ka wahi koho i kohoʻia a iʻole ka hāʻawiʻana i kahi wahi, eʻoluʻolu e hōʻoia i ka hōʻiliʻili wikiwiki. Ināʻaʻole i kiʻiʻia ka uku i loko o ka manawa i kohoʻia, e hoʻouna mākou i kahi hoʻomanaʻo ma o ka leka uila a iʻole SMS. Eia naʻe, ināʻaʻole i hōʻiliʻiliʻia ka pākaukau i loko o ka manawa i kohoʻia, a loaʻa paha kekahi mau maʻi a iʻole ka maʻi e like me ka hopena, e mālamaʻia ka mea kūʻai aku. Mahalo nui mākou iāʻoe e hōʻiliʻili i kāu uku wikiwiki e pale i nā pilikia kūpono.
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