Pichia Fasto HCP (Mai watsa shiri ta sel)
Pichia Fasto HCP (Mai watsa shiri ta sel)
✓ Pichia Fastos - Buɗeo - Babu giciye - Rasuwa tare da Cho / HCPS
✓ Shirya - Don amfani - Babu Samfurin Samfurin Sample da ake buƙata
Amfani da aka yi niyya
Wannan kit ɗin an tsara shi ne don abubuwan ganowar kayan aikin sel (HCP) a cikin kayayyakin biopharmaceutical da aka samo daga Pichia Fastos Strinis (E.G., SP33), ta amfani da wani guda - Mataki Enzenme - An haɗa shi da ingantaccen assay (Elisa). Don amfani kawai. Ba don dalilai na bincike ba.
Ka'idar ganowa
Kit ɗin yana aiki a sandwich Elis Hanyar:
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Shafi: Ickforging riplate an pre - mai rufi tare da rigakafin rigakafin rigakafin yaƙi da Pichia Fastos Hcps.
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Shiryuwa: Samfurori / an kara masu yawa, da HRP - Tashin garken tumaki na conjugated anti -Pichia Fastos Hcp rigakafin.
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Ganewa: Bayan wanke, tmm substrate an ƙara don ci gaban launi. An dakatar da amsawa, kuma ana auna shi a 450 nm.
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Ƙided: Ana lissafta maida hankali a hannun HCP daga daidaitaccen tsarin koli (tunawa α HCP taro).
Abubuwan da ke cikin key
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Babu Samfurin Samfurin Sample da ake buƙata (Tabbatarwar Dilove Duwatsu).
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Tsarin ladabi: Offin ƙananan matakai, sakamakon saurin (<2 hours).
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Babban bayani da amincin: Ingantawa don Pichia Fastos Hcps.
Bayanan kula da fasaha
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Samfuran samfurori masu jituwa: Saliban al'adu masu satar jama'a, kayan tarihin tsarkakakke.
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Range mai tsauri: 1-100 ng / ml (hali).
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Jagorar Kuɗi: Ya dace da ci gaban tsari da kuma gwajin da aka saki da aka saki.
Amfani da tsari1. Daidaitawa
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Kawo dukkan abubuwanda aka gyara don zafin jiki na daki (≥2 min).
2. Sample / antibody ƙari-
Sanya Samfurori / Kaliban: 100 μL / WA
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Sanya 1x enzyme - conjugated antibody: 100 μL / WA (ga Legend ①)
3. Shiryawa-
Acubate a Rt, kariya daga haske, 600 rpm don 3 h (ga Legend ③)
4. Wanke-
Wanke 1: 300 μL / da kyau 1x buffer (Matsayi mai tsaye)
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A wanke 2 - 5: 300 μL / da kyau 1x buffer, 5 duka wanke
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Patry bushe: Bayan wanka na ƙarshe, insty plate a kan takarda masu narkewa
5. TMB Substate ci gaba-
Ƙara tmb: 100 μL / WA
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Ci gaba: 10 min a rt (kariya daga haske)
6. Dakatar da dauki & sake-
Addara tsira: 50 μL / Daidai
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Tsaya: 5 min
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Karanta: OD A a 450 nm (ma'anar 620 nm)
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| Kayan wucin gadi | Samfurin A'a. | Gwadawa | Siffantarwa |
|---|---|---|---|
| Pre - mai rufi anti - pichia hcp microplate | Pna015 | 8 rijiyoyin × 12 stris | Pre - coated tare da tumaki polyllal anti -Pichia Fastos Hcp rigakafin. An hatimce a cikin tsare mai cike da ɓarna. Adana kariya daga haske. |
| Pichia HCP Calibrator | Pnb015 | 2 Vials | Lyophilized foda. Sake zama tare da 500 μL Calibtor Diluent (PNC002). Bada izinin 5-10 min don rushewa (mafita yakamata a bayyane). Adana kariya daga haske. |
| Calibtor Dilarent | Pnn2 | 1.5 ml × 1 bututu | Share bayani don sake fasalin Pichia HCP. |
| Assayi dillalient | Ne006 | 25 ml × 2 kwalabe | Don diluting calibrators, samfurori, da enzyme - conjugated antibody. Tabbatar da ingantaccen diliyya ga sababbin samfurori. |
| Wanke Buffer (10 × mai da hankali) | Pnf001 | 25 ml × 2 kwalabe | Don farantin farantin. Lu'ulu'u na iya zama a yanayin zafi-narke a 37 ° C kafin amfani. Tsara 10 × tare da ruwa mai ɗumbin ruwa. |
| HRP - Conjugated anti - pichia hcp antiby (100 ×) | Pnn009 | 120 μL × 1 bututu | Tumaki polyllonal antibody. Tsarma 100 × tare da assay diluhient kafin amfani. Adana kariya daga haske. |
| Tm substrate | Pnd004 | 12 Ml × 1 kwalban | Daidaita zuwa zazzabi dakin (≥2 min) kafin amfani. Adana kariya daga haske. |
| Tsaya bayani | Pni002 | 6 kwalban 1 | Ya ƙunshi HCL. Saka kafafu da gujewa lambar fata. |
| Sawun fina-finai | PNK001 | 3 zanen gado | Don murfin microplate tube don hana gurbatawa da ruwa. |
Bayanin jigilar kaya
Muna ba da sufuri mai sanyaya a kan dukkan umarni. Yawanci, odar ku zai zo tsakanin 5 - 7 kwanakin kasuwanci a Amurka kuma cikin kwanakin kasuwanci 10 na wasu ƙasashe. Koyaya, Lura cewa isar da yankunan karkara na iya ɗaukar ɗan lokaci kaɗan.
Lokaci na jigilar kaya: Ana amfani da umarni a cikin 1 - kwanaki 3. Da zarar an tura oda, za ku karɓi imel ɗin tabbatarwa tare da bayanin bin diddigin.
Bayani mai mahimmanci
Umarni aiki: Bayan an biya shi, an biya shi, shagon mu yana buƙatar ɗan lokaci don aiwatar da odarka. Za ku sami sanarwar da zarar an tura oda.
Times Isti: A mafi yawan lokuta, za a kawo kunshin a cikin lokacin isowa. Koyaya, ainihin ranar isar da kai zata iya shafar shi ta hanyar jirgin, yanayin yanayi, da sauran dalilai na waje. Tsarin isar da lokaci zai yi tsayi fiye da yadda aka saba don umarni waɗanda suka haɗa da preorder ko abubuwa musamman. Da fatan za a koma zuwa bayanin bin diddigin ranar isar da sako.
Batutuwan sufuri: Idan kun ga cewa ba a isar da kunshin ku a cikin ƙayyadadden lokacin ba; Bayanin sa ido ya nuna cewa an kawo kunshin amma ba ku karbe shi ba; Ko kunshin ku ya haɗa da abubuwan da aka ɓace ko ba daidai ba ko wasu batutuwa na abokin ciniki, don Allah a tuntuɓi sabis na abokin ciniki a cikin kwanaki 7 na ranar biyan kuɗi don mu iya magance waɗannan batutuwan da sauri.
Oda bibiya
Da zarar an tura oda, za ku karɓi imel tare da lambar sa ido da hanyar haɗi don bin tafarkinka.
Hakanan zaka iya bi oda kai tsaye akan rukunin yanar gizon mu ta hanyar shiga cikin asusunka da duba tarihin odar ka.
Arewararrawar jigilar kaya
Da fatan za a cika adireshin titi daki-daki, ba akwatin PO ba ko adireshin soja (APO). In ba haka ba, dole ne mu yi amfani da EMS don bayarwa (yana da hankali fiye da wasu, yana ɗaukar 1 - watanni 2 ko ma ya fi tsayi.
Ayyukan kwastomomi da manufofin haraji
Lura cewa duk wani aikin kwastomomi, ko haraji, ko kudaden shigowa da aka jawo a lokacin jigilar kaya sune alhakin mai siye ne. Wadannan tuhume-tuhume sun banbanta dangane da ƙasar da za ta isa kuma sun ƙaddara ta hukumomin kwastam na gida.
Ta hanyar siye daga shafin yanar gizon mu, kun yarda ku biya kowane irin aiki ko haraji mai alaƙa da odarka. Ba mu da alhakin jin jinkirin da aka haifar ta hanyar kwastan kwastam.
Packaddamar da Packpup
Da zarar umarnin ku ya isa wurin wasan da aka tsara ko wurin bayarwa, da fatan za a tabbatar da tarin gaggawa. Idan ba a tsinta kunshin a cikin lokacin da aka tsara ba, za mu aika tunatarwa ta imel ko SMS. Koyaya, idan ba a tattara kunshin a cikin ƙayyadadden lokaci ba, da kowane rashi ko lalacewa na faruwa a sakamakon, za a riƙe mai siye da mai siye. Muna tuna ku da fatan ku tattara kunshin ku da sauri don guje wa duk wasu manyan al'amura.
SAURARA: Kamar yadda samfurinmu ya faɗi ƙarƙashin rukuni na musamman, dawowa da kuɗi ba a karɓa ba.
